BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0981.p (358 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2451 [M] COG3264 Small-conductance mechanosensitive channel R... 253 5e-67 SMa1582 [M] COG3264 Small-conductance mechanosensitive channel S... 162 1e-39 AGc3014 [M] COG3264 Small-conductance mechanosensitive channel A... 126 8e-29 YPO3129 [M] COG3264 Small-conductance mechanosensitive channel Y... 117 5e-26 mll1272 [M] COG3264 Small-conductance mechanosensitive channel M... 115 1e-25 >RSc2451 [M] COG3264 Small-conductance mechanosensitive channel Rso Ralstonia solanacearum Length = 447 Score = 253 bits (646), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 145/334 (43%), Positives = 225/334 (67%), Gaps = 3/334 (0%) Query: 11 MRFVFYVLRGIFVKDATVGALLHLSEKLIALLVWLCVVLWITGLWPDFVDYLDSTTLPLA 70 + FVFYVLR + + + +L L E+++ L+W+ +VL++ G+ D V YL+ + Sbjct: 112 LYFVFYVLRRVLSANGDLHGMLVLVERVLTTLMWIGMVLYVVGVLSDVVSYLEGIQFSVG 171 Query: 71 -RHKVSLLTMLQGAASVLVTLIIALWIGTVLENRLMKFSGMHSSLRTVVARMMRALLILI 129 + KV+L ML +L+T+++A+W G+ L++R+ + + + ++L+ V++R+ +A L+L+ Sbjct: 172 GKQKVNLAAMLMAVVWILLTVLVAMWFGSWLDSRITRAAAIDANLKVVLSRVAKAALLLV 231 Query: 130 AFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDK 189 + L+SLSLVGIDLTVLSVFGGALGVG+G GLQKIAS+YVSGF+ILLERS+ +GD + V Sbjct: 232 SLLLSLSLVGIDLTVLSVFGGALGVGLGFGLQKIASNYVSGFIILLERSLKLGDQITVST 291 Query: 190 FFGKVTQINTRYTILEGLDGIETVLPNELFVSNPVQNYSLTHRIVRLSTQLTILYQDDVE 249 + G VTQI TRYT++ DG +T +PNEL V+ VQN++ VR++ ++ Y D E Sbjct: 292 YTGIVTQIRTRYTVVRNGDG-DTFVPNELMVAQAVQNHN-ERGTVRVAVRVQAAYPADPE 349 Query: 250 TVLSIMEQAALGVQRVSQQTAPQALLIKIGADGLELELGFWITDPENGRLNVLSEVNRAI 309 VL ++ A GV RV AP A L G+E ELG WI DP NG+L V S++NRAI Sbjct: 350 VVLGLLVACAEGVPRVLADPAPAAFLAAFADSGIEYELGVWIADPHNGKLGVQSDLNRAI 409 Query: 310 WAAFKRHGVQVAHPKRDIRIMDERSFRQSTAQEN 343 + FK GV++ +P+R++R++ + +QS+ ++ Sbjct: 410 YKRFKAAGVEIPYPQREVRLLGPLAVQQSSLSDS 443 >SMa1582 [M] COG3264 Small-conductance mechanosensitive channel Sme Sinorhizobium meliloti Length = 432 Score = 162 bits (410), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 100/293 (34%), Positives = 170/293 (58%), Gaps = 1/293 (0%) Query: 36 EKLIALLVWLCVVLWITGLWPDFVDYLDSTTLPLARHKVSLLTMLQGAASVLVTLIIALW 95 +L+A++ W + ++ TGL + LD+ + L ++SLL +L+ + + +A++ Sbjct: 126 SRLVAMVSWGYLAVYATGLDGPILSALDAAAVNLGVMRLSLLIVLKAVVLTVALIWVAVF 185 Query: 96 IGTVLENRLMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTVLSVFGGALGVG 155 +G VL + + + + S + +++++++ LI+IA ++LS GIDLT L+VF GA+GVG Sbjct: 186 VGNVLAHWVQRSGDLSPSFKVLISKVIKIALIMIAGAIALSATGIDLTALTVFSGAVGVG 245 Query: 156 IGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKFFGKVTQINTRYTILEGLDGIETVLP 215 +G GLQK+ S+++SG +ILL++S+ GD + + + FG + + R+ + DG E ++P Sbjct: 246 VGFGLQKVVSNFISGIIILLDKSIKPGDTITLGETFGSIRDLRARFVSVITRDGKEYLIP 305 Query: 216 NELFVSNPVQNYSLTHRIVRLSTQLTILYQDDVETVLSIMEQAALGVQR-VSQQTAPQAL 274 NE F+S V N+S + VR+ Y D V+ I + A V R V+ AP Sbjct: 306 NEDFISQQVVNWSFSSDYVRIDVDFGTSYDSDPHEVVRIAVETASAVPRVVNDYNAPVCW 365 Query: 275 LIKIGADGLELELGFWITDPENGRLNVLSEVNRAIWAAFKRHGVQVAHPKRDI 327 + GA L+ L FWI+DP NG NV +V A+W +FK GV + P R+I Sbjct: 366 MTAFGASSLDFRLRFWISDPANGLTNVRGQVLMALWDSFKEAGVSIPFPHREI 418 >AGc3014 [M] COG3264 Small-conductance mechanosensitive channel Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 881 Score = 126 bits (317), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 83/298 (27%), Positives = 150/298 (50%), Gaps = 4/298 (1%) Query: 38 LIALLVWLCVVLWITGLWPDFVD---YLDSTTLPLARHKVSLLTMLQGAASVLVTLIIAL 94 ++AL + ++L+ G P ++ Y T + + +SL+ + G + II Sbjct: 523 VVALAFGVPLILFSWGFQPGDIESWAYRLLTGITVGNASISLIGLFGGVLVFAIGYIITR 582 Query: 95 WIGTVLENRLMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTVLSVFGGALGV 154 W L+N +M + + +R V + L I +A + +S G++L+ L++ AL V Sbjct: 583 WFQKWLDNNVMARGQVDAGVRNSVKTGIGYLGIAVAAIFGVSSAGLNLSSLALVASALSV 642 Query: 155 GIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKFFGKVTQINTRYTILEGLDGIETVL 214 GIG GLQ I S++VSG ++L+ER +GD V G V +++ R T +E G ++ Sbjct: 643 GIGFGLQNIVSNFVSGLILLVERPFKVGDWVVTGTTEGTVKRLSVRATEIETFRGQSIIV 702 Query: 215 PNELFVSNPVQNYSLTHRIVRLSTQLTILYQDDVETVLSIMEQAALGVQRVSQQTAPQAL 274 PN F+++ V N++ +RI+R +++ Y D + V+ I+ + V + P Sbjct: 703 PNSEFINSSVGNWTHRNRIMRAEIPVSVAYDSDPQQVMDILLELVRAQPPVLRNPEPHVE 762 Query: 275 LIKIGADGLELELGFWITDPENGRLNVLSEVNRAIWAAFKRHGVQVAHPKRDIRIMDE 332 ++ G L+ EL F + D NG L V + + AI F+ G+ + P+R++ I E Sbjct: 763 FLRFGDFSLDFELRFHLADLSNG-LAVKNALRIAILHRFREEGIAIPFPQRNLNIHVE 819 >YPO3129 [M] COG3264 Small-conductance mechanosensitive channel Ype Yersinia pestis Length = 1139 Score = 117 bits (292), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 88/327 (26%), Positives = 155/327 (47%), Gaps = 36/327 (11%) Query: 41 