BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0871.p (443 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3595 [GEPR] COG0477 Permeases of the major facilitator superfa... 312 9e-85 ECs2778 [GEPR] COG0477 Permeases of the major facilitator superf... 264 3e-70 ZshiA [GEPR] COG0477 Permeases of the major facilitator superfam... 264 4e-70 shiA [GEPR] COG0477 Permeases of the major facilitator superfami... 263 4e-70 RSp0985 [GEPR] COG0477 Permeases of the major facilitator superf... 260 4e-69 >PA3595 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 469 Score = 312 bits (800), Expect = 9e-85, Method: Compositional matrix adjust. Identities = 192/430 (44%), Positives = 262/430 (60%), Gaps = 14/430 (3%) Query: 2 PTTPPRHQPVR-ASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTT-NGFYGTLASFGT 59 P H+ R A +A+F+GT IEWYDFYIY A+ALVFGK+FFP + TL SF T Sbjct: 7 PEDADLHKKARRAGIASFIGTTIEWYDFYIYGLAAALVFGKVFFPADMDPGLATLLSFVT 66 Query: 60 FAVGFLARPLGGAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPIALV 119 GF+ARPLGG +FG +GDR+GRK +LVITL+MMG T IGLLP Y IGIWAPI+LV Sbjct: 67 LWSGFIARPLGGIIFGHLGDRIGRKTTLVITLIMMGIATTCIGLLPVYTQIGIWAPISLV 126 Query: 120 ALRFIQGIAVGGEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMFKLVSGMD 179 ALR +QGIAVGGEWGGA+L+ASE +P + + A+FAQ GSP G +LATL F +S + Sbjct: 127 ALRIVQGIAVGGEWGGAILIASESAP-KGKGILYAAFAQQGSPTGNLLATLAFFALSALP 185 Query: 180 NAVFLDWGWRLPFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAPLAEV 239 + F+ WGWR PFL+SA+L++VG IRL ++ES D ++ + L PLAEV Sbjct: 186 ASDFILWGWRAPFLLSAVLVIVGMVIRLKLEESEDMKRVLAQKRTVKL-------PLAEV 238 Query: 240 LRGAPGLLVLAIGANVLGIAGF-YFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQFFIT 298 L G +VL +GA VL I YF +TF L + T+ L +G L+ ++V V+QF Sbjct: 239 L-GKHWKIVL-LGAGVLPIIHVTYFKSTFALSWATKTLGYSQGTFLSIIVVALVVQFLSQ 296 Query: 299 PLAAHLARKVGNHRFLVMMSALSIVSPYAMFSLVDLGSLASVTLGVAIAVLFIGAYYAVI 358 PL A L ++ + +M + AMF V+ +G+ +A + +Y + Sbjct: 297 PLGALLTSRIDMRKAACLMVLPEFILMPAMFFAVETKVYWIAAVGMCLATVPHAMFYGAV 356 Query: 359 AGFVSDAFPTRIRYTGISMAYQVSGALFGGLTPLLGTVLAEKFQGHWWPLALLFSGISLL 418 G ++ AFP R+RYTG+S+AYQ+ L GG TP+L + G +AL + +L+ Sbjct: 357 GGILARAFPARVRYTGLSLAYQLCSLLVGGGTPVLAQWILNS-TGSIVGVALASACYALV 415 Query: 419 SMLCVARLSR 428 S+ C L R Sbjct: 416 SLACTLALLR 425 >ECs2778 [GEPR] COG0477 Permeases of the major facilitator superfamily Ecs Escherichia coli O157:H7 Length = 438 Score = 264 bits (675), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 160/437 (36%), Positives = 243/437 (55%), Gaps = 15/437 (3%) Query: 3 TTPPRHQPV------RASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLAS 56 +T P + V RA+L +F G +++WYDF +Y +ALVF + FFP + GTLA+ Sbjct: 7 STRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAA 66 Query: 57 FGTFAVGFLARPLGGAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPI 116 F TF VGFL RPLGG +FG GDRLGRK+ L++T+ MMG T +IG+LP++++IG WAPI Sbjct: 67 FATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPI 126 Query: 117 ALVALRFIQGIAVGGEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMFKLVS 176 LV LR IQG AVGGEWGGA L++ E +P ++++ F +S Q+G GL+L+T + L+S Sbjct: 127 LLVTLRAIQGFAVGGEWGGAALLSVESAP-KNKKAFYSSGVQVGYGVGLLLSTGLVSLIS 185 Query: 177 GM-DNAVFLDWGWRLPFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAP 235 M + FL WGWR+PFL S +L++ +R ++ES +F +Q +A+A P Sbjct: 186 MMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQAAA-----KKRIP 240 Query: 236 LAEVLRGAPGLLVLAIGANVLGIAGFYFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQF 295 + E L PG + I + + Y F L Y+TQ + L R L LN L+V + Sbjct: 241 VIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSC 300 Query: 296 FITPLAAHLARKVGNHRFLVMMSALSIVSPYAMFSLVDLGSLASVT-LGVAIAVLFIGAY 354 P A LA + G R + + + +S + F ++ S+ + + +A + Sbjct: 301 LTIPCFAWLADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMV 360 Query: 355 YAVIAGFVSDAFPTRIRYTGISMAYQVSGALFGGLTPLLGTVLAEKFQGHWWPLAL-LFS 413 V ++ F RY+G + YQV+ + GG TP + L F G+W +A+ L + Sbjct: 361 VCVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLA 420 Query: 414 GISLLSMLCVARLSRQR 430 G + +M + QR Sbjct: 421 GCLISAMTALLMKDNQR 437 >ZshiA [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 438 Score = 264 bits (674), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 159/437 (36%), Positives = 242/437 (55%), Gaps = 15/437 (3%) Query: 3 TTPPRHQPV------RASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLAS 56 +T P + V RA+L +F G +++WYDF +Y +ALVF + FFP + GTLA+ Sbjct: 7 STRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAA 66 Query: 57 FGTFAVGFLARPLGGAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPI 116 F TF VGFL RPLGG +FG GDRLGRK+ L++T+ MMG T +IG+LP++++IG WAPI Sbjct: 67 FATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPI 126 Query: 117 ALVALRFIQGIAVGGEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMFKLVS 176 LV LR IQG AVGGEWGGA L++ E +P ++++ F +S Q+G GL+L+T + L+S Sbjct: 127 LLVTLRAIQGFAVGGEWGGAALLSVESAP-KNKKAFYSSGVQVGYGVGLLLSTGLVSLIS 185 Query: 177 GM-DNAVFLDWGWRLPFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAP 235 M + FL WGWR+PFL S +L++ +R ++ES +F +Q +A+A P Sbjct: 186 MMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQAAA-----KKRIP 240 Query: 236 LAEVLRGAPGLLVLAIGANVLGIAGFYFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQF 295 + E L PG + I + + Y F L Y+TQ + L R L LN L+V + Sbjct: 241 VIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSC 300 Query: 296 FITPLAAHLARKVGNHRFLVMMSALSIVSPYAMFSLVDLGSLASVT-LGVAIAVLFIGAY 354 P A A + G R + + + +S + F ++ S+ + + +A + Sbjct: 301 LTIPCFAWXADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMV 360 Query: 355 YAVIAGFVSDAFPTRIRYTGISMAYQVSGALFGGLTPLLGTVLAEKFQGHWWPLAL-LFS 413 V ++ F RY+G + YQV+ + GG TP + L F G+W +A+ L + Sbjct: 361 VCVQQPMFTEMFGASYRYSGAGVGYQVASVVXGGFTPFIAAALITYFAGNWHSVAIYLLA 420 Query: 414 GISLLSMLCVARLSRQR 430 G + +M + QR Sbjct: 421 GCLISAMTALLMKDNQR 437 >shiA [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 438 Score = 263 bits (673), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 157/422 (37%), Positives = 238/422 (56%), Gaps = 9/422 (2%) Query: 12 RASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLASFGTFAVGFLARPLGG 