BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3311.p (255 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1597 [Q] COG0412 Dienelactone hydrolase and related enzymes Pa... 222 6e-58 PA1166 [Q] COG0412 Dienelactone hydrolase and related enzymes Pa... 103 3e-22 mll3556 [Q] COG0412 Dienelactone hydrolase and related enzymes M... 78 2e-14 alr1077 [Q] COG0412 Dienelactone hydrolase and related enzymes N... 54 4e-07 SSO2087 [Q] COG0412 Dienelactone hydrolase and related enzymes S... 53 5e-07 >PA1597 [Q] COG0412 Dienelactone hydrolase and related enzymes Pae Pseudomonas aeruginosa Length = 241 Score = 222 bits (565), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 120/243 (49%), Positives = 154/243 (63%), Gaps = 9/243 (3%) Query: 19 SELGIETIAHSADDIDYESVLLYNKT----RPQTSGVLTVPNYFGLRQQALEIAATTIGA 74 SE+ +E +A+ D YE L+Y+ + RP G+L PN+ G+ AL+IA G Sbjct: 2 SEIRVEPVAYDIDGQPYEGQLVYDASHAGPRP---GLLMAPNWMGVSAAALDIARQVAGR 58 Query: 75 GHAIFVADVFGKTLRPANPDQALAAIIPLKQDRAQLRKRMQAALQAFGQQSFVKIAADKF 134 GH + VAD++G+ +RP N D+A AA++PLK DRA LRKRMQAAL A Q+ + + Sbjct: 59 GHVVLVADLYGRDVRPQNGDEAGAAMMPLKNDRALLRKRMQAALAALRGQALAAVDTTRQ 118 Query: 135 GACGFCFGGTAALELARDGGEVRGTVSLHGALDTPAPATSK-VKGAVLVLHGVQDPSVPR 193 A GFCFGG ALELARDG E++ VS HG LDTP PA ++ +KGAVLVL G DP VPR Sbjct: 119 AAFGFCFGGCCALELARDGAELKAFVSFHGTLDTPDPAHARNIKGAVLVLDGASDPLVPR 178 Query: 194 EQVNGFIDEMSALKVDWQLVNYGQAGHSFTDPDANNPG-FFYHRQTAERAAVALQAFFGE 252 EQ+ F EM+ VDWQL +YG A HSFTDP+A PG Y +T+ RA A+ E Sbjct: 179 EQLPAFAREMTDAGVDWQLTSYGGAVHSFTDPNAKLPGKMHYDARTSRRAFQAMDDLLAE 238 Query: 253 VLA 255 V A Sbjct: 239 VFA 241 >PA1166 [Q] COG0412 Dienelactone hydrolase and related enzymes Pae Pseudomonas aeruginosa Length = 262 Score = 103 bits (258), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 71/233 (30%), Positives = 109/233 (46%), Gaps = 9/233 (3%) Query: 29 SADDIDYESVLLYNKTRPQTS-GVLTVPNYFGLRQQALEIAATTIGAGHAIFVADVFGKT 87 SAD Y+ ++P GV+ V ++GL A A G++ D++G+ Sbjct: 29 SADGTRMVGYFAYDDSKPGIRPGVIVVHEWWGLNDYAKRRARDLAELGYSALAIDMYGEG 88 Query: 88 LRPANPDQALAAIIPLKQDRAQLRKRMQAALQAFGQQSFVKIAADKFGACGFCFGGTAAL 147 +P A+A + +D + R A L+ +Q + + A G+CFGG L Sbjct: 89 KHTEHPQDAMAFMQAATRDADAAKARFLAGLELLKRQP--QTDPSQIAAIGYCFGGKIVL 146 Query: 148 ELARDGGEVRGTVSLHGALDTPAPATS-KVKGAVLVLHGVQDPSVPREQVNGFIDEMSAL 206 ++AR G + G S HGAL T PA+ VK +LV HG D VP + ++ E+SA Sbjct: 147 DMARQGLPLAGVASFHGALGTATPASKGSVKAKILVEHGSADSLVPAKDLDALKQELSAA 206 Query: 207 KVDWQLVNYGQAGHSFTDPDAN-----NPGFFYHRQTAERAAVALQAFFGEVL 