BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0822.p (382 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20510 [MR] COG4948 L-alanine-DL-glutamate epimerase and relat... 632 0.0 RSc2751 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 496 e-140 dgoA_2 [MR] COG4948 L-alanine-DL-glutamate epimerase and related... 484 e-136 STM3828 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 478 e-134 mll7085 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 245 1e-64 >SMb20510 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Sme Sinorhizobium meliloti Length = 382 Score = 632 bits (1630), Expect = 0.0, Method: Compositional matrix adjust. Identities = 296/382 (77%), Positives = 339/382 (88%) Query: 1 MKITKISTFIVPPRWCFLKIETDEGIVGWGEPIVEGRAHSVAAAVDELADYLIGKDPRNI 60 MKITK++T+IVPPRW FLKIETDEG+VGWGEP+VEGRA +V AAV EL+DYL+GKDP I Sbjct: 1 MKITKLTTYIVPPRWLFLKIETDEGVVGWGEPVVEGRALTVEAAVHELSDYLVGKDPFLI 60 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVPVHQLLGGAVRDSIRVYSWI 120 EDHW VLYRGGFYRGGAIHMSALAGIDQALWDIKGKALG PVH LLGG RD I+VYSWI Sbjct: 61 EDHWNVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGQPVHSLLGGQCRDRIKVYSWI 120 Query: 121 GGDRPADTAAAAKNAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI 180 GGDRP+D A A+ VARGF A+K+NG EE+Q +D+++KI+ + + +R+A+GP+VGI Sbjct: 121 GGDRPSDVANNAREVVARGFKAIKLNGCEEMQIVDTNEKIDKAVETIGLIRDAIGPHVGI 180 Query: 181 GVDFHGRVHKPMAKVLIKELEPYKLMFIEEPVLSENSEALKEIAHLTSTPIALGERLYSR 240 GVDFHGRVH+PMAKVL KELEP+KLMFIEEPVLSEN EAL+EIA+ STPIALGERLYSR Sbjct: 181 GVDFHGRVHRPMAKVLAKELEPFKLMFIEEPVLSENREALREIANHCSTPIALGERLYSR 240 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD 300 WDFK +LS+G+VDIIQPD+SHAGGITE RKIA MAEAYDVALA HCPLGPIALA+CLQVD Sbjct: 241 WDFKSVLSDGFVDIIQPDLSHAGGITECRKIAAMAEAYDVALAPHCPLGPIALAACLQVD 300 Query: 301 AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR 360 A SYNAFIQEQSLGIHYNE+ND+LDYI +K VFAYEDG+V IPQGPGLGIE++E YV +R Sbjct: 301 AVSYNAFIQEQSLGIHYNEANDILDYISNKEVFAYEDGFVSIPQGPGLGIEVDEAYVMER 360 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 A+ GHRWRNP+WRH DGS AEW Sbjct: 361 AKEGHRWRNPVWRHSDGSVAEW 382 >RSc2751 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Rso Ralstonia solanacearum Length = 382 Score = 496 bits (1278), Expect = e-140, Method: Compositional matrix adjust. Identities = 238/382 (62%), Positives = 294/382 (76%) Query: 1 MKITKISTFIVPPRWCFLKIETDEGIVGWGEPIVEGRAHSVAAAVDELADYLIGKDPRNI 60 MKIT+++T+ +PPRW FLKIETDEG+ GWGEP++EGRA +V AAV ELADYL+G+DPR I Sbjct: 1 MKITRLTTYRLPPRWMFLKIETDEGVAGWGEPVIEGRARTVEAAVHELADYLVGQDPRRI 60 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVPVHQLLGGAVRDSIRVYSWI 120 D W LYRGGFYRGG I MSA+AGIDQALWDIKGK LGVPV++LLGG VRD +R YSW+ Sbjct: 61 NDLWQTLYRGGFYRGGPILMSAIAGIDQALWDIKGKVLGVPVYELLGGLVRDRMRTYSWV 120 Query: 121 GGDRPADTAAAAKNAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI 180 GGDRPAD A K A GF K+NG EE+ IDS ++ +A V +R A G V Sbjct: 121 GGDRPADVIAGMKALQAGGFDHFKLNGCEEMGIIDSARAVDAAVARVAEIRAAFGNTVEF 180 Query: 181 GVDFHGRVHKPMAKVLIKELEPYKLMFIEEPVLSENSEALKEIAHLTSTPIALGERLYSR 240 G+DFHGRV PMAKVLI+ LEPY+ +FIEEPVL+E +E+ +A T PIA GER++SR Sbjct: 181 GLDFHGRVSAPMAKVLIRALEPYRPLFIEEPVLAEQAESYARLAAQTHLPIAAGERMFSR 240 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD 300 ++FKR+L G + I+QPD+SHAGGITE KIA MAEAYDVALA HCPLGPIALA+CL +D Sbjct: 241 FEFKRVLEAGGLAILQPDLSHAGGITECLKIAGMAEAYDVALAPHCPLGPIALAACLHID 300 Query: 301 AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR 360 S+NA +QEQS+GIHYN+ +LLDY+R+K FA E GY++ P+ PGLG++I+E V +R Sbjct: 301 FVSWNATLQEQSMGIHYNQGAELLDYVRNKADFALEGGYIRPPRLPGLGVDIDEALVIER 360 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 + WRNP+WRH DGS AEW Sbjct: 361 SRSAPDWRNPVWRHADGSVAEW 382 >dgoA_2 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Eco Escherichia coli K12 Length = 477 Score = 484 bits (1247), Expect = e-136, Method: Compositional matrix adjust. Identities = 230/382 (60%), Positives = 284/382 (74%) Query: 1 MKITKISTFIVPPRWCFLKIETDEGIVGWGEPIVEGRAHSVAAAVDELADYLIGKDPRNI 60 MKITKI+T+ +PPRW FLKIETDEG+VGWGEP++EGRA +V AAV EL DYLIG+DP I Sbjct: 96 MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRI 155 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVPVHQLLGGAVRDSIRVYSWI 120 D W V+YR GFYRGG I MSA+AGIDQALWDIKGK L PV QL+GG VRD I+ YSW+ Sbjct: 156 NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV 215 Query: 121 GGDRPADTAAAAKNAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI 180 GGDRPAD K GF K+NG EEL ID+ ++ + V +REA G + Sbjct: 216 GGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEF 275 Query: 181 GVDFHGRVHKPMAKVLIKELEPYKLMFIEEPVLSENSEALKEIAHLTSTPIALGERLYSR 240 G+DFHGRV PMAKVLIKELEPY+ +FIEEPVL+E +E ++A T P+A GER++SR Sbjct: 276 GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR 335 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD 300 +DFKR+L G + I+QPD+SHAGGITE KIA MAEAYDV LA HCPLGPIALA+CL +D Sbjct: 336 FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHID 395 Query: 301 AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR 360 SYNA +QEQS+GIHYN+ +LLD++++K F+ G+ K PGLG+EI+E V + Sbjct: 396 FVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEF 455 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 ++ WRNP+WRH+D S AEW Sbjct: 456 SKNAPDWRNPLWRHEDNSVAEW 477 >STM3828 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Sty Salmonella typhimurium LT2 Length = 382 Score = 478 bits (1229), Expect = e-134, Method: Compositional matrix adjust. Identities = 228/382 (59%), Positives = 282/382 (73%) Query: 1 MKITKISTFIVPPRWCFLKIETDEGIVGWGEPIVEGRAHSVAAAVDELADYLIGKDPRNI 60 MKIT I+T+ +PPRW FLKIETDEG+VGWGEP++EGRA +V AAV E ADYLIGKDP I Sbjct: 1 MKITHITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHEFADYLIGKDPARI 60 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVPVHQLLGGAVRDSIRVYSWI 120 D W V+YR GFYRGG I MSA+AGIDQALWDIKGK L PV QL+GG VRD I+ YSW+ Sbjct: 61 NDLWQVMYRAGFYRGGPIMMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV 120 Query: 121 GGDRPADTAAAAKNAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI 180 GGDRPAD + GF K+NG EE+ ID+ ++ + V +REA G + Sbjct: 121 GGDRPADVIDGIEKLRGIGFDTFKLNGCEEMGVIDNSRAVDAAVNTVAQIREAFGSEIEF 180 Query: 181 GVDFHGRVHKPMAKVLIKELEPYKLMFIEEPVLSENSEALKEIAHLTSTPIALGERLYSR 240 G+DFHGRV PMAKVLIKELEPY+ +FIEEPVL+E +E +A T PIA GER++SR Sbjct: 181 GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPRLAAQTHIPIAAGERMFSR 240 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD 300 ++FKR+L G + I+QPD+SHAGGITE KIA MAEAYDVALA HCPLGPIALA+CL +D Sbjct: 241 FEFKRVLDAGGLAILQPDLSHAGGITECYKIAGMAEAYDVALAPHCPLGPIALAACLHID 300 Query: 301 AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR 360 S NA QEQS+GIHYN+ +LLD++++K F+ + G+ K PGLG++I+E V + Sbjct: 301 FVSRNAVFQEQSMGIHYNKGAELLDFVKNKEDFSMDGGFFKPLTKPGLGVDIDEARVIEL 360 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 ++ WRNP+WRH DGS AEW Sbjct: 361 SKSAPDWRNPLWRHADGSVAEW 382 >mll7085 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Mlo Mesorhizobium loti Length = 390 Score = 245 bits (626), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 152/399 (38%), Positives = 225/399 (56%), Gaps = 26/399 (6%) Query: 1 MKITKISTFIVPP---RWCFLKIETDE-GIVGWGEPIVEGRAHSVAAAVDELADYLIGKD 56 MKIT I + W F+++ETDE G+ GWGE +E + +V A+ +L LIG+D Sbjct: 1 MKITAIKALVCNAEMRNWVFVRVETDEPGLFGWGEATLEWKTRAVVGALQDLEALLIGQD 60 Query: 57 PRNIEDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVPVHQLLGGAVRDSIRV 116 PRN+E + +L R GF+R G MSA++GI+ ALWDI GK+LGVPV +LLGG VRD +R Sbjct: 61 PRNVEQCYQILTRHGFWRLGVEGMSAVSGIEIALWDILGKSLGVPVWRLLGGQVRDYLRT 120 Query: 117 YSWIG-GDRPADTAAAAKNAVAR--------GFTAVKMNGTEELQFIDSHDKIELTLANV 167 Y+ +G GD A + + + + G+ AVK+ ++ ++ V Sbjct: 121 YTHLGLGDMKAVYESGSADTIVERGRIVTQAGYDAVKVVCIPYTHYVSPAKAVDSVADMV 180 Query: 168 QAVREAVGPNVGIGVDFHGRVHKPMAKV-LIKELEPYKLMFIEEPVLSENSEALKEIAHL 226 A+REAVGP++ I VDFHGR A + +K +EP ++MF EEPV E+ L I Sbjct: 181 GALREAVGPDIDIMVDFHGRPASVNAAMDYLKAIEPARIMFAEEPVPPEDVAGLAHITAR 240 Query: 227 TSTPIALGERLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHC 286 ++ PIA GERL R +F + ++ +I QPD+ H GG+ ET+KIA +AE + LA H Sbjct: 241 SAIPIASGERLIGRREFTQSVAARAFNIAQPDICHTGGLLETKKIAALAETAGIGLAPHN 300 Query: 287 PLGPIALASCLQVDAGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGP 346 PLGPIA + L + N IQE+ G +D++ + D+ + G +P P Sbjct: 301 PLGPIAGVAALHFGISTPNVIIQEEMSG-AVAWYDDVVTWPIDR-----KPGRWDVPDLP 354 Query: 347 GLGIEINEEYVRQ---RAEVGHRWRNPIWRHKDGSFAEW 382 GLG+ +NE+ + + + EV H RN + DG+ +W Sbjct: 355 GLGVTVNEDVIARHPFKQEVLHA-RNAV--MPDGTVVDW 390