BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0941.p (402 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2871 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 376 e-104 SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carbox... 359 6e-99 AGl2806 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 353 5e-97 AGc4953 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 352 6e-97 PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 345 1e-94 >RSc2871 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Rso Ralstonia solanacearum Length = 396 Score = 376 bits (966), Expect = e-104, Method: Compositional matrix adjust. Identities = 184/385 (47%), Positives = 244/385 (63%), Gaps = 8/385 (2%) Query: 19 RAHWASLRRDLHAHPELRFEEHRTAEVVVKELQALGYAVTRGLGGTGVVASLPGADPQRG 78 +A +LRRD+HAHPEL FEE RT+++V +L G V RGLG TG+V + + QR Sbjct: 11 QAEIQALRRDIHAHPELCFEEQRTSDLVAAKLAEWGIEVHRGLGKTGLVGVIRNGEGQR- 69 Query: 79 IVLRADLDALPIHEANEFAHASCSHGIMHACGHDGHTVMLLGAARVLKQMPQLPGSVHFV 138 I LRAD+DALP+ EAN+F H S G MHACGHDGHT MLLGAA L + G+VH + Sbjct: 70 IGLRADMDALPLAEANQFTHRSRHEGKMHACGHDGHTAMLLGAAHYLARHRNFSGTVHLI 129 Query: 139 FQPGEEGGAGARKMIDDGLFTQFPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGSRLKITI 198 FQP EEGG GAR+MI DGLF +FP +AVFGMHNWPG+P G FG R GP+MA+ + +I I Sbjct: 130 FQPAEEGGGGAREMIRDGLFDRFPCDAVFGMHNWPGVPVGAFGTRVGPLMASSNEFRIVI 189 Query: 199 TGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDR 258 GKGAHAA PH G DP+ + MV Q + R+K P+D AV+S+ FHAGD N+IP+ Sbjct: 190 KGKGAHAALPHNGNDPVFVGAQMVSALQGVITRNKRPIDTAVLSITQFHAGDASNIIPNE 249 Query: 259 AELRGTIRTLSSTLQQKLQEDIRRMCQALAEAYGAQVEVEFFQYYPATINTPAETDFCER 318 A + GT+RT S+ + ++ + + +A+A AY ++ F + YP T+NT ET F Sbjct: 250 AWIGGTVRTFSTAVLDLIERRMEEVAKAIAAAYDCSIDFTFHRNYPPTVNTERETLFAAE 309 Query: 319 VIRQTFGDARIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPADRPSHS-------LHHP 371 V+R+ G + I M +EDF FML E+PG + IGN D LH+P Sbjct: 310 VMRELVGPDHVDANIDPTMGAEDFSFMLIEKPGCFAFIGNGDGDHREQGHGLGPCMLHNP 369 Query: 372 HYDFNDDIIEEGVRYWVALAQSYFS 396 YDFND+++ G YWV L + + + Sbjct: 370 SYDFNDELLPLGATYWVRLVEKFLA 394 >SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Sme Sinorhizobium meliloti Length = 389 Score = 359 bits (921), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 179/386 (46%), Positives = 244/386 (63%), Gaps = 3/386 (0%) Query: 12 AASVGSHRAHWASLRRDLHAHPELRFEEHRTAEVVVKELQALGYAVTRGLGGTGVVASLP 71 +A++ + ++RRDLHAHPEL EE RT+ + + L+ LGY V G+ TGVV +L Sbjct: 4 SAAIDNAMPELVAIRRDLHAHPELGLEETRTSAFIARHLEELGYEVATGIAKTGVVGTLR 63 Query: 72 GADPQRGIVLRADLDALPIHEANEFAHASCSHGIMHACGHDGHTVMLLGAARVLKQMPQL 131 R I +RAD+DALPI E A+AS G+MHACGHDGHT MLLGAAR L + Sbjct: 64 NGTGSRSIGIRADIDALPIQEETGVAYASTKPGLMHACGHDGHTAMLLGAARALAERRNF 123 Query: 132 PGSVHFVFQPGEEGGAGARKMIDDGLFTQFPTEAVFGMHNWPGLPAGQFGLRTGPIMAAG 191 