BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3445.p (208 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3665 [E] COG1280 Putative threonine efflux protein Pae Pseudom... 151 9e-37 mlr3188 [E] COG1280 Putative threonine efflux protein Mlo Mesorh... 108 8e-24 VC0136 [E] COG1280 Putative threonine efflux protein Vch Vibrio ... 102 4e-22 YPO3831 [E] COG1280 Putative threonine efflux protein Ype Yersin... 100 2e-21 RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralsto... 96 6e-20 >PA3665 [E] COG1280 Putative threonine efflux protein Pae Pseudomonas aeruginosa Length = 207 Score = 151 bits (382), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 103/201 (51%), Positives = 126/201 (62%), Gaps = 1/201 (0%) Query: 8 LFVPSCFALNMAFGPNNLLSMTIGAQHGLRPALLAAAGRLVAFALMIAIAALGMGALLMA 67 LFVP+CFALN+A GPNNLLS+ A+HG A LA GRL+AFA M+A+AA G+ +L Sbjct: 7 LFVPACFALNLAPGPNNLLSLNNAARHGFATASLAGGGRLLAFAGMLALAASGLALVLHT 66 Query: 68 SQIAFTVVKLLGAAYLVWLGCKIFFAKATVSAQGVELGRGGIRSFLRKEFLVAIGNPKAI 127 S F +KLLGAAYL+WL +++ A A R + R+EFLVA GNPKAI Sbjct: 67 SAWLFLAIKLLGAAYLLWLAVQLWRTDAQPLANEASPARPSLWRLGRQEFLVAAGNPKAI 126 Query: 128 LIFTAFFPQFVVPGNYWGS-FLLLGSIFLTLELVAIAAYAYAGTRLSGLLQNARGLRWMN 186 LIFTAF PQFV PG G+ F LG+ FL LE +AIA Y+YAG L LL R R N Sbjct: 127 LIFTAFLPQFVDPGQPLGAQFAQLGAAFLLLEWLAIALYSYAGLHLGRLLAGQRARRLFN 186 Query: 187 RISGGMMIVFGTILALARRPA 207 R ++ G L L+RRPA Sbjct: 187 RGCAALLGSAGLGLLLSRRPA 207 >mlr3188 [E] COG1280 Putative threonine efflux protein Mlo Mesorhizobium loti Length = 204 Score = 108 bits (270), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 73/206 (35%), Positives = 112/206 (54%), Gaps = 6/206 (2%) Query: 1 MPLDTLLLFVPSCFALNMAFGPNNLLSMTIGAQHGLRPALLAAAGRLVAFALMIAIAALG 60 M L+ +V +C + + GP L + +HG R L AG A+MIAI +G Sbjct: 1 MSLELYAAYVLACIVIILVPGPTVTLIIANSIRHGTRAGLANVAGTQAGLAIMIAIVGIG 60 Query: 61 MGALLMASQIAFTVVKLLGAAYLVWLGCKIFFAKATVSAQGV-ELGRGGIRSFLRKEFLV 119 + L+ F V+L+GAAYL+W+G ++F +K T++A G RGG F + LV Sbjct: 61 LNTLIAGMGHWFEWVRLIGAAYLIWMGVQMFRSKGTLNADGTARKPRGG---FFLQGLLV 117 Query: 120 AIGNPKAILIFTAFFPQFVVP-GNYWGSFLLLGSIFLTLELVAIAAYAYAGTRLSGLLQN 178 A+ NPK ++ F AFFPQF+ P GNY +++G + ++ + YA A R LL Sbjct: 118 ALSNPKTLVFFGAFFPQFIAPQGNYSLQIVVMGLTAMIFAAMSDSTYALAAGRAGRLLSA 177 Query: 179 ARGLRWMNRISGGMMIVFGTILALAR 204 +R ++ M+RISG ++ G LA +R Sbjct: 178 SR-IKLMSRISGSFLVGGGLWLAFSR 202 >VC0136 [E] COG1280 Putative threonine efflux protein Vch Vibrio cholerae Length = 205 Score = 102 bits (255), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 65/207 (31%), Positives = 114/207 (55%), Gaps = 6/207 (2%) Query: 1 MPLDTLLLFVPSCFALNMAFGPNNLLSMTIGAQHGLRPALLAAAGRLVAFALMIAIAALG 60 M + L ++ + ++A G + S++ G +G R +L A G + A I + +G Sbjct: 1 MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIG 60 Query: 61 MGALLMASQIAFTVVKLLGAAYLVWLGCKIFFAKA--TVSAQGVELGRGGIRSFLRKEFL 118 +GAL+ S +AFT++K +GAAYLVWLG + + +A T + EL + + LRK L Sbjct: 61 IGALVAQSALAFTLIKWIGAAYLVWLGIQKWRDRAPLTATTTSHELSQA---ALLRKAVL 117 Query: 119 VAIGNPKAILIFTAFFPQFVVPG-NYWGSFLLLGSIFLTLELVAIAAYAYAGTRLSGLLQ 177 + + NPK+I+ A FPQF+ P ++W FL+LG +T++ + + Y +L ++ Sbjct: 118 INLTNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAIVMFGYTALAAQLGRYIR 177 Query: 178 NARGLRWMNRISGGMMIVFGTILALAR 204 + + MN++ G M + G +LA A+ Sbjct: 178 SPNIMTRMNKLFGSMFMGCGMLLATAK 204 >YPO3831 [E] COG1280 Putative threonine efflux protein Ype Yersinia pestis Length = 206 Score = 100 bits (249), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 58/188 (30%), Positives = 107/188 (56%), Gaps = 6/188 (3%) Query: 21 GPNNLLSMTIGAQHGLRPALLAAAGRLVAFALMIAIAALGMGALLMASQIAFTVVKLLGA 80 G + +M+ HG R + + G + A+ I + +G+GAL+ S +AF ++K LGA Sbjct: 21 GSGAINTMSTAISHGTRGVVASIGGLQLGLAVHIVLVGVGLGALVSQSLLAFEILKWLGA 80 Query: 81 AYLVWLGCKIFFAKATVSAQGV--ELGRGGIRSFLRKEFLVAIGNPKAILIFTAFFPQFV 138 AYL+WLG + + A ++ + + R R ++ V + NPK+I+ A FPQFV Sbjct: 81 AYLIWLGIQQWRAAGSLDLHALANSMPR---RKLFKRAVFVNLTNPKSIVFLAALFPQFV 137 Query: 139 VPGN-YWGSFLLLGSIFLTLELVAIAAYAYAGTRLSGLLQNARGLRWMNRISGGMMIVFG 197 +P +L+LGS + ++++ + YA TR++ +++ + ++ +NRI GG+ ++ G Sbjct: 138 LPQQPQVAQYLILGSTSVIVDIIVMIGYATLATRIARWIKSPQQMKLLNRIFGGLFMLIG 197 Query: 198 TILALARR 205 +LA AR+ Sbjct: 198 ALLATARK 205 >RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralstonia solanacearum Length = 215 Score = 95.9 bits (237), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 66/208 (31%), Positives = 107/208 (51%), Gaps = 7/208 (3%) Query: 5 TLLLFVPSCFALNMAFGPNNLLSMTIGAQHGLRPALLAAAGRLVAFALMIAIAALGMGAL 64 L +F+ + LN+ GP+ + G L+++ G + + A G+ AL Sbjct: 8 NLPVFMLAVLLLNVTPGPDTAYIVGRSVSQGRAAGLMSSLGVSAGCCVHVLAVAFGLTAL 67 Query: 65 LMASQIAFTVVKLLGAAYLVWLGCKIFFAKATVS----AQGVELGRGGIRSFLRKEFLVA 120 L AS +AFTV+K +GAAYL++LG ++ A A AQ V R +R+ + FL Sbjct: 68 LAASAVAFTVIKAIGAAYLIYLGGRMLLAPAGSDDAPVAQAVATARRPLRALFMQGFLTN 127 Query: 121 IGNPKAILIFTAFFPQFVVPG--NYWGSFLLLGSIFLTLELVAIAAYAYAGTRLSGLLQN 178 + NPK +L F +FFPQFV PG + +FL+LG++F+ + + A+ ++ + Sbjct: 128 VLNPKVVLFFLSFFPQFVDPGASHKAAAFLVLGAVFIVMSTAWNSLVAWVAASVTRRVAG 187 Query: 179 ARGL-RWMNRISGGMMIVFGTILALARR 205 G+ RW++R+ G I G LA R Sbjct: 188 KPGIKRWLDRVVGTAFIGLGARLAFTTR 215