254 D+++V A H FT+PDA+ Y RQ R+ LQAF ++ Sbjct: 207 GADYRVVIQDGAKHGFTNPDADAHKGHGLDIGYDRQADRRSWADLQAFLKDIF 259 >mll3556 [Q] COG0412 Dienelactone hydrolase and related enzymes Mlo Mesorhizobium loti Length = 244 Score = 77.8 bits (190), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 61/203 (30%), Positives = 93/203 (45%), Gaps = 6/203 (2%) Query: 51 VLTVPNYFGLRQQALEIAATTIGAGHAIFVADVFGKTLRPANPDQALAAIIPLKQDRAQL 110 ++ VP+++G+ L A G A+ VADV+G +R + QA A L+ + Sbjct: 40 LVYVPDWYGIYDYTLAEARRFASLGFAVMVADVYGAGVRITSDAQATQASQFLRDNPRTA 99 Query: 111 RKRMQAALQAFGQQSFVKIAADKFGACGFCFGGTAALELARDGGEVRGTVSLHGALDTPA 170 +R+QA ++AF V A A GF GG LE+ D VRG V L GAL PA Sbjct: 100 SRRLQAGVRAFRD---VVPQAPFLAAVGFSLGGFCVLEMLTDAPLVRGAVILSGALGQPA 156 Query: 171 PATSKVKGAVLVLHGVQDPSVPREQVNGFIDEMSALKVDWQLVNYGQAGHSFTDPDANNP 230 + ++ + HG +D +V + + A D L Y A H+FT+P+ + Sbjct: 157 GDYAAIETPIRFHHGTRDMVADVARVTKVLAWLEAKGCDCALTLYAGARHAFTNPNLPSG 216 Query: 231 G---FFYHRQTAERAAVALQAFF 250 G Y + A A A ++F Sbjct: 217 GDGWLGYDARYAGEADAATESFL 239 >alr1077 [Q] COG0412 Dienelactone hydrolase and related enzymes Nos Nostoc sp. PCC 7120 Length = 248 Score = 53.5 bits (127), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 43/162 (26%), Positives = 73/162 (45%), Gaps = 18/162 (11%) Query: 103 LKQDRAQLRKRMQAALQAFGQQSFVKIAADKFGACGFCFGGTAALELARDGGEVRGTVSL 162 ++ ++L +QAA+ ++ ++ + FG GFCFGG A LA +++ T S Sbjct: 93 MQTKASELLNDIQAAIDYL--KTLPQVKQNGFGCIGFCFGGHVAY-LAATLPDIKATASF 149 Query: 163 HGAL---------DTPAPATSKVKGAVLVLHGVQDPSVPREQVNGFIDEMSALKVDWQLV 213 +GA + T ++KG + G +D S+P EQ++ E+ + ++ Sbjct: 150 YGAGIATRTFGGGNPTVTRTPEIKGTLYGFFGTEDASIPPEQIDQIETELEKYNISHRVF 209 Query: 214 NYGQAGHS-FTDPDANNPGFFYHRQTAERAAVALQAFFGEVL 254 Y A H F D A+ Y+ Q A A +Q F VL Sbjct: 210 RYDGADHGFFCDRRAS-----YNPQAAADAWEQVQQLFKTVL 246 >SSO2087 [Q] COG0412 Dienelactone hydrolase and related enzymes Sso Sulfolobus solfataricus Length = 257 Score = 53.1 bits (126), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 40/124 (32%), Positives = 62/124 (50%), Gaps = 12/124 (9%) Query: 133 KFGACGFCFGGTAALELARDGGEVRGTVSLHGALDTPAPATSKVKGAVLVLHGVQDPSVP 192 K + GFC GG A +LA + + GT+ +G P A K+KG +L L+ +DP + Sbjct: 141 KIVSMGFCMGGGLAFQLATEV-PLDGTIVFYGRNPQPLEAIQKIKGPILGLYAGEDPPID 199 Query: 193 REQVNGFIDEMSAL---KVDWQLVNYGQAGHSFTDPDANNPGFFYHRQTAERAAVALQAF 249 G D +SA+ K D +L Y A H+F N+ G Y+++ AE A +++F Sbjct: 200 A----GLPDLISAIIKYKKDLELKIYPGAYHAFF----NDRGRSYNKEAAEDAWERVKSF 251 Query: 250 FGEV 253 V Sbjct: 252 LRRV 255