BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3662.p (465 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cgl0416 [GEPR] COG0477 Permeases of the major facilitator superf... 320 6e-87 STM0212 [GEPR] COG0477 Permeases of the major facilitator superf... 241 3e-63 ECs2778 [GEPR] COG0477 Permeases of the major facilitator superf... 224 4e-58 shiA [GEPR] COG0477 Permeases of the major facilitator superfami... 223 8e-58 Cj0339 [GEPR] COG0477 Permeases of the major facilitator superfa... 223 1e-57 >Cgl0416 [GEPR] COG0477 Permeases of the major facilitator superfamily Cgl Corynebacterium glutamicum Length = 467 Score = 320 bits (819), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 176/428 (41%), Positives = 263/428 (61%), Gaps = 6/428 (1%) Query: 11 QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFP-KGDPTTAIIASLATYGVGYVA 69 + K+AA S WIGSALEYYDF +Y TAA+L+ LFFP P AI+A++ T GV YV Sbjct: 17 KTPKRAALSSWIGSALEYYDFAVYGTAAALVLNHLFFPADTSPGIAILAAMGTVGVAYVV 76 Query: 70 RPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVLLRLAQG 129 RP+GA ++G LGD +GRK VL+ C+FL+G ST VG LPT+DQVG APALLVL R+ QG Sbjct: 77 RPLGALIMGPLGDRYGRKFVLMLCLFLIGASTFAVGCLPTFDQVGYLAPALLVLCRVIQG 136 Query: 130 FAVAGEISGASSMILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAHYMPTEQFNVW 189 + +GE S A S+ LEH+ R + +S+ L G Q G +LA VF+P ++ + W Sbjct: 137 LSASGEQSSAISVSLEHADERHRAFTASWTLHGTQFGTLLATGVFIPFTLFLSEDALMSW 196 Query: 190 GWRIPFLLSFLVIIVCWIIRRKVDETPVFKAADQQELKRRSPVVEAFRDCTPNMLRVVCM 249 GWR+PF LS V++V ++IRR ++E P F+ + SP+ R + RV Sbjct: 197 GWRVPFWLSAAVVLVAFLIRRGLEEPPAFRENKEAVAGAASPLAMTLRYHKAAVARVAIA 256 Query: 250 ALMNVIPVVATIFGAAYAVQPAYGVGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIGRR 309 A++N + +V T++ ++A VG + L +PVV N+VA++ IP G L+DRIGRR Sbjct: 257 AMINSVNIVFTVWALSFATNI---VGLDRSTVLLVPVVANLVALIAIPLSGMLADRIGRR 313 Query: 310 LPIVVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFFPELFPARA 369 ++G++ GL YL AI N + F M +LM G++Y NAV+P+F+ E+FP Sbjct: 314 PVFIMGAIGGGLAMNGYLGAIYSGNWTMIFFMGVLMSGLLYSMGNAVWPAFYAEMFPTSV 373 Query: 370 RVSAMAIGQNLGTAIT-AMLPALFTAVAPPGASHIWLTVGSIAFGITIVAALAAMSARET 428 RV+ +A+G +G A++ +P + +A+A W+ V + +++AL AM+A+ET Sbjct: 374 RVTGLALGTQIGFAVSGGFVPVIASALAGDQGDQ-WMKVSIFVGVVCVISALVAMTAKET 432 Query: 429 HRIPLEKL 436 + L+++ Sbjct: 433 KALTLDEI 440 >STM0212 [GEPR] COG0477 Permeases of the major facilitator superfamily Sty Salmonella typhimurium LT2 Length = 433 Score = 241 bits (615), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 147/430 (34%), Positives = 228/430 (53%), Gaps = 11/430 (2%) Query: 6 TNDHTQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGV 65 + + ++ S WIG+++EYYDF +Y A++L+F LFFP P TA ++S A++GV Sbjct: 2 SGTYNATIRRVVVSAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGV 61 Query: 66 GYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVLLR 125 G+++RP+GA G+ GDT GRK LL + MG T +G LP+Y +G APALLV+LR Sbjct: 62 GFISRPLGALFFGNRGDTLGRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILR 121 Query: 126 LAQGFAVAGEISGASSMILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAHYMPTEQ 