BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1297.p (492 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3504 [C] COG1012 NAD-dependent aldehyde dehydrogenases Pae Pse... 799 0.0 SMa2213 [C] COG1012 NAD-dependent aldehyde dehydrogenases Sme Si... 389 e-107 BH0539 [C] COG1012 NAD-dependent aldehyde dehydrogenases Bha Bac... 384 e-106 YER073w [C] COG1012 NAD-dependent aldehyde dehydrogenases Sce Sa... 374 e-103 BS_dhaS [C] COG1012 NAD-dependent aldehyde dehydrogenases Bsu Ba... 373 e-103 >PA3504 [C] COG1012 NAD-dependent aldehyde dehydrogenases Pae Pseudomonas aeruginosa Length = 494 Score = 799 bits (2063), Expect = 0.0, Method: Compositional matrix adjust. Identities = 394/492 (80%), Positives = 440/492 (89%) Query: 1 MMNLKEILPICIAGQWRLGSGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSS 60 M++L I PICIAG+WR G G+ Y + YPATGE VA LHAAS+ DVEEA+AGA AFR S Sbjct: 1 MIDLNPIQPICIAGEWRAGGGDLYESLYPATGEAVARLHAASLEDVEEAMAGAHRAFRES 60 Query: 61 GWAHKKAHERAAVLYRVAQLIRERSEALAQMQRLDNGKPISETRALVASAAATFQFFAAA 120 GWA +K HERA VLYR+A LIRERSE LAQ+QRLDNGKPI ETR LVASAAATFQFFAAA Sbjct: 61 GWAQRKPHERATVLYRIAALIRERSEELAQLQRLDNGKPIRETRNLVASAAATFQFFAAA 120 Query: 121 CETLEETLTPMRGDNLTMSLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAE 180 CETLEE++TP RGD ++MS+YEPMGVV AITPWNSPIASEAQK+APALAAGNAVVVKPAE Sbjct: 121 CETLEESITPSRGDFVSMSVYEPMGVVVAITPWNSPIASEAQKLAPALAAGNAVVVKPAE 180 Query: 181 VTPLAALELARICIEAGVPAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAH 240 +TPLAAL LARIC EAG+P G++SVLPGKG++IGDA+TRHPL +RVSFTGGT TGKHIA Sbjct: 181 ITPLAALALARICDEAGLPRGLVSVLPGKGALIGDALTRHPLARRVSFTGGTRTGKHIAR 240 Query: 241 IAADKMMPVSLELGGKSPTMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYD 300 IAADKMMPVSLELGGKSPTMV DAD+DHAVAGVLYGIFSSSGESCIAGSRLFVA YD Sbjct: 241 IAADKMMPVSLELGGKSPTMVLADADLDHAVAGVLYGIFSSSGESCIAGSRLFVASERYD 300 Query: 301 GFMERLAAAAAALRVGDPADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQG 360 FMERLA AAALRVGDPAD RTQMGPLI+ARHR+ +ERYVE+GVAEGGR+RTGG+RP G Sbjct: 301 EFMERLATGAAALRVGDPADERTQMGPLISARHRESVERYVEMGVAEGGRLRTGGLRPHG 360 Query: 361 AQFERGYYYTPTIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIW 420 F+RGY+YTPTIIEG++N R+C+EE+FGPVLVAMPF E+ LI +ANDS YALAAGIW Sbjct: 361 GAFDRGYFYTPTIIEGLTNGARLCREEVFGPVLVAMPFASEEALIEEANDSCYALAAGIW 420 Query: 421 TRDYKRAWRIGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMY 480 TRD++RAWR+GRAVQAG VWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQK++Y Sbjct: 421 TRDFQRAWRLGRAVQAGTVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKSLY 480 Query: 481 