BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2316.p (405 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO3224 [R] COG1073 Hydrolases of the alpha/beta superfamily Ype... 67 8e-11 MA2002 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 67 1e-10 STM0318 [R] COG1073 Hydrolases of the alpha/beta superfamily Sty... 65 4e-10 mlr6657 [R] COG1073 Hydrolases of the alpha/beta superfamily Mlo... 61 4e-09 ZyafA [R] COG1073 Hydrolases of the alpha/beta superfamily EcZ E... 61 5e-09 >YPO3224 [R] COG1073 Hydrolases of the alpha/beta superfamily Ype Yersinia pestis Length = 415 Score = 67.0 bits (162), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 85/344 (24%), Positives = 139/344 (40%), Gaps = 22/344 (6%) Query: 65 LIESLQGLDGASWAAVWGKAGDETRAQADLASERGQDKEAAALYMKASGFYFMGRFPCPN 124 L++++ G +W W G + +A ++ G A ++ A+ Y + +P + Sbjct: 85 LLDTVVGYRNGNWIYEWANQGMMWQQKAMEETDPGS---AGQFWLNAANLYSIASYP--H 139 Query: 125 HPDKERCAQAE---RETYVLAASYWDNPVRRVTIAF-DGRSGEGKEVVFLIRRPHGVERP 180 E QAE Y AA Y ++ +T DG S G FL G Sbjct: 140 LKGDELSEQAEVLSNRAYEEAAKYLPYTLKELTFPISDGGSLSG----FLHMPTVGSAPF 195 Query: 181 PVVVMWGGVDAWKEQMTAACNAFLA-AGVATIAMDNAGTGESPV-KGVQDAERQFLPVFE 238 P V+M GG+D + +L G+A + +D G S K QD V + Sbjct: 196 PTVLMCGGLDTLQSDYHRLFRDYLEPKGIAMLTIDLPSVGASSRWKLTQDTSYLHQQVLQ 255 Query: 239 WAAAQEDLDAAKVGILGRSFGGYWATKLAHLHPERISAAVNWGGGAHYMF-QADWIESSR 297 A +D +V + G FG A +L +L P+R+ A G H++ +D + + Sbjct: 256 ALADVPWVDHQRVSVFGFRFGANVAVRLGYLEPQRVRAVACLGPIVHHLLCNSDSLR--K 313 Query: 298 YPDSYLMELVETRQRMLGATTDQQYIDGFRRLSLLDQGLL-DRPSAPMLLVNGKEDKQCP 356 PD Y M+++ +R M +TD+ R SL QGLL R PML + D P Sbjct: 314 VPDMY-MDVMASRLGM-ADSTDETLNTEMNRYSLKTQGLLGRRCQTPMLAGFWENDPFSP 371 Query: 357 PADLHLLTEHGSPKAVRLFPGGHM-GNTPQTLPTIVQWLSTHVK 399 + L+ + + P + N + L +WL ++ Sbjct: 372 KEEAKLICSSSADGKLLAIPSKPLYENFHRALLQTSEWLEDKMR 415 >MA2002 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Mac Methanosarcina acetivorans str.C2A Length = 417 Score = 66.6 bits (161), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 57/220 (25%), Positives = 92/220 (41%), Gaps = 17/220 (7%) Query: 73 DGASWAAVWGKAGDETRAQADLASERGQDKEAAALYMKASGFYFMGRFPCPNHPDKERCA 132 D SW W + D R + A Y++AS +Y F +PD R Sbjct: 59 DIESWYLEWNRTAGRLETTGDEFLARSNNVSARETYLRASNYYRTAGFYLTKNPDDPRIV 118 Query: 133 ---QAERETYVLAASYWDNPVRRVTIAFDGRSGEGKEVVFLIRRPHGVERPPVVVMWGGV 189 + R+ ++ AA +N +R V I F+ + G +L E+ P++++ G Sbjct: 119 PTWERSRDCFLKAADLSNNLIRPVRIPFENTTLPG----YLCLVDTDGEKRPLLIVQTGY 174 Query: 190 DAWKEQMTAACNAF-LAAGVATIAMDNAGTGESPVKGVQDA------ERQFLPVFEWAAA 242 D E++ F + G + + G GE V VQ E PV +A + Sbjct: 175 DGTGEELYFQVAKFAVDRGYNVLIFEGPGQGE--VIRVQKIPFRYNWESVVTPVTNYALS 232 Query: 243 QEDLDAAKVGILGRSFGGYWATKLAHLHPERISAAVNWGG 282 Q +D ++ ++G SFGGY A + A + RI A + GG Sbjct: 233 QSIVDPERIVLMGISFGGYLAPR-AVVFEHRIKACIANGG 271 >STM0318 [R] COG1073 Hydrolases of the alpha/beta superfamily Sty Salmonella typhimurium LT2 Length = 414 Score = 64.7 bits (156), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 84/336 (25%), Positives = 133/336 (39%), Gaps = 15/336 (4%) Query: 65 LIESLQGLDGASWAAVWGKAGDETRAQADLASERGQDKEAAALYMKASGFYFMGRFPCPN 124 L +++ G G +W W K + + +A + + ++ AS Y + +P Sbjct: 85 LYDTVIGYRGGNWIYEWAKQAMDWQQKA---CQEQDAMRSGRYWLHASTLYNIAAYPHLK 141 Query: 125 HPDKERCAQA-ERETYVLAASYWDNPVRRVTIAFDGRSGEGKEVVFLIRRPHGVERPPVV 183 + AQA Y