BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3236.p (399 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1143 [J] COG0180 Tryptophanyl-tRNA synthetase Rso Ralstonia s... 675 0.0 TM0492 [J] COG0180 Tryptophanyl-tRNA synthetase Tma Thermotoga m... 221 2e-57 DR0558 [J] COG0180 Tryptophanyl-tRNA synthetase Dra Deinococcus ... 204 3e-52 BMEI1806 [J] COG0180 Tryptophanyl-tRNA synthetase Bme Brucella m... 180 6e-45 RC0710 [J] COG0180 Tryptophanyl-tRNA synthetase Rco Rickettsia c... 176 1e-43 >RSc1143 [J] COG0180 Tryptophanyl-tRNA synthetase Rso Ralstonia solanacearum Length = 400 Score = 675 bits (1741), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/399 (78%), Positives = 356/399 (89%) Query: 1 MYPDRVVSGMRPTGALHLGHYHGALKNWVKLQSELPCLFFVADWHALTTHYDDPSVIETS 60 M+P+RV+SGMRPTGALHLGHYHG LKNWV+LQ+E PC FFVADWHALTTHY+ P VIE S Sbjct: 1 MFPERVLSGMRPTGALHLGHYHGVLKNWVRLQAEYPCFFFVADWHALTTHYESPEVIEES 60 Query: 61 TWEMVIDWLAAGVDPSQATLFIQSRVPEHAELHLLLSMATPLGWLERVPTYKDQQEKLAD 120 WEM+IDWLAAGVDP+QATLFIQSRVPEHAEL LLLSM TPLGWLERVPTYKDQ EKL + Sbjct: 61 VWEMLIDWLAAGVDPTQATLFIQSRVPEHAELFLLLSMGTPLGWLERVPTYKDQIEKLKE 120 Query: 121 RDLATYGFLGYPLLQAADVLIYRASQVPVGEDQVPHIEIMREIARRFNHLYGKEKGFEEK 180 +DL+TYGFLGYPLLQAAD+LIYRA VPVGEDQVPH+E+ RE+ARRFN+LYG+E GFE+K Sbjct: 121 KDLSTYGFLGYPLLQAADILIYRAGFVPVGEDQVPHVEMTREVARRFNYLYGREPGFEQK 180 Query: 181 ALAATKKLGSKRAKLYLELRTQFQEKGDDEALEQAKAMLDDAQNLSMIDRERLFGYLEGS 240 AL A KKLG KRAKLYLELRT QE+G+D+ALEQA+A+L ++Q+LSM DRERLFGYLEG+ Sbjct: 181 ALDAAKKLGGKRAKLYLELRTAHQERGEDDALEQARALLAESQSLSMGDRERLFGYLEGA 240 Query: 241 RKLILTEPQAMLTEASRLPGLDGAKMSKSYGNAIALREDAATVTKKVKTMPTDPQRVRRS 300 RK+IL EPQ +LTEASR+PGLDG KMSKSYGN I +RED A+V KKV+TMPTDP RVRR+ Sbjct: 241 RKIILPEPQVLLTEASRMPGLDGQKMSKSYGNTIRMREDKASVEKKVRTMPTDPARVRRT 300 Query: 301 DPGDPDKCPVWQFHQVYSTPEVKEWAAKGCRSAGIGCLECKQPVIDAILQEQQPMLERAQ 360 DPGDP KCPVWQ HQVYS + +EW KGCRSAGIGCLECKQPVID IL+EQQPMLERAQ Sbjct: 301 DPGDPGKCPVWQLHQVYSDADTREWVQKGCRSAGIGCLECKQPVIDGILREQQPMLERAQ 360 Query: 361 PYLDDPSLVRAIVADGCEKARKLAQDTMRDVREAMGLGY 399 Y+DDPSL+RAI+ADGC+ A K+ Q+TMR+VREAMGL Y Sbjct: 361 KYMDDPSLLRAIIADGCDTAHKVTQETMREVREAMGLTY 399 >TM0492 [J] COG0180 Tryptophanyl-tRNA synthetase Tma Thermotoga maritima Length = 328 Score = 221 bits (563), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 113/197 (57%), Positives = 142/197 (72%), Gaps = 5/197 (2%) Query: 5 RVVSGMRPTGALHLGHYHGALKNWVKLQSE-LPCLFFVADWHALTTHYDDPSVIETSTWE 63 R++SGMRPTG LH+GH GAL+NWVKLQ E C +FVADWHALTTHYDD S ++ T + Sbjct: 2 RILSGMRPTGKLHIGHLVGALENWVKLQEEGNECFYFVADWHALTTHYDDVSKLKEYTRD 61 Query: 64 MVIDWLAAGVDPSQATLFIQSRVPEHAELHLLLSMATPLGWLERVPTYKDQQEKLADRDL 123 +V +LA G+DP ++ +F+QS V EHAEL LL SM + LERVPTYK+ + +L +DL Sbjct: 62 LVRGFLACGIDPEKSVIFVQSGVKEHAELALLFSMIVSVSRLERVPTYKEIKSELNYKDL 121 Query: 