BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2745.p (408 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1293 [V] COG1566 Multidrug resistance efflux pump Rso Ralston... 439 e-123 PA5159 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomo... 394 e-109 YPO3267 [V] COG1566 Multidrug resistance efflux pump Ype Yersini... 354 2e-97 STM2814 [V] COG1566 Multidrug resistance efflux pump Sty Salmone... 345 1e-94 emrA [V] COG1566 Multidrug resistance efflux pump Eco Escherichi... 340 4e-93 >RSc1293 [V] COG1566 Multidrug resistance efflux pump Rso Ralstonia solanacearum Length = 428 Score = 439 bits (1130), Expect = e-123, Method: Compositional matrix adjust. Identities = 221/376 (58%), Positives = 291/376 (77%), Gaps = 3/376 (0%) Query: 15 NGNGNGKRTRQLILLTVVLVIVAAACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTV 74 NG+GNGKR R L L LV+ LYW L+ R+FE TDDAYV GNVVQ++ QV GTV Sbjct: 24 NGSGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWFESTDDAYVQGNVVQVTPQVAGTV 83 Query: 75 VAVKADDTQLVKLGQPLVALDAADTKLALDQAQAALAQAVRQTRQLFLNNDTLAANVAAA 134 VA++ADDTQLV GQPL+ LD AD ++AL+QA+AALAQAVRQ R L+ N + +A Sbjct: 84 VAIRADDTQLVTAGQPLIELDRADARVALEQAEAALAQAVRQVRTLYSNTGAYTSTLAMR 143 Query: 135 DANLARAREDLQRRQAGLSSGAVSQEDVSHARDALKSATAALDQARAAAAANRALTDHTS 194 +++LA+A+EDL RR+ +GAVSQE+++HA+ +L++A AA++ AR ANR LT+ T+ Sbjct: 144 ESDLAKAKEDLARRKQIAGTGAVSQEEIAHAQTSLQAAEAAVETAREQLQANRVLTEQTT 203 Query: 195 VTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQI 254 + HPNVLQAA VR AYL YAR ++ A V+G+V+KRSVQVGQR+A G PLMAIVPL+Q+ Sbjct: 204 LERHPNVLQAAAKVREAYLAYARTSLPASVTGYVAKRSVQVGQRVAPGTPLMAIVPLDQL 263 Query: 255 WIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNW 314 W+DANFKE Q++H+R+GQPVE++ADVYGSSV Y G V+GFSAGTG AFSLLPAQNATGNW Sbjct: 264 WVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKVVGFSAGTGSAFSLLPAQNATGNW 323 Query: 315 IKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGD-GRTLEAVPTN--YQTNVYNDL 371 IKVVQR+PVR++LDP++++ HPLR+GLS A VDIH + G++L + P QT+VY+ Sbjct: 324 IKVVQRLPVRVSLDPKELKDHPLRVGLSMEARVDIHDEGGQSLASTPATPPLQTSVYDQA 383 Query: 372 DKQADAIVERIISDNA 387 ++AD ++ II+ NA Sbjct: 384 GQEADQVIASIIATNA 399 >PA5159 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomonas aeruginosa Length = 394 Score = 394 bits (1011), Expect = e-109, Method: Compositional matrix adjust. Identities = 200/373 (53%), Positives = 257/373 (68%), Gaps = 4/373 (1%) Query: 18 GNGKRTRQLILLTVVLVIVAAACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVVAV 77 GN KR R L++L ++V+ A + F + R+ E+TDDAY+ GNVVQI+ Q+ GTVV++ Sbjct: 11 GNPKRKRWLLILLAMIVLATLASVAWEFFYGRWHEDTDDAYINGNVVQITPQIVGTVVSI 70 Query: 78 KADDTQLVKLGQPLVALDAADTKLALDQAQAALAQAVRQTRQLFLNNDTLAANVAAADAN 137 ADD LV+ GQ LV