BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2704.p (244 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc1613 [S] COG5446 Predicted integral membrane protein Atu Agro... 185 7e-47 PA2946 [S] COG5446 Predicted integral membrane protein Pae Pseud... 179 4e-45 mll1309 Non COG 165 7e-41 BMEI0692 [S] COG5446 Predicted integral membrane protein Bme Bru... 84 3e-16 PA4852 [J] COG0042 tRNA-dihydrouridine synthase Pae Pseudomonas ... 33 0.65 >AGc1613 [S] COG5446 Predicted integral membrane protein Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 241 Score = 185 bits (470), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 102/241 (42%), Positives = 141/241 (58%), Gaps = 10/241 (4%) Query: 4 TVFRRMASAVVIAGALSGVLLSGVQQFQVEKIIFKAEEYEKAGEAAAAQAQEHDHDHDQA 63 + FR + ++ G L G +S +Q F +I +AE +E AGEA H HD Sbjct: 7 SFFRNIVFTAILVGILGGFAVSAMQAFGTTPLILQAETFESAGEA--------PHSHDAP 58 Query: 64 TATSAHAHEHAHDADAWEPADGAERTGYTVLANVSMAVGFALMLVAAFALSGRTISWRSG 123 +A H H+ +AW PADG ERT YTV ANV A+G+AL+L +L G WR G Sbjct: 59 APGAAVP-AHEHNDEAWAPADGFERTAYTVAANVLTAIGYALVLTGLISLRGTNTGWREG 117 Query: 124 LLWGLAGYGVFFVAPSLGLPPEVPGTLAAPLHARQLWWVMTAAMTAASLGLFVFTRGWPY 183 LLWG+AG+ +AP +GLPPE+PG+ AA L ARQ+WW+ T A TA + L F R Sbjct: 118 LLWGVAGFAAVMLAPMIGLPPELPGSPAAALEARQIWWLATVAATAGGIALIAFRREPWA 177 Query: 184 KLVGVLLLVVPHLVGAP-QPEVHGSVAPEELAQAFIHATALANAVFWLALGGLTGFFYRR 242 L+ ++L+V PH++GAP PE ++AP L + FI A + + VFW +G L+ FF +R Sbjct: 178 VLLALVLIVAPHVIGAPLPPEGEHALAPLPLERQFIVAATITSFVFWALIGTLSAFFLKR 237 Query: 243 F 243 F Sbjct: 238 F 238 >PA2946 [S] COG5446 Predicted integral membrane protein Pae Pseudomonas aeruginosa Length = 364 Score = 179 bits (454), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 115/221 (52%), Positives = 142/221 (64%), Gaps = 12/221 (5%) Query: 5 VFRRMASAVVIAGALSGVLLSGVQQFQVEKIIFKAEEYEKAGEAAAAQAQEHDHDHDQAT 64 + +R+A AG L+ +LL+ +Q V +I +AE YEK+ E AAQ EH A Sbjct: 132 MIKRIAQTAGFAGLLAALLLTLLQILWVTPLILEAETYEKS-EPVAAQPHEH------AP 184 Query: 65 ATSAHAHEHAHDADAWEPADGAERTGYTVLANVSMAVGFALMLVAAFALSGRTISWRSGL 124 +AH H D +AWEP DG +RT T N+ +AVGFALML F L +W+ GL Sbjct: 185 GVAAHVH----DEEAWEPEDGWQRTLSTTGGNLVVAVGFALMLAGLFTLRAPGQTWQ-GL 239 Query: 125 LWGLAGYGVFFVAPSLGLPPEVPGTLAAPLHARQLWWVMTAAMTAASLGLFVFTRGWPYK 184 LWGLAGY VF +APSLGLPPE+PGT AA L RQ WW+ TAA TA L L VF WP K Sbjct: 240 LWGLAGYAVFCLAPSLGLPPELPGTAAADLVQRQYWWIATAAATAVGLALLVFGGNWPLK 299 Query: 185 LVGVLLLVVPHLVGAPQPEVHGSVAPEELAQAFIHATALAN 