WGLNQQPLPWAG 492 WGLN+QPL WAG Sbjct: 481 WGLNEQPLAWAG 492 >SMa2213 [C] COG1012 NAD-dependent aldehyde dehydrogenases Sme Sinorhizobium meliloti Length = 494 Score = 389 bits (998), Expect = e-107, Method: Compositional matrix adjust. Identities = 201/481 (41%), Positives = 293/481 (60%), Gaps = 2/481 (0%) Query: 1 MMNLKEILPICIAGQWRL-GSGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRS 59 M NL + L + I GQW SGE T P T A + + D + A+ A AF Sbjct: 1 MQNLPK-LSMYIDGQWVAPASGEYIETVDPFTARPWALVPRGNAEDADRAVRAAHRAFSQ 59 Query: 60 SGWAHKKAHERAAVLYRVAQLIRERSEALAQMQRLDNGKPISETRALVASAAATFQFFAA 119 W ER ++ R A LI E ++ALA ++ DNG+ ++E + + ++A Sbjct: 60 GPWGKMHPTERGRIIQRFAALIEEHADALADIEVRDNGRLLAEMTHQIRYIPRWYHYYAG 119 Query: 120 ACETLEETLTPMRGDNLTMSLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPA 179 + +E TL P L+ S +EP+GV I PWN+P+ + K APALAAGN +V+KPA Sbjct: 120 FADKIEGTLHPCDKPALSFSRHEPLGVCVGIVPWNAPLLLFSLKAAPALAAGNTLVMKPA 179 Query: 180 EVTPLAALELARICIEAGVPAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIA 239 E T AL+L + +AG P G+I+V+ G G +G+ + HPL + V FTG T TG H+ Sbjct: 180 EFTSATALKLMELVEKAGFPTGVINVVTGYGPEVGEPLVTHPLTRHVGFTGSTKTGAHLY 239 Query: 240 HIAADKMMPVSLELGGKSPTMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLY 299 +AA + VSLELGGKSP +VF DAD+D+AV GV+ GIF + G++CIAGSRL V R+++ Sbjct: 240 SLAAKDVKRVSLELGGKSPNIVFGDADLDNAVRGVVGGIFGAVGQTCIAGSRLLVHRSIH 299 Query: 300 DGFMERLAAAAAALRVGDPADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQ 359 D F+E+LA R+GDP TQ+GP+ + + + Y+++ EG + GG RP Sbjct: 300 DEFLEKLAVFTKTARIGDPRKVETQIGPIANSMQFEKVLGYIDIARREGAELILGGGRPD 359 Query: 360 GAQFERGYYYTPTIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGI 419 + GY+ PTI GVSN RI +EE+FGPVL A+ FD+ ++ +A ANDS + L AG+ Sbjct: 360 LEECGTGYFIEPTIFAGVSNDMRIAREEVFGPVLSAIVFDEPEEALAIANDSEFGLGAGV 419 Query: 420 WTRDYKRAWRIGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAM 479 WT D + A ++ ++AG+VW+NTY+ S +TPFGG++ SG+GRE G GI +Y++ KA+ Sbjct: 420 WTSDMRLALKMSERLEAGSVWVNTYRDISYTTPFGGYKKSGIGRENGVAGIYEYLQTKAV 479 Query: 480 Y 480 + Sbjct: 480 W 480 >BH0539 [C] COG1012 NAD-dependent aldehyde dehydrogenases Bha Bacillus halodurans Length = 498 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 197/477 (41%), Positives = 287/477 (60%), Gaps = 6/477 (1%) Query: 10 ICIAGQWRLG-SGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAH 68 + I G+W SG+ + T PATGE +A + A D++ A+ A AF W+ A Sbjct: 26 LFINGEWVDSFSGQSFETMNPATGETLALVAEAGSEDIDFAVKAARKAFDEGPWSKMSAA 85 Query: 69 ERAAVLYRVAQLIRERSEALAQMQRLDNGKPISET-RALVASAAATFQFFAAACETLEET 127 ER+ ++Y++A LI + EALAQ++ LDNGKPI ET A + F++FA + Sbjct: 86 ERSRLIYKLADLIEDHKEALAQLETLDNGKPIRETANADIPLVIEHFRYFAGWATKIVGQ 145 Query: 128 LTPMRGDNLTMSLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAAL 187 P++G + +E +GVV I PWN P+ A K+ ALA G VV+KPAE TPL+AL Sbjct: 146 TIPVQGHYFNYTRHEAIGVVGQIIPWNFPLLMAAWKLGAALATGCTVVLKPAEQTPLSAL 205 Query: 188 ELARICIEAGVPAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMM 247 L + EAG P G+++++PG G G + HP V +V+FTG T GK I A++ + Sbjct: 206 YLGELINEAGFPKGVVNIVPGFGHSAGTPLVNHPAVDKVAFTGSTDVGKLIMKQASESLK 265 Query: 248 PVSLELGGKSPTMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLA 307 V+LELGGKSP ++ DAD+ AV G L GI + G+ C AGSRL+V + YD + L Sbjct: 266 RVTLELGGKSPNIILPDADLSKAVPGSLMGIMFNQGQVCCAGSRLYVQKKQYDHVVADLV 325 Query: 308 AAAAALRVGDPADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQGAQFERGY 367 + ++ GD +T MGPLI+ + + ++ Y++ GV EG + TGG P +++GY Sbjct: 326 SLTNEIQQGDGLLEQTTMGPLISNQQQSRVKNYIDRGVEEGAELLTGGNIP----YDQGY 381 Query: 368 YYTPTIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRA 427 + PTI V + I +EEIFGPV+ AMPFD DDLI +ANDS Y LAAG+WT+D K+A Sbjct: 382 FVAPTIFADVDHSMTIAKEEIFGPVVAAMPFDSIDDLIDKANDSDYGLAAGVWTQDIKKA 441 Query: 428 WRIGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMYWGLN 484 I ++AG +W+N Y F ++PFGG++ SG+GRE G + Y E K+++ ++ Sbjct: 442 HYIAHRIKAGTIWVNCYNVFDAASPFGGYKQSGIGREMGSYALENYTEVKSVWVNMD 498 >YER073w [C] COG1012 NAD-dependent aldehyde dehydrogenases Sce Saccharomyces cerevisiae Length = 520 Score = 374 bits (959), Expect = e-103, Method: Compositional matrix adjust. Identities = 184/457 (40%), Positives = 278/457 (60%), Gaps = 5/457 (1%) Query: 29 PATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHERAAVLYRVAQLIRERSEAL 88 P+ E + T++ A DV+EA+A A AF + W+ + RA L+ +A L+ + E L Sbjct: 66 PSNEEKITTVYKAMEDDVDEAVAAAKKAFETK-WSIVEPEVRAKALFNLADLVEKHQETL 124 Query: 89 AQMQRLDNGKPISETRALVASAAATFQFFAAACETLEETLTPMRGDNLTMSLYEPMGVVA 148 A ++ +DNGK + R VA + + + + + ++ T S+ EP+GV Sbjct: 125 AAIESMDNGKSLFCARGDVALVSKYLRSCGGWADKIYGNVIDTGKNHFTYSIKEPLGVCG 184 Query: 149 AITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELARICIEAGVPAGMISVLPG 208 I PWN P+ + K+ PALA GN VV+KPAE TPL+AL +++C EAG+PAG++++LPG Sbjct: 185 QIIPWNFPLLMWSWKIGPALATGNTVVLKPAETTPLSALFASQLCQEAGIPAGVVNILPG 244 Query: 209 KGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMMPVSLELGGKSPTMVFEDADVD 268 G V+G+ ++ HP VK+++FTG T TG+HI +AAD + V+LELGGKSP +VF DAD+D Sbjct: 245 SGRVVGERLSAHPDVKKIAFTGSTATGRHIMKVAADTVKKVTLELGGKSPNIVFADADLD 304 Query: 269 HAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLAAAAAALRVGDPADARTQMGPL 