124 ATYGFLGYPLLQAADVLIYRASQVPVGEDQVPHIEIMREIARRFNHLYGKEKGFEEKALA 183 +T GFL YP+LQAAD+LIY+A VPVGEDQV HIE+ REIARRFN+LY + E L+ Sbjct: 122 STAGFLIYPVLQAADILIYKAEGVPVGEDQVYHIELTREIARRFNYLYDEVFPEPEAILS 181 Query: 184 ATKKL----GSKRAKLY 196 KL G K +K Y Sbjct: 182 RVPKLPGTDGRKMSKSY 198 Score = 145 bits (367), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 69/169 (40%), Positives = 105/169 (62%), Gaps = 4/169 (2%) Query: 232 RLFGYLEGSRKLILTEPQAMLTEASRLPGLDGAKMSKSYGNAIALREDAATVTKKVKTMP 291 R F YL + EP+A+L+ +LPG DG KMSKSYGN I L + + + M Sbjct: 163 RRFNYLYDE---VFPEPEAILSRVPKLPGTDGRKMSKSYGNIINLEISEKELEQTILRMM 219 Query: 292 TDPQRVRRSDPGDPDKCPVWQFHQVYS-TPEVKEWAAKGCRSAGIGCLECKQPVIDAILQ 350 TDP RVRRSDPG+P+ CPVW++HQ + + E +W +GC +A IGC++CK+ ++ + + Sbjct: 220 TDPARVRRSDPGNPENCPVWKYHQAFDISEEESKWVWEGCTTASIGCVDCKKLLLKNMKR 279 Query: 351 EQQPMLERAQPYLDDPSLVRAIVADGCEKARKLAQDTMRDVREAMGLGY 399 + P+ E + +DP V ++ +G +KAR++A TM +VR AM L + Sbjct: 280 KLAPIWENFRKIDEDPHYVDDVIMEGTKKAREVAAKTMEEVRRAMNLMF 328 >DR0558 [J] COG0180 Tryptophanyl-tRNA synthetase Dra Deinococcus radiodurans Length = 330 Score = 204 bits (519), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 132/397 (33%), Positives = 197/397 (49%), Gaps = 76/397 (19%) Query: 5 RVVSGMRPTGALHLGHYHGALKNWVKL--QSELPCLFFVADWHALTT-HYDDPSVIETST 61 RV SG++PTG H+G+Y GA++N+V+L Q L+ V D HA+T DP + T Sbjct: 3 RVFSGIQPTGDPHIGNYFGAMQNYVRLGEQYGKQSLYCVVDLHAITNPGAFDPQTLAQKT 62 Query: 62 WEMVIDWLAAGVDPSQATLFIQSRVPEHAELHLLLSMATPLGWLERVPTYKDQQEKLADR 121 ++M + A G+DPS+ F+QS VPEH EL + + TP+G LER+ YKD K A Sbjct: 63 FDMAVANFAIGLDPSKVVFFVQSHVPEHQELSWIFTCVTPVGELERMTQYKD---KSAQF 119 Query: 122 DLATYGFLGYPLLQAADVLIYRASQVPVGEDQVPHIEIMREIARRFNHLYGKEKGFEEKA 181 + G L YP L AAD+L+Y+A VPVGEDQ HIE+ REIAR+FNH +G Sbjct: 120 ESVPSGLLMYPALMAADILLYKADIVPVGEDQTQHIELTREIARKFNHNFG--------- 170 Query: 182 LAATKKLGSKRAKLYLELRTQFQEKGDDEALEQAKAMLD-DAQNLSMIDRERLFGYLEGS 240 E + KA+ + +A + +D + +G+ Sbjct: 171 ----------------------------ETFTEPKAVYNKEALRIPGVDGQGKMSKSKGN 202 Query: 241 RKLILTEPQAMLTEASRLPGLDGAKMSKSYGNAIALREDAATVTKKVKTMPTDPQRVRRS 300 IL EP + + R+ D A++ +T P DP Sbjct: 203 TIGIL-EPFGDIWQKLRVAPTDPARVR--------------------RTDPGDP------ 235 Query: 301 DPGDPDKCPVWQFHQVYSTPEVKEWAAKGCRSAGIGCLECKQPVIDAILQEQQPMLERAQ 360 DKC + +H+++S PE E +GCR+AGIGC++CK+ ++ I + P+ RA Sbjct: 236 -----DKCLIGDYHKLFSPPETLETVYQGCRTAGIGCVDCKKMLMTHITEHLDPIQTRAA 290 Query: 361 PYLDDPSLVRAIVADGCEKARKLAQDTMRDVREAMGL 397 DP VR + G ++AR +A + M +VR+ +G Sbjct: 291 QLRADPDYVRDALRQGDQEARAIASEVMDEVRQKVGF 327 >BMEI1806 [J] COG0180 Tryptophanyl-tRNA synthetase Bme Brucella melitensis Length = 385 Score = 180 bits (456), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 122/393 (31%), Positives = 196/393 (49%), Gaps = 51/393 (12%) Query: 6 