BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1958.p (324 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3892 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomo... 365 e-101 PA2430 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomo... 256 4e-68 ydhJ [V] COG1566 Multidrug resistance efflux pump Eco Escherichi... 236 4e-62 Z2659 [V] COG1566 Multidrug resistance efflux pump EcZ Escherich... 235 1e-61 ECs2353 [V] COG1566 Multidrug resistance efflux pump Ecs Escheri... 235 1e-61 >PA3892 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomonas aeruginosa Length = 302 Score = 365 bits (937), Expect = e-101, Method: Compositional matrix adjust. Identities = 187/322 (58%), Positives = 240/322 (74%), Gaps = 28/322 (8%) Query: 1 MPVKSIFRVLITLVIFLVAILLAWSLWQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALP 60 M +KS+ +L TL+I LVA+ + +LW +YM +PWTRDGRVRADV+N+A DVSG VV +P Sbjct: 1 MTLKSVISLLATLLILLVAVFIGRTLWVNYMDTPWTRDGRVRADVINVAADVSGIVVDVP 60 Query: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKADLEVRKAEVEARRADLNMRRAQA 120 VRDNQLVKKGDLLM+IDP Y +A++QAE+ V +RKA LE MR+ A Sbjct: 61 VRDNQLVKKGDLLMQIDPDHYRIAVKQAESLVASRKATLE--------------MRQLNA 106 Query: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQYQAALSQRDTAKLNLER 180 RRRA++D +VVSRE+R+DA + A+ A E A+AQL D A+LNLER Sbjct: 107 RRRAEMDEMVVSRESRDDAHNTAAAAMADYEQAKAQL--------------DAARLNLER 152 Query: 181 TKVFSPVDGYITNLNVRRGDYATAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDHAKIQ 240 T+V + VDGY+TNLNV RGDYA G AK+AVID +S+WVY YFEETKLP + GD +Q Sbjct: 153 TRVVAQVDGYVTNLNVHRGDYARVGEAKMAVIDKNSYWVYGYFEETKLPYIREGDPVDMQ 212 Query: 241 LINGATMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIHLDKVPENVL 300 L++G ++GHV+SIARGIYDRDNP+SRELTADVNPTFNWVRLAQR+PVR+H+D+VP+ VL Sbjct: 213 LMSGEHLKGHVESIARGIYDRDNPESRELTADVNPTFNWVRLAQRVPVRVHIDEVPDGVL 272 Query: 301 LASGLTCTVVLEPGSGKQQAVA 322 L++G+TCTV+++P QA A Sbjct: 273 LSAGITCTVIVKPQGRDDQASA 294 >PA2430 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomonas aeruginosa Length = 334 Score = 256 bits (654), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 135/289 (46%), Positives = 193/289 (66%), Gaps = 2/289 (0%) Query: 27 WQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALPVRDNQLVKKGDLLMEIDPARYTLALQ 86 W+ Y +PWTRD RVRADVV ++ DVSG + LP+ DNQ V+KG+LL+ IDPARY LA+ Sbjct: 28 WERYTRTPWTRDARVRADVVTLSADVSGLITRLPLSDNQPVEKGELLLSIDPARYELAVL 87 Query: 87 QAEAAVTARKADLEVRKAEVEARRADLNMRRAQARRRADVDAL-VVSREAREDAGSQVTA 145 +AE AV +A L +A +EA +A L RR++A+RR+ + ++S E E A + V+ Sbjct: 88 RAERAVNVARAALGESRAAIEASQAQLRQRRSEAQRRSALQKRSMLSVEENEKAQTDVSL 147 Query: 146 SQAQLEAAQAQLQGAQAQYQAALSQRDTAKLNLERTKVFSPVDGYITNLNVRRGDYATAG 205 +QA+L QA L AQA + A + A+L+LERT+V +PV GY+TNL R GDYA AG Sbjct: 148 AQAELLRNQASLGLAQANVELAEAALQQARLDLERTQVRAPVSGYVTNLQTREGDYAHAG 207 Query: 206 AAKIAVIDSHSFWVYAYFEETKLPLLSIGDHAKIQLINGATMEGHVDSIARGIYDRDNPQ 265 +A++D SF+V YFEETKLP + +G A+++L++G +G V SIA I DR+N + Sbjct: 208 VPLLALVDRDSFYVSGYFEETKLPGIHVGSRARVELMSGERFDGRVQSIAHAITDRENAE 267 Query: 266 SRELTADVNPTFNWVRLAQRIPVRIHLDKV-PENVLLASGLTCTVVLEP 313 L A++NP++ WV+LAQRIPVRI +D + L +G+T T+ + P Sbjct: 268 GSRLLANINPSYTWVKLAQRIPVRIAIDPAYRQRNTLRAGVTATIRVLP 316 >ydhJ [V] COG1566 Multidrug resistance efflux pump Eco Escherichia coli K12 Length = 299 Score = 236 bits (603), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 134/312 (42%), Positives = 185/312 (59%), Gaps = 30/312 (9%) Query: 1 MPVKSIFRVLITLVIFLVAILLAWSLWQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALP 60 M +K+I + T+++ +VA+L W LW +YM SPWTRDG++RA+ V+I P VSG++V L Sbjct: 15 MSIKTI-KYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQVSITPQVSGRIVELN 73 Query: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKADLEVRKAEVEARRADLNMRRAQA 120 ++DNQLV GDLL+ ID + +A A+A + ++DL KA E A Sbjct: 74 IKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLA--KANNE------------A 119 Query: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQYQAALSQRDTAKLNLER 180 RR + +S E + A V A QA ++AAQA L+ QAQ+Q L + Sbjct: 120 NRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLK--QAQWQ------------LAQ 165 Query: 181 TKVFSPVDGYITNLNVRRGDYATAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDHAKIQ 240 T++ +PV G++TNL R GDYA G A++DSHSF+V YFEETKL + G A+I Sbjct: 166 TEIRAPVSGWVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQIT 225 Query: 241 LI-NGATMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIHLDKVPENV 299 L + T++GHV SI R IYD+ L DV P WVRLAQR+PVR LDKVP +V Sbjct: 226 LYSDNKTLQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDV 285 Query: 300 LLASGLTCTVVL 311 L SG TC++ + Sbjct: 286 TLVSGTTCSIAV 297 >Z2659 [V] COG1566 Multidrug resistance efflux pump EcZ Escherichia coli O157:H7 EDL933 Length = 299 Score = 235 bits (599), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 133/312 (42%), Positives = 184/312 (58%), Gaps = 30/312 (9%) Query: 1 MPVKSIFRVLITLVIFLVAILLAWSLWQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALP 60 M +K+I + T+++ +VA+L W LW +YM SPWTRDG++RA+ V+I P VSG++V L Sbjct: 15 MSIKTI-KYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQVSITPQVSGRIVELN 73 Query: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKADLEVRKAEVEARRADLNMRRAQA 120 ++DNQLV GDLL+ ID + +A A+A + ++DL KA E A Sbjct: 74 IKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLA--KANNE------------A 119 Query: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQYQAALSQRDTAKLNLER 180 RR + +S E + A V A QA ++AAQA L+ Q Q+Q L + Sbjct: 120 NRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLK--QTQWQ------------LAQ 165 Query: 181 TKVFSPVDGYITNLNVRRGDYATAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDHAKIQ 240 T++ +PV G++TNL R GDYA G A++DSHSF+V YFEETKL + G A+I Sbjct: 166 TEIRAPVSGWVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQIT 225 Query: 241 LI-NGATMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIHLDKVPENV 299 L + T++GHV SI R IYD+ L DV P WVRLAQR+PVR LDKVP +V Sbjct: 226 LYSDNKTLQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDV 285 Query: 300 LLASGLTCTVVL 311 L SG TC++ + Sbjct: 286 TLVSGTTCSIAV 297 >ECs2353 [V] COG1566 Multidrug resistance efflux pump Ecs Escherichia coli O157:H7 Length = 299 Score = 235 bits (599), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 133/312 (42%), Positives = 184/312 (58%), Gaps = 30/312 (9%) Query: 1 MPVKSIFRVLITLVIFLVAILLAWSLWQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALP 60 M +K+I + T+++ +VA+L W LW +YM SPWTRDG++RA+ V+I P VSG++V L Sbjct: 15 MSIKTI-KYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQVSITPQVSGRIVELN 73 Query: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKADLEVRKAEVEARRADLNMRRAQA 120 ++DNQLV GDLL+ ID + +A A+A + ++DL KA E A Sbjct: 74 IKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLA--KANNE------------A 119 Query: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQYQAALSQRDTAKLNLER 180 RR + +S E + A V A QA ++AAQA L+ Q Q+Q L + Sbjct: 120 NRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLK--QTQWQ------------LAQ 165 Query: 181 TKVFSPVDGYITNLNVRRGDYATAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDHAKIQ 240 T++ +PV G++TNL R GDYA G A++DSHSF+V YFEETKL + G A+I Sbjct: 166 TEIRAPVSGWVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQIT 225 Query: 241 LI-NGATMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIHLDKVPENV 299 L + T++GHV SI R IYD+ L DV P WVRLAQR+PVR LDKVP +V Sbjct: 226 LYSDNKTLQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDV 285 Query: 300 LLASGLTCTVVL 311 L SG TC++ + Sbjct: 286 TLVSGTTCSIAV 297