BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2662.p (363 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAB1511 [C] COG1454 Alcohol dehydrogenase, class IV Pab Pyrococc... 130 4e-30 TVN0396 [C] COG1454 Alcohol dehydrogenase, class IV Tvo Thermopl... 129 1e-29 AGl1250 [C] COG1454 Alcohol dehydrogenase, class IV Atu Agrobact... 129 1e-29 AF0024 [C] COG1454 Alcohol dehydrogenase, class IV Afu Archaeogl... 129 1e-29 SA0143_2 [C] COG1454 Alcohol dehydrogenase, class IV Sau Staphyl... 129 2e-29 >PAB1511 [C] COG1454 Alcohol dehydrogenase, class IV Pab Pyrococcus abyssi Length = 375 Score = 130 bits (328), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 105/334 (31%), Positives = 159/334 (47%), Gaps = 16/334 (4%) Query: 2 WHFHNPVTLHAGAGSLAQLPRLLA-GRRALLLTFPDAAALGLEDSVAELLGAQLAGIDNT 60 F T+ G GSL + R+++ G R L+ + LG VA+ + + G+ T Sbjct: 1 MRFFLKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYV-EEAGGVYET 59 Query: 61 V---QPNPDVAWLADMYQRLWQQHADVPCLIALGGGSVIDCAKAM--LAPTPSGRFDEVL 115 + P P V + ++ ++ + + IALGGGSVID AKA+ P FD V Sbjct: 60 IIGLPPEPSVENVEEVLPKVEEFSPE--TFIALGGGSVIDVAKAVKVFYDAPGLDFDSVA 117 Query: 116 AHLREESAFQPEPTRLKSLIAIPTTAGTGSEVTPWATLWDASGGRKYSLHRPWTWAQAAI 175 R + A QP P LIAIP+T+G GSEV+ + + G KY++ P AI Sbjct: 118 IFSRFKKA-QPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAI 174 Query: 176 IDARLMLSLPAATTLASGLDALSHALEAIWNVHRNPVSSTLAVAAARTILVTLPALMRQP 235 +D RL +P SGLD L HA+EA + P S LAV AAR I L + Sbjct: 175 LDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG- 233 Query: 236 RDLALREQMALAALQAGLAFSNTKTALAHSLSYDITLRQGVAHGIACSFSLPHILRLALG 295 D RE++ AA AG+AF N + L H++S+ HG+ + LP+++ + Sbjct: 234 -DAKAREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIG--PHGLVNAIFLPYVMEFNME 290 Query: 296 RDAQVDTLLLQIFDAYSAEQAIARLTGFLEALGV 329 R + + + SA++ + ++ E LGV Sbjct: 291 RAREKYDAMARELGLSSADELLEKVKELNEKLGV 324 >TVN0396 [C] COG1454 Alcohol dehydrogenase, class IV Tvo Thermoplasma volcanium Length = 373 Score = 129 bits (324), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 100/357 (28%), Positives = 166/357 (46%), Gaps = 28/357 (7%) Query: 1 MWHFHNPVTLHAGAGSLAQLPRLLAGRRALLLTFPDAAALGLEDSVAELLGAQLAGIDNT 60 MW+F P + G SL+ L + + L++T + + V L + + + Sbjct: 1 MWYFRTPRIIF-GEDSLSFLDDH-SSSKILVVTDNFLSNTPILKEVLSHLSGKDIKVFSE 58 Query: 61 VQPNPDVAWLADMYQRLWQQHADVPCLIALGGGSVIDCAKAMLAPTPSGRFDEVLAHLRE 120 + P P ++ + + R D +I LGGGS +D AK + A L + + Sbjct: 59 ISPEPRLSEI--LKGRELASRLDPDTIIGLGGGSSMDAAKILFA----------LHEMPD 106 Query: 121 ESAFQPEPT------RLKSLIAIPTTAGTGSEVTPWATLWDASGGRKYSLHRPWTWAQAA 174 S F P + LI IPTT+GTGSE + A D + RK + P A A Sbjct: 107 LSPFDISPINSIKLHQKSELIEIPTTSGTGSECSWAAVFSDDTEERKNEIASPEIMADYA 166 Query: 175 IIDARLMLSLPAATTLASGLDALSHALEAIWNVHRNPVSSTLAVAAARTILVTLPALMRQ 234 I+D +L+L LP T+ + DA++HA+EA + RNP S +A A IL LP ++ Sbjct: 167 ILDPKLVLDLPKGITVNTATDAITHAIEAYTSQWRNPYSDAMAEKALDLILNNLPTVLVN 226 Query: 235 PRDLALREQMALAALQAGLAFSNTKTALAHSLSYDITLRQGVAHGIACSFSLPHILRL-- 292 P+D+ R + + A AG++FSN++ LAH+L + + HG + LPH+ Sbjct: 227 PKDINARNSVHIGASMAGISFSNSQIGLAHALGHALGAIFKTPHGESVGLYLPHVTEFNY 286 Query: 293 --ALGRDAQVDTLLLQIFDAYSAEQAIARLTGFLEALGVSTDPACYGLG-GRWEAAL 346 A R ++++ D Y ++ + +L A+G T+ + G+ ++E L Sbjct: 287 EAAKDRYDKINSFFP---DEYRTDKVWETIRSYLNAIGQVTNVSEAGIERSKYEKEL 340 >AGl1250 [C] COG1454 Alcohol dehydrogenase, class IV Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 392 Score = 129 bits (323), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 108/295 (36%), Positives = 149/295 (50%), Gaps = 17/295 (5%) Query: 7 PVTLHAGAGSLAQLP---RLLAGRRALLLTFPDAAALGLEDSVAELL---GAQLAGIDNT 60 P + G GS +LP + L R LL+ P LG D++ + L G + A + Sbjct: 8 PREMLIGGGSRFRLPAVVKSLGVHRPLLVCDPVLQKLGHADALLQALAEEGIEAAVFSDV 67 Query: 61 VQPNPDVAWLADMYQRLWQQHADVPCLIALGGGSVIDCAKAMLAPTPSGRFDEVLAHLR- 119 V+ D++ +A + +R+ D L+ALGGGS +D AKA SG L R Sbjct: 68 VEDPTDIS-IATLVKRISDGGHD--SLVALGGGSAMDTAKAGAIVATSGEDLRALKVPRI 124 Query: 120 EESAFQPEPTRLKSLIAIPTTAGTGSEVTPWATLWDASGGRKYSLHRPWTWAQAAIIDAR 179 + A P +IAIPTTAGTGSEVT A + D + K + AAIID Sbjct: 125 VDFAVMP-------VIAIPTTAGTGSEVTRAAVVTDTAASEKMLILGTAALPVAAIIDYE 177 Query: 180 LMLSLPAATTLASGLDALSHALEAIWNVHRNPVSSTLAVAAARTILVTLPALMRQPRDLA 239 L L+ P T+ +G+DAL+HALEA+ N + N S LA++A + I L + P D Sbjct: 178 LTLTCPYRVTVDTGIDALTHALEALVNRNGNAHSEALALSALKLIGANLEKVADNPDDRD 237 Query: 240 LREQMALAALQAGLAFSNTKTALAHSLSYDITLRQGVAHGIACSFSLPHILRLAL 294 RE M L A AGLA SNT TAL H LS + V HG++ + LP + + +L Sbjct: 238 AREAMMLGATHAGLAVSNTSTALIHGLSRPVGAFFHVPHGMSNAMVLPLVTQFSL 292 >AF0024 [C] COG1454 Alcohol dehydrogenase, class IV Afu Archaeoglobus fulgidus Length = 416 Score = 129 bits (323), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 99/312 (31%), Positives = 157/312 (50%), Gaps = 28/312 (8%) Query: 4 FHNPVTLHAGAGSLAQLPRLLAGRRALLLTFPDAAALGLEDSVAELL---GAQLAGIDNT 60 F+ L AG++ Q R RRA ++T AL