D +D +AL +A+A LA VRQ R LF N D A VA Sbjct: 71 GADDGDLVRKGQELVRFDPSDADIALQRAEANLAHTVRQVRGLFSNVDGYRAEVATRKVA 130 Query: 138 LARAREDLQRRQAGLSSGAVSQEDVSHARDALKSATAALDQARAAAAANRALTDHTSVTE 197 LA+A D +RR+ GA+SQE+++HARDAL SA A+L + NRAL D T +T Sbjct: 131 LAKAEADYKRRKNLADDGAISQEELAHARDALDSAKASLTSSEQQLNTNRALVDDTQITS 190 Query: 198 HPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWID 257 HP+V AA +R AYL+ AR IVAPV+G+V+KRSVQVGQR+ GN LMA+VPL+QIWID Sbjct: 191 HPDVKAAAAQLRQAYLDDARSTIVAPVTGYVAKRSVQVGQRVQPGNALMAVVPLDQIWID 250 Query: 258 ANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNWIKV 317 ANFKE+QL+H+RIGQPVE+ +D+YGS V+Y GTV GTG AFSLLPAQNATGNWIK+ Sbjct: 251 ANFKETQLKHMRIGQPVEIRSDLYGSDVRYSGTVDSLGVGTGSAFSLLPAQNATGNWIKI 310 Query: 318 VQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHG-DGRTLEAVPTN---YQTNVYNDLDK 373 VQRVPVRI +DP++++ HPLRIGLS VD+H G L P + T+VY Sbjct: 311 VQRVPVRIHIDPQELQKHPLRIGLSMDVKVDLHDQSGPALAQQPPREALFSTDVYQQQLA 370 Query: 374 QADAIVERIISDN 386 AD ++ER+I N Sbjct: 371 SADKLIERLIEAN 383 >YPO3267 [V] COG1566 Multidrug resistance efflux pump Ype Yersinia pestis Length = 390 Score = 354 bits (908), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 172/341 (50%), Positives = 239/341 (70%), Gaps = 1/341 (0%) Query: 17 NGNGKRTRQLILLTVVLVIVAAACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVVA 76 N +R R L+LLT + +++ A +YWFL R +ETD+AY+ GN VQI +QV G+VV+ Sbjct: 15 NKKQQRKRVLLLLTAIFIMIGVAYLIYWFLVLRHHQETDNAYISGNQVQIMSQVPGSVVS 74 Query: 77 VKADDTQLVKLGQPLVALDAADTKLALDQAQAALAQAVRQTRQLFLNNDTLAANVAAADA 136 V ++T VK G LV LD D + A +QA+ ALA +VRQT QL +N+ AN+A Sbjct: 75 VHFENTDFVKSGDVLVTLDPTDAEQAFEQAKTALANSVRQTHQLIINSKQYQANIALKKT 134 Query: 137 NLARAREDLQRRQAGLSSGAVSQEDVSHARDALKSATAALDQARAAAAANRALTDHTSVT 196 L++A+ DL+RR ++ + +E++ HARDA+++A A+LD A AN+AL +T + Sbjct: 135 ELSQAQNDLKRRVVLGAAAVIGREELQHARDAVEAAQASLDMAVQQYNANQALVLNTPLE 194 Query: 197 EHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWI 256 + P + QAA +R+A+L R +V+P+SG+VS+RSVQVG I++G PLMA+VP +Q+WI Sbjct: 195 KQPAIEQAAAKMRDAWLTLQRTKVVSPISGYVSRRSVQVGAEISSGTPLMAVVPADQLWI 254 Query: 257 DANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNWIK 316 DANFKE+QL ++RIGQP ++ D YG V Y+G V+G GTG AFSLLPAQNATGNWIK Sbjct: 255 DANFKETQLANMRIGQPATIVTDFYGDDVVYQGKVVGLDMGTGSAFSLLPAQNATGNWIK 314 Query: 317 VVQRVPVRIALDPEQVRAHPLRIGLSTTATVDI-HGDGRTL 356 VVQR+PVRIALD +Q++ HPLRIGLS+ VD + DG L Sbjct: 315 VVQRLPVRIALDEKQLKEHPLRIGLSSLVKVDTANTDGHVL 355 >STM2814 [V] COG1566 Multidrug resistance efflux pump Sty Salmonella typhimurium LT2 Length = 390 Score = 345 bits (885), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 186/347 (53%), Positives = 238/347 (68%), Gaps = 5/347 (1%) Query: 11 