BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0840.p (425 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc5098 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 705 0.0 BMEI1707 [MR] COG4948 L-alanine-DL-glutamate epimerase and relat... 703 0.0 YPO1567 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 680 0.0 SMb21113 [MR] COG4948 L-alanine-DL-glutamate epimerase and relat... 640 0.0 BMEI1712 [MR] COG4948 L-alanine-DL-glutamate epimerase and relat... 111 3e-24 >AGc5098 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 425 Score = 705 bits (1820), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/423 (77%), Positives = 371/423 (87%) Query: 1 MTKITALRVLDVRFPTSQSLDGSDAMNPDPDYSAAYVILDTDNEQLKGHGLTFTIGRGNE 60 MTKIT LRV D+RFPTSQSLDGSDAMNPDPDYSAAYVILDTD +LKGHGLTFTIGRGN+ Sbjct: 1 MTKITDLRVFDLRFPTSQSLDGSDAMNPDPDYSAAYVILDTDEPRLKGHGLTFTIGRGND 60 Query: 61 ICCAAIRAMEHLVVGLELEWIAADMGRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAAW 120 ICC AI AM HLVVG +L + + G++WRH+TSDSQLRWIGPDKGA+HLATGAVVNA W Sbjct: 61 ICCMAIEAMRHLVVGTDLATVTENPGKYWRHLTSDSQLRWIGPDKGAMHLATGAVVNAVW 120 Query: 121 DLWAKAQGKPVWKLVADMSPEELVRTIDFRYITDCITPEEALALLREKEAGKAERLRILE 180 DL AK GKPVW+LVADMSPEE+ +D+RY+TD +T ++ALA+LR EAGK ER+ L+ Sbjct: 121 DLLAKKAGKPVWQLVADMSPEEIADIVDYRYLTDVLTRDDALAILRRAEAGKKERIETLK 180 Query: 181 QEGYPCYTTSAGWLGYDDAKLRRLCQQAIDQGFNHVKLKVGRDLEDDKRRVRIAREVLGP 240 EGY CYTTSAGWLGYDDAKLRRLCQ+AID GFNHVK+KVGRDLEDD RR+ I REV+GP Sbjct: 181 NEGYACYTTSAGWLGYDDAKLRRLCQEAIDAGFNHVKMKVGRDLEDDIRRLTITREVIGP 240 Query: 241 DRSLMIDANQVWEVDQAIAWVNELAFAQPWFIEEPTCPDDVEGHRKIRAGIGAVKVATGE 300 DR LMIDANQVWEVDQAI WVN+LAF++P+FIEEPT PDD+ GHRKIRA IG+VKVATGE Sbjct: 241 DRYLMIDANQVWEVDQAIEWVNKLAFSKPFFIEEPTSPDDIAGHRKIRAAIGSVKVATGE 300 Query: 301 MCQNRVLFKQFIMRDAIDVVQIDSCRLGGVNEILAVMLMAAKYGKVVCPHAGGVGLCEYV 360 MCQNR++FKQFI AIDVVQIDSCR+GG+NE+LAV+L+AAKY V PHAGGVGLCEYV Sbjct: 301 MCQNRIMFKQFIAEGAIDVVQIDSCRMGGLNEVLAVLLIAAKYNLPVWPHAGGVGLCEYV 360 Query: 361 QHLSMIDYLCISGSKEGRVTEYVDHLHEHFVDPCVIRNAAYMPPSLPGFSIEMKPASLEQ 420 QHLSMIDYL +SG+KEGRV EYVDHLHEHF++PC IRNAAYMPP LPGFSIEMKP S+E Sbjct: 361 QHLSMIDYLVVSGTKEGRVIEYVDHLHEHFIEPCDIRNAAYMPPKLPGFSIEMKPESIET 420 Query: 421 YRF 423 