BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0841.p (258 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cj0485 [IQR] COG1028 Dehydrogenases with different specificities... 260 2e-69 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 132 6e-31 BS_yhdF [IQR] COG1028 Dehydrogenases with different specificitie... 124 2e-28 alr5182 [IQR] COG1028 Dehydrogenases with different specificitie... 120 2e-27 SMa0187 [IQR] COG1028 Dehydrogenases with different specificitie... 119 6e-27 >Cj0485 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Cje Campylobacter jejuni Length = 262 Score = 260 bits (664), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 124/259 (47%), Positives = 172/259 (66%), Gaps = 2/259 (0%) Query: 1 VDLNLQEKVVIVTGGASGIGGAISMQLAAEGAIPVVFARSEPEPQFWSRLTALQPRAAWF 60 +DL ++ KV I+TGGA GIG I+ A+EG IPV+F+RS P+ + L L ++ Sbjct: 1 MDLKIKNKVCIITGGAKGIGYGIAKLWASEGGIPVIFSRSMPK-EHDKELKKLSSEYEFY 59 Query: 61 QLELQDAARCGEAVAQTVARFGRLDGLVNNAGVNDSVGL-DAGRDEFVASLERNLIHYYV 119 +++L++ + + V + + G + LVNNAG ND++ + + + + S E NL HYY Sbjct: 60 EIDLKNYEQIEKLVKKVAIKHGGIYALVNNAGTNDNLHIENTSTQDLIKSYENNLFHYYT 119 Query: 120 MAHYCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVN 179 M C+P++K +G+ILN+ SKT +TGQG TS Y ++K AQ+ TREWA A D VRVN Sbjct: 120 MTKECLPYIKKEQGSILNIVSKTGITGQGRTSAYASAKAAQMGFTREWACAFAKDNVRVN 179 Query: 180 ALIPAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHT 239 A+ PAEVMTPLYEKW+ F +P+E+ + I IPLG RFTT EE+A+ AVF LS +SHT Sbjct: 180 AIAPAEVMTPLYEKWLQNFPNPKEQYEKIAKAIPLGHRFTTIEEIANTAVFTLSPLASHT 239 Query: 240 TGQWVFVDGGYTHLDRALT 258 TGQ + DGGY HLDRAL Sbjct: 240 TGQILMPDGGYVHLDRALN 258 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 246 Score = 132 bits (333), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 85/250 (34%), Positives = 133/250 (53%), Gaps = 10/250 (4%) Query: 3 LNLQEKVVIVTGGASGIGGAISMQLAAEGAIPVVFARS-EPEPQFWSRLTALQPRAAWFQ 61 + L+ KV ++TG ASGIG A ++ A EGA + S E L + + Sbjct: 1 MRLEGKVCLITGAASGIGKATTLLFAQEGATVIAGDISKENLDSLVKEAEGLPGKVDPYV 60 Query: 62 LELQDAARCGEAVAQTVARFGRLDGLVNNAGVN-DSVGLDAGRDEFVASLERNLIHYYVM 120 L + D + E V + V ++GR+D LVNNAG+ D++ + +++ A + NL + + Sbjct: 61 LNVTDRDQIKEVVEKVVQKYGRIDVLVNNAGITRDALLVRMKEEDWDAVINVNLKGVFNV 120 Query: 121 AHYCVPHL-KATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVN 179 VP++ K G+I+NVSS + G + Y ASK + +T+ WA L +RVN Sbjct: 121 TQMVVPYMIKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVN 180 Query: 180 ALIPAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHT 239 A+ P + TP+ EK P++ + S+IPLG RF EE+A + +FL S SS+ Sbjct: 181 AVAPGFIETPMTEKL------PEKARETALSRIPLG-RFGKPEEVAQVILFLASDESSYV 233 Query: 240 TGQWVFVDGG 249 TGQ + +DGG Sbjct: 234 TGQVIGIDGG 243 >BS_yhdF [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bsu Bacillus subtilis Length = 289 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 93/251 (37%), Positives = 131/251 (52%), Gaps = 15/251 (5%) Query: 5 LQEKVVIVTGGASGIGGAISMQLAAEGA-IPVVF--ARSEPEPQFWSRLTALQPRAAWFQ 