BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1776.p (214 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0906 [R] COG2872 Predicted metal-dependent hydrolases related... 80 3e-15 TM1726 [R] COG2872 Predicted metal-dependent hydrolases related ... 71 1e-12 BH2934 [R] COG2872 Predicted metal-dependent hydrolases related ... 65 1e-10 YNL040w [R] COG2872 Predicted metal-dependent hydrolases related... 62 8e-10 PH1969 [R] COG2872 Predicted metal-dependent hydrolases related ... 62 9e-10 >CAC0906 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Cac Clostridium acetobutylicum Length = 387 Score = 80.1 bits (196), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 57/227 (25%), Positives = 92/227 (40%), Gaps = 21/227 (9%) Query: 1 MTERLYLTQPALCADIEILSCAPLEDGRHAVRLSATPFHPQGGGQPSDVGWLGEVEVLRV 60 MT +L+ P +C + DG++ + L T F+P+GGGQP D+G + + V V Sbjct: 1 MTRKLFYDDPYICESEGKIVDVLENDGKYEIVLDETSFYPEGGGQPCDLGTINNIPVEYV 60 Query: 61 VSEGDDILHYTNAAVAPGPAVARVDAQPRQLHTRLHSAGHVIGHVMAQLGWKPTKAHHWP 120 +GD I H A +VD RQ + + H+ H++ +L H Sbjct: 61 YEKGDTIYHVMKERPEGNTAKCKVDFLRRQDNIQQHTGEHLLSAAFFKLYNGVNYGFHM- 119 Query: 121 GEGRVVGVSVGVGGEAAQELEAEEIERRCNALLAQD-----------------LACVLSL 163 GE V++ + E ++IE NA + + L + Sbjct: 120 GEDY---VTIDIDMEEVTNDMIKKIEAEANAYIYANVETKTYFMSKPEAEKLPLRKAIKA 176 Query: 164 EAGLRKIGFGDLPAYPCGGTHVATLGEIGKIGIESVRVKKGEVSIRY 210 E +R + GD+ C GTHV GE+G I + KG + + Sbjct: 177 EGKIRIVQLGDVDYSACCGTHVVRTGEVGIIKVLRTEKYKGMTRVYF 223 >TM1726 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Tma Thermotoga maritima Length = 373 Score = 71.2 bits (173), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 61/199 (30%), Positives = 94/199 (47%), Gaps = 22/199 (11%) Query: 24 LEDGRHAVRLSAT-PFHPQG-GGQPSDVGWLGEVEVLRVVSEGDDILHYTNAAVAPGPAV 81 ++DG + +S T PF+P G GGQ D G +G VL+V + ILHY +A + PG Sbjct: 12 IKDGEKIIAVSRTSPFYPDGKGGQLGDRGKIGPANVLKVKEKNGYILHYLDAHLEPGDYE 71 Query: 82 ARVDAQPRQLHTRLHSAGHVIGHVMAQLGWKPTKAHHWPGEGRVVGVSVGVGG------E 135 +D R+ H+A H++ ++ T + H GE RV + + E Sbjct: 72 CEIDPARRKDIACQHTAQHILSAAFLKVADLETVSFHM-GE-RVSTIDLNAPFVLEDVLE 129 Query: 136 AAQELEAEEIERRCNAL----------LAQDLACVLSLEAGLRKIGFGDLPAYPCGGTHV 185 A++L A E+ R C + +L + ++E +R + GD CGG HV Sbjct: 130 EAEDL-ANEVVRSCERVEILEIEKEEAEKMNLRKLPNVEGKVRVVKIGDFDVTACGGFHV 188 Query: 186 ATLGEIGKIGI-ESVRVKK 203 GEIG I I ++ +VKK Sbjct: 189 ENTGEIGLIKIVDTEKVKK 207 >BH2934 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Bha Bacillus halodurans Length = 242 Score = 64.7 bits (156), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 57/242 (23%), Positives = 105/242 (43%), Gaps = 38/242 (15%) Query: 1 MTERLYLT---QPALCADIEILSCAPLEDGRHAVRLSATPFHPQGGGQPSDVGWLGE--- 