BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2897.p (259 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2356_1 [H] COG1587 Uroporphyrinogen-III synthase Rso Ralstoni... 172 9e-43 PA5259 [H] COG1587 Uroporphyrinogen-III synthase Pae Pseudomonas... 91 4e-18 STM3937 [H] COG1587 Uroporphyrinogen-III synthase Sty Salmonella... 75 1e-13 hemD [H] COG1587 Uroporphyrinogen-III synthase Eco Escherichia c... 71 2e-12 ZhemD [H] COG1587 Uroporphyrinogen-III synthase EcZ Escherichia ... 71 2e-12 >RSc2356_1 [H] COG1587 Uroporphyrinogen-III synthase Rso Ralstonia solanacearum Length = 368 Score = 172 bits (435), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 109/254 (42%), Positives = 141/254 (55%), Gaps = 6/254 (2%) Query: 7 VVVTRPLQQAQAFAASVEQLGRRAVIFPLLTIGPVADDAELKATLAHLHEFALVVFVSPN 66 V+VTRP QA A++E+ G R + FPLL I P + +L+A LA +ALVVFVSPN Sbjct: 24 VIVTRPRAQAPMLVAALERHGLRTLQFPLLDIAPTPNLDDLRAALADPSRYALVVFVSPN 83 Query: 67 AIDAAFAHLSG---WPSQVPIGIVGEGSRLALRAHGVDNSNATLYGP-QRAD--KMDSEE 120 A+ AFA + WP VP +VG S AL AHGV + P AD + DSE Sbjct: 84 AVQQAFAAMPEGFRWPQDVPAAVVGPASAQALAAHGVAAPTYRVIRPDSHADDARQDSEA 143 Query: 121 LLKSLPMEQLRNKRALIVRGQTGRDFLSEALQQHGVTVEHLTAYRRLAPTLDARSGALLL 180 L L + L + LIVRG GR++L++ L++ GV V + AYRR P D + A L Sbjct: 144 LYARLDVATLSGREVLIVRGNGGREWLADRLREAGVAVRTVEAYRRSVPVPDTAAWAALR 203 Query: 181 DLLDGGADWVITSSEALRNLLDLARLAGGEERVVKLQRQRIVVSHHRIAETARQQGFVQV 240 ++L G W +TSSEA RNL LAR + V L H RI E A+ GF V Sbjct: 204 EVLAGRHAWTLTSSEAARNLDSLARASLSAAEVEALYAAPCFAPHARIVEQAQSLGFRDV 263 Query: 241 ILTGSGDERLLAAL 254 LTG+GD+RLLA + Sbjct: 264 TLTGAGDDRLLAGV 277 >PA5259 [H] COG1587 Uroporphyrinogen-III synthase Pae Pseudomonas aeruginosa Length = 251 Score = 90.5 bits (223), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 82/246 (33%), Positives = 125/246 (50%), Gaps = 29/246 (11%) Query: 7 VVVTRPLQQAQAFAASVEQLGRRAVIFPLLTIGPVADDAELKATLAHLHEFALVVFVSPN 66 +++TRP ++ A AAS+ + G + PLL I P+ + E + + L + VV VS Sbjct: 6 LLLTRPDEECAALAASLGEAGVHSSSLPLLAIDPLEETPEQRTLMLDLDRYCAVVVVSKP 65 Query: 67 AIDAAFAHLSGW---PSQVPIGIVGEGSRLALRAHGVDNSNATLYGPQRADKMDSEELLK 123 A L + P Q VG + L A+G+D + P++ D DSE LL Sbjct: 66 AARLGLERLDRYWPQPPQQTWCSVGAATAAILEAYGLDVTY-----PEQGD--DSEALL- 117 Query: 124 SLPMEQ----LRNKRALIVRGQTGRDFLSEALQQHGVTVEHLTAYRRLAPTLDARSGALL 179 +LP Q + + + LI+RG+ GR+FL+E L+ GV V++L YRR AP D +G LL Sbjct: 118 ALPAFQDSLRVHDPKVLIMRGEGGREFLAERLRGQGVQVDYLPLYRRRAP--DYPAGELL 175 Query: 180 ----LDLLDGGADWVITSSEALRNLLDLARLAGGEERVVKLQRQRIVVSHHRIAETARQQ 235 + L+G V++S + L+NL LA E + R + V R+AE AR+ Sbjct: 176 ARVRAERLNG---LVVSSGQGLQNLYQLAAADWPE-----IGRLPLFVPSPRVAEMAREL 227 Query: 236 GFVQVI 241 G +VI Sbjct: 228 GAQRVI 233 >STM3937 [H] COG1587 Uroporphyrinogen-III synthase Sty Salmonella typhimurium LT2 Length = 246 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 74/247 (29%), Positives = 111/247 (44%), Gaps = 14/247 (5%) Query: 5 LPVVVTRPLQQAQAFAASVEQLGRRAVIFPLLTIGPVADDAELKATLAHLHEFALVVFVS 64 + +++TRP +A + + LG+ A