61 L+ KV I+TGG SGIG A ++ A EGA I +++ S+ E + R+ R Sbjct: 43 LKGKVAIITGGDSGIGRAAAIAFAKEGADISILYLDEHSDAE-ETRKRIEKENVRCLLIP 101 Query: 62 LELQDAARCGEAVAQTVARFGRLDGLVNNAG---VNDSVGLDAGRDEFVASLERNLIHYY 118 ++ D C +AV QTV FG+LD LVNNA DS+ L+ ++ + N+ + Sbjct: 102 GDVGDENHCEQAVQQTVDHFGKLDILVNNAAEQHPQDSI-LNISTEQLEKTFRTNIFSMF 160 Query: 119 VMAHYCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRV 178 M +PHL+ AI+N +S TA G Y ++KGA +S TR A +L D G+RV Sbjct: 161 HMTKKALPHLQEG-CAIINTTSITAYEGDTALIDYSSTKGAIVSFTRSMAKSLADKGIRV 219 Query: 179 NALIPAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSH 238 NA+ P + TPL ATF P+EK+ P+G R E A V L S SS+ Sbjct: 220 NAVAPGPIWTPLIP---ATF--PEEKVKQHGLDTPMG-RPGQPVEHAGAYVLLASDESSY 273 Query: 239 TTGQWVFVDGG 249 TGQ + V+GG Sbjct: 274 MTGQTIHVNGG 284 >alr5182 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Nos Nostoc sp. PCC 7120 Length = 285 Score = 120 bits (302), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 84/251 (33%), Positives = 132/251 (52%), Gaps = 13/251 (5%) Query: 4 NLQEKVVIVTGGASGIGGAISMQLAAEGA-IPVVFARSEPEPQFWSRLTALQ-PRAAWFQ 61 L++KV ++TGG SGIG A+++ A EGA + V+ + + L Q RA Sbjct: 38 KLKDKVALITGGDSGIGRAVAIAYAKEGADVAFVYLSEHDDAEETKNLVEEQGRRAVSIA 97 Query: 62 LELQDAARCGEAVAQTVARFGRLDGLVNNAG---VNDSVGLDAGRDEFVASLERNLIHYY 118 ++ D A C A+ QTV FG+LD L+NNA +S+ D +++ + N+ + Sbjct: 98 GDITDEAFCQRAIQQTVDEFGKLDILINNAAEQHPQESIE-DITKEQLERTFRTNIFSMF 156 Query: 119 VMAHYCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRV 178 + + HLK AI+N +S TA G Y ++KGA ++ TR + L G+RV Sbjct: 157 YLTKAAIKHLKKG-SAIINTTSVTAYKGSPQLLDYSSTKGAIVAFTRSLSQNLISKGIRV 215 Query: 179 NALIPAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSH 238 NA+ P + TPL +TF P EK++ ++P+ +R EE+A VFL S SS+ Sbjct: 216 NAVAPGPIWTPLIP---STF--PAEKVETFGKQVPM-QRAGQPEEVAPSYVFLASDDSSY 269 Query: 239 TTGQWVFVDGG 249 +GQ + +GG Sbjct: 270 MSGQVLHPNGG 280 >SMa0187 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 256 Score = 119 bits (298), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 86/252 (34%), Positives = 132/252 (52%), Gaps = 12/252 (4%) Query: 4 NLQEKVVIVTGGASGIGGAISMQLAAEGAIPVVFARSEPEPQFWSRLTALQP--RAAWFQ 61 L++KV ++TGG SGIG ++++ A EGA + E + ++ + + + Sbjct: 12 KLKDKVALITGGDSGIGRSVAVLFAREGADVAIVHLDESQDADDTKAAVEKEGRKCLVIK 71 Query: 62 LELQDAARCGEAVAQTVARFGRLDGLVNNAG--VNDSVGLDAGRDEFVASLERNLIHYYV 119 +++DA+ C +AV +TV + GRLD L+NNA V+ D + F +L+ NL Y+ Sbjct: 72 GDVKDASFCRKAVEKTVMQLGRLDILINNAAFQVHTRDIEDLTDEHFDETLKTNLYGYFY 131 Query: 120 MAHYCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVN 179 MA +PHLK AI+N S T LTG Y +KG + TR + L G+RVN Sbjct: 132 MAKAAIPHLK-NGSAIINTGSVTGLTGSKELLDYSMTKGGIHAFTRALSGHLVPKGIRVN 190 Query: 180 ALIPAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGR-SSH 238 A+ P V TPL + E ++ S+ P+ KR EE+A VFL S + SS+ Sbjct: 191 AVAPGPVWTPLNPS-----DKEAEDVEKFGSQTPM-KRAAQPEEIAPAYVFLASPQMSSY 244 Query: 239 TTGQWVFVDGGY 250 TG+ + + GGY Sbjct: 245 ITGEILPIVGGY 256