71 RA+L +F G +++WYDF +Y +ALVF + FFP + GTLA+F TF VGFL RPLGG Sbjct: 22 RAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGG 81 Query: 72 AVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPIALVALRFIQGIAVGG 131 +FG GDRLGRK+ L++T+ MMG T +IG+LP++++IG WAPI LV LR IQG AVGG Sbjct: 82 VIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGG 141 Query: 132 EWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMFKLVSGM-DNAVFLDWGWRL 190 EWGGA L++ E +P ++++ F +S Q+G GL+L+T + L+S M + FL WGWR+ Sbjct: 142 EWGGAALLSVESAP-KNKKAFYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRI 200 Query: 191 PFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAPLAEVLRGAPGLLVLA 250 PFL S +L++ +R ++ES +F +Q +A+A P+ E L PG + Sbjct: 201 PFLFSIVLVLGALWVRNGMEESAEFEQQQHYQAAA-----KKRIPVIEALLRHPGAFLKI 255 Query: 251 IGANVLGIAGFYFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQFFITPLAAHLARKVGN 310 I + + Y F L Y+TQ + L R L LN L+V + P A LA + G Sbjct: 256 IALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGR 315 Query: 311 HRFLVMMSALSIVSPYAMFSLVDLGSLASVT-LGVAIAVLFIGAYYAVIAGFVSDAFPTR 369 R + + + +S + F ++ S+ + + +A + V ++ F Sbjct: 316 RRVYITGTLIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGAS 375 Query: 370 IRYTGISMAYQVSGALFGGLTPLLGTVLAEKFQGHWWPLAL-LFSGISLLSMLCVARLSR 428 RY+G + YQV+ + GG TP + L F G+W +A+ L +G + +M + Sbjct: 376 YRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDS 435 Query: 429 QR 430 QR Sbjct: 436 QR 437 >RSp0985 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 409 Score = 260 bits (665), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 159/389 (40%), Positives = 227/389 (58%), Gaps = 14/389 (3%) Query: 11 VRASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLASFGTFAVGFLARPLG 70 +RASLA+ +G+ IEWYDF++Y ASALVF ++FFP G +A+FGT+ +GF+ RPLG Sbjct: 1 MRASLASLLGSTIEWYDFFLYGAASALVFNQVFFPKLGALSGAIAAFGTYGLGFVVRPLG 60 Query: 71 GAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPIALVALRFIQGIAVG 130 FG GDRLGRK L+ +LL MG TV+IGLLP+Y +IG AP+ L+ LR +QG AVG Sbjct: 61 ALAFGHFGDRLGRKTVLLASLLAMGLPTVLIGLLPSYDAIGYLAPLLLIVLRLVQGFAVG 120 Query: 131 GEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMFKLVSGMDNAVFLDWGWRL 190 GEW GAV++A EH+ R + L +Q G AGL L++L VS + + L W WR+ Sbjct: 121 GEW-GAVILAVEHASPRF-KGLLGGLSQTGVAAGLTLSSLAMAAVSAIGHEAMLSWAWRI 178 Query: 191 PFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAPLAEVLRGAPGLLVLA 250 PF+ S LL+ +G+ +R V E+P+F ASA + P++ V + G L+ Sbjct: 179 PFVASGLLVALGWLLRRKVDETPEF-------ASAKQQGRCLKYPVSNVFKDHAGALLSV 231 Query: 251 IGANVLGIAGFYFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQFFITPLAAHLARKVGN 310 GA V I+ FY F L Y T+ L L LNG+ + + F+ PL L + G Sbjct: 232 AGARVAEISFFYIVTAFTLWYATKQLGLPGAWCLNGVTLGAAAATFLMPLCGMLGDRWGA 291 Query: 311 HRFLV--MMSALSIVSPYAMFSLVDLGSLASVTLGVAIAVLFIGAYYAVIAGFVSDAFPT 368 R + +++AL + P+ F LV+ ++ V L A++V+ + A A FP Sbjct: 292 RRIYIAGIVTALLWILPF--FMLVNTRAMVWVVLAEAVSVVLSFSMAAQQASLFVQQFPV 349 Query: 369 RIRYTGISMAYQVSGALFGGLTPLLGTVL 397 RYTG S+A ++GAL GGL P++ T L Sbjct: 350 IARYTGASLAVNIAGAL-GGLAPIVATAL 377