BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1312.p (359 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZyeaU [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase EcZ ... 565 e-161 ECs2509 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Ec... 565 e-161 yeaU [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Eco E... 565 e-161 RSp1612 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Rs... 562 e-160 YPO2496 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Yp... 557 e-158 >ZyeaU [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase EcZ Escherichia coli O157:H7 EDL933 Length = 361 Score = 565 bits (1457), Expect = e-161, Method: Compositional matrix adjust. Identities = 258/359 (71%), Positives = 308/359 (85%) Query: 1 MSKTHRIAVIAGDGIGQEVMPEGLRAVEAASKKFGIDIEFTHFDWAHCGYYAKHGQMMPD 60 M KT RIA I GDGIG+EV+PEG+R ++AA++++G + F +WA C YY+ HG+MMPD Sbjct: 1 MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPD 60 Query: 61 DWFEQLKGFEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 120 DW EQL F+AI+FGAVGWP TVPDH+SLWGSLLKFRR+FD YVNLRPVRL PGVPCPLA Sbjct: 61 DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA 120 Query: 121 NRKPGDIDFYVVRENTEGEYSAIGGRMFEGTERETVIQESVFSRKGTDRILKFAFDLAQS 180 ++PGDIDFYVVRENTEGEYS++GGR+ EGTE E VIQESVF+R+G DRIL++AF+LAQS Sbjct: 121 GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180 Query: 181 RPKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVV 240 RP+K LTSATKSNG+AI+MPYWDERVEAM YP++R DK HIDIL A FV+ P+RFDVV Sbjct: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240 Query: 241 VASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVAT 300 VASNLFGDILSDLGPAC GTIGIAPSAN+NP R PSLFEPVHGSAPDI+GKNIANP+AT Sbjct: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300 Query: 301 IWSGAMMLDFLGNGEGKYRQAHDAMLAAIEQVLVNGPTTPDLGGKSNTTEVGQAIAAAI 359 IW+GAMMLDFLGNG+ +++QAH+ +LAAIE+V+ +GP TPD+ G + T +V AI I Sbjct: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGSATTPQVADAICKII 359 >ECs2509 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Ecs Escherichia coli O157:H7 Length = 361 Score = 565 bits (1457), Expect = e-161, Method: Compositional matrix adjust. Identities = 258/359 (71%), Positives = 308/359 (85%) Query: 1 MSKTHRIAVIAGDGIGQEVMPEGLRAVEAASKKFGIDIEFTHFDWAHCGYYAKHGQMMPD 60 M KT RIA I GDGIG+EV+PEG+R ++AA++++G + F +WA C YY+ HG+MMPD Sbjct: 1 MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPD 60 Query: 61 DWFEQLKGFEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 120 DW EQL F+AI+FGAVGWP TVPDH+SLWGSLLKFRR+FD YVNLRPVRL PGVPCPLA Sbjct: 61 DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA 120 Query: 121 NRKPGDIDFYVVRENTEGEYSAIGGRMFEGTERETVIQESVFSRKGTDRILKFAFDLAQS 180 ++PGDIDFYVVRENTEGEYS++GGR+ EGTE E VIQESVF+R+G DRIL++AF+LAQS Sbjct: 121 GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180 Query: 181 RPKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVV 240 RP+K LTSATKSNG+AI+MPYWDERVEAM YP++R DK HIDIL A FV+ P+RFDVV Sbjct: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240 Query: 241 VASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVAT 300 VASNLFGDILSDLGPAC GTIGIAPSAN+NP R PSLFEPVHGSAPDI+GKNIANP+AT Sbjct: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300 Query: 301 IWSGAMMLDFLGNGEGKYRQAHDAMLAAIEQVLVNGPTTPDLGGKSNTTEVGQAIAAAI 359 IW+GAMMLDFLGNG+ +++QAH+ +LAAIE+V+ +GP TPD+ G + T +V AI I Sbjct: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGSATTPQVADAICKII 359 >yeaU [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Eco Escherichia coli K12 Length = 361 Score = 565 bits (1457), Expect = e-161, Method: Compositional matrix adjust. Identities = 258/359 (71%), Positives = 308/359 (85%) Query: 1 MSKTHRIAVIAGDGIGQEVMPEGLRAVEAASKKFGIDIEFTHFDWAHCGYYAKHGQMMPD 60 M KT RIA I GDGIG+EV+PEG+R ++AA++++G + F +WA C YY+ HG+MMPD Sbjct: 1 MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPD 60 Query: 61 DWFEQLKGFEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 