BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2690.p (223 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0614 [C] COG0778 Nitroreductase Rso Ralstonia solanacearum 313 2e-85 PA1274 [C] COG0778 Nitroreductase Pae Pseudomonas aeruginosa 281 6e-76 Rv0306 [C] COG0778 Nitroreductase Mtu Mycobacterium tuberculosis... 248 6e-66 MT0319 [C] COG0778 Nitroreductase MtC Mycobacterium tuberculosis... 248 6e-66 SMc00166 [C] COG0778 Nitroreductase Sme Sinorhizobium meliloti 167 2e-41 >RSp0614 [C] COG0778 Nitroreductase Rso Ralstonia solanacearum Length = 216 Score = 313 bits (802), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 153/211 (72%), Positives = 187/211 (88%) Query: 5 ACHRYPQEQIEALYRVIRERRDMRHFLPDPVDPALLERLLQAAHDGPSVGFMQPWRFIRI 64 HRY + + A+YRVIRERRDMRHF PDP++PALL RLL+AAH PSVG+MQPWRFIRI Sbjct: 3 TTHRYTEADVAAVYRVIRERRDMRHFRPDPIEPALLARLLRAAHLAPSVGYMQPWRFIRI 62 Query: 65 ASRALRQQIHALVEQERIATAQALGQREDEFMRLKVEGILECGELLVAALMDRREAYVFG 124 ALR +IHALVE+ER++TA+ALGQREDEF+RLKVEGIL+CGE+LVAALMD+RE ++FG Sbjct: 63 TDAALRARIHALVEEERLSTARALGQREDEFLRLKVEGILDCGEVLVAALMDKREPHIFG 122 Query: 125 RRTLPQMDLASLACALQNMWLAARAEGLGMGWVSLFEPQALSHLLHLPSGAEPVAVLCLG 184 RRTLP+MDLAS+ACA+QN+WLAARAEG+G+GWVS+F+PQ L LLHLP+GA PVAVLCLG Sbjct: 123 RRTLPEMDLASVACAIQNLWLAARAEGIGVGWVSMFDPQQLGQLLHLPAGARPVAVLCLG 182 Query: 185 HVPAFYDKPMLEQQQWAQRQRLQDLVFQDRW 215 HV AFYD+PMLEQ+ WA RQ L+ ++F++RW Sbjct: 183 HVDAFYDRPMLEQEHWASRQPLESMLFENRW 213 >PA1274 [C] COG0778 Nitroreductase Pae Pseudomonas aeruginosa Length = 218 Score = 281 bits (720), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 140/211 (66%), Positives = 164/211 (77%) Query: 5 ACHRYPQEQIEALYRVIRERRDMRHFLPDPVDPALLERLLQAAHDGPSVGFMQPWRFIRI 64 + H + + A+YR I ERRDMRHF V P LL RLL+AAH PSVG MQPWRFIRI Sbjct: 4 SSHAFSATERAAVYRAIAERRDMRHFSGGSVAPELLARLLEAAHQAPSVGLMQPWRFIRI 63 Query: 65 ASRALRQQIHALVEQERIATAQALGQREDEFMRLKVEGILECGELLVAALMDRREAYVFG 124 ALR+ IH LVE+ER TA+ALG+R DEFMRLKVEG+L+C ELLVAALMD RE +VFG Sbjct: 64 TEPALRRAIHGLVEEERGRTAEALGERSDEFMRLKVEGVLDCAELLVAALMDDRERHVFG 123 Query: 125 RRTLPQMDLASLACALQNMWLAARAEGLGMGWVSLFEPQALSHLLHLPSGAEPVAVLCLG 184 RRTLPQMDLASLACA+QN+WLA+RAEGLGMGWVSLF+P AL LL LP G+E VAVLCLG Sbjct: 124 RRTLPQMDLASLACAIQNLWLASRAEGLGMGWVSLFDPAALGELLGLPPGSEAVAVLCLG 183 Query: 185 HVPAFYDKPMLEQQQWAQRQRLQDLVFQDRW 215 V FY PML + W++ + L +LV+Q+RW Sbjct: 184 PVAEFYPAPMLALEGWSEPRPLAELVYQNRW 214 >Rv0306 [C] COG0778 Nitroreductase Mtu Mycobacterium tuberculosis H37Rv Length = 223 Score = 248 bits (634), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 130/201 (64%), Positives = 159/201 (79%), Gaps = 1/201 (0%) Query: 16 ALYRVIRERRDMRHFLPDPV-DPALLERLLQAAHDGPSVGFMQPWRFIRIASRALRQQIH 74 A+YRVI ERRDMR F+P V +L RLL AAH PSVG MQPWRFIRI L+++IH Sbjct: 9 AVYRVIAERRDMRRFVPGGVVSEDVLARLLHAAHAAPSVGLMQPWRFIRITDETLKRRIH 68 Query: 75 ALVEQERIATAQALGQREDEFMRLKVEGILECGELLVAALMDRREAYVFGRRTLPQMDLA 134 ALV+ ER+ TA+ALG RE+EF+ LKVEGIL+C ELLV AL DRR +Y+FGRRTLPQMDLA Sbjct: 69 ALVDDERLLTAEALGAREEEFLALKVEGILDCAELLVVALCDRRGSYIFGRRTLPQMDLA 128 Query: 135 SLACALQNMWLAARAEGLGMGWVSLFEPQALSHLLHLPSGAEPVAVLCLGHVPAFYDKPM 194 S++CA+QN+WLAAR+EGLGMGWVSLF+PQ L+ LL +P+ AEPVA+LCLG VP F D+P Sbjct: 129 SVSCAIQNLWLAARSEGLGMGWVSLFDPQRLAALLAMPADAEPVAILCLGPVPEFPDRPA 188 Query: 195 LEQQQWAQRQRLQDLVFQDRW 215 LE WA + L + V ++RW Sbjct: 189 LELDGWAYARPLAEFVSENRW 209 >MT0319 [C] COG0778 Nitroreductase MtC Mycobacterium tuberculosis CDC1551 Length = 223 Score = 248 bits (634), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 130/201 (64%), Positives = 159/201 (79%), Gaps = 1/201 (0%) Query: 16 ALYRVIRERRDMRHFLPDPV-DPALLERLLQAAHDGPSVGFMQPWRFIRIASRALRQQIH 74 A+YRVI ERRDMR F+P V +L RLL AAH PSVG MQPWRFIRI L+++IH Sbjct: 9 AVYRVIAERRDMRRFVPGGVVSEDVLARLLHAAHAAPSVGLMQPWRFIRITDETLKRRIH 68 Query: 75 ALVEQERIATAQALGQREDEFMRLKVEGILECGELLVAALMDRREAYVFGRRTLPQMDLA 134 ALV+ ER+ TA+ALG RE+EF+ LKVEGIL+C ELLV AL DRR +Y+FGRRTLPQMDLA Sbjct: 69 ALVDDERLLTAEALGAREEEFLALKVEGILDCAELLVVALCDRRGSYIFGRRTLPQMDLA 128 Query: 135 SLACALQNMWLAARAEGLGMGWVSLFEPQALSHLLHLPSGAEPVAVLCLGHVPAFYDKPM 194 S++CA+QN+WLAAR+EGLGMGWVSLF+PQ L+ LL +P+ AEPVA+LCLG VP F D+P Sbjct: 129 SVSCAIQNLWLAARSEGLGMGWVSLFDPQRLAALLAMPADAEPVAILCLGPVPEFPDRPA 188 Query: 195 LEQQQWAQRQRLQDLVFQDRW 215 LE WA + L + V ++RW Sbjct: 189 LELDGWAYARPLAEFVSENRW 209 >SMc00166 [C] COG0778 Nitroreductase Sme Sinorhizobium meliloti Length = 227 Score = 167 bits (423), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 91/214 (42%), Positives = 127/214 (59%), Gaps = 3/214 (1%) Query: 5 ACHRYPQEQIEALYRVIRERRDMR-HFLPDPVDPALLERLLQAAHDGPSVGFMQPWRFIR 63 A + ++ A+YR I RRD+R FLP+P+ L+ RLL AAH PSVGFMQPW F+ Sbjct: 11 AAGAFSSDERAAVYRAIETRRDVRDEFLPEPLSEELIARLLGAAHQAPSVGFMQPWNFVL 70 Query: 64 IASRALRQQIHALVEQERIATAQAL-GQREDEFMRLKVEGILECG-ELLVAALMDRREAY 121 + R+++ ++ A+ G+R+ ++ LK+EGI + + V R A Sbjct: 71 VRQDETREKVWQAFQRANDEAAEMFSGERQAKYRSLKLEGIRKAPLSICVTCDRTRGGAV 130 Query: 122 VFGRRTLPQMDLASLACALQNMWLAARAEGLGMGWVSLFEPQALSHLLHLPSGAEPVAVL 181 V GR PQMDL S CA+QN+WLAARAEG+G+GWVS+F + +L +P E VA L Sbjct: 131 VLGRTHNPQMDLYSTVCAVQNLWLAARAEGVGVGWVSIFHESEIKAILGIPDHVEIVAWL 190 Query: 182 CLGHVPAFYDKPMLEQQQWAQRQRLQDLVFQDRW 215 CLG V Y +P L + W QR L+DLVF++ W Sbjct: 191 CLGFVDRLYQEPELAAKGWRQRLPLEDLVFEEGW 224