PAKANGNGNGKRTRQLILLTVVLVIVAAACFLYWFLHARFFEETDDAYVGGNVVQISAQV 70 PAK G KR L+LLT++ VI+A A +YWFL R EETDDAYV GN VQI AQV Sbjct: 13 PAKKKG----KRKTALLLLTLLFVIIAVAYGIYWFLVLRHIEETDDAYVAGNQVQIMAQV 68 Query: 71 GGTVVAVKADDTQLVKLGQPLVALDAADTKLALDQAQAALAQAVRQTRQLFLNNDTLAAN 130 G+V V AD+T VK G LV LD D K A ++A+ ALA +VRQT QL +N+ L AN Sbjct: 69 SGSVTKVWADNTDFVKEGDVLVTLDQTDAKQAFEKAKTALASSVRQTHQLMINSKQLQAN 128 Query: 131 VAAADANLARAREDLQRRQAGLSSGAVSQEDVSHARDALKSATAALDQARAAAAANRALT 190 + LA+A+ DL RR ++ + +E++ HARDA+ SA A LD A AN+A+ Sbjct: 129 IDVQKTALAQAQSDLNRRVPLGNANLIGREELQHARDAVASAQAQLDVAIQQYNANQAMI 188 Query: 191 DHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVP 250 ++++ + P V QAAT VRNA+L R IV+P++G+VS+R+VQ G +I+ PLMA+VP Sbjct: 189 LNSNLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQISPTTPLMAVVP 248 Query: 251 LEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNA 310 +W+DANFKE+QL ++RIGQPV VI D+YG VKY G V+G GTG AFSLLPAQNA Sbjct: 249 ATDLWVDANFKETQLANMRIGQPVTVITDIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNA 308 Query: 311 TGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDI-HGDGRTL 356 TGNWIKVVQR+PVR+ LD Q+ HPLRIGLST TVD + DG+ L Sbjct: 309 TGNWIKVVQRLPVRVELDARQLEQHPLRIGLSTLVTVDTANRDGQVL 355 >emrA [V] COG1566 Multidrug resistance efflux pump Eco Escherichia coli K12 Length = 390 Score = 340 bits (871), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 175/339 (51%), Positives = 235/339 (69%), Gaps = 1/339 (0%) Query: 19 NGKRTRQLILLTVVLVIVAAACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVVAVK 78 +GKR R L+LLT++ +I+A A +YWFL R FEETDDAYV GN +QI +QV G+V V Sbjct: 17 SGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGNQIQIMSQVSGSVTKVW 76 Query: 79 ADDTQLVKLGQPLVALDAADTKLALDQAQAALAQAVRQTRQLFLNNDTLAANVAAADANL 138 AD+T VK G LV LD D + A ++A+ ALA +VRQT QL +N+ L AN+ L Sbjct: 77 ADNTDFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQLMINSKQLQANIEVQKIAL 136 Query: 139 ARAREDLQRRQAGLSSGAVSQEDVSHARDALKSATAALDQARAAAAANRALTDHTSVTEH 198 A+A+ D RR ++ + +E++ HARDA+ SA A LD A AN+A+ T + + Sbjct: 137 AKAQSDYNRRVPLGNANLIGREELQHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQ 196 Query: 199 PNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDA 258 P V QAAT VRNA+L R I++P++G+VS+R+VQ G +I+ PLMA+VP +W+DA Sbjct: 197 PAVQQAATEVRNAWLALERTRIISPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDA 256 Query: 259 NFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNWIKVV 318 NFKE+Q+ ++RIGQPV + D+YG VKY G V+G GTG AFSLLPAQNATGNWIKVV Sbjct: 257 NFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVV 316 Query: 319 QRVPVRIALDPEQVRAHPLRIGLSTTATVD-IHGDGRTL 356 QR+PVRI LD +Q+ +PLRIGLST +V+ + DG+ L Sbjct: 317 QRLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVL 355