120 DW EQL F+AI+FGAVGWP TVPDH+SLWGSLLKFRR+FD YVNLRPVRL PGVPCPLA Sbjct: 61 DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA 120 Query: 121 NRKPGDIDFYVVRENTEGEYSAIGGRMFEGTERETVIQESVFSRKGTDRILKFAFDLAQS 180 ++PGDIDFYVVRENTEGEYS++GGR+ EGTE E VIQESVF+R+G DRIL++AF+LAQS Sbjct: 121 GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180 Query: 181 RPKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVV 240 RP+K LTSATKSNG+AI+MPYWDERVEAM YP++R DK HIDIL A FV+ P+RFDVV Sbjct: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240 Query: 241 VASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVAT 300 VASNLFGDILSDLGPAC GTIGIAPSAN+NP R PSLFEPVHGSAPDI+GKNIANP+AT Sbjct: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300 Query: 301 IWSGAMMLDFLGNGEGKYRQAHDAMLAAIEQVLVNGPTTPDLGGKSNTTEVGQAIAAAI 359 IW+GAMMLDFLGNG+ +++QAH+ +LAAIE+V+ +GP TPD+ G + T +V AI I Sbjct: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKII 359 >RSp1612 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Rso Ralstonia solanacearum Length = 361 Score = 562 bits (1448), Expect = e-160, Method: Compositional matrix adjust. Identities = 259/357 (72%), Positives = 307/357 (85%) Query: 3 KTHRIAVIAGDGIGQEVMPEGLRAVEAASKKFGIDIEFTHFDWAHCGYYAKHGQMMPDDW 62 KT+RIAVI GDGIG+EVMPEGLR ++AA+++FGID+E+ H +WA C YY +HG+MMPDDW Sbjct: 4 KTYRIAVIPGDGIGREVMPEGLRVLDAAARRFGIDLEYQHIEWASCDYYLQHGRMMPDDW 63 Query: 63 FEQLKGFEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLANR 122 QL+G +AI FGAVGWPATVPDH+SLWGSLLKFRR+FD Y+NLRPVRL GVPCPLANR Sbjct: 64 KAQLQGADAILFGAVGWPATVPDHISLWGSLLKFRREFDQYINLRPVRLFEGVPCPLANR 123 Query: 123 KPGDIDFYVVRENTEGEYSAIGGRMFEGTERETVIQESVFSRKGTDRILKFAFDLAQSRP 182 KPGDID+YVVRENTEGEY+++GG M+EGT+RE VIQESV+SRKG +R+LKFAFDLA+SR Sbjct: 124 KPGDIDYYVVRENTEGEYTSLGGIMYEGTDREVVIQESVYSRKGAERLLKFAFDLARSRA 183 Query: 183 KKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVVVA 242 +KH+T ATKSNGIAI+MP+WD R + + YP+V DK HIDILTA FVL P RFDVV A Sbjct: 184 RKHVTLATKSNGIAISMPWWDARADEVATHYPEVTLDKQHIDILTARFVLQPGRFDVVAA 243 Query: 243 SNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVATIW 302 +NLFGDILSDLGPA GTIG+APSAN+NP R PSLFEPVHGSAPDI+GKNIANP+A IW Sbjct: 244 TNLFGDILSDLGPATTGTIGLAPSANLNPDRTFPSLFEPVHGSAPDIYGKNIANPIAMIW 303 Query: 303 SGAMMLDFLGNGEGKYRQAHDAMLAAIEQVLVNGPTTPDLGGKSNTTEVGQAIAAAI 359 S A+MLDFL G+G R AHDA++AAIE VL GP TPDLGG +NTT+VG+AIA+ + Sbjct: 304 SAALMLDFLTEGQGAGRAAHDAIVAAIEAVLKGGPRTPDLGGSANTTQVGEAIASHL 360 >YPO2496 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Ype Yersinia pestis Length = 365 Score = 557 bits (1435), Expect = e-158, Method: Compositional matrix adjust. Identities = 251/359 (69%), Positives = 301/359 (83%) Query: 1 MSKTHRIAVIAGDGIGQEVMPEGLRAVEAASKKFGIDIEFTHFDWAHCGYYAKHGQMMPD 60 M KT+RIA I GDGIG EVMPEG+ ++AA+ ++ +DI F + +WA C YY HGQMMP+ Sbjct: 5 MKKTYRIAAIPGDGIGHEVMPEGINVLQAAALRWDLDISFENIEWASCEYYQHHGQMMPN 64 Query: 61 DWFEQLKGFEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 120 DW QL+ F+AI+FGAVGWPATVPDH+SLWGSLLKFRRDF YVNLRPVRL PG+PCPL Sbjct: 65 DWQRQLQCFDAIYFGAVGWPATVPDHISLWGSLLKFRRDFQQYVNLRPVRLFPGIPCPLV 124 Query: 121 NRKPGDIDFYVVRENTEGEYSAIGGRMFEGTERETVIQESVFSRKGTDRILKFAFDLAQS 180 ++PGDIDFYVVRENTEGEYS++GGR+FEGTE+E V+Q S+F+R+G DRILK+AF+LAQ Sbjct: 125 GKQPGDIDFYVVRENTEGEYSSVGGRLFEGTEQEMVLQASIFTRRGVDRILKYAFELAQM 184 Query: 181 RPKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVV 240 RP+K LTSATKSNG+A +MPYWDER E M A+YP + DK HIDIL A FVLNP+RFDVV Sbjct: 185 RPRKRLTSATKSNGLAFSMPYWDERFEVMAAQYPQISADKQHIDILCARFVLNPERFDVV 244 Query: 241 VASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVAT 300 VASNLFGDILSDLGPACAGTIGIAPSAN+NP R+ PSLFEPVHGSAPDI+ +NIANP+A Sbjct: 245 VASNLFGDILSDLGPACAGTIGIAPSANLNPERDFPSLFEPVHGSAPDIYKRNIANPIAM 304 Query: 301 IWSGAMMLDFLGNGEGKYRQAHDAMLAAIEQVLVNGPTTPDLGGKSNTTEVGQAIAAAI 359 IW+GAMMLDFLG G+ +Y AH+ +L AIEQV+ GP TPD+ G ++T +VG AI + Sbjct: 305 IWAGAMMLDFLGQGDERYHAAHNGILNAIEQVIAQGPKTPDMCGDASTQQVGSAIVQVL 363