54 M++ LYL + +++++L+ + V L T FHP GGGQP+D GWL + Sbjct: 1 MSKELYLEDSYRQDYTSNVKLLN-------KDKVILEETIFHPNGGGQPNDEGWLYQNGK 53 Query: 55 -VEVLRVVSEGDDILHYTNAAVAPGPAVAR--VDAQPRQLHTRLHSAGHVI-GHVMAQLG 110 +V+ V EG++I+HY A R ++ R + H+ H++ GH+ + G Sbjct: 54 AYKVINVRKEGEEIIHYLEDADDLEEDEVRMKINWSRRYNFMKYHTLLHLVAGHLYHEYG 113 Query: 111 WKPTKAHHWPGEGRV----VGVSVGVGGEAAQE-----------LEAEEIERRCNALLAQ 155 + R+ G + V +E + +ER + +++ Sbjct: 114 ALAIGNQIFENRARLDIDFRGGELDVDFHRVEEELNRLINEGRKMNIRNVERDSES-ISE 172 Query: 156 DLACVLSL----EAGLRKIGFGDLPAYPCGGTHVATLGEIGKIGIESVRVK-KGEVSIRY 210 + V++L +R + ++ PC GTHV + EIG I + K KG+ + + Sbjct: 173 KIRTVINLIPASVKNIRLVAIENMDEQPCSGTHVNNINEIGPFKISGNKSKGKGKRRLEF 232 Query: 211 DV 212 + Sbjct: 233 QL 234 >YNL040w [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Sce Saccharomyces cerevisiae Length = 456 Score = 62.4 bits (150), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 59/230 (25%), Positives = 95/230 (41%), Gaps = 49/230 (21%) Query: 18 ILSCAPLEDGR-----HAVRLSATPFHPQGGGQPSDVGWLGEVEVLRVVSEGDDIL---- 68 ++SC P ++ + + + L T P+GGGQPSD G+L VE R S+ + IL Sbjct: 29 VVSCEPTKNKKGEIEGYEIELQDTILFPEGGGQPSDSGFLKIVEGNRNSSKIEKILVSHV 88 Query: 69 --------HYTNAAVAPGPAV-ARVDAQPRQLHTRLHSAGHVIGHVMAQ------LGW-- 111 H+ N + PG V VD Q R + + H+ H++ ++ + + W Sbjct: 89 SRFGLHAKHHVNDYIEPGTTVEVAVDEQKRMDYMQQHTGQHLLSAILERNYKVDTVSWSM 148 Query: 112 -----------KPTKAHHWPGEGR------VVGVSVGVGG---EAAQELEAEEI--ERRC 149 +P+ ++ R + VS + QE+ EE E Sbjct: 149 GGIITKKKPVLEPSDYFNYIELNRKLTLDEITNVSDEINQLIINFPQEIIVEERIGEETV 208 Query: 150 NALLAQDLACVLSLEAG-LRKIGFGDLPAYPCGGTHVATLGEIGKIGIES 198 + + + L G LR I GD+ + PC GTH+ +IG I I S Sbjct: 209 DEVSTSKIPDDYDLSKGVLRTIHIGDIDSNPCCGTHLKCTSQIGSILILS 258 >PH1969 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Pho Pyrococcus horikoshii Length = 404 Score = 62.0 bits (149), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 57/224 (25%), Positives = 94/224 (41%), Gaps = 34/224 (15%) Query: 1 MTERLYLTQPALCA-DIEILSCAPLEDGRHAVRLSATPFHPQGGGQPSDVGWLG----EV 55 M ER+Y P L D +I+ +D R V L T F+P+GGGQP D G + E+ Sbjct: 1 MVERVYYKDPYLREIDAKIVDVRKEKD-RVEVVLDRTIFYPEGGGQPGDRGVIKGNDFEI 59 Query: 56 EVLRVVSEGDDILH---YTNAAVAPGPAVA-RVDAQPRQLHTRLHSAGHVIGHVMAQLGW 111 V + + +I H G V +D + R + ++H+ H++ V+ +L Sbjct: 60 MVEDTIEKNGEIFHIGKLRGEIPKEGEKVKLYLDWEWRYGNMQMHTGQHILSAVLKKLYD 119 Query: 112 KPTKAHHWPGEGRVVGVSVGVGGEAAQELEAEEIERRCNALLAQDLACVL---------- 161 T + + + V GE ++ E E N ++ DL V+ Sbjct: 120 LDTTGFNIFRDY----AKIEVNGEVNWDM-IERAELEVNKIIINDLPVVIEEYDKLPEEI 174 Query: 162 ---------SLEAGLRKIGFGDLPAYPCGGTHVATLGEIGKIGI 196 ++ +R + GD+ PCGGTHV + E+G I + Sbjct: 175 ALKLRKNVTKVKEKIRIVKIGDVDVTPCGGTHVKSTREVGIIKV 218