185 QGF V GE GA M++E++ RG S+ + G GQ+LA VF+ + +P E Sbjct: 122 FLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLTGQLLATGVFIVVTQ-LPQEA 180 Query: 186 FNVWGWRIPFLLSFLVIIVCWIIRRKVDETPVFKAADQQEL------KRRSPVVEAFRDC 239 WGWRIPFLLS L+++ +R ++DETPVF+A +Q+ + PVV+ R+ Sbjct: 181 LLSWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAINHRQQREERPVVKVVREQ 240 Query: 240 TPNMLRVVCMALMNVIP-VVATIFGAAYAVQPAYGVGFAKDVYLWIPVVGNIVAVLVIPY 298 ++L ++ + +P +AT+F ++A +G A L+I + ++A + Sbjct: 241 WRSILLIIILRFAESVPFFLATVFAVSWATTQ---LGIASLTILYIVMFTCLLAYPMHVL 297 Query: 299 VGKLSDRIGRRLPIVVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFP 358 G +SDR G R + G+L AF + + + R++ L + +L+ + + NAV P Sbjct: 298 FGIMSDRRGCRQVYIFGALFVAAMAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQP 357 Query: 359 SFFPELFPARARVSAMAIGQNLGTAITAMLPALFTAVAPPGASHIWLTVGSIAFGITIVA 418 SFF LF R S +IG LG + + W V + + Sbjct: 358 SFFAGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDNSWALVAGYVVLTALAS 417 Query: 419 ALAAMSARET 428 A AA A ET Sbjct: 418 AFAAKIAPET 427 >ECs2778 [GEPR] COG0477 Permeases of the major facilitator superfamily Ecs Escherichia coli O157:H7 Length = 438 Score = 224 bits (570), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 127/434 (29%), Positives = 232/434 (53%), Gaps = 9/434 (2%) Query: 3 SSHTNDHT---QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIAS 59 S+ ++ T ++++AA + G+ +++YDF +Y A+L+F + FFP+ P +A+ Sbjct: 7 STRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAA 66 Query: 60 LATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPA 119 AT+GVG++ RP+G + GH GD GRK++L+ +++MG +T +G+LP++ +G WAP Sbjct: 67 FATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPI 126 Query: 120 LLVLLRLAQGFAVAGEISGASSMILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAH 179 LLV LR QGFAV GE GA+ + +E +P ++ +YSS G G +L+ + ++ Sbjct: 127 LLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKAFYSSGVQVGYGVGLLLSTGLVSLISM 186 Query: 180 YMPTEQFNVWGWRIPFLLSFLVIIVCWIIRRKVDETPVFKAA--DQQELKRRSPVVEAFR 237 EQF WGWRIPFL S ++++ +R ++E+ F+ +Q K+R PV+EA Sbjct: 187 MTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQAAAKKRIPVIEALL 246 Query: 238 DCTPNMLRVVCMALMNVIPV-VATIFGAAYAVQPAYGVGFAKDVYLWIPVVGNIVAVLVI 296 L+++ + L ++ + + T F Y+ Q +G ++++L I ++ ++ L I Sbjct: 247 RHPGAFLKIIALRLCELLTMYIVTAFALNYSTQ---NMGLPRELFLNIGLLVGGLSCLTI 303 Query: 297 PYVGKLSDRIGRRLPIVVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAV 356 P L+DR GRR + G+L+ L AF + A+ +++ SI++ + + V Sbjct: 304 PCFAWLADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCV 363 Query: 357 FPSFFPELFPARARVSAMAIGQNLGTAITAMLPALFTAVAPPGASHIWLTVGSIAFGITI 416 F E+F A R S +G + + + A + W +V + Sbjct: 364 QQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCL 423 Query: 417 VAALAAMSARETHR 430 ++A+ A+ ++ R Sbjct: 424 ISAMTALLMKDNQR 437 >shiA [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 438 Score = 223 bits (568), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 125/423 (29%), Positives = 227/423 (53%), Gaps = 6/423 (1%) Query: 11 QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVAR 70 ++++AA + G+ +++YDF +Y A+L+F + FFP+ P +A+ AT+GVG++ R Sbjct: 18 SRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFATFGVGFLFR 77 Query: 71 PIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVLLRLAQGF 130 P+G + GH GD GRK++L+ +++MG +T +G+LP++ +G WAP LLV LR QGF Sbjct: 78 PLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLVTLRAIQGF 137 Query: 131 AVAGEISGASSMILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAHYMPTEQFNVWG 190 AV GE GA+ + +E +P ++ +YSS G G +L+ + ++ EQF WG Sbjct: 138 AVGGEWGGAALLSVESAPKNKKAFYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWG 197 Query: 191 WRIPFLLSFLVIIVCWIIRRKVDETPVFKAAD--QQELKRRSPVVEAFRDCTPNMLRVVC 248 WRIPFL S ++++ +R ++E+ F+ Q K+R PV+EA L+++ Sbjct: 198 WRIPFLFSIVLVLGALWVRNGMEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIA 257 Query: 249 MALMNVIPV-VATIFGAAYAVQPAYGVGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIG 307 + L ++ + + T F Y+ Q +G ++++L I ++ ++ L IP L+DR G Sbjct: 258 LRLCELLTMYIVTAFALNYSTQ---NMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFG 314 Query: 308 RRLPIVVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFFPELFPA 367 RR + G+L+ L AF + A+ +++ SI++ + + V F E+F A Sbjct: 315 RRRVYITGTLIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGA 374 Query: 368 RARVSAMAIGQNLGTAITAMLPALFTAVAPPGASHIWLTVGSIAFGITIVAALAAMSARE 427 R S +G + + + A + W +V +++A+ A+ ++ Sbjct: 375 SYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD 434 Query: 428 THR 430 + R Sbjct: 435 SQR 437 >Cj0339 [GEPR] COG0477 Permeases of the major facilitator superfamily Cje Campylobacter jejuni Length = 453 Score = 223 bits (567), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 133/392 (33%), Positives = 210/392 (53%), Gaps = 10/392 (2%) Query: 2 QSSHTNDHTQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLA 61 ++ +T + + KKA S W+G+A+EY DF +Y AA+ IF ++FFP+ P A++ S Sbjct: 12 KNFNTPEGRKVFKKAVFSCWLGTAMEYADFALYGLAAATIFSEVFFPEQTPVIALLLSFV 71 Query: 62 TYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALL 121 TYG+G++ARPIGA GHLGD HGRK V++ + LMG ST +G +P+Y +G+WAP L Sbjct: 72 TYGIGFIARPIGALFFGHLGDKHGRKNVMMATIALMGISTTLIGFIPSYAVIGVWAPICL 131 Query: 122 VLLRLAQGFAVAGEISGASSMILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAHYM 181 V LR QGF E+SG + M+ E++P RRG SS G +G +LA+ V+L + M Sbjct: 132 VALRFMQGFGAGAELSGGTVMLAEYAPSKRRGLVSSVIALGSNSGTLLASFVWLLMVQ-M 190 Query: 182 PTEQFNVWGWRIPFLLSFLVIIVCWIIRRKVDETPVFKAADQQELKRRSPVVEAFRDCTP 241 F WGWR+PF+ S L+ + IR V ETPVF+ + +K R + + Sbjct: 191 DEASFKEWGWRVPFMGSILIALFAVYIRFHVKETPVFEKQKNEMMKLRLNNEKHMKKDER 250 Query: 242 NMLRVVCMALMNVIPVVATIFGAAYAVQPAYGVGFAKDVYLWIP-------VVGNIVAVL 294 + + A ++ + G +Y Q + VG+ + L ++ ++V L Sbjct: 251 SFWQ-RSRAFWTMVGLRIGENGPSYLAQ-GFIVGYVTKILLLDKSVATTAVMIASLVGFL 308 Query: 295 VIPYVGKLSDRIGRRLPIVVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYN 354 VIP G LSD+ GR + + LL ++AF + +N + + I+ + G Sbjct: 309 VIPLAGYLSDKFGRCITYRMFCLLLMIYAFPAFMLLDSKNEIIVILTIIVGMSLASLGIF 368 Query: 355 AVFPSFFPELFPARARVSAMAIGQNLGTAITA 386 V ++ ELF + R + MA + LG+ ++ Sbjct: 369 GVQAAWGVELFGVKNRYTKMAFAKELGSILSG 400