BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0030.p (242 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0448 [R] COG1451 Predicted metal-dependent hydrolase Bme Br... 130 3e-30 CAC0328 [R] COG1451 Predicted metal-dependent hydrolase Cac Clos... 115 6e-26 MA3501 [R] COG1451 Predicted metal-dependent hydrolase Mac Metha... 115 7e-26 RSc0521 [R] COG1451 Predicted metal-dependent hydrolase Rso Rals... 93 5e-19 jhp1425 [R] COG1451 Predicted metal-dependent hydrolase jHp Heli... 92 8e-19 >BMEII0448 [R] COG1451 Predicted metal-dependent hydrolase Bme Brucella melitensis Length = 246 Score = 130 bits (326), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 85/247 (34%), Positives = 125/247 (50%), Gaps = 14/247 (5%) Query: 2 TKHSVIYGDQQIRFSLRYLQRPTRR--VSIHVSPEGAVQVDAPEGTALPEIKQAVRKRAA 59 +HS+++G + F + +R RR V+I V +G V+V AP + VRK+ A Sbjct: 5 AQHSIVFGGETFAFQI---ERTARRKTVAISVGFDG-VRVLAPSDLDDDRVLGIVRKKGA 60 Query: 60 WIWQQLQAVTRRRLHVLPREYVSGETHFYLGRRHMLKVLRAEGGESGVRLWRGRLEIRAV 119 W+ ++ A + +E+VSGET YLGR++ LK++ E RG I V Sbjct: 61 WLLRKQAAYRELEGQPVAKEFVSGETFHYLGRQYRLKLIPDETAVVTRIAARGSTLIAPV 120 Query: 120 RPDA------LTVRKLLEDWYRQRATDVFAKVLLEMAPRLGLRRTMPPVRLLSMRTQWGS 173 P+ VR L WYR A F +A LG+ P V+++ +WGS Sbjct: 121 LPNGHASVQRAAVRNGLRHWYRAHAIQHFPIRARVIAKTLGI--PTPIVKVVDQSKRWGS 178 Query: 174 CSPKGEILLNPSLVKAPRPCIEYVVAHELCHLREHNHSDRFYKHLARALPDWEARKAELD 233 C +G I LN L AP P ++YV+AHE CH+ EHNHS RF++ L +PD+E R LD Sbjct: 179 CDARGRIRLNWRLAMAPMPLVDYVIAHEACHILEHNHSRRFWRSLEAIMPDYEDRLRRLD 238 Query: 234 ELAELLL 240 + + Sbjct: 239 RMGHQFI 245 >CAC0328 [R] COG1451 Predicted metal-dependent hydrolase Cac Clostridium acetobutylicum Length = 259 Score = 115 bits (289), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 69/242 (28%), Positives = 124/242 (51%), Gaps = 27/242 (11%) Query: 5 SVIYGDQQIRFSLRYLQRPTRRVSIHVSPEGAVQVDAPEGTALPEIKQAVRKRAAWIWQQ 64 ++ Y +Q++ FSL +++ + + I V+ + ++V AP A EIK V K+ WI +Q Sbjct: 27 NIEYMNQKVEFSL--VRKERKSIKISVNRDFTIEVIAPFNVAEEEIKNVVYKKMKWIIKQ 84 Query: 65 LQAVTRRRLHVLPREYVSGETHFYLGRRHMLKVLRAEGGESGVRLWRGRLEIRAVRPDAL 124 ++ + +Y G+ + G + L V +G ++ +R++ + + D Sbjct: 85 MKRMENIIDINEEIKYSDGDAFYIFGTPYRLIV--KKGIKNKMRIFGENVYMYIKEKDNY 142 Query: 125 TVRK-LLEDWYRQRAT-----------DVFAKVLLEMAPRLGLRRTMPPVRLLSMRTQWG 172 +K E WY++ A +F ++ E P+L +R+ M+++WG Sbjct: 143 EYKKNYAEKWYKKIAAIELEYLYNEKYKIFDEIYDE-KPKLCIRK---------MKSKWG 192 Query: 173 SCSP-KGEILLNPSLVKAPRPCIEYVVAHELCHLREHNHSDRFYKHLARALPDWEARKAE 231 S SP +++LN LVK P+ CIEYV+ HELCH++ NH FY ++ +PDW+ +K Sbjct: 193 SYSPADNKVVLNTQLVKIPKTCIEYVIIHELCHVKFRNHRKEFYNYVNNFMPDWKLKKES 252 Query: 232 LD 233 LD Sbjct: 253 LD 254 >MA3501 [R] COG1451 Predicted metal-dependent hydrolase Mac Methanosarcina acetivorans str.C2A Length = 245 Score = 115 bits (289), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 72/230 (31%), Positives = 125/230 (54%), Gaps = 13/230 (5%) Query: 13 IRFSLRYLQRPTRRVSIHVSPEGAVQVDAPEGTALPEIKQAVRKRAAWIWQQLQAVTRRR 72 I + + Y +R R+ +I V P+ V+ AP G +L I+ ++K+A W+ ++L R Sbjct: 14 IDYEIVYSKR-RRKAAIVVRPDLKVEFRAPHGLSLEAIRDMLQKKAGWVQEKLDWFEANR 72 Query: 73 LHVLPREYVSGETHFYLGRRHMLKVLRAEGGESGVRLWRGRLEIRAVRPDAL-------T 125 L + Y GE + Y+GR + LK+L +G + + + G E+ + P+A+ Sbjct: 73 LPGQEKRYTEGEIYLYMGREYPLKILAGKGIKKPLASFNG-AELTVLIPEAVPEELRPVL 131 Query: 126 VRKLLEDWYRQRATDVFAKVLLEMAPRLGLRRTMPPV-RLLSMRTQWGSCSPKGEILLNP 184 V+K + D+YR+ A ++L + +L + PP+ ++ + +WGSCS I +N Sbjct: 132 VKKAVWDFYRECAESNVERLLKIYSEKL---KIAPPIFKVKYQKRRWGSCSADNVIRINF 188 Query: 185 SLVKAPRPCIEYVVAHELCHLREHNHSDRFYKHLARALPDWEARKAELDE 234 L+ AP +EYVV HELCH++E NHS RF+K + +P +E + L + Sbjct: 189 QLMMAPPEQLEYVVVHELCHVKEKNHSFRFWKLVGGLMPGYEIHRKSLKK 238 >RSc0521 [R] COG1451 Predicted metal-dependent hydrolase Rso Ralstonia solanacearum Length = 288 Score = 93.2 bits (230), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 72/219 (32%), Positives = 112/219 (51%), Gaps = 15/219 (6%) Query: 19 YLQRPTRRVSIHVSPEGAVQVDAPEGTALPEIKQAVRKRAAWIWQQL----QAVTRRRLH 74 +L+R +RR V + + + AP L EI+ A+ ++ WI+ +L + RR L Sbjct: 64 HLKRSSRRTIGFVVDDRGLSITAPRWVTLAEIEHAIAEKQKWIFAKLAEWHTSAARRVLP 123 Query: 75 VLPREYVSGETHFYLGRRHMLKVLRAEGG---ESGVRLWRGRLEIRAVRPDALTVRKLLE 131 P + G T +LG+ MLK+ G ++ +R L A ++ ++ Sbjct: 124 --PMRWEDGATLPFLGKPVMLKLESPIGALVFDADMRTLHLGLPPTAT---GQQIKDRVQ 178 Query: 132 DWYRQRATDVFAKVLLEMAPRLGLRRTMPPVRLLSMRTQWGSCSPKGEILLNPSLVKAPR 191 W + +A +F + L A RLG+R + L S T+WGSC+ G+I LN LV P Sbjct: 179 GWLQTQARRLFGERLDVYAERLGVRHSA--YALSSAATRWGSCTADGKIRLNWRLVHFPL 236 Query: 192 PCIEYVVAHELCHLREHNHSDRFYKHLARALPDW-EARK 229 I+YVVAHEL HL+E NHS RF+ + P++ EAR+ Sbjct: 237 SLIDYVVAHELAHLKEMNHSPRFWDTVESIFPEFREARE 275 >jhp1425 [R] COG1451 Predicted metal-dependent hydrolase jHp Helicobacter pylori J99 Length = 235 Score = 92.4 bits (228), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 65/211 (30%), Positives = 107/211 (50%), Gaps = 9/211 (4%) Query: 29 IHVS---PEGAVQVDAPEGTALPEIKQAVRKRAAWIWQQLQAVTRRRLHVLPREYVSGET 85 +H+S P+G+V V P ++ ++ KR WI +Q Q + RE + E+ Sbjct: 23 LHISVCPPDGSVHVSCPLALNDESLRLSLIKRLPWIKEQQQNFLNQNRQS-QREMLERES 81 Query: 86 HFYLGRRHMLKVLRAEGGESGVRLWRGRLEIRAVRPDALTVR-KLLEDWYRQRATDVFAK 144 H+ G+R++LK+ ++ + L + + +L R K+LE++YRQ + Sbjct: 82 HYLFGKRYLLKIEHTIKKHFVLQSPKC-LILHVHQKTSLENRLKVLENYYRQVLREKIQT 140 Query: 145 VLLEMAPRLGLRRTMPPVRLLSMRTQWGSCS-PKGEILLNPSLVKAPRPCIEYVVAHELC 203 + + L ++ ++ M+ WGSC+ K +L N L KAPR IEYVV HEL Sbjct: 141 YINQYEKILN--ESIQSFKIQKMKRIWGSCNIAKRTLLFNLELAKAPRKGIEYVVVHELL 198 Query: 204 HLREHNHSDRFYKHLARALPDWEARKAELDE 234 HL+ +H++ F L+ LPDW+ KA L E Sbjct: 199 HLKARHHNEYFRDLLSLYLPDWQRAKASLKE 229