BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2012.p (296 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1388 [J] COG2890 Methylase of polypeptide chain release facto... 373 e-103 NMA1912 [J] COG2890 Methylase of polypeptide chain release facto... 351 9e-97 NMB1655 [J] COG2890 Methylase of polypeptide chain release facto... 351 1e-96 PA1678 [J] COG2890 Methylase of polypeptide chain release factor... 281 9e-76 STM2385 [J] COG2890 Methylase of polypeptide chain release facto... 275 7e-74 >RSc1388 [J] COG2890 Methylase of polypeptide chain release factors Rso Ralstonia solanacearum Length = 304 Score = 373 bits (958), Expect = e-103, Method: Compositional matrix adjust. Identities = 185/289 (64%), Positives = 223/289 (77%), Gaps = 1/289 (0%) Query: 6 FSTVRDLLRYAVTRFNTEQLFFGHGSSNALDEAAYLILHTLKLPLDQLDPFLDARLLPEE 65 F+T+RDLLRYAV+RF L FGHGS NA DEAAYLILHTL LPLD L+PFLDARLLPEE Sbjct: 16 FTTMRDLLRYAVSRFTAAGLVFGHGSENAYDEAAYLILHTLHLPLDTLEPFLDARLLPEE 75 Query: 66 IASIMRVIERRSKDRVPAAYITNEAWLGAYRFYVDERVIVPRSFIAELIPQHFSPWIQDP 125 +A++++VIERR+ DRVPAAY+T+EA++ RFYVDERVIVPRSFI EL+ + +PW+ Sbjct: 76 VAAVLQVIERRTVDRVPAAYLTHEAYMHGMRFYVDERVIVPRSFIGELLEEGLAPWLPHE 135 Query: 126 EAIENALDLCTGSGCLPILLADAFPNAQIDAVDISADALAVARKNVDEYQLQDRINLVQS 185 + + L+LCTGSGCL IL A +PNA +DAVD+S DAL VA++NVD+Y L I L + Sbjct: 136 DGPTDVLELCTGSGCLAILAALQWPNATLDAVDLSPDALVVAQRNVDDYGLNGCIRLHEG 195 Query: 186 DLYTQLPLR-KYDLIISNPPYVNSGSMAKLPQEYLREPQLALAGGEDGMDLVRKIVAGAA 244 DLY LP YD+I++NPPYVN SM LP EY EP++ALAGG DGMD+VR+I+A A Sbjct: 196 DLYAPLPPGVHYDVILTNPPYVNETSMQALPPEYRAEPRMALAGGTDGMDIVRRILADAP 255 Query: 245 ERLTPEGVLVVEIGNERAFAEAAFPELELTWLTTSAGDDMVFLVTAEQL 293 L P GVLVVEIGNER EAAFP+L+L WL TSAGDD VFLVT E L Sbjct: 256 RHLKPHGVLVVEIGNERENVEAAFPDLDLVWLPTSAGDDQVFLVTREAL 304 >NMA1912 [J] COG2890 Methylase of polypeptide chain release factors NmA Neisseria meningitidis Z2491 Length = 303 Score = 351 bits (901), Expect = 9e-97, Method: Compositional matrix adjust. Identities = 170/288 (59%), Positives = 217/288 (75%), Gaps = 1/288 (0%) Query: 6 FSTVRDLLRYAVTRFNTEQLFFGHGSSNALDEAAYLILHTLKLPLDQLDPFLDARLLPEE 65 +T+RD+LR+AV+RFN LFFGHG+ NA DEAAYLILHTL LPLD L P+LDA+LL E Sbjct: 13 LTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYLILHTLNLPLDMLAPYLDAKLLEAE 72 Query: 66 IASIMRVIERRSKDRVPAAYITNEAWLGAYRFYVDERVIVPRSFIAELIPQHFSPWIQDP 