BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3271.p (455 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2025 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 491 e-138 BMEI0972 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase compl... 412 e-115 CC2302 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 410 e-114 all4968 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 402 e-112 mll0523 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 399 e-111 >PA2025 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Pae Pseudomonas aeruginosa Length = 451 Score = 491 bits (1263), Expect = e-138, Method: Compositional matrix adjust. Identities = 264/451 (58%), Positives = 323/451 (71%), Gaps = 5/451 (1%) Query: 4 YDYDLFTIGGGSGGVRAARFAGQAGARVGLAEKGELGGTCVNVGCIPKKLMSYSAHYHED 63 +D+DLF IG GSGGVRAARFA GARV +AE LGGTCVNVGC+PKKL+ Y AH+ ED Sbjct: 3 FDFDLFVIGAGSGGVRAARFAAGFGARVAVAESRYLGGTCVNVGCVPKKLLVYGAHFSED 62 Query: 64 FADAAGYGWTLHSQPSFDWVALIANKDREIAHLNDIYQRLLDGANVTLHRGFATVEDAHT 123 F A YGW+ + FDW LI NK+REI LN IY+ LL + VTL G A + DAH+ Sbjct: 63 FEQARAYGWS-AGEAQFDWATLIGNKNREIQRLNGIYRNLLVNSGVTLLEGHARLLDAHS 121 Query: 124 VNVDGQRFTARHILVATGGHPDKPQIAGSEWGITSDDFFHLPGLPQRAVVLGGGYIAVEL 183 V VDGQRF+A+HILVATGG P P I G E ITS++ F L LP+R +V+GGGYIAVE Sbjct: 122 VEVDGQRFSAKHILVATGGWPQVPDIPGKEHAITSNEAFFLERLPRRVLVVGGGYIAVEF 181 Query: 184 ASILNGLGSEVTLVYRRERLLRNMDADLGIHLADEMAKKGIRLIFNASIDAIEAAHAGPD 243 ASI NGLG+E TL+YRR+ LR D + HL DE+ KKG+ L FN+ I I+ G Sbjct: 182 ASIFNGLGAETTLLYRRDLFLRGFDRSVREHLRDELGKKGLDLQFNSDIARIDKQADG-- 239 Query: 244 SLKQVHLTNGETLAAECVLFATGRTPNTRGLGLEQAGVALKENGAVLVNDNFESSVPSIL 303 SL L +G L A+CV +ATGR P LGLE V L + G + V++++++S PSIL Sbjct: 240 SLAAT-LKDGRVLEADCVFYATGRRPMLDDLGLENTAVKLTDKGFIAVDEHYQTSEPSIL 298 Query: 304 AVGDVIDRVALTPVALAEAMAVVARLFGQGE-RSMSYRNIPTAVFSHPNVGTVGLSEEQA 362 A+GDVI RV LTPVALAE MAV RLF E R + Y+ IPTAVFS PN+GTVGL+EE+A Sbjct: 299 ALGDVIGRVQLTPVALAEGMAVARRLFKPEEYRPVDYKLIPTAVFSLPNIGTVGLTEEEA 358 Query: 363 RSEHGEVKIFKTDFKPLKNTLSRNTERTFMKLVVDAKSDRVLGVHMVGGDAGEVIQGFAV 422 S +VKIF++ F+P+K TL+ + E+T MKLVVDA DRVLG HMVG +AGE++QG AV Sbjct: 359 LSAGHKVKIFESRFRPMKLTLTDDQEKTLMKLVVDAHDDRVLGCHMVGAEAGEILQGIAV 418 Query: 423 ALQCGATKAQFDTTIGIHPTSAEEFVTLRTP 453 A++ GATK FD TIGIHPT+AEEFVTLRTP Sbjct: 419 AMKAGATKQAFDETIGIHPTAAEEFVTLRTP 449 >BMEI0972 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Bme Brucella melitensis