BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0975.p (401 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0306 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 377 e-104 PA3241 [R] COG1752 Predicted esterase of the alpha-beta hydrolas... 319 7e-87 RSc0221 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 58 3e-08 Z1654 [R] COG1752 Predicted esterase of the alpha-beta hydrolase... 55 3e-07 Z1214 [R] COG1752 Predicted esterase of the alpha-beta hydrolase... 55 3e-07 >RSc0306 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Rso Ralstonia solanacearum Length = 391 Score = 377 bits (968), Expect = e-104, Method: Compositional matrix adjust. Identities = 198/390 (50%), Positives = 258/390 (66%), Gaps = 7/390 (1%) Query: 7 KTGLILTGGGARAAYQVGVLNAISMILLEAGWPVHSNPFPIICGTSAGAINGTALACRAD 66 T L+L GGG+RAAYQ GVL I+ + E +PF +ICGTSAGAING LA A Sbjct: 3 STALLLMGGGSRAAYQAGVLRGIARLARECAPDAVGSPFDVICGTSAGAINGVGLARGAQ 62 Query: 67 DFGEAVRELMAVWSNFKVEQVYRADSLGVIRSGARWLSLFSFGWLLRKWRANPPNSLLDN 126 DF +A L+ +W+ ++VYR D V SGARWL+ +FGW+ + P +L DN Sbjct: 63 DFLQATEALVQLWAKLHADRVYRTDVGRVGASGARWLTALAFGWMTGR----TPRALFDN 118 Query: 127 TPLVSMLHRLLDLPRLDAALEQGQLHALAVTASSYTSGQHITFYQCAEEIEPWRRTQRLA 186 TPL +L D +L AA + G L ALAVTA SYT+G+H+TFYQ + PWRR+QR+A Sbjct: 119 TPLRELLLEQHDAQQLQAAFDAGALRALAVTALSYTTGRHVTFYQSPHVVLPWRRSQRIA 178 Query: 187 MRDQIGIDHLLASSAIPFIFPAIPVYIDGRCEYCGDGSMRQIAPISPAIHLGATKVLIIG 246 + D IG+ HLLASS+IPF+FPA PV ++G E+ GDG+MRQ++P+SPAIHLGA+++L++G Sbjct: 179 VTDTIGVPHLLASSSIPFLFPAEPVQLEGNDEWFGDGTMRQMSPLSPAIHLGASRILVVG 238 Query: 247 AGRMGEKGEQAPTNTPHYPSLAQVAGHAMSGIFLDGLSADIERINRVNQTLSVLNEEQRR 306 A + G YPSLAQVAG A++ +FLDGLSADIER+ VN +S E Sbjct: 239 AASRQQPGWYDTVPASGYPSLAQVAGQALASVFLDGLSADIERLQHVNAMVSRNPEIADA 298 Query: 307 HTPLKPIRTLVISPSHRIDSIASRHVLNLPRPIRTMLGGIGATEARGSALASYLLFEQSF 366 + I LV+SPS RI+ IA+RHV LP +R +L +G TEARG+A ASYLLFE F Sbjct: 299 RDGWRKIEVLVMSPSERIELIAARHVQRLPGTVRALLRPLGGTEARGAAFASYLLFEPEF 358 Query: 367 TRELIALGQSDTYAKREEVLAFFE---PDT 393 TRELI LG+ D A+R+E+ AF PDT Sbjct: 359 TRELIDLGERDALARRDELAAFLYGVVPDT 388 >PA3241 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Pae Pseudomonas aeruginosa Length = 389 Score = 319 bits (818), Expect = 7e-87, Method: Compositional matrix adjust. Identities = 187/390 (47%), Positives = 244/390 (62%), Gaps = 22/390 (5%) Query: 8 TGLILTGGGARAAYQVGVLNAISMILLEAGWPVHSNPFPIICGTSAGAINGTALACRADD 67 TGLIL+GGGARAAYQVGVL AI+ +L ++ NPFP+I GTSAGAIN LAC A Sbjct: 12 TGLILSGGGARAAYQVGVLAAIADLLPDSA----GNPFPVIVGTSAGAINAVGLACGALQ 67 Query: 68 FGEAVRELMAVWSNFKVEQVYRADSLGVIRSGARWL--SLFSFGWLLRKWRANPPNSLLD 125 F +A++ L AVW F+ E VYR+D GV R +R+L SL G + P +LLD Sbjct: 68 FRQAIQRLTAVWQGFRTEHVYRSDWPGVTRQASRFLGHSLLGLGRRV-------PVALLD 120 Query: 126 NTPLVSMLHRLLDLPRLDAALEQGQLHALAVTASSYTSGQHITFYQCAEEIEPWRRTQRL 185 N PL +L R LD + AA+ Q QL A+AVTA Y SGQ +TFYQ I+PW