BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3384.p (567 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3792 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 746 0.0 RSp0322 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 729 0.0 Cgl0250 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 637 0.0 ML2324 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 628 e-180 mlr2792 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 627 e-179 >PA3792 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Pae Pseudomonas aeruginosa Length = 592 Score = 746 bits (1926), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/554 (64%), Positives = 451/554 (81%), Gaps = 3/554 (0%) Query: 2 MLSNPAAKYRPFPAIDLPDRTWPSKVISTPPIWMSTDLRDGNQALIEPMNAERKLRFFEL 61 ML +P+ KYRPF AI+LPDRTWPSK I+ PIW S+DLRDGNQ+LIEPM+A +K+RFF+ Sbjct: 39 MLKDPSQKYRPFSAINLPDRTWPSKTITEVPIWCSSDLRDGNQSLIEPMDAAKKMRFFKT 98 Query: 62 LLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSRDELIRRTVESLEGA 121 L++ GLK+IEV FPSAS TDF+FVR+LI N IPDDVTI VLTQ+R++LI RT ESL GA Sbjct: 99 LVQVGLKQIEVAFPSASDTDFNFVRELIEGNHIPDDVTIQVLTQAREDLITRTFESLRGA 158 Query: 122 KKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESFS 181 KKAIVH+YN+ AP+FR+IVFN ++ + +IA +L+K+L +P T+W F+YSPE FS Sbjct: 159 KKAIVHVYNATAPSFRRIVFNQDKQGVVDIATNAAKLIKKLAAEQPETQWSFQYSPEIFS 218 Query: 182 TTELDFSKEICDAVCETWGATPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRASTI 241 +TEL+FS E+C+AV + W TP+ K+ILNLP+TVECATPNVYADQIEW R++ R S I Sbjct: 219 STELEFSVEVCNAVIDVWQPTPDNKIILNLPATVECATPNVYADQIEWFGRHVDKRDSVI 278 Query: 242 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 301 IS+H HNDRGT VA+ EL +MAGADRVEGCLFGNGERTGNVDLVTLALN+YTQG++P LD Sbjct: 279 ISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTLALNMYTQGLHPQLD 338 Query: 302 FSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPYL 361 FSDID VR+VVEECNQ+PVHPRHPYVGDLV TAFSGSHQDAI+KGFA+Q+ DAIWEVPYL Sbjct: 339 FSDIDAVRKVVEECNQLPVHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQKEDAIWEVPYL 398 Query: 362 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGKE 421 PIDPAD+GR Y+AVIRVNSQSGKGG+ +LLEQEYG++LPRR+QIEFS+ +Q E D G E Sbjct: 399 PIDPADIGRDYEAVIRVNSQSGKGGITFLLEQEYGISLPRRMQIEFSQVVQGETDRLGLE 458 Query: 422 IAAADIHAIFQREYLERTEPYVYRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGNGP 481 + A I+++ + EYL+ T PYV +HR+ E++ + ++++EV +G+ RG G GP Sbjct: 459 MTAQQIYSLLENEYLKATSPYVLASHRLQEENGTS-AVDLEVSF--DGEKQHWRGIGKGP 515 Query: 482 IDAFVQALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFKA 541 ++A V AL + ++MD+HEHAIGAGA+AKAA+YIE+RL G+GID NI TASF+A Sbjct: 516 LEALVAALPVKAEIMDYHEHAIGAGANAKAAAYIEIRLEGQRPLHGIGIDENITTASFRA 575 Query: 542 VLSAVNRQIAISES 555 + SA+NR + +E+ Sbjct: 576 LFSALNRAVTQAEA 589 >RSp0322 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Rso Ralstonia solanacearum Length = 576 Score = 729 bits (1882), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/566 (61%), Positives = 435/566 (76%), Gaps = 8/566 (1%) Query: 2 