G++H +FQP EE GA+ M+D+GLF +FP +AVF +HN P LP GQF LR GPIMAA Sbjct: 124 DGTIHLIFQPAEENAGGAKIMVDEGLFDRFPCDAVFALHNEPNLPFGQFALREGPIMAAV 183 Query: 192 SRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDT 251 +IT+ G+G H A+P DPI S+V+ QTI AR+ P+DP+V++V FHAG Sbjct: 184 DEARITVHGRGGHGAEPQATADPIVCGASIVMALQTIVARNIHPMDPSVVTVGAFHAGSA 243 Query: 252 DNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCQALAEAYGAQVEVEFFQYYPATINTPA 311 N+IP+RAE+ IR+ ++ +L+ IR + +A A ++G + V++ + Y ATIN A Sbjct: 244 SNIIPERAEIVVGIRSFDPAVRDELERRIRMIAEAQASSFGMRATVDYERSYDATINHKA 303 Query: 312 ETDFC-ERVIRQTFGDARIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPADRPSHSLHH 370 ETDF E IR D + P M SEDF +ML+ERPG+Y +G+ SLHH Sbjct: 304 ETDFLREAAIRFAGADKVVDLARPF-MGSEDFAYMLKERPGSYFFLGSRVTGE-EKSLHH 361 Query: 371 PHYDFNDDIIEEGVRYWVALAQSYFS 396 P YDFNDD++ G +W LA++Y + Sbjct: 362 PGYDFNDDLLPIGAAFWTELAEAYLA 387 >AGl2806 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 422 Score = 353 bits (905), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 173/373 (46%), Positives = 236/373 (63%), Gaps = 4/373 (1%) Query: 24 SLRRDLHAHPELRFEEHRTAEVVVKELQALGYAVTRGLGGTGVVASLPGADPQRGIVLRA 83 ++R DLH HPEL +E RTA ++ + L + GY VT G+GG GVVA+L ++ + +RA Sbjct: 54 AIRHDLHRHPELSLKETRTAALIARYLLSFGYEVTEGVGGHGVVATLKRGHGEKRLGIRA 113 Query: 84 DLDALPIHEANEFAHASCSHGIMHACGHDGHTVMLLGAARVLKQMPQLPGSVHFVFQPGE 143 D+DALPIHE A+AS + GIMHACGHDGHT +LL AAR L + G++ +FQP E Sbjct: 114 DIDALPIHEETGLAYASETSGIMHACGHDGHTTVLLAAARYLAETSNFSGTLTLIFQPAE 173 Query: 144 EGGAGARKMIDDGLFTQFPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGSRLKITITGKGA 203 E GAGAR MI D LF +FP +AVFG+HNWPG+ GQFG GP MA+ + + I GKG Sbjct: 174 EIGAGARAMIKDRLFERFPVDAVFGLHNWPGVETGQFGFVEGPAMASVDKASVRIIGKGG 233 Query: 204 HAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDRAELRG 263 H A PH +DP+ + S + Q+I +R+ DP + AV++V H G NVIP+ EL+ Sbjct: 234 HGAAPHETVDPVLASASFITALQSIVSRNIDPREMAVLTVGSIHGGTASNVIPESVELQI 293 Query: 264 TIRTLSSTLQQKLQEDIRRMCQALAEAYGAQVEVEFFQYYPATINTPAETDFCERVIRQT 323 T R S+ ++ +L+E + + +A AE++GA +V + + +P IN ET F V R+ Sbjct: 294 TARAFSTAVRDRLEERLPALAKAQAESFGATADVSYSRGFPPVINHAPETAFARDVARRH 353 Query: 324 FGDARIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPADRPSHSLHHPHYDFNDDIIEEG 383 F A+I SEDF FML+ERPG+Y+ +GN S LH PHYDFND II Sbjct: 354 FPAAQIADAFQPRTASEDFAFMLQERPGSYLFVGNG----ESAPLHSPHYDFNDAIIPPA 409 Query: 384 VRYWVALAQSYFS 396 R+WV LA++Y S Sbjct: 410 ARFWVRLAEAYLS 422 >AGc4953 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 393 Score = 352 bits (904), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 172/387 (44%), Positives = 245/387 (63%), Gaps = 1/387 (0%) Query: 11 DAASVGSHRAHWASLRRDLHAHPELRFEEHRTAEVVVKELQALGYAVTRGLGGTGVVASL 70 + AS +H ++RR LH HPE+ E +T++ + ++L +GY VTRGL GTG+VA+L Sbjct: 7 NIASFANHMPDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATL 66 Query: 71 PGADPQRGIVLRADLDALPIHEANEFAHASCSHGIMHACGHDGHTVMLLGAARVLKQMPQ 130 D R + +RAD+DALPIHE +AS G+MHACGHDGHT MLLGAA+++ + Sbjct: 67 RNGDSTRALGIRADIDALPIHEETGADYASTHQGVMHACGHDGHTAMLLGAAKIIAERRN 126 Query: 131 LPGSVHFVFQPGEEGGAGARKMIDDGLFTQFPTEAVFGMHNWPGLPAGQFGLRTGPIMAA 190 G++H +FQP EE GAR MI+DGLF +FP +AVF +HN PGLP GQF LR GPI+AA Sbjct: 127 FDGTLHLIFQPAEENFGGARIMIEDGLFERFPCDAVFALHNDPGLPFGQFVLRDGPILAA 186 Query: 191 GSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGD 250 KIT+ G G H A+P DPI S+++ QT+ +R+ P AV++V FHAG Sbjct: 187 VDECKITVRGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGM 246 Query: 251 TDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCQALAEAYGAQVEVEFFQYYPATINTP 310 NVIP+ AE+ TIR+ ++ +L++ IR + + A +YG V +++ + Y AT+N Sbjct: 247 ASNVIPETAEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHK 306 Query: 311 AETDFCERVIRQTFGDARIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPADRPSHSLHH 370 AETD+ + R+ G ++ +M +EDF +MLE+RPG Y +G A D LHH Sbjct: 307 AETDYVADLARRFAGPEKVLEMKRPSMGAEDFAYMLEKRPGCYFFLGTARTDNDP-PLHH 365 Query: 371 PHYDFNDDIIEEGVRYWVALAQSYFSS 397 P +DFND+I+ G +WV LA+ Y + Sbjct: 366 PKFDFNDEILPIGTTFWVDLAEDYLKA 392 >PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Pae Pseudomonas aeruginosa Length = 406 Score = 345 bits (885), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 172/378 (45%), Positives = 238/378 (62%), Gaps = 3/378 (0%) Query: 24 SLRRDLHAHPELRFEEHRTAEVVVKELQALGYAVTRGLGGTGVVASLPGADPQRGIVLRA 83 SLR+D+HAHPEL FEE RTA +V + L+ GY V G+G TGVV L D R + LRA Sbjct: 20 SLRQDIHAHPELGFEERRTAALVAECLRGWGYEVHEGIGRTGVVGVLRQGDGTRRLGLRA 79 Query: 84 DLDALPIHEANEFAHASCSHGIMHACGHDGHTVMLLGAARVLKQMPQLPGSVHFVFQPGE 143 D+DALPI EA ++SC G MHACGHDGHT MLLGAAR L + G++ +FQP E Sbjct: 80 DMDALPIVEATGLGYSSCHGGRMHACGHDGHTAMLLGAARYLAATRRFDGTLVLIFQPAE 139 Query: 144 EGGAGARKMIDDGLFTQFPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGSRLKITITGKGA 203 EG GA M+ DGL +FP +A+FGMHN PGL AG G R GP+MA+ L +T+ G G Sbjct: 140 EGQGGAEAMLADGLLERFPCDALFGMHNMPGLEAGHLGFRAGPMMASQDLLSVTLEGVGG 199 Query: 204 HAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDRAELRG 263 H + PHL +DP+ A S V+ Q++ AR+ DP AV++V AG+ NVIP RA LR Sbjct: 200 HGSMPHLSVDPLLAASSAVMALQSVVARNVDPQKAAVVTVGALQAGEAANVIPQRAVLRL 259 Query: 264 TIRTLSSTLQQKLQEDIRRMCQALAEAYGAQVEVEFFQYYPATINTPAETDFCERVIRQT 323 ++R L +++++ + +R++ + A +YG Q +E + YP +N+ ET+F +V + Sbjct: 260 SLRALDGQVREQVLQRVRQIIELQAASYGCQASIEHYPAYPVLVNSVEETEFARQVGVEL 319 Query: 324 FGDARIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPADRPSHSLHHPHYDFNDDIIEEG 383 G ++ P M SEDF +ML+ PG+Y+ IGN R +H+P YDFNDDI+ G Sbjct: 320 AGAEQVDGDTPKLMGSEDFAWMLQRCPGSYLFIGNG---RGRPMVHNPAYDFNDDILVRG 376 Query: 384 VRYWVALAQSYFSSDLTS 401 YW ALA+++ ++ S Sbjct: 377 AAYWGALAETWLAAPAAS 394