FPL+ + L LA L E LV +S Sbjct: 1 MSILITRPSPAGEALVSRLRALGQVAWSFPLIEFVAGRELPTLADRLATLTENDLVFALS 60 Query: 65 PNAIDAAFAHLS----GWPSQVPIGIVGEGSRLALRAHGVDNSNATLYGPQRADKMDSEE 120 +A+ A A L WP +G + LAL H V S + P D+ SE Sbjct: 61 QHAVAFAHAQLQRDGRNWPVAPRYFAIGRTTALAL--HTV--SGFDIRYP--LDREISEA 114 Query: 121 LLKSLPMEQLRNKRALIVRGQTGRDFLSEALQQHGVTVEHLTAYRRLAPTLDARSGALLL 180 LL+ ++ + KRALI+RG GR+ L E L G V Y+R A D A+ Sbjct: 115 LLQLPELQNIAGKRALILRGNGGRELLGETLTARGAEVSFCECYQRCAKHYDGAEEAMRW 174 Query: 181 DLLDGGADWVITSSEALRNLLDLARLAGGEERVVKLQRQRIVVSHHRIAETARQQGFVQV 240 G V+TS E L+ L L E ++ R R++V R+A AR+ G+ + Sbjct: 175 H-TRGVTTLVVTSGEMLQRLWSLTPQWYREHWLL---RCRLLVVSERLAHLARELGWQDI 230 Query: 241 ILTGSGD 247 + + D Sbjct: 231 KVADNAD 237 >hemD [H] COG1587 Uroporphyrinogen-III synthase Eco Escherichia coli K12 Length = 246 Score = 71.2 bits (173), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 74/255 (29%), Positives = 117/255 (45%), Gaps = 15/255 (5%) Query: 5 LPVVVTRPLQQAQAFAASVEQLGRRAVIFPLLTIGPVADDAELKATLAHLHEFALVVFVS 64 + ++VTRP + + + LG+ A FPL+ P +L LA L E L+ +S Sbjct: 1 MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLADQLAALGESDLLFALS 60 Query: 65 PNAIDAAFAHLS----GWPSQVPIGIVGEGSRLALRAHGVDNSNATLYGPQRADKMDSEE 120 +A+ A + L WP +G + LAL H V S + PQ D+ SE Sbjct: 61 QHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALAL--HTV--SGQKILYPQ--DREISEV 114 Query: 121 LLKSLPMEQLRNKRALIVRGQTGRDFLSEALQQHGVTVEHLTAYRRLAPTLDARSGALLL 180 LL+ ++ + KRALI+RG GR+ + + L G V Y+R A D A+ Sbjct: 115 LLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQRCAIHYDGAEEAMRW 174 Query: 181 DLLDGGADWVITSSEALRNLLDLARLAGGEERVVKLQRQRIVVSHHRIAETARQQGFVQV 240 + V+TS E L+ L L E ++ R++V R+A+ AR+ G+ + Sbjct: 175 QAREVTMV-VVTSGEMLQQLWSLIPQWYREHWLLHC---RLLVVSERLAKLARELGWQDI 230 Query: 241 ILTGSGD-ERLLAAL 254 + + D + LL AL Sbjct: 231 KVADNADNDALLRAL 245 >ZhemD [H] COG1587 Uroporphyrinogen-III synthase EcZ Escherichia coli O157:H7 EDL933 Length = 246 Score = 70.9 bits (172), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 74/255 (29%), Positives = 117/255 (45%), Gaps = 15/255 (5%) Query: 5 LPVVVTRPLQQAQAFAASVEQLGRRAVIFPLLTIGPVADDAELKATLAHLHEFALVVFVS 64 + ++VTRP + + + LG+ A FPL+ P +L LA L E L+ +S Sbjct: 1 MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLADQLAALGESDLLFALS 60 Query: 65 PNAIDAAFAHLS----GWPSQVPIGIVGEGSRLALRAHGVDNSNATLYGPQRADKMDSEE 120 +A+ A + L WP +G + LAL H V S + PQ D+ SE Sbjct: 61 QHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALAL--HTV--SGQKILYPQ--DREISEV 114 Query: 121 LLKSLPMEQLRNKRALIVRGQTGRDFLSEALQQHGVTVEHLTAYRRLAPTLDARSGALLL 180 LL+ ++ + KRALI+RG GR+ + + L G V Y+R A D A+ Sbjct: 115 LLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQRCAIHYDGAEEAMRW 174 Query: 181 DLLDGGADWVITSSEALRNLLDLARLAGGEERVVKLQRQRIVVSHHRIAETARQQGFVQV 240 + V+TS E L+ L L E ++ R++V R+A+ AR+ G+ + Sbjct: 175 QSREVTTV-VVTSGEMLQQLWSLIPQWYREHWLLHC---RLLVVSERLAKLARELGWQDI 230 Query: 241 ILTGSGD-ERLLAAL 254 + + D + LL AL Sbjct: 231 KVADNADNDALLRAL 245