R +RL Sbjct: 121 NRPLHELLARELDFSGIHAAVRQRQLRAVAVTAFGYESGQAVTFYQGRATIDPWVRHRRL 180 Query: 186 AMRDQIGIDHLLASSAIPFIFPAIPVYIDGRCEYCGDGSMRQIAPISPAIHLGATKVLII 245 + ++ + HLLAS+AIP +FP PV I+ EY GDG++R APISPA+HLGAT+VL+I Sbjct: 181 GIPTRLELRHLLASAAIPLLFP--PVRIN--REYFGDGAVRSQAPISPALHLGATRVLVI 236 Query: 246 G-AGRMGEKGEQAPTNTP---HYPSLAQVAGHAMSGIFLDGLSADIERINRVNQTLSVLN 301 G +G G+ A T P PSLAQ+ H ++ F+D L DIE++ R+NQ ++ Sbjct: 237 GVSGNPATGGDSAMTGLPPSDRPPSLAQIGAHLLNSTFIDSLEGDIEQLQRMNQIGQLVA 296 Query: 302 EEQRRHT-PLKPIRTLVISPSHRIDSIASRHVLNLPRPIRTMLGGIGATEARGSALASYL 360 E RR L+P+ L++SPS +D IA+RH L R +R L G GAT A G+ + SYL Sbjct: 297 PEVRRQAGGLEPVEVLLVSPSRPLDEIAARHYRELSRVLRLFLRGPGATRASGAGVLSYL 356 Query: 361 LFEQSFTRELIALGQSDTYAKREEVLAFFE 390 LFE+ + ELI LG D KR E+ F Sbjct: 357 LFERGYCSELIELGYHDAMGKRTELERFLR 386 >RSc0221 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Rso Ralstonia solanacearum Length = 303 Score = 58.2 bits (139), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 68/243 (27%), Positives = 98/243 (40%), Gaps = 57/243 (23%) Query: 7 KTGLILTGGGARAAYQVGVLNAISMILLEAGWPVHSNPFP-IICGTSAGAINGTALACRA 65 +T + GGG+ A + G+L + V P ++ G SAGAIN AC Sbjct: 6 RTAFVFAGGGSLGAIEAGMLREL----------VARGVMPDMVVGASAGAINAAFFACHP 55 Query: 66 DDFGEAVRELMAVWSNFKVEQVYRADSLGVIRSGARWLSLFSFGWLLRKWRANPPNSLLD 125 G A L A+W + +V W G L R+ L+D Sbjct: 56 HMDGAA--RLEALWRGVRRAEVMP------------WSWRVMLGMLGRR-----STHLVD 96 Query: 126 NTPLVSMLHRLLDLPRLDAALEQGQLHALAVTASSYTSGQHITFYQCAEEIEPWRRTQRL 185 L +L+R RL+AA L V A+ +G + + Sbjct: 97 AGGLQRLLNRHFAQARLEAAPLP-----LHVVATDMQTGAEVVLSTGSI----------- 140 Query: 186 AMRDQIGIDHLLASSAIPFIFPAIPVYIDGRCEYCGDGSMRQIAPISPAIHLGATKVLII 245 + +LAS+AIP +FP PV +DGR DG + PIS AI LGAT+V+++ Sbjct: 141 -------VQAVLASAAIPGVFP--PVRVDGRLLI--DGGVANNTPISTAIRLGATRVVVL 189 Query: 246 GAG 248 AG Sbjct: 190 PAG 192 >Z1654 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 356 Score = 54.7 bits (130), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 35/91 (38%), Positives = 49/91 (53%), Gaps = 10/91 (10%) Query: 3 TMEHKTGLILTGGGARAAYQVGVLNAISMILLEAGWPVHSNPFPIICGTSAGAINGTALA 62 + E KTGL+L+GGGA AYQ GV+ A++ E G + ++ GTS GA NG +A Sbjct: 2 STEMKTGLVLSGGGAVGAYQAGVVKALA----ECGTQI-----SMVSGTSIGAFNGAIIA 52 Query: 63 CRADDFGEAVRELMAVWSNFKVEQVYRADSL 93 + D EA L A+W + QV + L Sbjct: 53 A-SPDLSEAAVRLEALWEHLGNNQVLSVNRL 82 >Z1214 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 356 Score = 54.7 bits (130), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 35/91 (38%), Positives = 49/91 (53%), Gaps = 10/91 (10%) Query: 3 TMEHKTGLILTGGGARAAYQVGVLNAISMILLEAGWPVHSNPFPIICGTSAGAINGTALA 62 + E KTGL+L+GGGA AYQ GV+ A++ E G + ++ GTS GA NG +A Sbjct: 2 STEMKTGLVLSGGGAVGAYQAGVVKALA----ECGTQI-----SMVSGTSIGAFNGAIIA 52 Query: 63 CRADDFGEAVRELMAVWSNFKVEQVYRADSL 93 + D EA L A+W + QV + L Sbjct: 53 A-SPDLSEAAVRLEALWEHLGNNQVLSVNRL 82