BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1668.p (199 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2306 [E] COG1280 Putative threonine efflux protein Pae Pseudom... 163 2e-40 SMc01425 [E] COG1280 Putative threonine efflux protein Sme Sinor... 149 3e-36 mll1430 [E] COG1280 Putative threonine efflux protein Mlo Mesorh... 144 9e-35 VC1712 [E] COG1280 Putative threonine efflux protein Vch Vibrio ... 135 4e-32 YPO0181 [E] COG1280 Putative threonine efflux protein Ype Yersin... 109 3e-24 >PA2306 [E] COG1280 Putative threonine efflux protein Pae Pseudomonas aeruginosa Length = 205 Score = 163 bits (413), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 87/199 (43%), Positives = 123/199 (61%), Gaps = 7/199 (3%) Query: 2 IDALLPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHLLGITFGFGVLLAVCAFGIG 61 +D LL FAFV+S+TPGPNN ML +SG+ FGF RS+PH+LGI+ GF +++ FG+G Sbjct: 5 VDLLLAFSLFAFVTSVTPGPNNTMLLASGVNFGFVRSIPHILGISCGFFIMVMAVGFGLG 64 Query: 62 ELVVALPQVVLLLKALGCAYLLYLAWQLRHMAVA-QAAERALQPMSFWAAAMFQFANPKA 120 + A P + +L+ +G AYLLYLAW++ A E +P+S+W AA FQ+ NPKA Sbjct: 65 TVFEAYPVLYTILRYVGAAYLLYLAWKIATSGPAGNDPEGRGKPLSYWGAAAFQWVNPKA 124 Query: 121 WVMAITGASAFMPLLHEHAGRLAILIYCAVFCVVNLPCVSVWAGAGSLLRRYLEEPLWRV 180 WVMA+ S + PL +++ VF ++N P + +WAG GS+LR L +P W Sbjct: 125 WVMAVGAISTYTPLQGYFTN---VVVISTVFALINAPTIGIWAGFGSMLRNVLRDPRWLR 181 Query: 181 VFATTM---IVLTLYAALA 196 VF M +V +LY LA Sbjct: 182 VFNVGMALLLVASLYPILA 200 >SMc01425 [E] COG1280 Putative threonine efflux protein Sme Sinorhizobium meliloti Length = 197 Score = 149 bits (377), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 83/188 (44%), Positives = 116/188 (61%), Gaps = 3/188 (1%) Query: 2 IDALLPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHLLGITFGFGVLLAVCAFGIG 61 +D LL L FAF +SITPGPNN+ML +SG+ FGF R++PH+LGI GF VLL G+G Sbjct: 3 LDTLLALFLFAFTTSITPGPNNMMLFASGVNFGFVRTIPHMLGIGIGFFVLLIAVGLGLG 62 Query: 62 ELVVALPQVVLLLKALGCAYLLYLAWQLRHMAVAQAAERALQPMSFWAAAMFQFANPKAW 121 L+ ++P + LK G AYL+++AW++ PM+F AA FQ+ NPKAW Sbjct: 63 ALLHSVPLLYTALKFAGGAYLVWIAWKIGTSRSLGEGRANAVPMTFLQAAAFQWVNPKAW 122 Query: 122 VMAITGASAFMPLLHEHAGRLAILIYCAVFCVVNLPCVSVWAGAGSLLRRYLEEPLWRVV 181 VMA++ + + + +++L+ VF +VN+P VS WAG GS LR++L EP Sbjct: 123 VMAVSAMATYT---SSDSYLMSVLVVGLVFALVNVPSVSTWAGFGSALRQWLSEPARLKW 179 Query: 182 FATTMIVL 189 F TM VL Sbjct: 180 FNITMAVL 187 >mll1430 [E] COG1280 Putative threonine efflux protein Mlo Mesorhizobium loti Length = 198 Score = 144 bits (364), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 83/177 (46%), Positives = 114/177 (64%), Gaps = 4/177 (2%) Query: 2 IDALLPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHLLGITFGFGVLLAVCAFGIG 61 +DA L L+ +AFV+SITPGPNN+ML +SG+ FG R++PH+LGI+ GF VLL G+G Sbjct: 3 LDAFLALLVYAFVTSITPGPNNLMLLASGVNFGIARTVPHMLGISMGFLVLLLAVGLGLG 62 Query: 62 ELVVALPQVVLLLKALGCAYLLYLAWQLR-HMAVAQAAERALQPMSFWAAAMFQFANPKA 120 ++ A P + LK G AYLLYLAW++ ++ E +PM F AA FQ+ NPKA Sbjct: 63 AVLTAFPALHTALKIAGGAYLLYLAWKIAMSRSLTGKGETKARPMRFIDAAAFQWVNPKA 122 Query: 121 WVMAITGASAFMPLLHEHAGRLAILIYCAVFCVVNLPCVSVWAGAGSLLRRYLEEPL 177 WVMAIT + + H L++ + F +VNLP VSVWAG G+ LR +L +P+ Sbjct: 123 WVMAITAMAVYANAEHPF---LSVALISTAFTIVNLPSVSVWAGFGTALRGFLSDPV 176 >VC1712 [E] COG1280 Putative threonine efflux protein Vch Vibrio cholerae Length = 204 Score = 135 bits (341), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 75/170 (44%), Positives = 105/170 (61%), Gaps = 5/170 (2%) Query: 7 PLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHLLGITFGFGVLLAVCAFGIGELVVA 66 PL++FAFVS+ +PGPNNIML +SG GF R++PH+LGITFGF +++ + G+ EL Sbjct: 18 PLLAFAFVSTFSPGPNNIMLMTSGANVGFLRTIPHMLGITFGFSIMVLLVGVGLTELFQR 77 Query: 67 LPQVVLLLKALGCAYLLYLAWQLRHMAVAQAAERALQPMSFWAAAMFQFANPKAWVMAIT 126 P + L+ L YL+YLA ++ ++ + QPMSF AAA+FQ+ NPK W MA+T Sbjct: 78 YPILQQGLQILCTLYLVYLAVKIA-LSRPSKEGQEYQPMSFVAAALFQWVNPKGWSMALT 136 Query: 127 GASAFMPLLHEHAGRLAILIYCAVFCVVNLPCVSVWAGAGSLLRRYLEEP 176 S F P +A L + + VF +VNLP VS W AG L ++ P Sbjct: 137 AVSVFNP----NASWLQLGLIALVFALVNLPSVSAWTAAGKQLSYWMNHP 182 >YPO0181 [E] COG1280 Putative threonine efflux protein Ype Yersinia pestis Length = 200 Score = 109 bits (273), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 71/195 (36%), Positives = 108/195 (55%), Gaps = 4/195 (2%) Query: 6 LPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHLLGITFGFGVLLAVCAFGIGELVV 65 L ++ F +V++ITPGPNN++LTS+G FGF RS+ +LGI G +L + AFG+G L++ Sbjct: 7 LSMVGFLWVAAITPGPNNMLLTSTGANFGFMRSIWLMLGIMLGMQSMLLLVAFGVGSLIL 66 Query: 66 ALPQVVLLLKALGCAYLLYLAWQLRHMAVAQAAERA--LQPMSFWAAAMFQFANPKAWVM 123 P + L LK LG YLL+LAW++ A + +P+ + + QF NPKAW+M Sbjct: 67 IYPSLHLALKILGSLYLLWLAWKIATSAYEKLDTNVAPAKPLRLYQGWLLQFLNPKAWLM 126 Query: 124 AITGASAFMPLLHEHAGRLAILIYCAVFCVVNLPCVSVWAGAGSLLRRYLEEPLWRVVFA 183 + ++F + A +IL+ +VNL +W G G+L+ R L F Sbjct: 127 GLGSVASFS--MAGAAYNHSILMISLGMFMVNLVAGIIWLGFGTLIGRLLGSRRAWFTFN 184 Query: 184 TTMIVLTLYAALAIW 198 +M +LT L IW Sbjct: 185 ISMGLLTAACVLLIW 199