BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1071.p (281 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1566 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 437 e-122 BMEI1708 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 395 e-110 mll4780 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 389 e-108 SMb21112 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 387 e-107 AGc22 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene... 379 e-105 >YPO1566 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Ype Yersinia pestis Length = 280 Score = 437 bits (1125), Expect = e-122, Method: Compositional matrix adjust. Identities = 205/281 (72%), Positives = 243/281 (86%), Gaps = 1/281 (0%) Query: 1 MKLLRYGPVGQEKPGILDQSGKIRDLSAYIKDVNGAVLDDASLDKIRKLDLESLPAVEGS 60 MKLLRYGPVG+E+P +LD+ G+IRDLSA+I+D++G L A+L K+R+LD SLP V G Sbjct: 1 MKLLRYGPVGEERPALLDELGEIRDLSAHIEDISGKFLLPATLYKLRQLDSASLPLVRGE 60 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDDVKIPRGSKK 120 R+GACVG++GKFIC+GLNYADHAAE+ IP EPV+FNKWTSA++GPNDD++IPR S K Sbjct: 61 QRLGACVGHVGKFICVGLNYADHAAETGAEIPQEPVIFNKWTSAIIGPNDDIEIPRNSVK 120 Query: 121 TDWEVELGVVIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVELGVVIG GG YI E +AMS+VAGYCVVND+SERE+Q+E GGTWDKGKGCDTFGP Sbjct: 121 TDWEVELGVVIGTGGRYIGEANAMSYVAGYCVVNDISEREFQLEHGGTWDKGKGCDTFGP 180 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFGVAHIVSYLSRFMSLQPGDVIS 240 IGPWLVTRDE+ DP +LG+WL+VDG+RYQNG+T TMIF + I+SYLSRFMSLQ GDVIS Sbjct: 181 IGPWLVTRDEIPDPHQLGLWLDVDGRRYQNGSTRTMIFKIPQIISYLSRFMSLQSGDVIS 240 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTMRLGIDGLGEQQQRTIDA 281 TGTPPGVG+G KP VYL AGQ MRLGI+GLGEQ Q+TI A Sbjct: 241 TGTPPGVGLGQKP-PVYLHAGQIMRLGIEGLGEQCQKTIQA 280 >BMEI1708 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Bme Brucella melitensis Length = 301 Score = 395 bits (1014), Expect = e-110, Method: Compositional matrix adjust. Identities = 181/276 (65%), Positives = 226/276 (81%), Gaps = 1/276 (0%) Query: 1 MKLLRYGPVGQEKPGILDQSGKIRDLSAYIKDVNGAVLDDASLDKIRKLDLESLPAVEGS 60 MK LRYG GQEKPG+LD G IRDLSA++ D++GA LD +L K+ LD+ SLP VEG+ Sbjct: 21 MKFLRYGAAGQEKPGLLDADGTIRDLSAHVSDLSGAALDPDALAKLGALDVNSLPKVEGN 80 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDDVKIPRGSKK 120 PRIG CV GKFICIGLNY+DHAAE+ +P+EP++F K TSA+VGPNDD+ IPRGS+K Sbjct: 81 PRIGPCVAGTGKFICIGLNYSDHAAETGAAVPSEPIIFMKATSAIVGPNDDLVIPRGSQK 140 Query: 121 TDWEVELGVVIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVELG++IGK Y+ E+DA+ +VAGYC ++DVSER +QIER G W KGK CDTFGP Sbjct: 141 TDWEVELGIIIGKTAKYVSEEDALDYVAGYCTIHDVSERAFQIERQGQWTKGKSCDTFGP 200 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFGVAHIVSYLSRFMSLQPGDVIS 240 GPWLVT+DE+ADP+ L +WL+V+G+ QNG++ TMI+GV H+VSYLS+FMSLQPGD+IS Sbjct: 201 TGPWLVTKDEIADPENLAVWLKVNGETMQNGSSRTMIYGVRHLVSYLSQFMSLQPGDIIS 260 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTMRLGIDGLGEQQQ 276 TGTPPGVGMG+KP YL+AG + LGI+GLG Q+Q Sbjct: 261 TGTPPGVGMGMKPPR-YLKAGDVVELGIEGLGSQKQ 295 >mll4780 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mlo Mesorhizobium loti Length = 281 Score = 389 bits (998), Expect = e-108, Method: Compositional matrix adjust. Identities = 182/276 (65%), Positives = 221/276 (80%), Gaps = 1/276 (0%) Query: 1 MKLLRYGPVGQEKPGILDQSGKIRDLSAYIKDVNGAVLDDASLDKIRKLDLESLPAVEGS 60 MKLLRYG VG E+PG+LD G IRDLSA++ D+ G L ASL+ + KLD +SLPAV G Sbjct: 1 MKLLRYGEVGSERPGLLDADGTIRDLSAHVADIAGTTLHPASLEMLGKLDPKSLPAVSGK 60 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDDVKIPRGSKK 120 PR+GACV GKFICIGLNY+DHAAE+ +P EP++F K +SA+VGP+DDV IPRGS+K Sbjct: 61 PRLGACVAGTGKFICIGLNYSDHAAETGATVPPEPIIFMKASSAIVGPDDDVLIPRGSEK 120 Query: 121 TDWEVELGVVIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVEL VVIGK Y+ E DA+ +VAGY V +DVSER +Q ER G W KGK CDTFGP Sbjct: 121 TDWEVELAVVIGKTAKYVSEADALDYVAGYAVAHDVSERAFQAERQGQWTKGKSCDTFGP 180 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFGVAHIVSYLSRFMSLQPGDVIS 240 GPWLVT+DEVADPQ L MWL V+GK QNG+T TM++GV ++VSYLS+FMSL PGD+IS Sbjct: 181 TGPWLVTKDEVADPQNLKMWLTVNGKTMQNGSTKTMVYGVKYLVSYLSQFMSLHPGDIIS 240 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTMRLGIDGLGEQQQ 276 TGTPPGVG+G+KP V+LRAG + LGI+GLG+Q+Q Sbjct: 241 TGTPPGVGLGMKP-PVFLRAGDVVELGIEGLGQQKQ 275 >SMb21112 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Sme Sinorhizobium meliloti Length = 281 Score = 387 bits (994), Expect = e-107, Method: Compositional matrix adjust. Identities = 178/277 (64%), Positives = 222/277 (80%), Gaps = 1/277 (0%) Query: 1 MKLLRYGPVGQEKPGILDQSGKIRDLSAYIKDVNGAVLDDASLDKIRKLDLESLPAVEGS 60 MKLLRYG GQEKPG+L G IRDLS ++ D+ LD + LD++ LD+E+LPAV G+ Sbjct: 1 MKLLRYGEPGQEKPGLLGSDGIIRDLSGHVSDLAAGALDPSKLDELANLDVETLPAVSGN 60 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDDVKIPRGSKK 120 PR+G CV GKFICIGLNY+DHAAE+ +P EP++F K TSA+VGPNDD+ +PRGS+K Sbjct: 61 PRLGPCVAGTGKFICIGLNYSDHAAETGATVPPEPIIFMKATSAIVGPNDDLVLPRGSEK 120 Query: 121 TDWEVELGVVIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVELG+VIGK Y+ E +A+ +VAGYC V+DVSER +Q ER G W KGK CDTFGP Sbjct: 121 TDWEVELGIVIGKTAKYVSEAEALDYVAGYCTVHDVSERAFQTERHGQWTKGKSCDTFGP 180 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFGVAHIVSYLSRFMSLQPGDVIS 240 GPWLVT+DEVADPQ L MWL+V+G+ Q+G+T TM++G AH+VSYLS+FMSL+PGD+IS Sbjct: 181 TGPWLVTKDEVADPQDLAMWLKVNGETMQDGSTKTMVYGAAHLVSYLSQFMSLRPGDIIS 240 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTMRLGIDGLGEQQQR 277 TGTPPGVGMG+KP YL+AG + LGI+GLG Q+QR Sbjct: 241 TGTPPGVGMGMKPPR-YLKAGDVVELGIEGLGSQKQR 276 >AGc22 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 280 Score = 379 bits (972), Expect = e-105, Method: Compositional matrix adjust. Identities = 179/279 (64%), Positives = 217/279 (77%), Gaps = 2/279 (0%) Query: 1 MKLLRYGPVGQEKPGILDQSGKIRDLSAYIKDVNGAVLDDASLDKIRKLDLESLPAVEGS 60 MKL+R G GQEKP ILD GK+RDLSA++KD+ G + L KI +DL +LP V Sbjct: 1 MKLMRVGQPGQEKPAILDAEGKVRDLSAHVKDIGGDAISPEGLKKIAAIDLATLP-VLSE 59 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDDVKIPRGSKK 120 RIGACV GKFICIGLN++DHAAE+ +P EPV+F K TSA+VGPND+V IPRGS+K Sbjct: 60 ERIGACVAGTGKFICIGLNFSDHAAETGATVPPEPVIFMKATSAIVGPNDNVTIPRGSEK 119 Query: 121 TDWEVELGVVIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVELGVVIGK Y+ E DA+ +VAGYCV +DVSER +Q ER G W KGK CDTFGP Sbjct: 120 TDWEVELGVVIGKTAKYVSEADALDYVAGYCVSHDVSERAFQTERAGQWTKGKSCDTFGP 179 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFGVAHIVSYLSRFMSLQPGDVIS 240 IGPWLVT+DE+ DPQ LGMWL+V+G+ Q+G++ TM++GVAH+VSYLS+FMSL PGDVIS Sbjct: 180 IGPWLVTKDEITDPQNLGMWLKVNGQTMQDGSSKTMVYGVAHVVSYLSQFMSLHPGDVIS 239 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTMRLGIDGLGEQQQRTI 279 TGTPPGVGMG KP YL+ G + LGI+GLG Q+Q + Sbjct: 240 TGTPPGVGMGQKPPR-YLKTGDVVELGIEGLGSQKQTFV 277