Length = 483 Score = 412 bits (1060), Expect = e-115, Method: Compositional matrix adjust. Identities = 219/456 (48%), Positives = 308/456 (67%), Gaps = 7/456 (1%) Query: 1 MSQYDYDLFTIGGGSGGVRAARFAGQAGARVGLAEKGELGGTCVNVGCIPKKLMSYSAHY 60 M+ +DYDLF IGGGSGGVRA R AG G +VGLAE+ +GGTCV GC+PKKL Y++ + Sbjct: 20 MTGFDYDLFVIGGGSGGVRAGRLAGAIGKKVGLAEEYRMGGTCVIRGCVPKKLFVYASQF 79 Query: 61 HEDFADAAGYGWTLHSQPSFDWVALIANKDREIAHLNDIYQRLLDGANVTLHRGFATVED 120 E F D+AGYGW++ + +FDW LIA KD+EI+ L +Y++ L+ ++V + A + D Sbjct: 80 PEHFEDSAGYGWSM-GEAAFDWKKLIAAKDKEISRLEGLYRKGLENSHVDIFASRAELID 138 Query: 121 AHTVNV--DGQRFTARHILVATGGHPDKPQ-IAGSEWGITSDDFFHLPGLPQRAVVLGGG 177 HT+ + DG+R TA IL+ATGG P+ + + G E+ I+S++ FHL LP+ V+ GGG Sbjct: 139 EHTIELKADGRRVTADKILIATGGRPNTHESLPGHEYCISSNEAFHLEELPKSIVIAGGG 198 Query: 178 YIAVELASILNGLGSEVTLVYRRERLLRNMDADLGIHLADEMAKKGIRLIFNASIDAIEA 237 YIAVE A+I +GLG E TLVYR + +L D D+ L + M KGIR++ A + +E Sbjct: 199 YIAVEFANIFHGLGVETTLVYRGKEILSRFDHDIRSLLHETMEAKGIRILCGAIFEKVEK 258 Query: 238 AHAGPDSLKQVHLTNGETLAAECVLFATGRTPNTRGLGLEQAGVALKENGAVLVNDNFES 297 G S V L+NGETL + + A GR PNT GLGLE+AGV + ++GA+ V+D + Sbjct: 259 KADGKLS---VELSNGETLETDQAMLALGRIPNTDGLGLEKAGVKIDKSGAIEVDDYSRT 315 Query: 298 SVPSILAVGDVIDRVALTPVALAEAMAVVARLFGQGERSMSYRNIPTAVFSHPNVGTVGL 357 +V +I AVGDV +RV LTPVA+ EAM + F + I TAVFS P +GTVGL Sbjct: 316 NVENIWAVGDVTNRVQLTPVAIHEAMCFLETAFKDNPTKPDHELIATAVFSQPEIGTVGL 375 Query: 358 SEEQARSEHGEVKIFKTDFKPLKNTLSRNTERTFMKLVVDAKSDRVLGVHMVGGDAGEVI 417 SE++A ++ E++I++ F+P+KNTLS +E+T MKLVV++KS +VLG H++G DAGE+ Sbjct: 376 SEDEAAKKYPELEIYRALFRPMKNTLSGRSEKTLMKLVVESKSRKVLGAHIMGPDAGEMA 435 Query: 418 QGFAVALQCGATKAQFDTTIGIHPTSAEEFVTLRTP 453 Q ++L+ GATK FD T+ +HPT+ EE VT+ P Sbjct: 436 QLLGISLKAGATKDDFDRTMAVHPTATEELVTMYKP 471 >CC2302 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Ccr Caulobacter vibrioides Length = 466 Score = 410 bits (1053), Expect = e-114, Method: Compositional matrix adjust. Identities = 218/458 (47%), Positives = 287/458 (62%), Gaps = 11/458 (2%) Query: 1 MSQYDYDLFTIGGGSGGVRAARFAGQAGARVGLAEKGELGGTCVNVGCIPKKLMSYSAHY 60 M+ YD+DLF IG GSGGVRAAR A +GA+V +AE+ +GGTCV GC+PKK M Y++ Sbjct: 1 MADYDFDLFVIGAGSGGVRAARLAALSGAKVAVAEEYRVGGTCVVRGCVPKKFMVYASEV 60 Query: 61 HEDFADAAGYGWTLHSQPSFDWVALIANKDREIAHLNDIYQRLLDGANVTLHRGFATVED 120 A GYGWT+ FDW + KD EIA L+ IY L A L G A + D Sbjct: 61 TSQLKTAKGYGWTIEDA-RFDWKTFLHEKDVEIARLSGIYVTNLQKAGAHLLHGRAQIVD 119 