BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2000.p (334 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1369 [L] COG1573 Uracil-DNA glycosylase Rso Ralstonia solanac... 225 1e-58 TP0229 Non COG 148 2e-35 AF2277 [L] COG1573 Uracil-DNA glycosylase Afu Archaeoglobus fulg... 145 1e-34 CAC2997 [L] COG1573 Uracil-DNA glycosylase Cac Clostridium aceto... 141 2e-33 BMEI1321 [L] COG1573 Uracil-DNA glycosylase Bme Brucella melitensis 137 4e-32 >RSc1369 [L] COG1573 Uracil-DNA glycosylase Rso Ralstonia solanacearum Length = 292 Score = 225 bits (574), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 110/196 (56%), Positives = 134/196 (68%), Gaps = 6/196 (3%) Query: 134 IDPLIARIKTMDWPQLKQQVADCRRCGLCQGRKNTVFGVGDEKAKWLFIGEGPGRNEDQQ 193 + P RI DW QL+ V+ C C LC+ R TVFGVGD +A W+ IGE PG ED+Q Sbjct: 97 VAPRAQRIAAFDWAQLEAAVSGCTACKLCERRTQTVFGVGDRQADWMLIGEAPGEQEDRQ 156 Query: 194 GEPFVGPAGKLLDNMLLSMGVKRGENAYIANIVKCRPTDDNGRDRPPSPQEVASCLPYLQ 253 GEPFVG AGKLLD+ML ++G+ R +IAN++KCRP +R P P EVA C PYL+ Sbjct: 157 GEPFVGQAGKLLDSMLRAIGLSRETGVFIANVLKCRPPG----NRDPEPDEVAMCDPYLK 212 Query: 254 RQIELIQPTVLVALGKTAAISLLGMDPTTPVSRLRGTVHRYQELPLVVTYHPAYLLRTLN 313 RQI LI+P V+V LG+ AA SLL TPV +LRG VH +P+VVTYHPAYLLRTL Sbjct: 213 RQIALIKPRVIVVLGRFAAQSLL--QTQTPVGKLRGKVHEVDGVPVVVTYHPAYLLRTLT 270 Query: 314 DKSKAWADLCLAMSTY 329 DK++AW DLCLA Y Sbjct: 271 DKARAWEDLCLARKVY 286 >TP0229 Non COG Length = 277 Score = 148 bits (373), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 78/184 (42%), Positives = 108/184 (58%), Gaps = 10/184 (5%) Query: 149 LKQQVADCRRCGLCQGRKNTVFGVGDEKAKWLFIGEGPGRNEDQQGEPFVGPAGKLLDNM 208 L++++ CR C L Q R + V G G A L +GE PG ED+ G PFVG +GKLLD M Sbjct: 92 LREEIFACRACELYQRRTHAVVGEGVADADVLVVGEAPGAEEDRSGRPFVGRSGKLLDAM 151 Query: 209 LLSMGVKRGENAYIANIVKCRPTDDNGRDRPPSPQEVASCLPYLQRQIELIQPTVLVALG 268 L ++G+ R +N YI N+VKCRP R+R P+P E A C +L + L +P ++ LG Sbjct: 152 LAAIGLSRQQNCYITNVVKCRPP----RNRTPTPHETACCARFLHAHLTLHRPCAILVLG 207 Query: 269 KTAAISLLGMDPTTPVSRLRGTVHRYQELPLVVTYHPAYLLRTLNDKSKAWADLCLAMST 328 + AA +L T + +LRG YQ +PL+ TYHP+ LLR K AW DL T Sbjct: 208 RCAAQHML--QTTDGIGKLRGRFFTYQGIPLLATYHPSALLRDEALKRPAWEDL----KT 261 Query: 329 YQAQ 332 ++A+ Sbjct: 262 FRAR 265 >AF2277 [L] COG1573 Uracil-DNA glycosylase Afu Archaeoglobus fulgidus Length = 199 Score = 145 bits (366), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 75/166 (45%), Positives = 114/166 (68%), Gaps = 