BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0859.p (243 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl1076 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 427 e-119 BMEII0980 Non COG 270 3e-72 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 109 7e-24 SA2266 [R] COG4221 Short-chain alcohol dehydrogenase of unknown ... 106 6e-23 YMR226c [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 103 3e-22 >AGl1076 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 243 Score = 427 bits (1097), Expect = e-119, Method: Compositional matrix adjust. Identities = 205/243 (84%), Positives = 219/243 (90%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEELAARLGPKAIPQVT 60 M++SL GKIAVITGAASGIGLATTE LL ATVVMVDWN KAL +LAA+LG +AIPQVT Sbjct: 1 MSESLQGKIAVITGAASGIGLATTEALLEQGATVVMVDWNEKALNDLAAKLGERAIPQVT 60 Query: 61 NLLDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSAEAIDKMLNLNVNAVMKNV 120 NLLDADSC AM+PE+L KV HIDILYCNAGTYIGG+L +T+ EAIDKMLNLNVNAVMKNV Sbjct: 61 NLLDADSCNAMIPEILGKVDHIDILYCNAGTYIGGDLTETTPEAIDKMLNLNVNAVMKNV 120 Query: 121 HAVAPHMIARKTGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 AV PHM RKTGDIIVTCS+AGHFPTYWEPVY+GSKWAIT FVQ MRRQMIPHGVRV Q Sbjct: 121 QAVVPHMSERKTGDIIVTCSIAGHFPTYWEPVYSGSKWAITSFVQGMRRQMIPHGVRVAQ 180 Query: 181 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLPTNF 240 VSPGPV+SALLADWPEENLRKAKESGSLI+ SEVADAV YMLTR R VTIRDMLVLPTNF Sbjct: 181 VSPGPVVSALLADWPEENLRKAKESGSLIDASEVADAVVYMLTRKRTVTIRDMLVLPTNF 240 Query: 241 DRV 243 DRV Sbjct: 241 DRV 243 >BMEII0980 Non COG Length = 242 Score = 270 bits (689), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 126/241 (52%), Positives = 171/241 (70%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEELAARLGPKAIPQVT 60 M Q L G++A +TGAASGIG + ++ TV +VD + AL E LG KA P V Sbjct: 1 MAQDLNGRVAAVTGAASGIGFECAKHMIGAGITVFLVDRDENALAEKCVELGDKAKPLVV 60 Query: 61 NLLDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSAEAIDKMLNLNVNAVMKNV 120 +LLD S A M+P++L++ +DI + NAG+YIGG L + +A D+MLNLN+NA + V Sbjct: 61 DLLDPASVATMMPQILEQAGQLDIFHANAGSYIGGPLWEGDPDAWDRMLNLNINAAFRTV 120 Query: 121 HAVAPHMIARKTGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 HAV PHM+ RKTGDII+T SVAG P WEP+Y SK A+ FV T+RRQ+ PHG+RVG Sbjct: 121 HAVLPHMMERKTGDIIMTSSVAGVVPVIWEPIYTASKHAVQAFVHTVRRQVAPHGIRVGG 180 Query: 181 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLPTNF 240 V PGPV++AL++DWP+ L +A +GSL+EP EVA++V +MLTR RN+TIRD+++LP + Sbjct: 181 VLPGPVVTALISDWPQAKLDEALAAGSLMEPKEVAESVMFMLTRPRNITIRDLVILPQSL 240 Query: 241 D 241 D Sbjct: 241 D 241 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 246 Score = 109 bits (272), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 75/227 (33%), Positives = 112/227 (49%), Gaps = 14/227 (6%) Query: 5 LAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEEL---AARLGPKAIPQVTN 61 L GK+ +ITGAASGIG ATT ATV+ D + + L+ L A L K P V N Sbjct: 3 LEGKVCLITGAASGIGKATTLLFAQEGATVIAGDISKENLDSLVKEAEGLPGKVDPYVLN 62 Query: 62 LLDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSAEAIDKMLNLNVNAVMKNVH 121 + D D +V +V+QK ID+L NAG LV E D ++N+N+ V Sbjct: 63 VTDRDQIKEVVEKVVQKYGRIDVLVNNAGITRDALLVRMKEEDWDAVINVNLKGVFNVTQ 122 Query: 122 AVAPHMIARKTGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQV 181 V P+MI ++ G I+ SV G + + YA SK + +T +++ +RV V Sbjct: 123 MVVPYMIKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVNAV 182 Query: 182 SPGPVISALLADWPEENLRKAKES-------GSLIEPSEVADAVEYM 221 +PG + + + PE KA+E+ G +P EVA + ++ Sbjct: 183 APGFIETPMTEKLPE----KARETALSRIPLGRFGKPEEVAQVILFL 225 >SA2266 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Sau Staphylococcus aureus N315 Length = 231 Score = 106 bits (264), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 64/234 (27%), Positives = 121/234 (51%), Gaps = 8/234 (3%) Query: 5 LAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEELAARLGPKAIPQV-TNLL 63 L KIAV+TGA SGIG A L A VV+ N L+ +A +L ++ V T++ Sbjct: 4 LTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQNVANQLAQDSVKVVPTDVT 63 Query: 64 DADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSAEAIDKMLNLNVNAVMKNVHAV 123 + + ++ Q +DI+ +AG + ++ D + D M+++N+ + A Sbjct: 64 NKEEVDELMKIAQQTFGGLDIVINSAGQMLSSKITDYQVDEWDSMIDVNIKGTLYTAQAA 123 Query: 124 APHMIARKTGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQVSP 183 P M+ + +G +I S++G T +Y+ +K A+ Q + +++ GV+V +SP Sbjct: 124 LPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAVHTITQGLEKELAKTGVKVTSISP 183 Query: 184 GPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLP 237 G V +A+ A + + +K ++P ++A+AV Y LT+ ++V + ++ V P Sbjct: 184 GMVDTAITAAYNPSDRKK-------LDPQDIAEAVLYALTQPKHVNVNEITVRP 230 >YMR226c [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Sce Saccharomyces cerevisiae Length = 267 Score = 103 bits (257), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 77/251 (30%), Positives = 124/251 (49%), Gaps = 16/251 (6%) Query: 3 QSLAGKIAVITGAASGIGLATTEKLLA---NDATVVMVDWNAKALEELAARLG---PKAI 56 + LA K +ITGA++GIG AT + L D +++ + LEEL + P A Sbjct: 9 ERLAKKTVLITGASAGIGKATALEYLEASNGDMKLILAARRLEKLEELKKTIDQEFPNAK 68 Query: 57 PQVTNL--LDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELV-DTSAEAIDKMLNLNV 113 V L A+ + + Q+ IDIL NAG +G + V + E I + + NV Sbjct: 69 VHVAQLDITQAEKIKPFIENLPQEFKDIDILVNNAGKALGSDRVGQIATEDIQDVFDTNV 128 Query: 114 NAVMKNVHAVAPHMIARKTGDIIVTCSVAGH--FPTYWEPVYAGSKWAITCFVQTMRRQM 171 A++ AV P A+ +GDI+ S+AG +PT +Y SK+A+ F ++R+++ Sbjct: 129 TALINITQAVLPIFQAKNSGDIVNLGSIAGRDAYPT--GSIYCASKFAVGAFTDSLRKEL 186 Query: 172 IPHGVRVGQVSPGPV---ISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNV 228 I +RV ++PG V S + EE + + + + +VAD + Y +R +N Sbjct: 187 INTKIRVILIAPGLVETEFSLVRYRGNEEQAKNVYKDTTPLMADDVADLIVYATSRKQNT 246 Query: 229 TIRDMLVLPTN 239 I D L+ PTN Sbjct: 247 VIADTLIFPTN 257