BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0057.p (249 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1158 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 181 2e-45 TM1158 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 139 6e-33 slr0541 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 128 1e-29 MA2696 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 120 3e-27 AF1277 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 117 2e-26 >RSc1158 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Rso Ralstonia solanacearum Length = 242 Score = 181 bits (458), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 105/200 (52%), Positives = 126/200 (63%), Gaps = 3/200 (1%) Query: 1 MKTALALRHVPNEDLGQLAPLLLGNGYQIRYVEVGVDALVDVSPLDADLVIVLGGPVAVY 60 MKTALALRHVP E LG L LLLG GY +R ++ G+ L + ADLV+VLGGP+ + Sbjct: 1 MKTALALRHVPFEHLGVLESLLLGRGYAVRTLDAGLQPLPAEALAAADLVVVLGGPIGAF 60 Query: 61 DSDAYPWLRGEVAWLRARMLEDLPTLGICLGAQLMAAALHARVYP-GSCGKEIGWASLQP 119 D D YP+LR E + R+ P LGICLGAQLMA AL A V P G KEIG+A L Sbjct: 61 DDDRYPFLREETDAIARRLAARKPLLGICLGAQLMARALGAAVAPMGDNVKEIGFAPLTL 120 Query: 120 GQHLADSPWLREVVEQQTPVLHWHGDTFDLPAGARHLAASARYPHQAFAWGRHCLALQFH 179 A SP + TPVLHWHGD F+LP GA LA+++ QAFA G H L LQFH Sbjct: 121 TAEGAASPL--APLADGTPVLHWHGDRFELPPGATRLASTSVCAEQAFALGDHALGLQFH 178 Query: 180 PEVDVRTVEHWLVSHAHEIA 199 E D+R +E WL+ HA E+A Sbjct: 179 LEADLRELEAWLIGHAAELA 198 >TM1158 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Tma Thermotoga maritima Length = 227 Score = 139 bits (350), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 68/199 (34%), Positives = 114/199 (57%), Gaps = 5/199 (2%) Query: 4 ALALRHVPNEDLGQLAPLLLGNGYQIRYVEVGVDALVDVSPLDADLVIVLGGPVAVYDSD 63 LA+RHV EDLG + + + Y++ ++ + LV++LGG + Y+ + Sbjct: 3 VLAIRHVEIEDLGMMEDIFREKNWSFDYLDTPKGEKLERPLEEYSLVVLLGGYMGAYEEE 62 Query: 64 AYPWLRGEVAWLRARMLEDLPTLGICLGAQLMAAALHARVYPGSCGKEIGWASLQPGQHL 123 YP+L+ E + + +++P LGICLG+Q++A L A VY G G+EIGW + + + Sbjct: 63 KYPFLKYEFQLIEEILKKEIPFLGICLGSQMLAKVLGASVYRGKNGEEIGWYFV---EKV 119 Query: 124 ADSPWLREVVEQQTPVLHWHGDTFDLPAGARHLAASARYPHQAFAWGRHCLALQFHPEVD 183 +D+ + RE + + V WHGDTFDLP A + S +Y +Q F +G+ + LQFH EV Sbjct: 120 SDNKFFREFPD-RLRVFQWHGDTFDLPRRATRVFTSEKYENQGFVYGK-AVGLQFHIEVG 177 Query: 184 VRTVEHWLVSHAHEIAHTR 202 RT++ W+ ++ E+ + Sbjct: 178 ARTMKRWIEAYKDELEKKK 196 >slr0541 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Syn Synechocystis Length = 236 Score = 128 bits (321), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 82/195 (42%), Positives = 107/195 (54%), Gaps = 9/195 (4%) Query: 7 LRHVPNEDLGQLAPLLLGNGYQIRYVEVGVDALVDVSPLDA-DLVIVLGGPVAVYDSDAY 65 +HVP E+LG + L GY I + + LDA DL+IVLGGP++V D Y Sbjct: 6 FQHVPFENLGAIERWLRAKGYAISQTAFFQPSF-SLPSLDAIDLLIVLGGPMSVNDEAQY 64 Query: 66 PWLRGEVAWLRARMLEDLPTLGICLGAQLMAAALHARVYPGSCGKEIGWASL--QPGQHL 123 PWL E ++R + P LGICLGAQLMA AL A+VYP + KEIGW + Q G+ Sbjct: 65 PWLVQEKEFIRQAIAVGKPILGICLGAQLMANALGAKVYPNAV-KEIGWFPIMGQEGKSQ 123 Query: 124 ADSPWLREVVEQQTPVLHWHGDTFDLPAGARHLAASARYPHQAFAWGRHCLALQFHPEVD 183 + S +E V HWHG+TFDLP GA +A+S HQAF GR + LQ H E Sbjct: 124 SGSFQFPPSLE----VFHWHGETFDLPPGAELIASSQACQHQAFQIGRSAIGLQCHLETT 179 Query: 184 VRTVEHWLVSHAHEI 198 + + + A E+ Sbjct: 180 PTAAQALVDNCADEL 194 >MA2696 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Mac Methanosarcina acetivorans str.C2A Length = 238 Score = 120 bits (301), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 61/148 (41%), Positives = 88/148 (59%), Gaps = 2/148 (1%) Query: 45 LDA-DLVIVLGGPVAVYDSDAYPWLRGEVAWLRARMLEDLPTLGICLGAQLMAAALHARV 103 LD+ DL+I++GGP+ +YD PWLR E ++++ + P LGICLGAQL+A L AR+ Sbjct: 42 LDSFDLLIIMGGPMGIYDYAENPWLRDEKSFIKQAIDAGKPVLGICLGAQLLANILGARI 101 Query: 104 YPGSCGKEIGWASLQPGQHLADSPWLREVVEQQTPVLHWHGDTFDLPAGARHLAASARYP 163 Y +E+GW ++ + + P + + ++ V HWH TFDLP GA HL S Sbjct: 102 YENGH-REMGWFPVKAVRKEENKPEFLKGLPEEITVFHWHSRTFDLPEGAVHLFRSEGCK 160 Query: 164 HQAFAWGRHCLALQFHPEVDVRTVEHWL 191 +Q F +G +ALQFHPEV VE + Sbjct: 161 NQGFIYGGRVVALQFHPEVHEERVESMI 188 >AF1277 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Afu Archaeoglobus fulgidus Length = 213 Score = 117 bits (294), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 71/186 (38%), Positives = 100/186 (53%), Gaps = 16/186 (8%) Query: 6 ALRHVPNEDLGQLAPLLLGNGYQIRYVEVGVDALVDVSPLDADLVIVLGGPVAVYDSDAY 65 A+++ P E LG L +L G YVE A + D D +++LGGP+ VY+++ Y Sbjct: 5 AIKNHPAEGLGYLEEVLSREGVDYYYVE----AYSETKMADFDAIVILGGPMGVYEAEKY 60 Query: 66 PWLRGEVAWLRARMLEDLPTLGICLGAQLMAAALHARVYPGSCGKEIGWASLQPGQHLAD 125 P+L+ E+ +R E P +GICLGAQL+AA+L RVYP EIGW + + +AD Sbjct: 61 PFLKWEMDLIRKTYREK-PVMGICLGAQLIAASLGGRVYPYIM--EIGWRRV---RRVAD 114 Query: 126 SPWLREVVEQQTPVLHWHGDTFDLPAGARHLAASARYPHQAFAWGRHCLALQFHPEVDVR 185 P E+ V WHGDTFDLP A + +Q F L LQFH E+ + Sbjct: 115 VPLPDEI-----EVFQWHGDTFDLPRNAELIYEGEEVKNQCFI-AEKALGLQFHVEMTLE 168 Query: 186 TVEHWL 191 +E W+ Sbjct: 169 LIEKWV 174