Y F Sbjct: 421 YTF 423 >BMEI1707 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Bme Brucella melitensis Length = 425 Score = 703 bits (1814), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/425 (77%), Positives = 372/425 (87%) Query: 1 MTKITALRVLDVRFPTSQSLDGSDAMNPDPDYSAAYVILDTDNEQLKGHGLTFTIGRGNE 60 MTKIT LRV D+RFPTSQSLDGSDAMNPDPDYSAAYVILDTD+E LKGHGLTFTIGRGN+ Sbjct: 1 MTKITDLRVFDLRFPTSQSLDGSDAMNPDPDYSAAYVILDTDDEALKGHGLTFTIGRGND 60 Query: 61 ICCAAIRAMEHLVVGLELEWIAADMGRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAAW 120 ICC AI AM HLVVG L+ I A G+FW H+T DSQLRWIGP+KGA+HLATGAVVNA W Sbjct: 61 ICCHAILAMRHLVVGASLDEIRAAPGKFWHHLTGDSQLRWIGPEKGAMHLATGAVVNAIW 120 Query: 121 DLWAKAQGKPVWKLVADMSPEELVRTIDFRYITDCITPEEALALLREKEAGKAERLRILE 180 DL AK GKPVW+LV+++SPEE+ +D+RY+TD +T +EA+ +LR+ E+GKAER+ LE Sbjct: 121 DLLAKQAGKPVWRLVSELSPEEIADIVDYRYLTDVLTRDEAIEILRKAESGKAERIATLE 180 Query: 181 QEGYPCYTTSAGWLGYDDAKLRRLCQQAIDQGFNHVKLKVGRDLEDDKRRVRIAREVLGP 240 EGY CYTTSAGWLGYDDAKLRRLCQ+AID+GFNHVK+KVG DLEDD RR+ IAREV+GP Sbjct: 181 AEGYACYTTSAGWLGYDDAKLRRLCQEAIDEGFNHVKMKVGHDLEDDIRRLTIAREVIGP 240 Query: 241 DRSLMIDANQVWEVDQAIAWVNELAFAQPWFIEEPTCPDDVEGHRKIRAGIGAVKVATGE 300 DR LMIDANQVWEVDQAI WV LAFA P+FIEEPT PDDV GHRKIRA IG VKVATGE Sbjct: 241 DRYLMIDANQVWEVDQAIEWVRRLAFANPFFIEEPTSPDDVAGHRKIRAAIGPVKVATGE 300 Query: 301 MCQNRVLFKQFIMRDAIDVVQIDSCRLGGVNEILAVMLMAAKYGKVVCPHAGGVGLCEYV 360 MCQNR++FKQFI AIDVVQIDSCR+GG+NE+LAV+LMAAKY V PHAGGVGLCEYV Sbjct: 301 MCQNRIMFKQFIAEGAIDVVQIDSCRMGGLNEVLAVLLMAAKYDLPVWPHAGGVGLCEYV 360 Query: 361 QHLSMIDYLCISGSKEGRVTEYVDHLHEHFVDPCVIRNAAYMPPSLPGFSIEMKPASLEQ 420 QHLSMIDY+ +SG+KEGRV EYVDHLHEHF+DPC+IRNAAYMPPSLPGFSIEMKP S+ Q Sbjct: 361 QHLSMIDYVAVSGTKEGRVIEYVDHLHEHFLDPCIIRNAAYMPPSLPGFSIEMKPQSIAQ 420 Query: 421 YRFRG 425 Y F+G Sbjct: 421 YAFKG 425 >YPO1567 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Ype Yersinia pestis Length = 400 Score = 680 bits (1754), Expect = 0.0, Method: Compositional matrix adjust. Identities = 314/399 (78%), Positives = 354/399 (88%) Query: 26 MNPDPDYSAAYVILDTDNEQLKGHGLTFTIGRGNEICCAAIRAMEHLVVGLELEWIAADM 85 MNPDPDYSAAYVILDTDN L GHGLTFTIGRGNEICC AI A+EHL+VG +L + A+M Sbjct: 1 MNPDPDYSAAYVILDTDNPALSGHGLTFTIGRGNEICCVAIHALEHLIVGRDLASMTANM 