BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0960.p (459 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ygcX [MR] COG4948 L-alanine-DL-glutamate epimerase and related e... 708 0.0 ECs3647 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 708 0.0 ZygcX [MR] COG4948 L-alanine-DL-glutamate epimerase and related ... 707 0.0 STM2960 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 705 0.0 BS_ycbF [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 703 0.0 >ygcX [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Eco Escherichia coli K12 Length = 446 Score = 708 bits (1827), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/440 (75%), Positives = 381/440 (86%), Gaps = 1/440 (0%) Query: 21 APRVTEMRVIPVAGHDSMLLNLSGAHGPYFTRNIVILTDSAGHTGVGEVPGGEGIRQTLE 80 P VTEM+VIPVAGHDSML+NLSGAH P+FTRNIVI+ D++GHTGVGE+PGGE IR+TLE Sbjct: 7 TPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIKDNSGHTGVGEIPGGEKIRKTLE 66 Query: 81 DARALVVGQSIGNVQGILNRARSAFADRDAGGRGLQTFDLRIAIHAVTALEAALLDLLGQ 140 DA LVVG+++G + +L R+ FADRDAGGRGLQTFDLR IH VT +EAA+LDLLGQ Sbjct: 67 DAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQTFDLRTTIHVVTGIEAAMLDLLGQ 126 Query: 141 FLEVPVAALLGEGQQRSEVEMLGYLFYVGDRNKTDLPYLSAPDAADDWLRLRHEAAMTPE 200 L V VA+LLG+GQQRSEVEMLGYLF+VG+R T LPY S PD + DW RLRHE AMTP+ Sbjct: 127 HLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKATPLPYQSQPDDSCDWYRLRHEEAMTPD 186 Query: 201 AVVRLAEAAYARYGFNDFKLKGGVMRGEEEIVAVTALAERFPKARITLDPNGGWLLKDAI 260 AVVRLAEAAY +YGFNDFKLKGGV+ GEEE ++ ALA+RFP+ARITLDPNG W L +AI Sbjct: 187 AVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAESIVALAQRFPQARITLDPNGAWSLNEAI 246 Query: 261 RLCRDQHDVLAYAEDPCGAEEGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQS 320 ++ + LAYAEDPCGAE+G+SGREVMAEFRRATGL TATNM+ATDWRQMGH +SLQS Sbjct: 247 KIGKYLKGSLAYAEDPCGAEQGFSGREVMAEFRRATGLPTATNMIATDWRQMGHTLSLQS 306 Query: 321 VDIPLADPHFWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID 380 VDIPLADPHFWTMQGSVRVAQMC+E+GLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID Sbjct: 307 VDIPLADPHFWTMQGSVRVAQMCHEFGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID 366 Query: 381 THWIWQEG-QRLTREPLQIVGGKVQVPSKPGLGVEVDMAQIEAAHQTYRNMGLGARDDAV 439 THWIWQEG QRLT+EP +I GG VQVP KPGLGVE+DM Q+ AH+ Y+ GLGARDDA+ Sbjct: 367 THWIWQEGNQRLTKEPFEIKGGLVQVPEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAM 426 Query: 440 AMQYLIPGWKFDHKKPCLVR 459 MQYLIPGW FD+K+PC+VR Sbjct: 427 GMQYLIPGWTFDNKRPCMVR 446 >ECs3647 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Ecs