MLSNPAAKYRPFPAIDLPDRTWPSKVISTPPIWMSTDLRDGNQALIEPMNAERKLRFFEL 61 ML +PA KYRPFP I LPDRTWPSK ++ PIWMSTDLRDGNQAL EPMNAERK+R F++ Sbjct: 1 MLKHPATKYRPFPPIALPDRTWPSKTLTRAPIWMSTDLRDGNQALFEPMNAERKMRMFKM 60 Query: 62 LLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSRDELIRRTVESLEGA 121 L++ G KEIE FP+ASQTD+DFVR+LI IPDDVTI VLTQ+R++LIRRT+ESL GA Sbjct: 61 LVQIGFKEIEAAFPAASQTDYDFVRELIEGGHIPDDVTIEVLTQAREDLIRRTMESLRGA 120 Query: 122 KKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESFS 181 ++AI+H+YN+ +P FR+ VFN RE +K IAV +L++E+ ++ P T+W ++YSPE FS Sbjct: 121 RRAIIHVYNATSPVFRRTVFNTDREGVKRIAVESAKLIREIAESMPETQWTYQYSPEVFS 180 Query: 182 TTELDFSKEICDAVCETWGATPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRASTI 241 TELDF+ E+C+AV E W TP K+I NLP+TVE ATPNVYADQIEWM R+L R S + Sbjct: 181 GTELDFALEVCNAVTEAWDPTPAHKIIFNLPATVEMATPNVYADQIEWMHRHLARRDSIL 240 Query: 242 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 301 +SVHPHNDRGTAVA+AELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLY+QG++PGLD Sbjct: 241 LSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYSQGIDPGLD 300 Query: 302 FSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPYL 361 FS ++ V + E+C Q+PVHPRHPYVGDLVFTAFSGSHQDAIKKGFA Q+PDAIWE+PYL Sbjct: 301 FSHVNDVARTCEDCTQLPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAVQKPDAIWEMPYL 360 Query: 362 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGKE 421 PIDPAD+GR+YD++IRVNSQSGKGG+AYLLE YG+A+PRRLQ+EFS +Q+ DA+G+E Sbjct: 361 PIDPADVGRTYDSIIRVNSQSGKGGVAYLLESGYGIAMPRRLQVEFSSTVQQLTDASGRE 420 Query: 422 IAAADIHAIFQREYLERTEPYVYRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGNGP 481 AADI A+FQ YL P Y +HR++E ++ I + V+I G GNGP Sbjct: 421 ATAADIWALFQETYLRADGPIGYVSHRLAERDDGSQHIRLVVNIADREH--VCEGVGNGP 478 Query: 482 ID----AFVQALGLDIKLMDFHEHAIGAGADAKAASYIELRL-NEAPTGFGVGIDANIVT 536 +D A L + + + E A+G GA+A A ++ EL A + FGVGIDAN+ T Sbjct: 479 LDALVQALAAVLAAPVSIHHYEERALGQGANADAIAFAELAAPGVAGSVFGVGIDANLTT 538 Query: 537 ASFKAVLSAVNRQIAISESANQAGSA 562 AS +AV+ VNR +A + Q G + Sbjct: 539 ASIRAVVGGVNR-LAARHAQAQPGQS 563 >Cgl0250 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Cgl Corynebacterium glutamicum Length = 616 Score = 637 bits (1643), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/581 (54%), Positives = 403/581 (69%), Gaps = 25/581 (4%) Query: 4 SNPAAKYRPFPA----IDLPDRTWPSKVISTPPIWMSTDLRDGNQALIEPMNAERKLRFF 59 S P +Y PF I LPDRTWP K I+ P W + DLRDGNQALI+PM+ ERK R F Sbjct: 35 SMPVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMF 94 Query: 60 ELLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSRDELIRRTVESLE 119 ELL++ G KEIEVGFPSASQTDFDFVR++I + IPDDVTI VL Q+R+ LIRRT E+ E Sbjct: 95 ELLVQMGFKEIEVGFPSASQTDFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACE 154 Query: 120 GAKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPES 179 GAK IVH YNS + R +VF M + ++K +A L+K + P T WR++YSPES Sbjct: 155 GAKNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIKTIAQDYPDTNWRWQYSPES 214 Query: 180 FSTTELDFSKEICDAVCETWGATPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRAS 239 F+ TE++++KE+ DAV E TPE +I+NLPSTVE TPNVYAD IEWM RNL R S Sbjct: 215 FTGTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDS 274 Query: 240 TIISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPG 299 I+S+HPHNDRGT V +AEL MAGADR+EGCLFGNGERTGNV LVTLALN+ TQGV+P Sbjct: 275 IILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQ 334 Query: 300 LDFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGF----AKQQP--- 352 LDF+DI +R VE CNQ+ V RHPY GDLVFTAFSGSHQDA+ KG AK QP Sbjct: 335 LDFTDIRQIRSTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGAS 394 Query: 353 ----------DAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRR 402 D WEVPYLPIDP D+GR Y+AVIRVNSQSGKGG+AY+++ ++GL +PR Sbjct: 395 STEVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRS 454 Query: 403 LQIEFSRAIQREADATGKEIAAADIHAIFQREYLERTEPYVYRAHRMSEDSSKAESINIE 462 +Q+EFS +Q DA G E+ + + IF EYLERT P A R+ ++ E +I Sbjct: 455 MQVEFSTVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASIT 514 Query: 463 VDIVRNGQPVTVRGSGNGPIDAFVQA---LGLDIKLMDFHEHAIGAGADAKAASYIELRL 519 +++ NG+ VTV G GNGP+ A+ A LG+D+++ ++++HA +G DA+AA+Y+ + Sbjct: 515 AELIHNGKDVTVDGRGNGPLAAYANALEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEV 574 Query: 520 NEAPTGFGVGIDANIVTASFKAVLSAVNRQIAISESANQAG 560 N +GVGI +I AS KAV SAVNR + ++ A AG Sbjct: 575 NGRKV-WGVGIAGSITYASLKAVTSAVNRALDVNHEAVLAG 614 >ML2324 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Mle Mycobacterium leprae Length = 607 Score = 628 bits (1620), Expect = e-180, Method: Compositional matrix adjust. Identities = 312/564 (55%), Positives = 400/564 (70%), Gaps = 18/564 (3%) Query: 4 SNPAAKYRPFPA----IDLPDRTWPSKVISTPPIWMSTDLRDGNQALIEPMNAERKLRFF 59 S P +YRPF I L DRTWP +VI P+W + DLRDGNQALI+PM+ K R F Sbjct: 43 SMPVFRYRPFAKEVEPIRLVDRTWPDRVIDCAPLWCAVDLRDGNQALIDPMSPICKRRMF 102 Query: 60 ELLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSRDELIRRTVESLE 119 +LL++ G KEIEVGFPSASQTDFDFVR++I + IPDDVTI VLTQ R ELI RT E+ E Sbjct: 103 DLLVRMGYKEIEVGFPSASQTDFDFVREIITDGTIPDDVTIQVLTQCRPELIERTFEACE 162 Query: 120 GAKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPES 179 A + IVH YNS + R++VF + +K IA G R E PGT WRFEYSPES Sbjct: 163 NASRVIVHFYNSTSILQRRVVFRADQATVKAIATDGARKCVEEAFKYPGTHWRFEYSPES 222 Query: 180 FSTTELDFSKEICDAVCETWGATPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRAS 239 ++ TEL+++K++CDAV E TP+ +I NLP+TVE ATPNVYAD IEWM RNL +R S Sbjct: 223 YTGTELEYAKQVCDAVGEVIQPTPDNPIIFNLPATVEMATPNVYADSIEWMSRNLANRES 282 Query: 240 TIISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPG 299 I+S+HPHNDRGTAVA+AEL AGADR+EGCLFGNGERTGNV LVTL LNL+++GV+P Sbjct: 283 VILSLHPHNDRGTAVAAAELGYHAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQ 342 Query: 300 LDFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQ-------- 351 +DFS+ID +R+ VE CN++ VH RHPY GDLV+TAFSGSHQDAI KG + + Sbjct: 343 IDFSNIDEIRRTVEYCNKLRVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADS 402 Query: 352 --PDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSR 409 D +W+VPYLPIDP D+GR+Y+AVIRVNSQSGKGG+AY+++ ++GL LPRRLQIEFSR Sbjct: 403 DVDDILWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLELPRRLQIEFSR 462 Query: 410 AIQREADATGKEIAAADIHAIFQREYLERTEPYVYRAHRMSEDSSKAESINIEVDIVRNG 469 AIQ+ ++ G EI ++ +F EYL +P R++ S +I + NG Sbjct: 463 AIQKISEGEGGEITPTEMWDVFFEEYLSPVQPLERIKQRVNAAEEDGGSTSIAATVKING 522 Query: 470 QPVTVRGSGNGPIDAFVQAL---GLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGF 526 + + G GNGP+ AF+ AL GL + ++D+ EHA+ AG DA+AA+Y+E ++ T + Sbjct: 523 EETEISGVGNGPLAAFIDALGHVGLQVAVLDYSEHAMNAGDDAQAAAYVEASVH-GHTAW 581 Query: 527 GVGIDANIVTASFKAVLSAVNRQI 550 GVGI +I TAS +AV+SAVNR + Sbjct: 582 GVGIAPSITTASLRAVVSAVNRAM 605 >mlr2792 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Mlo Mesorhizobium loti Length = 558 Score = 627 bits (1616), Expect = e-179, Method: Compositional matrix adjust. Identities = 311/558 (55%), Positives = 400/558 (71%), Gaps = 13/558 (2%) Query: 3 LSNPAAKYRPFPAIDLPDRTWPSKVISTPPIWMSTDLRDGNQALIEPMNAERKLRFFELL 62 + + A KY+P+P + L DRTWP+KVI PIW S DLRDGNQALI+PM ERK R F LL Sbjct: 1 MPDAARKYQPYPTVGLTDRTWPNKVIDKAPIWCSVDLRDGNQALIDPMGHERKARMFGLL 60 Query: 63 LKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSRDELIRRTVESLEGAK 122 L G KEIE+GFPSASQTDFDF R I E +P DV++ VL Q R ELI RT E+L+GA Sbjct: 61 LDMGFKEIEIGFPSASQTDFDFARWCIEEGNVPADVSLQVLVQCRPELITRTFEALKGAT 120 Query: 123 KAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESFST 182 IVH YNS + R++VF IK IA +++ ++ A+ G +RFEYSPESF+ Sbjct: 121 NPIVHFYNSTSELQRRVVFEKDVGGIKRIATDAAKMITDMA-AKAGGGYRFEYSPESFTG 179 Query: 183 TELDFSKEICDAVCETWGATPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRASTII 242 TEL+ + EIC+AV E P+ K+I+NLPSTVE +TPN+YAD+IEWMCRNL +R + II Sbjct: 180 TELEVALEICNAVTEIVRPKPDNKLIINLPSTVEMSTPNIYADRIEWMCRNLDNRENLII 239 Query: 243 SVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLDF 302 S+HPHNDRGT +A+ EL +MAGADRVEG LFGNGERTGNVD+VTLALN+YTQGV+PG+D Sbjct: 240 SLHPHNDRGTGIATTELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPGIDC 299 Query: 303 SDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFA--KQQPDAIWEVPY 360 SDI+ ++ V E NQ+ + RHPYVG+LV+TAFSGSHQDAI KG K+ ++WEVPY Sbjct: 300 SDINRMKDVYEYSNQLKIPERHPYVGELVYTAFSGSHQDAINKGMKALKKANTSLWEVPY 359 Query: 361 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGK 420 LPIDPAD+GRSY+A+IR+NSQSGKGG+AY+L+ +YGL LPR LQIEFS+AIQ DA GK Sbjct: 360 LPIDPADVGRSYEAIIRINSQSGKGGIAYVLQADYGLNLPRNLQIEFSQAIQAITDAEGK 419 Query: 421 EIAAADIHAIFQREYLERTEPYV-YRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGN 479 E+ A IH F Y+++ + + H D++ IE I+ G+ VT+ G+G Sbjct: 420 EVPAKRIHERFLETYVDQPGARLKFLDHHTYPDTAVKGRRVIEAVILDKGKEVTISGTGT 479 Query: 480 GPIDAFVQAL----GLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTG--FGVGIDAN 533 GPID FV AL G+++ ++D+ EH++ G++A A SY+E+ E P G FG GI+ N Sbjct: 480 GPIDGFVDALSRHVGVEMSVLDYSEHSMQRGSNASAISYVEM---EYPGGKLFGAGINTN 536 Query: 534 IVTASFKAVLSAVNRQIA 551 IV AS +AV SA NR + Sbjct: 537 IVAASLEAVTSAANRIVG 554