BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0774.p (300 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0917 [E] COG0031 Cysteine synthase Rso Ralstonia solanacearum 489 e-138 PA0932 [E] COG0031 Cysteine synthase Pae Pseudomonas aeruginosa 407 e-113 STM2440 [E] COG0031 Cysteine synthase Sty Salmonella typhimurium... 394 e-109 cysM [E] COG0031 Cysteine synthase Eco Escherichia coli K12 391 e-109 ZcysM [E] COG0031 Cysteine synthase EcZ Escherichia coli O157:H7... 385 e-107 >RSc0917 [E] COG0031 Cysteine synthase Rso Ralstonia solanacearum Length = 300 Score = 489 bits (1260), Expect = e-138, Method: Compositional matrix adjust. Identities = 232/300 (77%), Positives = 267/300 (89%) Query: 1 MSYPTIESTIGNTPLIQLPRIGGDEARRRNNLILGKLEGNNPAGSVKDRAAYSMITRAEA 60 M+YPTIE TIGNTPL++L RI G++ RR N+ILGKLEGNNPAGSVKDR A SMI AE Sbjct: 1 MAYPTIEDTIGNTPLVRLQRIPGEDNARRGNVILGKLEGNNPAGSVKDRPALSMIKHAET 60 Query: 61 RGQIKPGDTLIEATSGNTGIALAMVAAMRGYKMVLLMPENLSEERRQSMAAYGAKIVLTP 120 RG+IKPGDTLIEATSGNTGIALAM AA+RGYKMVL+MPE+LS ERRQSMAAYGA+IVLTP Sbjct: 61 RGRIKPGDTLIEATSGNTGIALAMAAAIRGYKMVLIMPEDLSVERRQSMAAYGAEIVLTP 120 Query: 121 KSGGMEYARDLAEQMQKNGEGLILDQFANPDNPLAHYETTGPEIWRDTKGSITHFVSAMG 180 GGMEYARDLA+ M+++G+G+ILDQFANPDNPLAHYE TGPEIWRDT+G ITHFVSAMG Sbjct: 121 VKGGMEYARDLADAMERDGKGVILDQFANPDNPLAHYEGTGPEIWRDTEGRITHFVSAMG 180 Query: 181 TTGTIMGVAQFLKEKNAQVQIVGAQPEEGSSIPGIRKWPEAYLPKIFDRSRVDQIESVSQ 240 TTGTI GV++FLKEKN +QIVGAQP EGS IPGIRKWPEAY+PKI+D +D+ ESVSQ Sbjct: 181 TTGTITGVSRFLKEKNPAIQIVGAQPAEGSRIPGIRKWPEAYMPKIYDPKHIDRTESVSQ 240 Query: 241 ADAENMARKLAVTEGLFCGISAAGACEVAVRLSHQLENATIVFIVCDRGDRYLSTGVFPA 300 +DAE+MAR++A EG+FCGISAAGA VA+R++ +ENATIVF+VCDRGDRYLSTGVFPA Sbjct: 241 SDAEHMARRMAREEGIFCGISAAGALCVALRVAEAVENATIVFVVCDRGDRYLSTGVFPA 300 >PA0932 [E] COG0031 Cysteine synthase Pae Pseudomonas aeruginosa Length = 299 Score = 407 bits (1046), Expect = e-113, Method: Compositional matrix adjust. Identities = 190/298 (63%), Positives = 242/298 (81%), Gaps = 4/298 (1%) Query: 1 MSYPTIESTIGNTPLIQLPRIGGDEARRRNNLILGKLEGNNPAGSVKDRAAYSMITRAEA 60 + YPTI +GNTPL++L R+ G+ + N +L KLEGNNPAGSVKDR A SMITRAE Sbjct: 3 VQYPTIADCVGNTPLVRLQRLPGETS----NTLLVKLEGNNPAGSVKDRPALSMITRAEL 58 Query: 61 RGQIKPGDTLIEATSGNTGIALAMVAAMRGYKMVLLMPENLSEERRQSMAAYGAKIVLTP 120 RG I+PGDTLIEATSGNTGIALAM