BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1741.p (219 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5249 [E] COG1280 Putative threonine efflux protein Pae Pseudom... 154 1e-37 ECs4754 [E] COG1280 Putative threonine efflux protein Ecs Escher... 154 2e-37 VC0136 [E] COG1280 Putative threonine efflux protein Vch Vibrio ... 147 2e-35 STM3960 [E] COG1280 Putative threonine efflux protein Sty Salmon... 142 8e-34 YPO3831 [E] COG1280 Putative threonine efflux protein Ype Yersin... 136 4e-32 >PA5249 [E] COG1280 Putative threonine efflux protein Pae Pseudomonas aeruginosa Length = 209 Score = 154 bits (390), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 89/216 (41%), Positives = 122/216 (56%), Gaps = 10/216 (4%) Query: 1 MAYQTWITFFFAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEVALVVVLVIAGAG 60 M TW FF A I++SPG+GA+ SMS GL YG R LGL++ L + + I AG Sbjct: 1 MLVSTWFAFFLACWAISLSPGAGAIASMSCGLQYGFARGYWNALGLQIGLALQIAIVAAG 60 Query: 61 VGSLLVASEPAFNAVKIAGALYLIYLGFSQWRAPVPTEQEGEAIDAANAVARAQRGQAWW 120 VG+LL S AF+ +K G YL+YL QW+AP + + D + R Sbjct: 61 VGALLATSALAFSLIKWFGVAYLVYLAVRQWQAP----PQALSTDGERPLGRPL------ 110 Query: 121 QRCITGFLTNATNPKGIVFMVAVLPQFIDHERPLGLQLLVLGLTMVAVDVVVMHGYAFAA 180 + GFL NA+NPK ++FM+AVLPQFID +PL Q L++G TM+ VD++VM GY A Sbjct: 111 TLVLRGFLVNASNPKAVIFMLAVLPQFIDPHQPLLAQYLIMGGTMIVVDLIVMAGYTGLA 170 Query: 181 SRLQRFFRSQKALKAQNRFFGGVLMLVGAGLFFVKR 216 +R+ R RS + K NR F + + L V+R Sbjct: 171 ARVLRVLRSPRQQKLVNRTFASLFVGAAGLLATVRR 206 >ECs4754 [E] COG1280 Putative threonine efflux protein Ecs Escherichia coli O157:H7 Length = 206 Score = 154 bits (388), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 85/209 (40%), Positives = 126/209 (60%), Gaps = 11/209 (5%) Query: 1 MAYQTWITFFFAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEVALVVVLVIAGAG 60 M + W + + ++++SPGSGA+ +M+ LN+G R A+I GL+ L + +V+ G G Sbjct: 1 MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVG 60 Query: 61 VGSLLVASEPAFNAVKIAGALYLIYLGFSQWRAPVPTEQEGEAIDAANAVARAQRGQAWW 120 +G+L S AF +K AGA YLI+LG QWRA AID ++A Q + + Sbjct: 61 LGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRA-------AGAID-LKSLASTQSRRHLF 112 Query: 121 QRCITGFLTNATNPKGIVFMVAVLPQFIDHERPLGLQLLVLGLTMVAVDVVVMHGYAFAA 180 QR + N TNPK IVF+ A+ PQFI ++P +Q +VLG+T + VD++VM GYA A Sbjct: 113 QRAV---FVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDIIVMIGYATLA 169 Query: 181 SRLQRFFRSQKALKAQNRFFGGVLMLVGA 209 R+ + + K +KA N+ FG + MLVGA Sbjct: 170 QRIALWIKGPKQMKALNKIFGSLFMLVGA 198 >VC0136 [E] COG1280 Putative threonine efflux protein Vch Vibrio cholerae Length = 205 Score = 147 bits (371), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 81/215 (37%), Positives = 118/215 (54%), Gaps = 17/215 (7%) Query: 1 MAYQTWITFFFAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEVALVVVLVIAGAG 60 M W+ + A V +++PGSG V S+S+GL+YG R + I+GL++ L +V+ G G Sbjct: 1 MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIG 60 Query: 61 VGSLLVASEPAFNAVKIAGALYLIYLGFSQW--RAPVPTEQEGEAIDAANAVARAQRGQA 118 +G+L+ S AF +K GA YL++LG +W RAP+ + A + +A Sbjct: 61 IGALVAQSALAFTLIKWIGAAYLVWLGIQKWRDRAPLTATTTSHELSQAALLRKA----- 115 Query: 119 WWQRCITGFLTNATNPKGIVFMVAVLPQFIDHERPLGLQLLVLGLTMVAVDVVVMHGYAF 178 L N TNPK IVF+VA+ PQFID R Q LVLG+T V +D +VM GY Sbjct: 116 --------VLINLTNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAIVMFGYTA 167 Query: 179 AASRLQRFFRSQKALKAQNRFFGGVLMLVGAGLFF 213 A++L R+ RS + N+ FG M +G G+ Sbjct: 168 LAAQLGRYIRSPNIMTRMNKLFGS--MFMGCGMLL 200 >STM3960 [E] COG1280 Putative threonine efflux protein Sty Salmonella typhimurium LT2 Length = 206 Score = 142 bits (357), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 85/209 (40%), Positives = 124/209 (59%), Gaps = 11/209 (5%) Query: 1 MAYQTWITFFFAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEVALVVVLVIAGAG 60 M ++ W + + ++++SPGSGA+ +M+ +N+G R A+I GL+ L + +V+ G G Sbjct: 1 MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAVASIAGLQTGLGIHIVLVGVG 60 Query: 61 VGSLLVASEPAFNAVKIAGALYLIYLGFSQWRAPVPTEQEGEAIDAANAVARAQRGQAWW 120 +G+L S AF +K AGA YLI+LG QWRA AID RG+ + Sbjct: 61 LGTLFSRSLLAFEILKWAGAAYLIWLGIQQWRA-------AGAIDLHTLAQTQSRGRL-F 112 Query: 121 QRCITGFLTNATNPKGIVFMVAVLPQFIDHERPLGLQLLVLGLTMVAVDVVVMHGYAFAA 180 +R I N TNPK IVF+ A+ PQFI ++P Q L+LG+T + VD+VVM GYA A Sbjct: 113 KRAI---FVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMVVMTGYATLA 169 Query: 181 SRLQRFFRSQKALKAQNRFFGGVLMLVGA 209 R+ + + K +KA N+ FG + MLVGA Sbjct: 170 QRIAAWIKGPKQMKALNKAFGSLFMLVGA 198 >YPO3831 [E] COG1280 Putative threonine efflux protein Ype Yersinia pestis Length = 206 Score = 136 bits (342), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 83/209 (39%), Positives = 129/209 (61%), Gaps = 11/209 (5%) Query: 1 MAYQTWITFFFAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEVALVVVLVIAGAG 60 M W+T+ ++++SPGSGA+ +MS +++G R A+I GL++ L V +V+ G G Sbjct: 1 MTLDWWLTYLLTTLILSLSPGSGAINTMSTAISHGTRGVVASIGGLQLGLAVHIVLVGVG 60 Query: 61 VGSLLVASEPAFNAVKIAGALYLIYLGFSQWRAPVPTEQEGEAIDAANAVARAQRGQAWW 120 +G+L+ S AF +K GA YLI+LG QWRA ++D +A+A + + + Sbjct: 61 LGALVSQSLLAFEILKWLGAAYLIWLGIQQWRA-------AGSLDL-HALANSMPRRKLF 112 Query: 121 QRCITGFLTNATNPKGIVFMVAVLPQFIDHERPLGLQLLVLGLTMVAVDVVVMHGYAFAA 180 +R + N TNPK IVF+ A+ PQF+ ++P Q L+LG T V VD++VM GYA A Sbjct: 113 KRAV---FVNLTNPKSIVFLAALFPQFVLPQQPQVAQYLILGSTSVIVDIIVMIGYATLA 169 Query: 181 SRLQRFFRSQKALKAQNRFFGGVLMLVGA 209 +R+ R+ +S + +K NR FGG+ ML+GA Sbjct: 170 TRIARWIKSPQQMKLLNRIFGGLFMLIGA 198