LLVWLCVVLWITGLWPDFV---DYLDSTTL---------PLARHKVSLLTMLQGAASVLV 88 L++ WI W D + YLDS +L V+L ML +++V Sbjct: 808 FLIFATCFYWI---WADLITVISYLDSISLWHYTATVAGASVTQAVTLGNMLVALMALVV 864 Query: 89 TLIIALWIGTVLE----NRLMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTV 144 ++ + +LE +RL G ++ T++ ++ A + + +LS +G+ Sbjct: 865 AYVLTRNLPGLLEVVVLSRLQLRQGTSYAITTILTYLITA----VGGITALSSLGVSWDK 920 Query: 145 LSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKFFGKVTQINTRYTIL 204 L AL VG+G GLQ+I +++VSG +IL ER + IGD + + + G V++I R T + Sbjct: 921 LQWLVAALSVGLGFGLQEIFANFVSGLIILFERPVRIGDTITIGTYSGTVSRIRIRATTI 980 Query: 205 EGLDGIETVLPNELFVSNPVQNYSLTHRIVRLSTQLTILYQDDVETVLSIMEQAALGVQR 264 D E ++PN+ FV+ + N+SL+ I R+ ++ + Y D+E V ++ QAA R Sbjct: 981 TDFDRKEVIVPNKAFVTERLINWSLSDTITRIIIKVGVAYGSDLEKVKEVLLQAAHANSR 1040 Query: 265 VSQQTAPQALLIKIGADGLELELGFWITDPENGRLNVLSEVNRAIWAAFKRHGVQVAHPK 324 V PQ + G+ L+ EL ++ + + R + E+NRAI + + + +A + Sbjct: 1041 VMTDPEPQVFFLNFGSSTLDHELRLYVRELRD-RSYTVDELNRAIDKLCRENDINIAFNQ 1099 Query: 325 RDIRIMDERSFRQSTAQENPDGPGTQN 351 D+ + PDG Q Sbjct: 1100 LDVYL------------HKPDGTEVQE 1114 >mll1272 [M] COG3264 Small-conductance mechanosensitive channel Mlo Mesorhizobium loti Length = 850 Score = 115 bits (289), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 78/303 (25%), Positives = 151/303 (49%), Gaps = 4/303 (1%) Query: 28 VGALLHLSEKLIALLVWLCVVLWITGLWPDFVD---YLDSTTLPLARHKVSLLTMLQGAA 84 +G ++ ++ L+ +LV+L ++L + G P + Y +T + + +S+ +L G Sbjct: 499 LGLVVSVAINLMIVLVFLPLILLMWGFQPGDIQAWAYKLATGITIGSVTISVTGILSGII 558 Query: 85 SVLVTLIIALWIGTVLENRLMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTV 144 ++ + W L+ +M + + +R + + + +A LV +S GIDL+ Sbjct: 559 VFIIGYFLTRWFQGWLDGSVMARGKVDTGVRNSIRLAVGYAGVAVAGLVGISAAGIDLSS 618 Query: 145 LSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKFFGKVTQINTRYTIL 204 L++ GAL +GIG GLQ + S++VSG ++L ER +GD + G V +I+ R T + Sbjct: 619 LALVAGALSLGIGFGLQNVVSNFVSGLILLAERPFKVGDWIVAGDVSGTVKKISVRATEI 678 Query: 205 EGLDGIETVLPNELFVSNPVQNYSLTHRIVRLSTQLTILYQDDVETVLSIMEQAALGVQR 264 E +LPN ++N V N++ +++ R+ ++ + Y DV+ V +I+ + A Sbjct: 679 ETFQRQSVILPNSNLINNAVGNWTHRNKLGRIDIKVGVAYGSDVKQVHAILLEIARSHPM 738 Query: 265 VSQQTAPQALLIKIGADGLELELGFWITDPENGRLNVLSEVNRAIWAAFKRHGVQVAHPK 324 V + P L G LE E+ ++ D NG + V ++V A+ F +++ Sbjct: 739 VLKNPEPFVLFSNFGPAALEFEIRVFLADVTNGNI-VQNDVRFAVLEKFSDQHIEIPSTP 797 Query: 325 RDI 327 R + Sbjct: 798 RAV 800