AA++GYKM+L+MP+N + ER+ +M AYGA+++L Sbjct: 59 RGDIRPGDTLIEATSGNTGIALAMAAAIKGYKMILIMPDNSTAERKAAMTAYGAELILVS 118 Query: 121 KSGGMEYARDLAEQMQKNGEGLILDQFANPDNPLAHYETTGPEIWRDTKGSITHFVSAMG 180 K GME ARDLA+++Q+ G G +LDQFAN DNP AHY +TGPEIW+ T GSITHFVS+MG Sbjct: 119 KEEGMEGARDLADKLQREGRGKVLDQFANGDNPEAHYHSTGPEIWQQTGGSITHFVSSMG 178 Query: 181 TTGTIMGVAQFLKEKNAQVQIVGAQPEEGSSIPGIRKWPEAYLPKIFDRSRVDQIESVSQ 240 TTGTIMGV+++LKE+N VQIVG QP EGS+IPGIR+WP+ YLPKI+D SRVD++ + Q Sbjct: 179 TTGTIMGVSRYLKEQNPAVQIVGLQPMEGSAIPGIRRWPQEYLPKIYDASRVDRVVDMHQ 238 Query: 241 ADAENMARKLAVTEGLFCGISAAGACEVAVRLSHQLENATIVFIVCDRGDRYLSTGVF 298 +AE++ R+LA EG+FCG+S+ GA +RLS +LENA +V I+CDRGDRYLS+GV+ Sbjct: 239 DEAEDIMRRLAREEGIFCGVSSGGAVAAMLRLSRELENAVLVAIICDRGDRYLSSGVY 296 >STM2440 [E] COG0031 Cysteine synthase Sty Salmonella typhimurium LT2 Length = 303 Score = 394 bits (1012), Expect = e-109, Method: Compositional matrix adjust. Identities = 190/294 (64%), Positives = 228/294 (77%), Gaps = 4/294 (1%) Query: 5 TIESTIGNTPLIQLPRIGGDEARRRNNLILGKLEGNNPAGSVKDRAAYSMITRAEARGQI 64 T+E TIGNTPL++L R+G D I KLEGNNPAGSVKDRAA SMI AE RG+I Sbjct: 3 TLEQTIGNTPLVKLQRLGPDNGSE----IWVKLEGNNPAGSVKDRAALSMIVEAEKRGEI 58 Query: 65 KPGDTLIEATSGNTGIALAMVAAMRGYKMVLLMPENLSEERRQSMAAYGAKIVLTPKSGG 124 KPGD LIEATSGNTGIALAM+AA++GY+M LLMP+N+S+ERR +M AYGA+++L K G Sbjct: 59 KPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQG 118 Query: 125 MEYARDLAEQMQKNGEGLILDQFANPDNPLAHYETTGPEIWRDTKGSITHFVSAMGTTGT 184 ME ARDLA M + GEG +LDQF NPDNP AHY TTGPEIWR T G ITHFVS+MGTTGT Sbjct: 119 MEGARDLALAMSERGEGKLLDQFNNPDNPYAHYTTTGPEIWRQTSGRITHFVSSMGTTGT 178 Query: 185 IMGVAQFLKEKNAQVQIVGAQPEEGSSIPGIRKWPEAYLPKIFDRSRVDQIESVSQADAE 244 I GV++FL+E+ V IVG QPEEGSSIPGIR+WP Y+P IF+ S VD++ + Q DAE Sbjct: 179 ITGVSRFLREQEKPVTIVGLQPEEGSSIPGIRRWPAEYMPGIFNASLVDEVLDIHQNDAE 238 Query: 245 NMARKLAVTEGLFCGISAAGACEVAVRLSHQLENATIVFIVCDRGDRYLSTGVF 298 N R+LAV EG+FCG+S+ GA A+R++ A +V I+CDRGDRYLSTGVF Sbjct: 239 NTMRELAVREGIFCGVSSGGAVAGALRVARATPGAIVVAIICDRGDRYLSTGVF 292 >cysM [E] COG0031 Cysteine synthase Eco Escherichia coli K12 Length = 303 Score = 391 bits (1005), Expect = e-109, Method: Compositional matrix adjust. Identities = 188/294 (63%), Positives = 230/294 (78%), Gaps = 4/294 (1%) Query: 5 TIESTIGNTPLIQLPRIGGDEARRRNNLILGKLEGNNPAGSVKDRAAYSMITRAEARGQI 