BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3483.p (487 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2211 [LKJ] COG0513 Superfamily II DNA and RNA helicases Rso R... 584 e-166 alr4718 [LKJ] COG0513 Superfamily II DNA and RNA helicases Nos N... 445 e-124 STM0820 [LKJ] COG0513 Superfamily II DNA and RNA helicases Sty S... 442 e-124 rhlE [LKJ] COG0513 Superfamily II DNA and RNA helicases Eco Esch... 440 e-123 ZrhlE [LKJ] COG0513 Superfamily II DNA and RNA helicases EcZ Esc... 440 e-123 >RSc2211 [LKJ] COG0513 Superfamily II DNA and RNA helicases Rso Ralstonia solanacearum Length = 495 Score = 584 bits (1506), Expect = e-166, Method: Compositional matrix adjust. Identities = 310/498 (62%), Positives = 359/498 (72%), Gaps = 18/498 (3%) Query: 3 DTTSASAAPAAPAVRFEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQT 62 +T + SAAP++ V F+ FGL PDIL+ALAE GY TPIQA AIPVV+ GRDVMGAAQT Sbjct: 2 NTETTSAAPSSDPVTFDTFGLHPDILRALAESGYTRATPIQAAAIPVVIAGRDVMGAAQT 61 Query: 63 GTGKTAGFSLPIIQRLLAHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLR 122 GTGKTAGFSLPIIQ LL A+ SASPARHPVRALILTPTRELADQV DNVA Y + T LR Sbjct: 62 GTGKTAGFSLPIIQNLLPDANTSASPARHPVRALILTPTRELADQVYDNVAKYAKHTALR 121 Query: 123 STVVFGGVDMAPQTAILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRMLDMG 182 S VVFGGVDM PQT LR GVE+++ATPGRLLDHVQQ++VNLSQ ++LV+DEADRMLDMG Sbjct: 122 SAVVFGGVDMNPQTEQLRRGVEVLVATPGRLLDHVQQRSVNLSQVRMLVLDEADRMLDMG 181 Query: 183 FLPDLQRIINLLPKQRQNLLFSATFSPEIKKLAASFQNNPVTIEVARSNATAENVTQTIY 242 FLPDLQRIINLLP RQ LLFSATFSPEIKKLAAS+ +P TIEVARSNATA+NV Q IY Sbjct: 182 FLPDLQRIINLLPAHRQTLLFSATFSPEIKKLAASYLRHPQTIEVARSNATADNVRQVIY 241 Query: 243 KVEEAAKADAVSFIIRQRELK----QVIVFSNTKIGASRLARTLLAEGVKASAIHGDKTQ 298 V + K A+ ++RQR + Q IVFSN+KIG SRLAR L E + A+AIHGDKTQ Sbjct: 242 TVPDGHKQAALVHLLRQRAEQGLPSQCIVFSNSKIGCSRLARALEREKINANAIHGDKTQ 301 Query: 299 SERMAALEAFKQGQIEVLVATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGA 358 +ERM LEAFKQG ++VLVATDVAARGLDI+++PCVIN+DLP+NAEDYVHRIGRTGRAGA Sbjct: 302 TERMQTLEAFKQGTVDVLVATDVAARGLDISQMPCVINFDLPFNAEDYVHRIGRTGRAGA 361 Query: 359 SGDAISLFCDKDERLLVDIEKLIKKKFERAELSGFAPRARHERSERSERSERGERSERGE 418 SGDA+SLF DERLL DIEKLIK+ R L+ F P R + Sbjct: 362 SGDALSLFAPGDERLLADIEKLIKRNLPRETLTDFDPTGEQARERERRERDERRNRTEIR 421 Query: 419 RGERSDRGERSRSSREGGAPAARREKIDPWFLKPYEPSTTETVST---------KPELAK 469 R D +R R DP+F +PYE + + P AK Sbjct: 422 RARERDERNGARVLDHTVVRPPFRPSEDPFFSRPYESNVPAEAAEAAKPAPAGGHPRSAK 481 Query: 470 NNRPKPKVAALLGGMPKR 487 RP +AALLGG+P++ Sbjct: 482 --RP---IAALLGGVPRK 494 >alr4718 [LKJ] COG0513 Superfamily II DNA and RNA helicases Nos Nostoc sp. PCC 7120 Length = 426 Score = 445 bits (1145), Expect = e-124, Method: Compositional matrix adjust. Identities = 226/404 (55%), Positives = 288/404 (71%), Gaps = 7/404 (1%) Query: 18 FEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQR 77 F GLS +I+ A+ E GY PTPIQ Q+IP VL GRD++ AQTGTGKTA F+LP++ Sbjct: 3 FSHLGLSNEIINAVTELGYTKPTPIQMQSIPAVLSGRDLLAGAQTGTGKTASFTLPLLHL 62 Query: 78 LLAHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLRSTVVFGGVDMAPQTA 137 L + SAS A P+RALILTPTRELA