AA +R + A G G V + P G P V Sbjct: 142 GDELAEQAQALANRAYEEAAQRLPGSLREMEFAVPG----GSPVTAFLHMPKGDGPFPTV 197 Query: 184 VMWGGVDAWKEQMTAACNAFLAA-GVATIAMDNAGTG-ESPVKGVQDAERQFLPVFEWAA 241 +M GG+DA + + A G+A + +D G S K QD+ V + Sbjct: 198 LMCGGLDAMQTDYYTLYERYFAPRGIAMLTLDMPSVGFSSKWKLTQDSSLLHQHVLKALP 257 Query: 242 AQEDLDAAKVGILGRSFGGYWATKLAHLHPERISAAVNWGGGAHYMFQADWIESSRYPDS 301 +D +V G FG A +LA+L R+ A G H + +D S P+ Sbjct: 258 NVPWVDHTRVAAFGFRFGANVAVRLAYLEAPRLKAVACLGPVVHALL-SDPQRQSTVPEM 316 Query: 302 YLMELVETRQRMLGATTDQQYIDGFRRLSLLDQGLLDRPS-APMLLVNGKEDKQCPPADL 360 YL +++ +R M A+ + ++ R SL QGLL R PML K D P + Sbjct: 317 YL-DVLASRLGMHDASDEALRVE-LNRYSLKVQGLLGRRCPTPMLSGFWKNDPFSPEEES 374 Query: 361 HLLTEHGSPKAVRLFPGGHM-GNTPQTLPTIVQWLS 395 L+T S + P + N + L I W++ Sbjct: 375 RLITTSSSDGKLIEIPFNPVYRNFDRALQEITDWIN 410 >mlr6657 [R] COG1073 Hydrolases of the alpha/beta superfamily Mlo Mesorhizobium loti Length = 295 Score = 61.2 bits (147), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 80/297 (26%), Positives = 125/297 (42%), Gaps = 39/297 (13%) Query: 128 KERCAQAERETYVLAASYWDNPVRRVTIAFDGRSGEGKEVVFLIRRPHGVERPPVVVMWG 187 K R + ER T V A++Y D + V I DGR G ++ RP G+ P VV+M Sbjct: 2 KSRFIKDER-TVVHASNYGDAGEKVVRIDSDGRGLAG-----ILNRPCGIPAPAVVLMLH 55 Query: 188 GVDAWKEQMTAACN-----AFLAA-----GVATIAMDNAGTGESPVKGVQDA----ERQF 233 G K + A A+ AA G+AT+ +D G+G+S + Sbjct: 56 GFTGQKNEFQLAKTGIGLFAYAAAKLAEQGIATLRIDFNGSGDSAGNWIDTTFSGQINDA 115 Query: 234 LPVFEWAAAQEDLDAAKVGILGRSFGGYWATKLAHLHPERISAAVNWGGGAHYMFQADWI 293 + +++ D+D ++VGILG S GG A+ +A L P+ SA V W + M + Sbjct: 116 MLAYDYLQTLRDVDGSRVGILGYSQGGLVASHVAALRPQA-SALVLWAPVTNPMSTFSSL 174 Query: 294 ----ESSRYPDSYLMELVETRQRMLGATTDQQYIDGFRRLSLLDQGL-LDRPSAPMLLVN 348 +R E + T+ G TT + ++ L L + + R P+ +V Sbjct: 175 VGAETVARAIAGEASEEIRTKLSWGGETTLKAAF--YKELPLFAPAVAISRYHGPLRVVV 232 Query: 349 GKEDKQCPPADLH---LLTEHGSPKAVRLFPGGHMGN---TPQT-----LPTIVQWL 394 G+ + P LLT HG + GH N +P T +P+ V+W Sbjct: 233 GRREMIVAPQPAEGRSLLTHHGGVNDLVEVDSGHDWNAETSPDTVENVLIPSTVEWF 289 >ZyafA [R] COG1073 Hydrolases of the alpha/beta superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 414 Score = 60.8 bits (146), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 78/308 (25%), Positives = 125/308 (40%), Gaps = 22/308 (7%) Query: 65 LIESLQGLDGASWAAVWGKAGDETRAQADLASERGQDKEAAAL----YMKASGFYFMGRF 120 L +++ G G +W W QA + ++ +E L ++ A+ Y + + Sbjct: 85 LYDTVIGYRGGNWIYEWA-------TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAY 137 Query: 121 PCPNHPDKERCAQA-ERETYVLAASYWDNPVRRVTIAFDGRSGEGKEVVFLIRRPHGVER 179 P D AQA Y AA +R++ G G + + P G Sbjct: 138 PHLKGDDLAEQAQALSNRAYEEAAQRLPGTMRQMEFTVPG----GAPITGFLHMPKGDGP 193 Query: 180 PPVVVMWGGVDAWKEQMTAACNAFLAA-GVATIAMDNAGTG-ESPVKGVQDAERQFLPVF 237 P V+M GG+DA + + + A G+A + +D G S K QD+ V Sbjct: 194 FPTVLMCGGLDAMQTDYYSLYERYFAPRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVL 253 Query: 238 EWAAAQEDLDAAKVGILGRSFGGYWATKLAHLHPERISAAVNWGGGAHYMFQADWIESSR 297 + +D +V G FG A +LA+L R+ A G H + +D+ + Sbjct: 254 KALPNVPWVDHTRVAAFGFRFGANVAVRLAYLESPRLKAVACLGPVVHTLL-SDFKCQQQ 312 Query: 298 YPDSYLMELVETRQRMLGATTDQQYIDGFRRLSLLDQGLLDRPS-APMLLVNGKEDKQCP 356 P+ YL +++ +R M A+ + ++ R SL QGLL R PML K D P Sbjct: 313 VPEMYL-DVLASRLGMHDASDEALRVE-LNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSP 370 Query: 357 PADLHLLT 364 D L+T Sbjct: 371 EEDSRLIT 378