60 Query: 86 GRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAAWDLWAKAQGKPVWKLVADMSPEELVR 145 G+FWR TSDSQLRWIGP+KGAIHLATGAV+NA WDLW+KA+GKP+W+LVADMSPEELVR Sbjct: 61 GKFWREFTSDSQLRWIGPEKGAIHLATGAVINAVWDLWSKAEGKPLWRLVADMSPEELVR 120 Query: 146 TIDFRYITDCITPEEALALLREKEAGKAERLRILEQEGYPCYTTSAGWLGYDDAKLRRLC 205 IDFRYITDCITPEEAL LL+++ KA RL L+ EGYPCYTTSAGWLGY D KLRRLC Sbjct: 121 CIDFRYITDCITPEEALTLLQQRAHNKALRLEKLQDEGYPCYTTSAGWLGYPDDKLRRLC 180 Query: 206 QQAIDQGFNHVKLKVGRDLEDDKRRVRIAREVLGPDRSLMIDANQVWEVDQAIAWVNELA 265 Q A+D GF+++KLKVGRDLEDD RRVRI REVLGPDR LMIDANQ+WE ++AI WVN+LA Sbjct: 181 QDAVDAGFDYLKLKVGRDLEDDIRRVRITREVLGPDRKLMIDANQIWETNEAIPWVNQLA 240 Query: 266 FAQPWFIEEPTCPDDVEGHRKIRAGIGAVKVATGEMCQNRVLFKQFIMRDAIDVVQIDSC 325 FA PWFIEEPT PDD+EGHR+IR G+ VKVATGEMCQNR++FKQFIMR+AIDVVQID+C Sbjct: 241 FANPWFIEEPTNPDDIEGHRRIRQGVAPVKVATGEMCQNRIMFKQFIMREAIDVVQIDAC 300 Query: 326 RLGGVNEILAVMLMAAKYGKVVCPHAGGVGLCEYVQHLSMIDYLCISGSKEGRVTEYVDH 385 RLGGVNE+LAVMLMAAKY VCPHAGGVGLCEYVQHL+MIDYLCI+G+ +GRV EYVDH Sbjct: 301 RLGGVNEVLAVMLMAAKYDLPVCPHAGGVGLCEYVQHLAMIDYLCIAGTDKGRVIEYVDH 360 Query: 386 LHEHFVDPCVIRNAAYMPPSLPGFSIEMKPASLEQYRFR 424 LHEHFV PCVI+NAAYMPP PGFSIEM +S+E+YRFR Sbjct: 361 LHEHFVHPCVIKNAAYMPPEQPGFSIEMHQSSIEKYRFR 399 >SMb21113 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Sme Sinorhizobium meliloti Length = 400 Score = 640 bits (1651), Expect = 0.0, Method: Compositional matrix adjust. Identities = 299/400 (74%), Positives = 343/400 (85%) Query: 26 MNPDPDYSAAYVILDTDNEQLKGHGLTFTIGRGNEICCAAIRAMEHLVVGLELEWIAADM 85 MNPDPDYSAAYVILDTD L GHGLTFTIGRGN+ICC AI AM HLVVG ++ I Sbjct: 1 MNPDPDYSAAYVILDTDRPGLAGHGLTFTIGRGNDICCMAIEAMRHLVVGQDISNILKHP 60 Query: 86 GRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAAWDLWAKAQGKPVWKLVADMSPEELVR 145 GRFWRH+TSDSQLRWIGP+KGAIHLATGA+VNA WDL AK GKPVW+LVADM EE+ Sbjct: 61 GRFWRHLTSDSQLRWIGPEKGAIHLATGAIVNAVWDLLAKHAGKPVWRLVADMPAEEIAD 120 Query: 146 TIDFRYITDCITPEEALALLREKEAGKAERLRILEQEGYPCYTTSAGWLGYDDAKLRRLC 205 +D+RY+TD +T ++A+ +LR E GKAER+ LE+EGYPCYTTSAGWLGYDDAKLRRL Sbjct: 121 IVDYRYLTDVLTRDDAVEILRRAEPGKAERIATLEKEGYPCYTTSAGWLGYDDAKLRRLA 180 Query: 206 QQAIDQGFNHVKLKVGRDLEDDKRRVRIAREVLGPDRSLMIDANQVWEVDQAIAWVNELA 