Query: 121 AHTVNV-------DGQRFTARHILVATGGHPDKPQIAGSEWGITSDDFFHLPGLPQRAVV 173 AHTV V D +TAR ILVATGG P +P G+E GITSD+ FHLP LP+ +V Sbjct: 120 AHTVEVLPKDGSDDAGTYTARKILVATGGRPVRPVFPGAELGITSDEAFHLPTLPKSVLV 179 Query: 174 LGGGYIAVELASILNGLGSEVTLVYRRERLLRNMDADLGIHLADEMAKKGIRLIFNASID 233 +GGGYIAVE A I GLG + TL+YR +LR D D+ +HLADE+ K+GI+++ S Sbjct: 180 VGGGYIAVEFAGIYAGLGVQTTLLYRGANILRGFDDDVRMHLADELEKRGIKVVLGCSHK 239 Query: 234 AIEAAHAGPDSLKQVHLTNGETLAAECVLFATGRTPNTRGLGLEQAGVALKENGAVLVND 293 +IE D L+N T E V+FATGR P +GLGLE+AGV L + GA+ V+ Sbjct: 240 SIEKLD---DGRLLSTLSNDLTFETEAVMFATGREPYVQGLGLEKAGVKLNDKGAIAVDK 296 Query: 294 NFESSVPSILAVGDVIDRVALTPVALAEAMAVVARLFGQGERSMSYRNIPTAVFSHPNVG 353 +++V SI AVGDV DR+ LTPVA+ E A F + + + +AVFS P VG Sbjct: 297 YSKTNVDSIWAVGDVTDRINLTPVAIREGAAFAQTEFYGNPTTFDHDLVASAVFSQPPVG 356 Query: 354 TVGLSEEQARSEHGEVKIFKTDFKPLKNTLSRNTERTFMKLVVDAKSDRVLGVHMVGGDA 413 VG+SE +AR G+V I+++ F+P+K T ER +KLVV +R+LGVH+VG D+ Sbjct: 357 AVGMSEAEARQAFGKVDIYRSIFRPMKVTFYGGQERCLIKLVVKQDDERILGVHVVGPDS 416 Query: 414 GEVIQGFAVALQCGATKAQFDTTIGIHPTSAEEFVTLR 451 E+IQ A+A++ G TK Q+D+T +HPT AEE VT+R Sbjct: 417 PEIIQMAAIAVKMGVTKPQWDSTCAVHPTLAEELVTMR 454 >all4968 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Nos Nostoc sp. PCC 7120 Length = 459 Score = 402 bits (1033), Expect = e-112, Method: Compositional matrix adjust. Identities = 211/462 (45%), Positives = 295/462 (63%), Gaps = 19/462 (4%) Query: 4 YDYDLFTIGGGSGGVRAARFAGQAGARVGLAEKGELGGTCVNVGCIPKKLMSYSAHYHED 63 +DYDLF IG GSGG+ A++ A GA+V +AE +GGTCV GC+PKKLM Y +H+ Sbjct: 3 FDYDLFVIGAGSGGLAASKRAASYGAKVAIAENDLVGGTCVIRGCVPKKLMVYGSHFPAL 62 Query: 64 FADAAGYGWTLHSQPSFDWVALIANKDREIAHLNDIYQRLLDGANVTLHRGFATVEDAHT 123 F DAAGYGW + + +W I + D+E+ L+ ++ L+ A V L G AT+ D HT Sbjct: 63 FEDAAGYGWQV-GKAELNWEHFITSIDKEVRRLSQLHISFLEKAGVELISGRATLVDNHT 121 Query: 124 VNVDGQRFTARHILVATGGHPDKPQIAGSEWGITSDDFFHLPGLPQRAVVLGGGYIAVEL 183 V V ++FTA IL+A GG P KP++ G E+GITS++ FHL P+ ++G GYI E Sbjct: 122 VEVGERKFTADKILIAVGGRPIKPELPGMEYGITSNEIFHLKTQPKHIAIIGSGYIGTEF 181 Query: 184 ASILNGLGSEVTLVYRRERLLRNMDADLGIHLADEMAKKGIRLIFNASIDAIEAAHAGPD 243 A I+ GLGS+VT + R +++L+ D D+ + + M GIR+I + AIE P+ Sbjct: 182 AGIMRGLGSQVTQITRGDKILKGFDEDIRTEIQEGMTNHGIRIIPKNVVTAIEQV---PE 238 Query: 244 SLKQVHLT--NGETLAAECVLFATGRTPNTRGLGLEQAGVALKENG----------AVLV 291 LK + L+ + E + A+ L ATGR PN GLGLE AGV + ++ A+ V Sbjct: 239 GLK-ISLSGEDQEPIIADVFLVATGRVPNVDGLGLENAGVDVVDSSIEGPGYSTMNAIAV 