13/166 (7%) Query: 151 QQVADCRRCGLCQGRKNTVFGVGDEKAKWLFIGEGPGRNEDQQGEPFVGPAGKLLDNMLL 210 +++ CR+C L + + N V GVG+EKA+ +F+GE PGR+ED +GEPFVG AGKLL ML Sbjct: 9 REIMSCRKCDLHKTKTNYVPGVGNEKAEIVFVGEAPGRDEDLKGEPFVGAAGKLLTEMLA 68 Query: 211 SMGVKRGENAYIANIVKCRPTDDNGRDRPPSPQEVASCLPYLQRQIELIQPTVLVALGKT 270 S+G++R E+ YI N++KCRP + +R P+P+EV C YL RQ+E I+P V+V LG+ Sbjct: 69 SIGLRR-EDVYITNVLKCRPPN----NRDPTPEEVEKCGDYLVRQLEAIRPNVIVCLGRF 123 Query: 271 AA---ISLLGMDPTTPVSRLRGTVHRYQ----ELPLVVTYHPAYLL 309 AA +L ++ TT +SR++G V+ + ++ ++ YHPA +L Sbjct: 124 AAQFIFNLFDLEFTT-ISRVKGKVYEVERWGKKVKVIAIYHPAAVL 168 >CAC2997 [L] COG1573 Uracil-DNA glycosylase Cac Clostridium acetobutylicum Length = 206 Score = 141 bits (356), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 73/206 (35%), Positives = 112/206 (54%), Gaps = 24/206 (11%) Query: 144 MDWPQLKQQVADCRRCGLCQGRKNTVFGVGDEKAKWLFIGEGPGRNEDQQGEPFVGPAGK 203 + W +L + +C++C L + R N VFG G+ +AK +F+GE PG +ED+ G PFVG AG+ Sbjct: 2 LQWRELYNECIECQKCRLGENRTNMVFGEGNPEAKLMFVGEAPGADEDRLGRPFVGRAGQ 61 Query: 204 LLDNMLLSMGVKRGENAYIANIVKCRPTDDNGRDRPPSPQEVASCLPYLQRQIELIQPTV 263 LL L ++ + R + YIAN+ KCRP ++R P E +C+PYL+ Q LI+P + Sbjct: 62 LLTKGLTALNLSRDRDYYIANVCKCRPE----KNRTPYEDEAEACMPYLRNQFALIKPKI 117 Query: 264 LVALGKTAAI--------------------SLLGMDPTTPVSRLRGTVHRYQELPLVVTY 303 LV LG TA +L+ + ++R RG + + + ++ T+ Sbjct: 118 LVCLGSTAMKFIMEPALNKILQQNNTIEEENLIWKNGQMRITRDRGKWLKVKGIYMMATF 177 Query: 304 HPAYLLRTLNDKSKAWADLCLAMSTY 329 HPA L R N K+ W DL L + Y Sbjct: 178 HPAALFRDENKKTAFWEDLKLIKAKY 203 >BMEI1321 [L] COG1573 Uracil-DNA glycosylase Bme Brucella melitensis Length = 290 Score = 137 bits (344), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 83/192 (43%), Positives = 113/192 (58%), Gaps = 13/192 (6%) Query: 148 QLKQQVADCRRCGLCQGRKNTVFGVGDEKAKWLFIGEGPGRNEDQQGEPFVGPAGKLLDN 207 +L++++A C L KN F GD + +FIGE PGR+ED +G PFVG +G+LL+ Sbjct: 106 ELREKLAAFDGCNLKFTAKNLCFADGDPSSDIMFIGEAPGRDEDMEGLPFVGKSGQLLNR 165 Query: 208 MLLSMGVKRGENAYIANIVKCRPTDDNGRDRPPSPQEVASCLPYLQRQIELIQPTVLVAL 267 M+ ++G+KR E YIAN + RP +R P+P E C P+++RQIEL P VLVAL Sbjct: 166 MIEAIGLKR-EEVYIANTIPWRPPG----NRAPTPLETELCRPFIERQIELAAPKVLVAL 220 Query: 268 GKTAAISLLGMDPTTPVSRLRGT--VHRY---QELPLVVTYHPAYLLRTLNDKSKAWADL 322 G A +L G + RLRG +HR E+P++ T HPAYLLRT K AW D Sbjct: 221 GGPAGKALTGA--AEGILRLRGNWKIHRTPTGMEIPVMPTLHPAYLLRTPAQKRFAWRDF 278 Query: 323 CLAMSTYQAQAR 334 LA+ A+ R Sbjct: 279 -LAVKLKLAELR 289