BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0496.p (237 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1158 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 246 3e-65 TM1158 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 149 7e-36 slr0541 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 143 4e-34 MA2966 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 126 5e-29 MA2696 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 125 7e-29 >RSc1158 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Rso Ralstonia solanacearum Length = 242 Score = 246 bits (628), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 140/230 (60%), Positives = 162/230 (70%), Gaps = 3/230 (1%) Query: 1 MKTAIAIRHVHFEDLGILHEVLREQAYALTYLDACIDDLDTPLVQQADLLIVLGGPIGAY 60 MKTA+A+RHV FE LG+L +L + YA+ LDA + L + ADL++VLGGPIGA+ Sbjct: 1 MKTALALRHVPFEHLGVLESLLLGRGYAVRTLDAGLQPLPAEALAAADLVVVLGGPIGAF 60 Query: 61 DDARYPFLASELAAITRRLMRGAPTLGICLGAQLMARALGADVAPMG--VKEIGYAPLSL 118 DD RYPFL E AI RRL P LGICLGAQLMARALGA VAPMG VKEIG+APL+L Sbjct: 61 DDDRYPFLREETDAIARRLAARKPLLGICLGAQLMARALGAAVAPMGDNVKEIGFAPLTL 120 Query: 119 TEAGRHSPLAALED-VPVLHWHGDRFEIPAQATHLAGSALCAQQAFAVGRQVLALQFHLE 177 T G SPLA L D PVLHWHGDRFE+P AT LA +++CA+QAFA+G L LQFHLE Sbjct: 121 TAEGAASPLAPLADGTPVLHWHGDRFELPPGATRLASTSVCAEQAFALGDHALGLQFHLE 180 Query: 178 ADPKRIEQWLVGHACELDHAGIDPRHLRDAAQRLGGTLQQAARHVVTQWL 227 AD + +E WL+GHA EL AGIDPR LR A L L Q A V T WL Sbjct: 181 ADLRELEAWLIGHAAELAAAGIDPRTLRGQAPALAAPLAQRAHDVFTAWL 230 >TM1158 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Tma Thermotoga maritima Length = 227 Score = 149 bits (375), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 81/227 (35%), Positives = 130/227 (57%), Gaps = 11/227 (4%) Query: 4 AIAIRHVHFEDLGILHEVLREQAYALTYLDACI-DDLDTPLVQQADLLIVLGGPIGAYDD 62 +AIRHV EDLG++ ++ RE+ ++ YLD + L+ PL ++ L+++LGG +GAY++ Sbjct: 3 VLAIRHVEIEDLGMMEDIFREKNWSFDYLDTPKGEKLERPL-EEYSLVVLLGGYMGAYEE 61 Query: 63 ARYPFLASELAAITRRLMRGAPTLGICLGAQLMARALGADVAP-MGVKEIGY---APLSL 118 +YPFL E I L + P LGICLG+Q++A+ LGA V +EIG+ +S Sbjct: 62 EKYPFLKYEFQLIEEILKKEIPFLGICLGSQMLAKVLGASVYRGKNGEEIGWYFVEKVSD 121 Query: 119 TEAGRHSPLAALEDVPVLHWHGDRFEIPAQATHLAGSALCAQQAFAVGRQVLALQFHLEA 178 + R P + + V WHGD F++P +AT + S Q F G+ V LQFH+E Sbjct: 122 NKFFREFP----DRLRVFQWHGDTFDLPRRATRVFTSEKYENQGFVYGKAV-GLQFHIEV 176 Query: 179 DPKRIEQWLVGHACELDHAGIDPRHLRDAAQRLGGTLQQAARHVVTQ 225 + +++W+ + EL+ IDPR L + A+R L+ R ++ + Sbjct: 177 GARTMKRWIEAYKDELEKKKIDPRLLLETAEREEKVLKGLLRSLLER 223 >slr0541 