225 LVG +LL +PHL GAPQPEVH S+APE LAQ F+ A+ ++N Sbjct: 300 LVGAVLLALPHLFGAPQPEVHASLAPEALAQRFVVASLVSN 340 >mll1309 Non COG Length = 271 Score = 165 bits (418), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 97/251 (38%), Positives = 130/251 (51%), Gaps = 32/251 (12%) Query: 4 TVFRRMASAVVIAGALSGVLLSGVQQFQVEKIIFKAEEYEKAGEAAAAQAQEHDHDHD-- 61 +FR + IAG ++GV+L+ +Q + +I KAE YE+A H HDH Sbjct: 2 NLFRNVVFIAAIAGLVAGVVLACMQAYATVPLILKAEVYEQAEGG-------HSHDHAAA 54 Query: 62 --------QATATSA---------------HAHEHAHDADAWEPADGAERTGYTVLANVS 98 TA S+ + + W PADG ER + V++NV Sbjct: 55 PATPTGAASGTAMSSAPPAANSMSAATPAPAVAAAPAEDEGWAPADGFERFAFNVVSNVV 114 Query: 99 MAVGFALMLVAAFALSGRTISWRSGLLWGLAGYGVFFVAPSLGLPPEVPGTLAAPLHARQ 158 +GFAL+LVA +G +WR G+ WGLAG+ VF +AP LGLPPE+P AA L RQ Sbjct: 115 TGIGFALILVAVSEFAGGIGNWRQGVFWGLAGFAVFTLAPGLGLPPELPAMPAADLTQRQ 174 Query: 159 LWWVMTAAMTAASLGLFVFTRGWPYKLVGVLLLVVPHLVGAPQPEVHGSVAPEELAQAFI 218 +WWV T TA LGL F + P ++ V L+V PH+VGAPQP+ + PE L F+ Sbjct: 175 IWWVATVIATAIGLGLIAFRKSLPLAILAVALIVAPHIVGAPQPDSFETPIPEGLHHQFV 234 Query: 219 HATALANAVFW 229 A L N VFW Sbjct: 235 VAVTLTNLVFW 245 >BMEI0692 [S] COG5446 Predicted integral membrane protein Bme Brucella melitensis Length = 131 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 46/123 (37%), Positives = 67/123 (54%) Query: 121 RSGLLWGLAGYGVFFVAPSLGLPPEVPGTLAAPLHARQLWWVMTAAMTAASLGLFVFTRG 180 ++G+ WGLAG+ P+LGL PE+P AA L RQLWW+ T M+ + L + Sbjct: 2 QNGIFWGLAGFFAVAFLPALGLSPELPAMPAADLAERQLWWIATVVMSGLGIYLLILRHE 61 Query: 181 WPYKLVGVLLLVVPHLVGAPQPEVHGSVAPEELAQAFIHATALANAVFWLALGGLTGFFY 240 K++G++L+V PHL GAP PE S P LA + A+ N W +G G+F Sbjct: 62 LWAKVLGLVLIVAPHLYGAPHPEDISSPVPSLLASQYAVASLATNLFMWAVIGLALGWFI 121 Query: 241 RRF 243 + + Sbjct: 122 QHY 124 >PA4852 [J] COG0042 tRNA-dihydrouridine synthase Pae Pseudomonas aeruginosa Length = 332 Score = 33.1 bits (74), Expect = 0.65, Method: Compositional matrix adjust. Identities = 34/123 (27%), Positives = 54/123 (43%), Gaps = 12/123 (9%) Query: 84 DGAERTGYTVLANVSMAVGFALMLVAAFALSGRTISWRSGLLWGLAGY-GVFFVAPSLGL 142 D R G TV A ++ G + A A+ GRT R+ L G A Y + + ++ + Sbjct: 148 DRDNRNGVTV-AKLAEQAG-----IQALAVHGRT---RADLYTGEAEYETIAAIKQAVSI 198 Query: 143 PPEVPGTLAAPLHARQLWWVMTAAMTAASLGLFVFTRGWPYKLVGVLLLVVPHLVGAPQP 202 P G + +P AR++ + + A +G R W ++ + L HL AP P Sbjct: 199 PVFANGDIDSPEKARKV--IEQTGVDALLIGRAAQGRPWIFREIDHYLRTGEHLPAAPLP 256 Query: 203 EVH 205 EV Sbjct: 257 EVQ 259