Escherichia coli O157:H7 Length = 446 Score = 708 bits (1827), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/440 (75%), Positives = 381/440 (86%), Gaps = 1/440 (0%) Query: 21 APRVTEMRVIPVAGHDSMLLNLSGAHGPYFTRNIVILTDSAGHTGVGEVPGGEGIRQTLE 80 P VTEM+VIPVAGHDSML+NLSGAH P+FTRNIVI+ D++GHTGVGE+PGGE IR+TLE Sbjct: 7 TPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIKDNSGHTGVGEIPGGEKIRKTLE 66 Query: 81 DARALVVGQSIGNVQGILNRARSAFADRDAGGRGLQTFDLRIAIHAVTALEAALLDLLGQ 140 DA LVVG+++G + +L R+ FADRDAGGRGLQTFDLR IH VT +EAA+LDLLGQ Sbjct: 67 DAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQTFDLRTTIHVVTGIEAAMLDLLGQ 126 Query: 141 FLEVPVAALLGEGQQRSEVEMLGYLFYVGDRNKTDLPYLSAPDAADDWLRLRHEAAMTPE 200 L V VA+LLG+GQQRSEVEMLGYLF+VG+R T LPY S PD + DW RLRHE AMTP+ Sbjct: 127 HLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKATPLPYQSQPDDSCDWYRLRHEEAMTPD 186 Query: 201 AVVRLAEAAYARYGFNDFKLKGGVMRGEEEIVAVTALAERFPKARITLDPNGGWLLKDAI 260 AVVRLAEAAY +YGFNDFKLKGGV+ GEEE ++ ALA+RFP+ARITLDPNG W L +AI Sbjct: 187 AVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAESIVALAQRFPQARITLDPNGAWSLNEAI 246 Query: 261 RLCRDQHDVLAYAEDPCGAEEGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQS 320 ++ + LAYAEDPCGAE+G+SGREVMAEFRRATGL TATNM+ATDWRQMGH +SLQS Sbjct: 247 KIGKYLKGSLAYAEDPCGAEQGFSGREVMAEFRRATGLPTATNMIATDWRQMGHTLSLQS 306 Query: 321 VDIPLADPHFWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID 380 VDIPLADPHFWTMQGSVRVAQMC+E+GLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID Sbjct: 307 VDIPLADPHFWTMQGSVRVAQMCHEFGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID 366 Query: 381 THWIWQEG-QRLTREPLQIVGGKVQVPSKPGLGVEVDMAQIEAAHQTYRNMGLGARDDAV 439 THWIWQEG QRLT+EP +I GG VQVP KPGLGVE+DM Q+ AH+ Y+ GLGARDDA+ Sbjct: 367 THWIWQEGNQRLTKEPFEIKGGLVQVPEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAM 426 Query: 440 AMQYLIPGWKFDHKKPCLVR 459 MQYLIPGW FD+K+PC+VR Sbjct: 427 GMQYLIPGWTFDNKRPCMVR 446 >ZygcX [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 446 Score = 707 bits (1826), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/440 (75%), Positives = 381/440 (86%), Gaps = 1/440 (0%) Query: 21 APRVTEMRVIPVAGHDSMLLNLSGAHGPYFTRNIVILTDSAGHTGVGEVPGGEGIRQTLE 80 P VTEM+VIPVAGHDSML+NLSGAH P+FTRNIVI+ D++GHTGVGE+PGGE IR+TLE Sbjct: 7 TPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIKDNSGHTGVGEIPGGEKIRKTLE 66 Query: 81 DARALVVGQSIGNVQGILNRARSAFADRDAGGRGLQTFDLRIAIHAVTALEAALLDLLGQ 140 DA LVVG+++G + +L R+ FADRDAGGRGLQTFDLR IH VT +EAA+LDLLGQ Sbjct: 67 DAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQTFDLRTTIHVVTGIEAAMLDLLGQ 