QV +V Y ++ L S V+FGGV + PQ Sbjct: 63 LSQDSLKSASNASSPIRALILTPTRELAAQVESSVRDYGKYLKLNSMVMFGGVSINPQKQ 122 Query: 138 ILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRMLDMGFLPDLQRIINLLPKQ 197 L+ V+I++ATPGRLLDHVQQ TVNLSQ +ILV+DEADRMLDMGF+ D++RI++LLPKQ Sbjct: 123 RLKGRVDILVATPGRLLDHVQQGTVNLSQIEILVLDEADRMLDMGFIRDIRRILSLLPKQ 182 Query: 198 RQNLLFSATFSPEIKKLAASFQNNPVTIEVARSNATAENVTQTIYKVEEAAKADAVSFII 257 RQNLLF ATFS +IK+LAA N P IEVAR N TA+ VTQ +YK+E K D ++ +I Sbjct: 183 RQNLLFFATFSDKIKELAAGLLNRPQMIEVARRNVTADTVTQKVYKIERDRKRDLLAHLI 242 Query: 258 RQRELKQVIVFSNTKIGASRLARTLLAEGVKASAIHGDKTQSERMAALEAFKQGQIEVLV 317 R+ QV+VF+ TK GA RL + L E ++A AIHG+K+QS R AL FK G ++VLV Sbjct: 243 RKDNWYQVLVFTRTKYGADRLVKQLGHERIQALAIHGNKSQSARTHALAKFKNGSLQVLV 302 Query: 318 ATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGASGDAISLFCDKDERLLVDI 377 ATD+AARGLDI+ELP V+N+DLPY EDYVHRIGRTGRAGASG+A+SL + LL DI Sbjct: 303 ATDIAARGLDISELPYVVNFDLPYVPEDYVHRIGRTGRAGASGEAVSLVSADEYHLLADI 362 Query: 378 EKLIKKKFERAELSGFAPRA-------RHERSERSERSERGERS 414 EKLI+K+ ++G + + ER ++ + S+ RS Sbjct: 363 EKLIEKRLPFELVAGIGANSQAKPEPTQDERKQKPKDSQHQPRS 406 >STM0820 [LKJ] COG0513 Superfamily II DNA and RNA helicases Sty Salmonella typhimurium LT2 Length = 454 Score = 442 bits (1137), Expect = e-124, Method: Compositional matrix adjust. Identities = 217/378 (57%), Positives = 279/378 (73%), Gaps = 2/378 (0%) Query: 18 FEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQR 77 F+ GL+PDIL+A+AEQGY PTPIQ QAIP VL+GRD+M +AQTGTGKTAGF+LP++Q Sbjct: 3 FDSLGLNPDILRAIAEQGYREPTPIQQQAIPAVLEGRDLMASAQTGTGKTAGFTLPLLQH 62 Query: 78 LLAHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLRSTVVFGGVDMAPQTA 137 L+ H H+ R PVRALILTPTRELA Q+ +NV Y ++ +RS VVFGGV + PQ Sbjct: 63 LITHQPHAK--GRRPVRALILTPTRELAAQIGENVRDYSKYLNIRSLVVFGGVSINPQMM 120 Query: 138 ILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRMLDMGFLPDLQRIINLLPKQ 197 LR GV++++ATPGRLLD Q V L Q +ILV+DEADRMLDMGF+ D++R++ LP + Sbjct: 121 KLRGGVDVLVATPGRLLDLEHQNAVKLDQIEILVLDEADRMLDMGFIHDIRRVLAKLPAK 180 Query: 198 RQNLLFSATFSPEIKKLAASFQNNPVTIEVARSNATAENVTQTIYKVEEAAKADAVSFII 257 RQNLLFSATFS +IK LA NP+ IEVAR N +E VTQ ++ V++ K + +S +I Sbjct: 181 RQNLLFSATFSDDIKALAEKLLRNPLEIEVARRNTASEQVTQHVHFVDKKRKRELLSQMI 240 Query: 258 RQRELKQVIVFSNTKIGASRLARTLLAEGVKASAIHGDKTQSERMAALEAFKQGQIEVLV 317 Q +QV+VF+ TK GA+ LA L +G++++AIHG+K+Q R AL FK G I VLV Sbjct: 241 GQGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIHGNKSQGARTRALADFKSGDIRVLV 300 Query: 318 ATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGASGDAISLFCDKDERLLVDI 377 ATD+AARGLDI ELP V+NY+LP EDYVHRIGRTGRA A+G+A+SL C + +LL DI Sbjct: 301 ATDIAARGLDIEELPHVVNYELPNVPEDYVHRIGRTGRAAATGEALSLVCVDEHKLLRDI 360 Query: 378 EKLIKKKFERAELSGFAP 395 EKL+KK+ R G+ P Sbjct: 361 EKLLKKEIPRITTPGYEP 378 >rhlE [LKJ] COG0513 Superfamily II DNA and RNA helicases Eco Escherichia coli K12 Length = 454 Score = 440 bits (1132), Expect = e-123, Method: Compositional matrix adjust. Identities = 215/378 (56%), Positives = 280/378 (74%), Gaps = 2/378 (0%) Query: 18 FEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQR 77 F+ GLSPDIL+A+AEQGY PTPIQ QAIP VL+GRD+M +AQTGTGKTAGF+LP++Q Sbjct: 3 FDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMASAQTGTGKTAGFTLPLLQH 62 Query: 78 LLAHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLRSTVVFGGVDMAPQTA 137 L+ H+ R PVRALILTPTRELA Q+ +NV Y ++ +RS VVFGGV + PQ Sbjct: 63 LITRQPHAK--GRRPVRALILTPTRELAAQIGENVRDYSKYLNIRSLVVFGGVSINPQMM 120 Query: 138 ILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRMLDMGFLPDLQRIINLLPKQ 197 LR GV++++ATPGRLLD Q V L Q +ILV+DEADRMLDMGF+ D++R++ LP + Sbjct: 121 KLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADRMLDMGFIHDIRRVLTKLPAK 180 Query: 198 RQNLLFSATFSPEIKKLAASFQNNPVTIEVARSNATAENVTQTIYKVEEAAKADAVSFII 257 RQNLLFSATFS +IK LA +NP+ IEVAR N ++ VTQ ++ V++ K + +S +I Sbjct: 181 RQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASDQVTQHVHFVDKKRKRELLSHMI 240 Query: 258 RQRELKQVIVFSNTKIGASRLARTLLAEGVKASAIHGDKTQSERMAALEAFKQGQIEVLV 317 + +QV+VF+ TK GA+ LA L +G++++AIHG+K+Q R AL FK G I VLV Sbjct: 241 GKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIHGNKSQGARTRALADFKSGDIRVLV 300 Query: 318 ATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGASGDAISLFCDKDERLLVDI 377 ATD+AARGLDI ELP V+NY+LP EDYVHRIGRTGRA A+G+A+SL C + +LL DI Sbjct: 301 ATDIAARGLDIEELPHVVNYELPNVPEDYVHRIGRTGRAAATGEALSLVCVDEHKLLRDI 360 Query: 378 EKLIKKKFERAELSGFAP 395 EKL+KK+ R + G+ P Sbjct: 361 EKLLKKEIPRIAIPGYEP 378 >ZrhlE [LKJ] COG0513 Superfamily II DNA and RNA helicases EcZ Escherichia coli O157:H7 EDL933 Length = 455 Score = 440 bits (1132), Expect = e-123, Method: Compositional matrix adjust. Identities = 215/378 (56%), Positives = 280/378 (74%), Gaps = 2/378 (0%) Query: 18 FEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQR 77 F+ GLSPDIL+A+AEQGY PTPIQ QAIP VL+GRD+M +AQTGTGKTAGF+LP++Q Sbjct: 3 FDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMASAQTGTGKTAGFTLPLLQH 62 Query: 78 LLAHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLRSTVVFGGVDMAPQTA 137 L+ H+ R PVRALILTPTRELA Q+ +NV Y ++ +RS VVFGGV + PQ Sbjct: 63 LITRQPHAK--GRRPVRALILTPTRELAAQIGENVRDYSKYLNIRSLVVFGGVSINPQMM 120 Query: 138 ILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRMLDMGFLPDLQRIINLLPKQ 197 LR GV++++ATPGRLLD Q V L Q +ILV+DEADRMLDMGF+ D++R++ LP + Sbjct: 121 KLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADRMLDMGFIHDIRRVLTKLPAK 180 Query: 198 RQNLLFSATFSPEIKKLAASFQNNPVTIEVARSNATAENVTQTIYKVEEAAKADAVSFII 257 RQNLLFSATFS +IK LA +NP+ IEVAR N ++ VTQ ++ V++ K + +S +I Sbjct: 181 RQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASDQVTQHVHFVDKKRKRELLSHMI 240 Query: 258 RQRELKQVIVFSNTKIGASRLARTLLAEGVKASAIHGDKTQSERMAALEAFKQGQIEVLV 317 + +QV+VF+ TK GA+ LA L +G++++AIHG+K+Q R AL FK G I VLV Sbjct: 241 GKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIHGNKSQGARTRALADFKSGDIRVLV 300 Query: 318 ATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGASGDAISLFCDKDERLLVDI 377 ATD+AARGLDI ELP V+NY+LP EDYVHRIGRTGRA A+G+A+SL C + +LL DI Sbjct: 301 ATDIAARGLDIEELPHVVNYELPNVPEDYVHRIGRTGRAAATGEALSLVCVDEHKLLRDI 360 Query: 378 EKLIKKKFERAELSGFAP 395 EKL+KK+ R + G+ P Sbjct: 361 EKLLKKEIPRIAIPGYEP 378