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Syn Synechocystis Length = 236 Score = 143 bits (360), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 76/175 (43%), Positives = 98/175 (56%), Gaps = 1/175 (0%) Query: 7 IRHVHFEDLGILHEVLREQAYALTYLDACIDDLDTPLVQQADLLIVLGGPIGAYDDARYP 66 +HV FE+LG + LR + YA++ P + DLLIVLGGP+ D+A+YP Sbjct: 6 FQHVPFENLGAIERWLRAKGYAISQTAFFQPSFSLPSLDAIDLLIVLGGPMSVNDEAQYP 65 Query: 67 FLASELAAITRRLMRGAPTLGICLGAQLMARALGADVAPMGVKEIGYAPLSLTEAGRHS- 125 +L E I + + G P LGICLGAQLMA ALGA V P VKEIG+ P+ E S Sbjct: 66 WLVQEKEFIRQAIAVGKPILGICLGAQLMANALGAKVYPNAVKEIGWFPIMGQEGKSQSG 125 Query: 126 PLAALEDVPVLHWHGDRFEIPAQATHLAGSALCAQQAFAVGRQVLALQFHLEADP 180 + V HWHG+ F++P A +A S C QAF +GR + LQ HLE P Sbjct: 126 SFQFPPSLEVFHWHGETFDLPPGAELIASSQACQHQAFQIGRSAIGLQCHLETTP 180 >MA2966 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Mac Methanosarcina acetivorans str.C2A Length = 234 Score = 126 bits (316), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 68/183 (37%), Positives = 99/183 (54%), Gaps = 4/183 (2%) Query: 6 AIRHVHFEDLGILHEVLREQAYALTYLDACIDDLDTPLVQQADLLIVLGGPIGAYDDARY 65 I+HV+FE G + E + ++ + L+ ++ P ++ DLLIV+GGP+ Y+ +Y Sbjct: 5 CIQHVNFETPGTITEWIAQKNHFLS-TTHLYENESFPKIETFDLLIVMGGPMNIYEYEKY 63 Query: 66 PFLASELAAITRRLMRGAPTLGICLGAQLMARALGADVAPMGVKEIGYAPLSLTEAGR-H 124 +L E A + + + G LGICLGAQL+A L A+V KEIG+ P+S A + Sbjct: 64 TWLREEKAFLEKAITGGKAVLGICLGAQLLADVLKAEVFKNNYKEIGWFPVSAERAEKAE 123 Query: 125 SPL--AALEDVPVLHWHGDRFEIPAQATHLAGSALCAQQAFAVGRQVLALQFHLEADPKR 182 PL ED P HWHGD F +P A L + C Q F +V+ LQFHLE + Sbjct: 124 IPLLEGIPEDFPAFHWHGDTFSLPGGAKRLFENEACKNQGFIYENRVIGLQFHLEMSNQT 183 Query: 183 IEQ 185 I+ Sbjct: 184 IKN 186 >MA2696 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Mac Methanosarcina acetivorans str.C2A Length = 238 Score = 125 bits (314), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 68/182 (37%), Positives = 99/182 (54%), Gaps = 4/182 (2%) Query: 6 AIRHVHFEDLGILHEVLREQAYALTYLDACIDDLDTPLVQQADLLIVLGGPIGAYDDARY 65 ++H LG + E + ++Y L + + P + DLLI++GGP+G YD A Sbjct: 5 VLQHSALNTLGTIEEYAKTRSYPLES-TRFYETKNPPSLDSFDLLIIMGGPMGIYDYAEN 63 Query: 66 PFLASELAAITRRLMRGAPTLGICLGAQLMARALGADVAPMGVKEIGYAPLSLTEAGRHS 125 P+L E + I + + G P LGICLGAQL+A LGA + G +E+G+ P+ + Sbjct: 64 PWLRDEKSFIKQAIDAGKPVLGICLGAQLLANILGARIYENGHREMGWFPVKAVRKEENK 123 Query: 126 P--LAAL-EDVPVLHWHGDRFEIPAQATHLAGSALCAQQAFAVGRQVLALQFHLEADPKR 182 P L L E++ V HWH F++P A HL S C Q F G +V+ALQFH E +R Sbjct: 124 PEFLKGLPEEITVFHWHSRTFDLPEGAVHLFRSEGCKNQGFIYGGRVVALQFHPEVHEER 183 Query: 183 IE 184 +E Sbjct: 184 VE 185