126 Query: 141 FLEVPVAALLGEGQQRSEVEMLGYLFYVGDRNKTDLPYLSAPDAADDWLRLRHEAAMTPE 200 L V VA+LLG+GQQRSEVEMLGYLF+VG+R T LPY S PD + DW RLRHE AMTP+ Sbjct: 127 HLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKATPLPYQSQPDDSCDWYRLRHEEAMTPD 186 Query: 201 AVVRLAEAAYARYGFNDFKLKGGVMRGEEEIVAVTALAERFPKARITLDPNGGWLLKDAI 260 AVVRLAEAAY +YGFNDFKLKGGV+ GEEE ++ ALA+RFP+ARITLDPNG W L +AI Sbjct: 187 AVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAESIVALAQRFPQARITLDPNGAWSLNEAI 246 Query: 261 RLCRDQHDVLAYAEDPCGAEEGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQS 320 ++ + LAYAEBPCGAE+G+SGREVMAEFRRATGL TATNM+ATDWRQMGH +SLQS Sbjct: 247 KIGKYLKGSLAYAEBPCGAEQGFSGREVMAEFRRATGLPTATNMIATDWRQMGHTLSLQS 306 Query: 321 VDIPLADPHFWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID 380 VDIPLADPHFWTMQGSVRVAQMC+E+GLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID Sbjct: 307 VDIPLADPHFWTMQGSVRVAQMCHEFGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID 366 Query: 381 THWIWQEG-QRLTREPLQIVGGKVQVPSKPGLGVEVDMAQIEAAHQTYRNMGLGARDDAV 439 THWIWQEG QRLT+EP +I GG VQVP KPGLGVE+DM Q+ AH+ Y+ GLGARDDA+ Sbjct: 367 THWIWQEGNQRLTKEPFEIKGGLVQVPEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAM 426 Query: 440 AMQYLIPGWKFDHKKPCLVR 459 MQYLIPGW FD+K+PC+VR Sbjct: 427 GMQYLIPGWTFDNKRPCMVR 446 >STM2960 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Sty Salmonella typhimurium LT2 Length = 446 Score = 705 bits (1820), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/440 (75%), Positives = 379/440 (86%), Gaps = 1/440 (0%) Query: 21 APRVTEMRVIPVAGHDSMLLNLSGAHGPYFTRNIVILTDSAGHTGVGEVPGGEGIRQTLE 80 P VTEM+VIPVAGHDSML+NLSGAH P+FTRNIVI+ D++GHTGVGE+PGGE IR+TLE Sbjct: 7 TPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIKDNSGHTGVGEIPGGEKIRKTLE 66 Query: 81 DARALVVGQSIGNVQGILNRARSAFADRDAGGRGLQTFDLRIAIHAVTALEAALLDLLGQ 140 DA LVVG+++G + +L R+ FADRDAGGRGLQTFDLR IH VT +EAA+LDLLGQ Sbjct: 67 DAIPLVVGKTLGEYKNVLTAVRNQFADRDAGGRGLQTFDLRTTIHVVTGIEAAMLDLLGQ 126 Query: 141 FLEVPVAALLGEGQQRSEVEMLGYLFYVGDRNKTDLPYLSAPDAADDWLRLRHEAAMTPE 200 L V VA+LLG+GQQRSEVEMLGYLF+VG+R T LPY S PD DW RLRHE AMTPE Sbjct: 127 HLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKATPLPYQSQPDEQCDWYRLRHEEAMTPE 186 Query: 201 AVVRLAEAAYARYGFNDFKLKGGVMRGEEEIVAVTALAERFPKARITLDPNGGWLLKDAI 260 VVRLAEAAY +YGFNDFKLKGGV+ GEEE ++ ALA+RFP+AR+TLDPNG W L +AI Sbjct: 187 TVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAESIVALAKRFPQARVTLDPNGAWSLNEAI 246 Query: 261 RLCRDQHDVLAYAEDPCGAEEGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQS 320 + + LAYAEDPCGAE+G+SGREVMAEFRRATGL TATNM+ATDWRQMGH +SLQS Sbjct: 247 SIGKYLKGSLAYAEDPCGAEQGFSGREVMAEFRRATGLPTATNMIATDWRQMGHTLSLQS 306 Query: 321 VDIPLADPHFWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID 380 VDIPLADPHFWTMQGSVRVAQMC+E+GLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID Sbjct: 307 VDIPLADPHFWTMQGSVRVAQMCHEFGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAID 366 Query: 381 THWIWQEG-QRLTREPLQIVGGKVQVPSKPGLGVEVDMAQIEAAHQTYRNMGLGARDDAV 439 THWIWQEG QRLT+EP +I GG VQVP+KPGLGVE+DM Q+ AH+ Y+ GLGARDDA+ Sbjct: 367 THWIWQEGNQRLTKEPFEIKGGMVQVPTKPGLGVELDMDQVMKAHELYQKHGLGARDDAM 426 Query: 440 AMQYLIPGWKFDHKKPCLVR 459 MQYLIPGW FD+K+PC+VR Sbjct: 427 GMQYLIPGWTFDNKRPCMVR 446 >BS_ycbF [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Bsu Bacillus subtilis Length = 455 Score = 703 bits (1815), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/438 (74%), Positives = 376/438 (85%) Query: 22 PRVTEMRVIPVAGHDSMLLNLSGAHGPYFTRNIVILTDSAGHTGVGEVPGGEGIRQTLED 81 P ++EM+VIPVAGHDSMLLNLSGAH P+FTRNIVILTDS+G+ GVGEVPGGE IR+TLE Sbjct: 18 PSISEMKVIPVAGHDSMLLNLSGAHSPFFTRNIVILTDSSGNQGVGEVPGGEHIRRTLEL 77 Query: 82 ARALVVGQSIGNVQGILNRARSAFADRDAGGRGLQTFDLRIAIHAVTALEAALLDLLGQF 141 + LVVG+SIG Q IL R F D+D GGRG QTFDLR +HAVTALEAALLD LG+F Sbjct: 78 SEPLVVGKSIGAYQAILQTVRKQFGDQDRGGRGNQTFDLRTTVHAVTALEAALLDFLGKF 137 Query: 142 LEVPVAALLGEGQQRSEVEMLGYLFYVGDRNKTDLPYLSAPDAADDWLRLRHEAAMTPEA 201 L+ PVAALLGEG+QR EV+MLGYLFY+GDRN+T LPY S + W RLRHE A+TPEA Sbjct: 138 LQEPVAALLGEGKQRDEVKMLGYLFYIGDRNRTTLPYQSDEQSDCAWFRLRHEEALTPEA 197 Query: 202 VVRLAEAAYARYGFNDFKLKGGVMRGEEEIVAVTALAERFPKARITLDPNGGWLLKDAIR 261 +VRLAE+A RYGF DFKLKGGV+RGEEEI AVTAL++RFP+ARITLDPNG W L++AI Sbjct: 198 IVRLAESAQERYGFQDFKLKGGVLRGEEEIEAVTALSKRFPEARITLDPNGAWSLEEAIA 257 Query: 262 LCRDQHDVLAYAEDPCGAEEGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQSV 321 LC+ + DVLAYAEDPCG E GYS REVMAEFRRATGL TATNM+ATDWR+MGHAI L +V Sbjct: 258 LCKGKQDVLAYAEDPCGDENGYSAREVMAEFRRATGLPTATNMIATDWREMGHAIQLHAV 317 Query: 322 DIPLADPHFWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAIDT 381 DIPLADPHFWTMQGSVRVAQMC++WGLTWGSHSNNHFDISLAMFTHVAAAAPG+ITAIDT Sbjct: 318 DIPLADPHFWTMQGSVRVAQMCHDWGLTWGSHSNNHFDISLAMFTHVAAAAPGRITAIDT 377 Query: 382 HWIWQEGQRLTREPLQIVGGKVQVPSKPGLGVEVDMAQIEAAHQTYRNMGLGARDDAVAM 441 HWIWQ+GQRLT++P +I G V+VP KPGLGV++DM Q+E AH+ YR M LGAR+DA+ M Sbjct: 378 HWIWQDGQRLTKQPFEISSGCVKVPDKPGLGVDIDMEQVEKAHEIYRKMNLGARNDAIPM 437 Query: 442 QYLIPGWKFDHKKPCLVR 459 Q+LI W+FD K+PCLVR Sbjct: 438 QFLISNWEFDRKRPCLVR 455