L +V ++L G ++ D Sbjct: 39 FYGLAGLQMFAGAMVQFGR----RRAFIVTDKGVRALAL--TVQKMLKGVGIEVEIFDEA 92 Query: 61 VQPNPDVAWLADMYQRLWQQHADVPCLIALGGGSVIDCAKA--MLAPTPSGRFDEVLAHL 118 +P ++ + +++ D+ ++A+GGGSV+D AKA +L P V Sbjct: 93 -KPEVPLSSIKPAAEKMEALQPDL--IVAVGGGSVMDMAKASWILYEAPQTDLTNV---- 145 Query: 119 REESAFQPEPTRLKSLIA-IPTTAGTGSEVTPWATLWD--ASGGRKYSLHRPWTWAQAAI 175 A QP R K+ +A IPTTAGTGSE T A + D RK + P + A+ Sbjct: 146 ---DAMQPIGLRRKAFLACIPTTAGTGSEATNVAVITDDTVEPHRKEGVVHPELYPDFAV 202 Query: 176 IDARLMLSLPAATTLASGLDALSHALEAIWNVHRNPVSSTLAVAAARTILVTLPALMRQP 235 +D R +L +P TL +GLDALSHA++ N +S LAV A + + LP ++ P Sbjct: 203 LDPRFVLKMPPNLTLGTGLDALSHAVDCYLCRSSNEISDALAVRATKLVFKYLPLALKYP 262 Query: 236 RDLALREQMALAALQAGLAFSNTKTALAHSLSYDITLRQGVAHGIACSFSLPHILRLALG 295 + +R + +AA+ AG AFSN H+++++I V HG+AC ++P++++ Sbjct: 263 DNPQIRLKTQIAAMIAGSAFSNGGIGTTHAVAHNIGALFEVHHGMACGVTIPYVIKFY-- 320 Query: 296 RDAQVDTLLLQI 307 A+VD L Sbjct: 321 --AEVDDKYLSF 330 >SA0143_2 [C] COG1454 Alcohol dehydrogenase, class IV Sau Staphylococcus aureus N315 Length = 411 Score = 129 bits (323), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 105/367 (28%), Positives = 167/367 (45%), Gaps = 26/367 (7%) Query: 4 FHNPVTLHAGAGSLAQLPRLLAGRRALLLTFPDAAALGLEDSVAELLGAQLA----GIDN 59 F P ++ ++ L + + +++ P G +V +L + I + Sbjct: 6 FKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQRTEQPQIKIFS 65 Query: 60 TVQPNPDVAWLADMYQRLWQQHADVPCLIALGGGSVIDCAKAM--LAPTPSGRFDEVLAH 117 V+PNP + + + D +IALGGGS +D AKAM P F Sbjct: 66 EVEPNPSTNTVYKGLEMMVDFQPD--TIIALGGGSAMDAAKAMWMFFEHPETSFFGAKQK 123 Query: 118 L-----REESAFQPEPTRLKSLIAIPTTAGTGSEVTPWATLWDASGGRKYSLHRPWTWAQ 172 R PE + I IPTT+GTGSEVTP+A + D+ KY L Sbjct: 124 FLDIGKRTYKIGMPENA---TFICIPTTSGTGSEVTPFAVITDSETNVKYPLADFALTPD 180 Query: 173 AAIIDARLMLSLPAATTLASGLDALSHALEAIWNVHRNPVSSTLAVAAARTILVTLPALM 232 AIID + ++S+P + T +G+D L+HA+E+ +V + + L++ A + L + + Sbjct: 181 VAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYLKSSV 240 Query: 233 RQPRDLALREQMALAALQAGLAFSNTKTALAHSLSYDITLRQGVAHGIACSFSLPHILRL 292 + D RE+M A+ AG+AF+N +AHS+++ I G+ HG A + LPHI+R Sbjct: 241 EK-GDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYGIPHGRANAILLPHIIRY 299 Query: 293 ALGRDAQVDTLLLQIFDAYSAEQAIARLTGFL-------EALGVSTDPACYGLGGRWEAA 345 +D Q L + ++ + A+ A + FL EAL S A Y LG Sbjct: 300 N-AKDPQKHALFPK-YEFFRADTDYADIAKFLGLKGNTTEALVESLAKAVYELGQSVGIE 357 Query: 346 LQAALQG 352 + QG Sbjct: 358 MNLKSQG 364