297 Query: 292 NDNFESSVPSILAVGDVIDRVALTPVALAEAMAVVARLFGQGERSMSYRNIPTAVFSHPN 351 N+ ++S P+I AVGDV DR+ LTPVA+ E A FG R S+ I TAVFS+P Sbjct: 298 NEYSQTSQPNIYAVGDVTDRLNLTPVAIGEGRAFADSEFGNNRREFSHETIATAVFSNPQ 357 Query: 352 VGTVGLSEEQARSEHGE--VKIFKTDFKPLKNTLSRNTERTFMKLVVDAKSDRVLGVHMV 409 TVGL+E +AR++ G+ V I++T F+P+ ++ + ER MKLVVD K+D+VLG HMV Sbjct: 358 ASTVGLTEAEARAKLGDDAVTIYRTRFRPMYHSFTGKQERIMMKLVVDTKTDKVLGAHMV 417 Query: 410 GGDAGEVIQGFAVALQCGATKAQFDTTIGIHPTSAEEFVTLR 451 G +A E+IQG A+A++ GATK FD T+GIHP+SAEEFVT+R Sbjct: 418 GENAAEIIQGVAIAVKMGATKKDFDATVGIHPSSAEEFVTMR 459 >mll0523 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Mlo Mesorhizobium loti Length = 463 Score = 399 bits (1025), Expect = e-111, Method: Compositional matrix adjust. Identities = 211/456 (46%), Positives = 292/456 (64%), Gaps = 7/456 (1%) Query: 1 MSQYDYDLFTIGGGSGGVRAARFAGQAGARVGLAEKGELGGTCVNVGCIPKKLMSYSAHY 60 M+ YDYDLF IGGGSGGVRAAR A G RVG+AE+ GGTCV GC+PKKL Y++ + Sbjct: 1 MAGYDYDLFVIGGGSGGVRAARVAATLGKRVGIAEEYRFGGTCVIRGCVPKKLYVYASQF 60 Query: 61 HEDFADAAGYGWTLHSQPSFDWVALIANKDREIAHLNDIYQRLLDGANVTLHRGFATVED 120 E FADAAGYGWT+ + SF+W L+ANKDREI+ L IY++ ++GA A + D Sbjct: 61 PEHFADAAGYGWTV-PEASFNWQTLVANKDREISRLEAIYKKNVEGAGGETFPSRAMIVD 119 Query: 121 AHTVNV--DGQRFTARHILVATGGHPD-KPQIAGSEWGITSDDFFHLPGLPQRAVVLGGG 177 H V++ D + TA IL+ATGG P P + G E I S++ F L LP+ ++ GGG Sbjct: 120 PHVVHLLDDDRTVTADQILIATGGRPAAHPALPGHEHCIFSNEAFDLKELPKAIMIEGGG 179 Query: 178 YIAVELASILNGLGSEVTLVYRRERLLRNMDADLGIHLADEMAKKGIRLIFNASIDAIEA 237 YIAVE A+I +GLG + TLVYR + +L D DL L + M KKGI+++ +A E Sbjct: 180 YIAVEFANIFHGLGVDTTLVYRGKEILSRFDMDLRRTLHETMEKKGIKILCHA---VSER 236 Query: 238 AHAGPDSLKQVHLTNGETLAAECVLFATGRTPNTRGLGLEQAGVALKENGAVLVNDNFES 297 H PD LT+G+TL + V+ A GR PNT +GLE G+ + + GA+ V++ + Sbjct: 237 VHKRPDGRLDALLTSGQTLTVDQVMLAIGRIPNTENMGLEGIGLEMTQAGAIKVDEYSRT 296 Query: 298 SVPSILAVGDVIDRVALTPVALAEAMAVVARLFGQGERSMSYRNIPTAVFSHPNVGTVGL 357 ++ +I A+GDV +RV LTPVA+ EAM V F + + I TAVFS P +GTVGL Sbjct: 297 NIDNIWAIGDVTNRVQLTPVAIHEAMCFVETAFKGNPTAPDHDTIATAVFSQPEIGTVGL 356 Query: 358 SEEQARSEHGEVKIFKTDFKPLKNTLSRNTERTFMKLVVDAKSDRVLGVHMVGGDAGEVI 417 SE++A +V+I++ F+P+++TLS E+ +KLVVD S +VLG H++G DAGE+ Sbjct: 357 SEDEAVKRFADVEIYRASFRPMRHTLSGRDEKMLIKLVVDGASRKVLGAHILGPDAGEMA 416 Query: 418 QGFAVALQCGATKAQFDTTIGIHPTSAEEFVTLRTP 453 Q + L+ G +K FD T+ +HPT+AEE VT+ P Sbjct: 417 QLLGIPLKAGLSKDDFDRTMAVHPTAAEELVTMYKP 452