64 T+E TIGNTPL++L R+G D + KLEGNNPAGSVKDRAA SMI AE RG+I Sbjct: 3 TLEQTIGNTPLVKLQRMGPDNGSE----VWLKLEGNNPAGSVKDRAALSMIVEAEKRGEI 58 Query: 65 KPGDTLIEATSGNTGIALAMVAAMRGYKMVLLMPENLSEERRQSMAAYGAKIVLTPKSGG 124 KPGD LIEATSGNTGIALAM+AA++GY+M LLMP+N+S+ERR +M AYGA+++L K G Sbjct: 59 KPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQG 118 Query: 125 MEYARDLAEQMQKNGEGLILDQFANPDNPLAHYETTGPEIWRDTKGSITHFVSAMGTTGT 184 ME ARDLA +M GEG +LDQF NPDNP AHY TTGPEIW+ T G ITHFVS+MGTTGT Sbjct: 119 MEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITHFVSSMGTTGT 178 Query: 185 IMGVAQFLKEKNAQVQIVGAQPEEGSSIPGIRKWPEAYLPKIFDRSRVDQIESVSQADAE 244 I GV++F++E++ V IVG QPEEGSSIPGIR+WP YLP IF+ S VD++ + Q DAE Sbjct: 179 ITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLPGIFNASLVDEVLDIHQRDAE 238 Query: 245 NMARKLAVTEGLFCGISAAGACEVAVRLSHQLENATIVFIVCDRGDRYLSTGVF 298 N R+LAV EG+FCG+S+ GA A+R++ +A +V I+CDRGDRYLSTGVF Sbjct: 239 NTMRELAVREGIFCGVSSGGAVAGALRVAKANPDAVVVAIICDRGDRYLSTGVF 292 >ZcysM [E] COG0031 Cysteine synthase EcZ Escherichia coli O157:H7 EDL933 Length = 303 Score = 385 bits (990), Expect = e-107, Method: Compositional matrix adjust. Identities = 186/294 (63%), Positives = 229/294 (77%), Gaps = 4/294 (1%) Query: 5 TIESTIGNTPLIQLPRIGGDEARRRNNLILGKLEGNNPAGSVKDRAAYSMITRAEARGQI 64 T+E TIGNTPL++L R+G + + KLEGNNPAGSVKDRAA SMI AE RG+I Sbjct: 3 TLEQTIGNTPLVKLQRMGPNNGSE----VWLKLEGNNPAGSVKDRAALSMIVEAEKRGEI 58 Query: 65 KPGDTLIEATSGNTGIALAMVAAMRGYKMVLLMPENLSEERRQSMAAYGAKIVLTPKSGG 124 KPGD LIEATSGNTGIALAM+AA++GY+M LLMP+N+S+ERR +M AYGA+++L K G Sbjct: 59 KPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQG 118 Query: 125 MEYARDLAEQMQKNGEGLILDQFANPDNPLAHYETTGPEIWRDTKGSITHFVSAMGTTGT 184 ME ARDLA +M GEG +LDQF NPDNP AHY TTGPEIW+ T G IT+FVS+MGTTGT Sbjct: 119 MEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITYFVSSMGTTGT 178 Query: 185 IMGVAQFLKEKNAQVQIVGAQPEEGSSIPGIRKWPEAYLPKIFDRSRVDQIESVSQADAE 244 I GV++F++E++ V IVG QPEEGSSIPGIR+WP YLP IF+ S VD++ + Q DAE Sbjct: 179 ITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLPGIFNASLVDEVLDIHQRDAE 238 Query: 245 NMARKLAVTEGLFCGISAAGACEVAVRLSHQLENATIVFIVCDRGDRYLSTGVF 298 N R+LAV EG+FCG+S+ GA A+R++ A +V I+CDRGDRYLSTGVF Sbjct: 239 NTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVVAIICDRGDRYLSTGVF 292