BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3106.p (208 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0418 [E] COG1280 Putative threonine efflux protein Rso Ralsto... 167 1e-41 MA1855 [E] COG1280 Putative threonine efflux protein Mac Methano... 155 7e-38 BH0429 [E] COG1280 Putative threonine efflux protein Bha Bacillu... 152 4e-37 RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralsto... 137 2e-32 BH3495 [E] COG1280 Putative threonine efflux protein Bha Bacillu... 132 5e-31 >RSc0418 [E] COG1280 Putative threonine efflux protein Rso Ralstonia solanacearum Length = 206 Score = 167 bits (423), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 107/208 (51%), Positives = 151/208 (72%), Gaps = 2/208 (0%) Query: 1 MPDLFLFLLAAMTLTLAPGPDNLYVLTRGVAQGRQAGLVAAAGFCSGLIFHTLLAVLGFA 60 MP++ LFLL+++ +T+ PGPDNL V+ RG +QGR+AGL AAAGF SG +FHT LA LG A Sbjct: 1 MPNVLLFLLSSVAITVMPGPDNLQVIARGASQGRKAGLAAAAGFASGCLFHTTLAALGLA 60 Query: 61 ALIKAYAPAYHALQYAGAAYLAYLGVRTLRSASAGIALAADGQGMSAVPLRRIYWQSVLA 120 A++++ A+ A+++ GAAYL +LGV+ LRS G+ + Q +A L +++ QSVLA Sbjct: 61 AVLQSAPAAFQAIRWLGAAYLVWLGVQALRS-KGGVGPSVSAQA-AAPDLWQVFRQSVLA 118 Query: 121 NMLNPKVTLFFIAFLPQFVRREAGHEALQMLVLALLFIVQAFVIFSAIALFSGSVGAYFR 180 NMLNPKVTLFF+ FLPQFV +AGH ALQML+L +F+ Q V+F + ++G + R Sbjct: 119 NMLNPKVTLFFVVFLPQFVDAQAGHAALQMLLLGGVFMAQTIVVFGLYGWCAAALGGWMR 178 Query: 181 RRATASVHLNRLAGCAFIGLGIRMALPE 208 R AS+ L+R++GC FIGLG+R+AL + Sbjct: 179 RTPRASLWLDRVSGCIFIGLGLRVALTK 206 >MA1855 [E] COG1280 Putative threonine efflux protein Mac Methanosarcina acetivorans str.C2A Length = 211 Score = 155 bits (391), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 82/209 (39%), Positives = 131/209 (62%), Gaps = 14/209 (6%) Query: 3 DLFLFLLAAMTLTLAPGPDNLYVLTRGVAQGRQAGLVAAAGFCSGLIFHTLLAVLGFAAL 62 L F+ A+ LT PGPD L+VLT+ ++QG+ AG+ A G C+G++ HT A LG +AL Sbjct: 9 QLIYFIAASAALTFLPGPDILFVLTQSISQGKMAGIATATGLCTGILVHTSAAALGISAL 68 Query: 63 IKAYAPAYHALQYAGAAYLAYLGVRTLRSASAGIALAADGQGMSAVPLRR-----IYWQS 117 + A A+ ++YAGAAYL YL + L+ + G+ +S+ P+R +Y + Sbjct: 69 LYKSALAFEIVKYAGAAYLLYLAWQALKES---------GELVSSAPVRETNALALYRRG 119 Query: 118 VLANMLNPKVTLFFIAFLPQFVRREAGHEALQMLVLALLFIVQAFVIFSAIALFSGSVGA 177 + N+LNPKV LFF+AFLPQFV E+G +QM+ L ++F++QA++IFS+I++F+G +G Sbjct: 120 IFMNVLNPKVALFFLAFLPQFVNLESGSVPIQMIFLGIVFLIQAWLIFSSISIFAGIIGD 179 Query: 178 YFRRRATASVHLNRLAGCAFIGLGIRMAL 206 +R ++N F +G+++AL Sbjct: 180 KIVQRPGIGKYINWGKAGIFTVIGVKLAL 208 >BH0429 [E] COG1280 Putative threonine efflux protein Bha Bacillus halodurans Length = 207 Score = 152 bits (385), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 83/208 (39%), Positives = 129/208 (62%), Gaps = 4/208 (1%) Query: 1 MPDLFLFLLAAMTLTLAPGPDNLYVLTRGVAQGRQAGLVAAAGFCSGLIFHTLLAVLGFA 60 + L FL A+ LTL PGPD L+VL + ++Q RQAG+V A G C+GL+ H AV+G + Sbjct: 3 LTSLLSFLGVAVLLTLMPGPDILFVLAQSMSQNRQAGIVTALGLCTGLLVHITAAVVGIS 62 Query: 61 ALIKAYAPAYHALQYAGAAYLAYLGVRTLRSASAGIALAADGQGMSAVPLRRIYWQSVLA 120 ALI A A+ ++YAGAAYL YL + + G+++ D Q + + +Y + ++ Sbjct: 63 ALIYQSALAFTVVKYAGAAYLLYLAWKAFQEKGEGLSI--DKQ--TTLAYGALYKKGIIM 118 Query: 121 NMLNPKVTLFFIAFLPQFVRREAGHEALQMLVLALLFIVQAFVIFSAIALFSGSVGAYFR 180 N+LNPKV+LFF+A LPQFV AG QML+L ++F++QAF+IFS ++ F+ VG Sbjct: 119 NVLNPKVSLFFLALLPQFVNSGAGSAPWQMLLLGVVFLIQAFIIFSLVSWFAEKVGQLLM 178 Query: 181 RRATASVHLNRLAGCAFIGLGIRMALPE 208 R + ++R+ G +G+++A + Sbjct: 179 RSSFIKNQMHRIKGGLLALIGLQVAFSK 206 >RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralstonia solanacearum Length = 215 Score = 137 bits (344), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 84/204 (41%), Positives = 117/204 (57%), Gaps = 1/204 (0%) Query: 3 DLFLFLLAAMTLTLAPGPDNLYVLTRGVAQGRQAGLVAAAGFCSGLIFHTLLAVLGFAAL 62 +L +F+LA + L + PGPD Y++ R V+QGR AGL+++ G +G H L G AL Sbjct: 8 NLPVFMLAVLLLNVTPGPDTAYIVGRSVSQGRAAGLMSSLGVSAGCCVHVLAVAFGLTAL 67 Query: 63 IKAYAPAYHALQYAGAAYLAYLGVRTLRS-ASAGIALAADGQGMSAVPLRRIYWQSVLAN 121 + A A A+ ++ GAAYL YLG R L + A + A A + PLR ++ Q L N Sbjct: 68 LAASAVAFTVIKAIGAAYLIYLGGRMLLAPAGSDDAPVAQAVATARRPLRALFMQGFLTN 127 Query: 122 MLNPKVTLFFIAFLPQFVRREAGHEALQMLVLALLFIVQAFVIFSAIALFSGSVGAYFRR 181 +LNPKV LFF++F PQFV A H+A LVL +FIV + S +A + SV Sbjct: 128 VLNPKVVLFFLSFFPQFVDPGASHKAAAFLVLGAVFIVMSTAWNSLVAWVAASVTRRVAG 187 Query: 182 RATASVHLNRLAGCAFIGLGIRMA 205 + L+R+ G AFIGLG R+A Sbjct: 188 KPGIKRWLDRVVGTAFIGLGARLA 211 >BH3495 [E] COG1280 Putative threonine efflux protein Bha Bacillus halodurans Length = 208 Score = 132 bits (332), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 68/203 (33%), Positives = 119/203 (58%), Gaps = 4/203 (1%) Query: 6 LFLLAAMTLTLAPGPDNLYVLTRGVAQGRQAGLVAAAGFCSGLIFHTLLAVLGFAALIKA 65 LF+ +A+ L + PGPD ++ +T+G+ GR+AG++ A G G I HTL AVLG + +I+ Sbjct: 9 LFMGSAIMLIIIPGPDLVFTITQGMTNGRKAGVITAMGLSLGNIVHTLAAVLGLSLIIQT 68 Query: 66 YAPAYHALQYAGAAYLAYLGVRTLRSASAGIALAADGQGMSAVPLRRIYWQSVLANMLNP 125 + + AGA YL YL + L+ I + ++ L+ ++ + ++ N+LNP Sbjct: 69 SVVVFTIFKLAGAMYLFYLAYKALKHRKEEIRVNSEKTN----DLKGLFLRGLIMNVLNP 124 Query: 126 KVTLFFIAFLPQFVRREAGHEALQMLVLALLFIVQAFVIFSAIALFSGSVGAYFRRRATA 185 KV +F++ FLPQFV ++ + +LQ+ +L L+FI+ +IFS FSG+ + + Sbjct: 125 KVAIFYLTFLPQFVNYQSANISLQLCILGLIFIIMTAIIFSIFGYFSGTFRDRLLKNSRF 184 Query: 186 SVHLNRLAGCAFIGLGIRMALPE 208 + ++N A FIGLG+++ + Sbjct: 185 NEYMNIAATIIFIGLGLKLMTTQ 207