265 Q+A+D GF+H+K+KVGRDL+DD RR+RIAREV+GPDR LMIDANQVWEV +AI WV +LA Sbjct: 181 QEAVDAGFDHIKMKVGRDLDDDIRRLRIAREVIGPDRYLMIDANQVWEVGEAIEWVQKLA 240 Query: 266 FAQPWFIEEPTCPDDVEGHRKIRAGIGAVKVATGEMCQNRVLFKQFIMRDAIDVVQIDSC 325 FA+P+FIEEPT PDDV GHRKIR IG VKVATGEMCQNR++FKQFI AID+VQIDSC Sbjct: 241 FAKPFFIEEPTSPDDVAGHRKIREAIGPVKVATGEMCQNRIMFKQFIAEGAIDIVQIDSC 300 Query: 326 RLGGVNEILAVMLMAAKYGKVVCPHAGGVGLCEYVQHLSMIDYLCISGSKEGRVTEYVDH 385 R+GG+NE+LAV+L+AAKYG V PHAGGVGLCEYVQHLSMIDY+ +SG+K+GRV EYVDH Sbjct: 301 RMGGLNEVLAVLLIAAKYGLPVWPHAGGVGLCEYVQHLSMIDYVAVSGTKDGRVIEYVDH 360 Query: 386 LHEHFVDPCVIRNAAYMPPSLPGFSIEMKPASLEQYRFRG 425 LHEHF+DPCVIRNAAYMPP PGFSIEMK S+E YRF G Sbjct: 361 LHEHFLDPCVIRNAAYMPPERPGFSIEMKQQSIEDYRFDG 400 >BMEI1712 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Bme Brucella melitensis Length = 372 Score = 111 bits (278), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 106/377 (28%), Positives = 155/377 (41%), Gaps = 63/377 (16%) Query: 37 VILDTDNEQLKGHGLTFTIGRGNEICCAAIR-AMEHLVVGLELEWIAADMGRFWRHVTSD 95 ++ TD++ G G ++TIG G + + LV+G + + I A WR + Sbjct: 34 IVTITDSDGAVGTGYSYTIGTGGSSVMRLLADHLAPLVIGEDADCIEA----IWRRLEFA 89 Query: 96 SQLRWIGPDKGAIHLATGAVVNAAWDLWAKAQGKPVWKLVADMSPEELVRTIDFRYITDC 155 + IG A A WDL AK Q P+WKL Sbjct: 90 THATTIGAITALALAAID---TALWDLRAKKQNLPLWKL--------------------- 125 Query: 156 ITPEEALALLREKEAGKAERLRILEQEGYPCYTTSAGWLGYDDAKLRRLCQQAIDQGFNH 215 AG A ++ P YTT GWL + L QA +GF Sbjct: 126 --------------AGGA-------RDHCPLYTTEGGWLHIEKEALVEDALQAKAKGFTG 164 Query: 216 VKLKVGRDL-EDDKRRVRIAREVLGPDRSLMIDANQVWEVDQAIAWVNELAFAQPWFIEE 274 K+K+G+ D R+ R +G +M D NQ + VD+AI L +IEE Sbjct: 165 SKIKIGKPHGAQDYARLSAVRAAVGAAFEIMTDCNQGFTVDEAIRRAERLKELDLAWIEE 224 Query: 275 PTCPDDVEGHRKIRAGIGAVKVATGEMCQNRVLFKQFIMRDAIDVVQIDSCRLGGVNEIL 334 P DD++GH ++ A A VA GE + F++++ + A +VQ+D R+GG+ L Sbjct: 225 PLPADDLDGHMRL-ARSTAAPVAVGESIYSIRHFREYMHKGACSIVQVDVARIGGITPWL 283 Query: 335 AVMLMAAKYGKVVCPHAGGVGLCEYVQHLSMIDYLCISGSKEGRVTEYVDHLHEHFVDPC 394 V MA + VCPH L E H+S+ + GR EY+ L + Sbjct: 284 KVAHMAEAFDIPVCPHF----LMEL--HVSL-----VCAVPNGRYVEYIPQLDDLTDKGM 332 Query: 395 VIRNAAYMPPSLPGFSI 411 IR+ + PS PG I Sbjct: 333 EIRDGHAVAPSQPGIGI 349