328 AV + +GIF +SGE C AGSR+++ T+Y+ +++L +L+VGDP D G Sbjct: 305 KAVKNIAFGIFYNSGEVCCAGSRIYIQDTVYEEVLQKLKDYTESLKVGDPFDEEVFQGAQ 364 Query: 329 ITARHRQGIERYVELGVAEGGRIRTGGVRPQGAQFERGYYYTPTIIEGVSNQQRICQEEI 388 + + I YV++ +EG R+ TGG R +GY+ PT+ V RI +EE+ Sbjct: 365 TSDKQLHKILDYVDVAKSEGARLVTGGAR----HGSKGYFVKPTVFADVKGDMRIVKEEV 420 Query: 389 FGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRAWRIGRAVQAGNVWINTYKQFS 448 FGP++ F D++IA ANDS Y LAAGI T D +A + + V+AG VWINTY F Sbjct: 421 FGPIVTVSKFSTVDEVIAMANDSQYGLAAGIHTNDINKAVDVSKRVKAGTVWINTYNNFH 480 Query: 449 ISTPFGGWRDSGLGREKGRLGILQYMEQKAMYWGLNQ 485 + PFGG+ SG+GRE G + Y + K++ +++ Sbjct: 481 QNVPFGGFGQSGIGREMGEAALSNYTQTKSVRIAIDK 517 >BS_dhaS [C] COG1012 NAD-dependent aldehyde dehydrogenases Bsu Bacillus subtilis Length = 495 Score = 373 bits (958), Expect = e-103, Method: Compositional matrix adjust. Identities = 190/465 (40%), Positives = 279/465 (60%), Gaps = 5/465 (1%) Query: 20 SGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHERAAVLYRVAQ 79 SG + T PATGE + TL+ A ADV++A+ A AF W R+ ++Y++A Sbjct: 33 SGATFDTPNPATGETLMTLYEAQAADVDKAVKAARKAFDQGEWRTMSPASRSRLMYKLAD 92 Query: 80 LIRERSEALAQMQRLDNGKPISET-RALVASAAATFQFFAAACETLEETLTPMRGDNLTM 138 L+ E LAQ++ LDNGKPI+ET + A +++A C + P+ G Sbjct: 93 LMEEHKTELAQLETLDNGKPINETTNGDIPLAIEHMRYYAGWCTKITGQTIPVSGAYFNY 152 Query: 139 SLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELARICIEAGV 198 + +EP+GVV I PWN P+ KM ALA G +V+KPAE TPL+AL LA + +AG Sbjct: 153 TRHEPVGVVGQIIPWNFPLLMAMWKMGAALATGCTIVLKPAEQTPLSALYLAELIDQAGF 212 Query: 199 PAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMMPVSLELGGKSP 258 PAG+I+++PG G G+A+T H V +++FTG T GK I AA + V+LELGGKSP Sbjct: 213 PAGVINIIPGFGEDAGEALTNHEAVDKIAFTGSTEIGKKIMSTAAKSIKRVTLELGGKSP 272 Query: 259 TMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLAAAAAALRVGDP 318 ++ DA++ A+ G L G+ + G+ C AGSR+F+ + YD ++ +A+ A +LR G Sbjct: 273 NILLPDANLKKAIPGALNGVMFNQGQVCCAGSRVFIHKDQYDEVVDEMASYAESLRQGAG 332 Query: 319 ADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQGAQFERGYYYTPTIIEGVS 378 TQ+GPL++ + + Y++ G EG + TGG P FE GY+ PT+ V Sbjct: 333 LHKDTQIGPLVSKEQHERVLSYIQKGKDEGAKAVTGGSCP----FEAGYFVAPTVFANVE 388 Query: 379 NQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRAWRIGRAVQAGN 438 ++ I +EEIFGPVL A+P++ D++I +AN S Y LAAG+WT + K+A I +QAG Sbjct: 389 DEMTIAKEEIFGPVLTAIPYETVDEVIERANHSEYGLAAGLWTENVKQAHYIADRLQAGT 448 Query: 439 VWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMYWGL 483 VW+N Y F ++PFGG++ SGLGRE G + Y E K+++ L Sbjct: 449 VWVNCYNVFDAASPFGGYKQSGLGREMGSYALDNYTEVKSVWVNL 493