VVSGMRPTGALHLGHYHGALKNWVKLQSELPCLFFVADWHALTTHYDDPSVIETSTWEMV 65 V SG++PTG LHLG+Y GA+K WV++Q C++ V D HALT DP + ST E+ Sbjct: 38 VFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALTVS-PDPVELMQSTREVT 96 Query: 66 IDWLAAGVDPSQATLFIQSRVPEHAELHLLLSMATPLGWLERVPTYKDQQEKLADRDLAT 125 +LAAG+DP ++ +F QSRV +HAEL + + +GW+ R+ +KD+ K DR+ A+ Sbjct: 97 AAFLAAGIDPKKSIVFNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGK--DRENAS 154 Query: 126 YGFLGYPLLQAADVLIYRASQVPVGEDQVPHIEIMREIARRFNHLYGKEKGFEEKALAAT 185 G YP L AAD+L+YRA+ VPVGEDQ H+E+ R+IA++FN+ Y + Sbjct: 155 LGLFAYPSLMAADILLYRATAVPVGEDQKQHLELTRDIAQKFNNDYSDR--IASLGVGVD 212 Query: 186 KKLGSKRAKLYLELRTQFQEKGDDEALEQAKAMLDDAQNLSMIDRERLFGYLEGSRKLIL 245 K+G ++ + L E + AM R+ +G++K+ Sbjct: 213 MKVGDEQVSGFFPLT---------EPMISGPAM-------------RIMSLRDGTKKMSK 250 Query: 246 TEPQAMLTEASRLPGLDGAKMSKSYGNAIALREDAATVTKKVKTMPTDPQRVRRSDPGDP 305 ++P ++ SR I L +D T+TKK++ TD + G Sbjct: 251 SDP----SDLSR----------------INLIDDEDTITKKIRKAKTDSDGLPSEVDGLE 290 Query: 306 DKCPVWQFHQVYS--TPEVKEWAAKGCRSAGIGCLECKQPVIDAILQEQQPMLERAQPYL 363 + +Y+ + KE K G + K + D + P+ + + Sbjct: 291 GRPEADNLVGIYAALSSTTKEDVLK--EFGGRQFSDLKASLADLAVARLSPITHEMRRLV 348 Query: 364 DDPSLVRAIVADGCEKARKLAQDTMRDVREAMG 396 DP+ + +++ DG E+A +A+ TMR VR+ +G Sbjct: 349 ADPAHIDSVLRDGGEQAGAIAEQTMRHVRDIVG 381 >RC0710 [J] COG0180 Tryptophanyl-tRNA synthetase Rco Rickettsia conorii Length = 330 Score = 176 bits (445), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 84/169 (49%), Positives = 116/169 (68%), Gaps = 3/169 (1%) Query: 6 VVSGMRPTGALHLGHYHGALKNWVKLQSELPCLFFVADWHALTTHYDDPSVIETSTWEMV 65 V+SG++ TG+LHLG+Y GA+KNWVK+Q E C FF+AD HA+T PS + S E++ Sbjct: 5 VLSGVQATGSLHLGNYLGAIKNWVKMQEEYNCFFFLADLHAITVDI-KPSELNNSIMEVL 63 Query: 66 IDWLAAGVDPSQATLFIQSRVPEHAELHLLLSMATPLGWLERVPTYKDQQEKLADRDLAT 125 +LAAG++P + T+F QS V EH EL LL+ TPLGWL+R+ +KD+ +D++ A Sbjct: 64 AVYLAAGLNPDKVTIFAQSMVKEHTELAWLLNCVTPLGWLKRMTQFKDKAG--SDQEKAC 121 Query: 126 YGFLGYPLLQAADVLIYRASQVPVGEDQVPHIEIMREIARRFNHLYGKE 174 G YP+L AAD+LIY+A VPVGEDQ H+E+ R+IA N + KE Sbjct: 122 LGLFSYPVLMAADILIYKADIVPVGEDQKQHLELTRDIAGVINRKFNKE 170 Score = 43.1 bits (100), Expect = 0.001, Method: Compositional matrix adjust. Identities = 41/163 (25%), Positives = 74/163 (45%), Gaps = 8/163 (4%) Query: 241 RKLILTEPQAMLTE-ASRLPGL-DG-AKMSKSY---GNAIALREDAATVTKKVKTMPTDP 294 K IL P+ +++E +R+ L DG KMSKS + I L++D + +K+K TD Sbjct: 168 NKEILKVPEVLISETGTRIMSLRDGLKKMSKSDISDFSRINLKDDNDLIHQKIKKAKTDH 227 Query: 295 QRVRRSDPGDPDKCPVWQFHQVYSTPEVKEWAAKGCRSAGIGCLECKQPVIDAILQEQQP 354 D + + +YS+ + G + K+ + + I+ QP Sbjct: 228 LSFVSYDQ--ETRPEISNLLDIYSSLSEESLEQIIGNYQNQGFAKFKEDLAEIIITNLQP 285 Query: 355 MLERAQPYLDDPSLVRAIVADGCEKARKLAQDTMRDVREAMGL 397 + ++ ++D + I+ G EKAR A +T+ +V+E G Sbjct: 286 IRDKYLELMNDKEYLLKILHKGAEKARIRASETVNEVKEQFGF 328