125 ++ VIERR+ + +PAAY+T++AW G + FYVDERVI+PRSFI EL+ PWI+ Sbjct: 73 KEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYVDERVIIPRSFIYELLGDGLRPWIEYD 132 Query: 126 EAIENALDLCTGSGCLPILLADAFPNAQIDAVDISADALAVARKNVDEYQLQDRINLVQS 185 E + NALDLCTGSGCL I +A +P+AQIDAVD+S DAL VA NV++Y L++RI L+ + Sbjct: 133 ELVHNALDLCTGSGCLAIQMAHHYPDAQIDAVDVSLDALEVAGINVEDYGLEERIRLIHT 192 Query: 186 DLYTQLPLRKYDLIISNPPYVNSGSMAKLPQEYLREPQLALAGGEDGMDLVRKIVAGAAE 245 DL+ L YDLI+SNPPYV++ S+ LP+EYL EP+LAL G DG+D R+I+ AA+ Sbjct: 193 DLFEGLE-GTYDLIVSNPPYVDAESVELLPEEYLHEPELALGSGADGLDATRQILLNAAK 251 Query: 246 RLTPEGVLVVEIGNERAFAEAAFPELELTWLTTSAGDDMVFLVTAEQL 293 L P+GVL+VEIG+ R EAA+PEL TWL TS GD VFL+T EQL Sbjct: 252 FLNPKGVLLVEIGHNRDVLEAAYPELPFTWLETSGGDGFVFLLTREQL 299 >NMB1655 [J] COG2890 Methylase of polypeptide chain release factors Nme Neisseria meningitidis MC58 Length = 303 Score = 351 bits (901), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 170/288 (59%), Positives = 217/288 (75%), Gaps = 1/288 (0%) Query: 6 FSTVRDLLRYAVTRFNTEQLFFGHGSSNALDEAAYLILHTLKLPLDQLDPFLDARLLPEE 65 +T+RD+LR+AV+RFN LFFGHG+ NA DEAAYLILHTL LPLD L P+LDA+LL E Sbjct: 13 LTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYLILHTLNLPLDMLAPYLDAKLLEAE 72 Query: 66 IASIMRVIERRSKDRVPAAYITNEAWLGAYRFYVDERVIVPRSFIAELIPQHFSPWIQDP 125 ++ VIERR+ + +PAAY+T++AW G + FYVDERVI+PRSFI EL+ PWI+ Sbjct: 73 KEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYVDERVIIPRSFIYELLGDGLRPWIEYD 132 Query: 126 EAIENALDLCTGSGCLPILLADAFPNAQIDAVDISADALAVARKNVDEYQLQDRINLVQS 185 E + NALDLCTGSGCL I +A +P+AQIDAVD+S DAL VA NV++Y L++RI L+ + Sbjct: 133 ELVHNALDLCTGSGCLAIQMAHHYPDAQIDAVDVSLDALEVAGINVEDYGLEERIRLIHT 192 Query: 186 DLYTQLPLRKYDLIISNPPYVNSGSMAKLPQEYLREPQLALAGGEDGMDLVRKIVAGAAE 245 DL+ L YDLI+SNPPYV++ S+ LP+EYL EP+LAL G DG+D R+I+ AA+ Sbjct: 193 DLFEGLE-GTYDLIVSNPPYVDAESVELLPEEYLHEPELALGSGADGLDATRQILLNAAK 251 Query: 246 RLTPEGVLVVEIGNERAFAEAAFPELELTWLTTSAGDDMVFLVTAEQL 293 L P+GVL+VEIG+ R EAA+PEL TWL TS GD VFL+T EQL Sbjct: 252 FLNPKGVLLVEIGHNRDVLEAAYPELPFTWLETSGGDGFVFLLTREQL 299 >PA1678 [J] COG2890 Methylase of polypeptide chain release factors Pae Pseudomonas aeruginosa Length = 304 Score = 281 bits (720), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 136/294 (46%), Positives = 198/294 (67%), Gaps = 2/294 (0%) Query: 1 MTPNNFSTVRDLLRYAVTRFNTEQLFFGHGSSNALDEAAYLILHTLKLPLDQLDPFLDAR 60 M+ + T+RD +R+AV+RF+ LFFGHG+ NA DEA +L+L +L LP + D +LD R Sbjct: 1 MSESRLLTLRDYIRWAVSRFHAAGLFFGHGTDNAWDEARHLVLGSLHLPWEISDSYLDCR 60 Query: 61 LLPEEIASIMRVIERRSKDRVPAAYITNEAWLGAYRFYVDERVIVPRSFIAELIPQHFSP 120 L +E A + ++ RR ++R+PAAY+ EAW F VDERV+VPRS IAELI Q F+P Sbjct: 61 LEDDERAELAEILRRRIEERIPAAYLLGEAWFCGIPFSVDERVLVPRSPIAELIEQRFAP 120 Query: 121 WIQDPEAIENALDLCTGSGCLPILLADAFPNAQIDAVDISADALAVARKNVDEYQLQDRI 180 W+ P LDLCTGSGC+ I A AF A++ D+S DAL VA N++ + L +R+ Sbjct: 121 WL--PAEPARILDLCTGSGCIGIACAYAFEQAEVVLADLSFDALEVANVNIERHDLGERV 178 Query: 181 NLVQSDLYTQLPLRKYDLIISNPPYVNSGSMAKLPQEYLREPQLALAGGEDGMDLVRKIV 240 VQ D + LP +++DLI+SNPPYV++ A +P E+ EP+L LA G+DG+DLVR+++ Sbjct: 179 YTVQGDGFAGLPGQRFDLIVSNPPYVDAEDFADMPAEFHHEPELGLACGDDGLDLVRRML 238 Query: 241 AGAAERLTPEGVLVVEIGNERAFAEAAFPELELTWLTTSAGDDMVFLVTAEQLQ 294 A AA+ L+ +G+L+VE+GN +A +PE++ TWL G VF+++A Q + Sbjct: 239 AEAADHLSEKGLLIVEVGNSEVHVQALYPEVDFTWLEFEHGGHGVFMLSAAQCR 292 >STM2385 [J] COG2890 Methylase of polypeptide chain release factors Sty Salmonella typhimurium LT2 Length = 310 Score = 275 bits (704), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 136/291 (46%), Positives = 192/291 (65%), Gaps = 4/291 (1%) Query: 4 NNFSTVRDLLRYAVTRFNTEQLFFGHGSSNALDEAAYLILHTLKLPLDQLDPFLDARLLP 63 + T++D+LR+AV+RF+ +++GHG+ N DEA L+L +L LPLD + ARL Sbjct: 11 SELHTIQDMLRWAVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIPEDMRTARLTS 70 Query: 64 EEIASIMRVIERRSKDRVPAAYITNEAWLGAYRFYVDERVIVPRSFIAELIPQHFSPWI- 122 E I+ + RR +R+P AY+TN+AW + FYVDERV+VPRS I ELI HF+ I Sbjct: 71 SEKHRIVERVIRRINERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGELINNHFAGLIS 130 Query: 123 QDPEAIENALDLCTGSGCLPILLADAFPNAQIDAVDISADALAVARKNVDEYQLQDRINL 182 Q P+ I LD+CTGSGC+ I A AFP+A++DAVDIS DALAVA N++E+ L + Sbjct: 131 QQPKYI---LDMCTGSGCIAIACAYAFPDAEVDAVDISPDALAVAEHNIEEHGLIHHVTP 187 Query: 183 VQSDLYTQLPLRKYDLIISNPPYVNSGSMAKLPQEYLREPQLALAGGEDGMDLVRKIVAG 242 ++SDL+ LP +YDLI++NPPYV++ M+ LP EY EP+L LA G DG+ L R+I+ Sbjct: 188 IRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKLTRRILGN 247 Query: 243 AAERLTPEGVLVVEIGNERAFAEAAFPELELTWLTTSAGDDMVFLVTAEQL 293 A + L+ +GVL+ E+GN +P++ TWL G D VF++T QL Sbjct: 248 APDYLSDDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKAQL 298