BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4680.p (503 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980306|ref|YP_001355332.1| methylmalonate-semialdehyde deh... 828 0.0 gi|152979948|ref|YP_001354595.1| succinate-semialdehyde dehydrog... 192 4e-50 gi|152980627|ref|YP_001353009.1| bifunctional proline dehydrogen... 144 1e-35 >gi|152980306|ref|YP_001355332.1| methylmalonate-semialdehyde dehydrogenase [Janthinobacterium sp. Marseille] Length = 505 Score = 828 bits (2140), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/499 (80%), Positives = 457/499 (91%), Gaps = 1/499 (0%) Query: 6 IDHYIQGQRSRSESGRSQDVFNPATGEVTAKVALASVAEVHAAVAAASAAAPAWAETAPL 65 I HYI G+ + + GR QDVFNPATGE +A+V LAS A+V A VA A AAAPAWA+TAPL Sbjct: 7 IKHYINGRLTETAGGRQQDVFNPATGEASAQVVLASDADVQAVVATAKAAAPAWADTAPL 66 Query: 66 KRARVLFKFKELIEQHHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQF 125 KRAR+LFKFK+LIE+HHD LA AIT EHGKVFSDAKGEVTRGLE+VEFA G+PQLLKTQF Sbjct: 67 KRARILFKFKDLIEKHHDDLAAAITREHGKVFSDAKGEVTRGLEVVEFACGVPQLLKTQF 126 Query: 126 TDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPSPSL 185 TDNIGGGIDNWNLRQPLGVTAGITPFNFP MVP+WMAP+A+ATGN+F+LKPSERDPSPSL Sbjct: 127 TDNIGGGIDNWNLRQPLGVTAGITPFNFPFMVPMWMAPVALATGNTFILKPSERDPSPSL 186 Query: 186 MLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRK-ATERAG 244 M+AEL ++AGLPDGVFNV+QGDK+AVD LL+HP+V+AVSFVGSTPIAEYIY A + G Sbjct: 187 MVAELLQQAGLPDGVFNVLQGDKLAVDGLLQHPDVKAVSFVGSTPIAEYIYATGAARKGG 246 Query: 245 TFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADRLV 304 ++PLR QALGGAKNHLVVMPDANL+QAVDALIGAAYGSAGERCMAISVAVAVG +AD+LV Sbjct: 247 SYPLRVQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGDIADKLV 306 Query: 305 EALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAQLLVDGRGLKVPGH 364 ALIPRVK+LKVKNGME DAEMGP+V+AAHKAK+EGYI +GV AGA+LLVDGRGLKV GH Sbjct: 307 AALIPRVKSLKVKNGMEADAEMGPVVSAAHKAKIEGYIDAGVKAGAKLLVDGRGLKVAGH 366 Query: 365 EEGFFIGGTLFDHVTPDMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFTAD 424 ++GFF+GG LFDHVTP+M IYQEEIFGPVLCV+RVP+FA+A++LIN HE+GNGVSLFT+D Sbjct: 367 DKGFFLGGCLFDHVTPEMTIYQEEIFGPVLCVLRVPNFAAALELINRHEFGNGVSLFTSD 426 Query: 425 GNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYKSIM 484 GNTA EFSRR++VGMVGINVPIPVPMAWHSFGGWKRS+FGDTHAYGEEGI+FYTRYKSIM Sbjct: 427 GNTAREFSRRVEVGMVGINVPIPVPMAWHSFGGWKRSMFGDTHAYGEEGIKFYTRYKSIM 486 Query: 485 QRWSASIGKGAEFTMPVAK 503 QRW SIGKGAEF MP +K Sbjct: 487 QRWPESIGKGAEFAMPTSK 505 >gi|152979948|ref|YP_001354595.1| succinate-semialdehyde dehydrogenase (NADP+) [Janthinobacterium sp. Marseille] Length = 490 Score = 192 bits (488), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 152/457 (33%), Positives = 226/457 (49%), Gaps = 16/457 (3%) Query: 22 SQDVFNPATGEVTAKVALASVAEVHAAVAAASAAAPAWAETAPLKRARVLFKFKELIEQH 81 S DV NPA G V E A+ AA AA PAWA +RA ++ + +LI + Sbjct: 31 SFDVNNPADGSKITDVPNLGAQETEQAIQAAHAAFPAWAAKTGKERAGIMRIWFDLIIAN 90 Query: 82 HDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQFTDNIGGGIDNWNLRQP 141 D LA +T E GK +++GEV G +E+ + + + ++QP Sbjct: 91 ADDLAALMTAEQGKPLIESRGEVIYGASFIEWFAEEAKRVSGDVMASTWSDKRTVTIKQP 150 Query: 142 LGVTAGITPFNFPV-MVPLWMAPIAIATGNSFVLKPSERDPSPSLMLAELFKRAGLPDGV 200 +GV A ITP+NFP+ M+ +AP AIA G + V+KP+E+ P +L +AEL RAG+P GV Sbjct: 151 IGVCASITPWNFPIAMITRKVAP-AIAAGCTIVIKPAEQTPLSALAIAELAHRAGIPPGV 209 Query: 201 FNVVQGD---KVAV-DALLEHPEVQAVSFVGSTPIAEYIYRKATERAGTFPLRAQALGGA 256 NVV D +AV L P V+ VSF GSTP+ + + L LGG Sbjct: 210 INVVTSDSDQSIAVGKVLCSSPIVRHVSFTGSTPVGRILMEQCAPTVKKIGLE---LGG- 265 Query: 257 KNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADRLVEALIPRVKALKV 316 +V DA+LD AV + + Y +AG+ C+ + S+ D V+ L +KV Sbjct: 266 HAPFIVFDDADLDAAVVGALQSKYRNAGQTCVCTNRFYVHESIYDTFVQKLGAGAAKIKV 325 Query: 317 KNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAQLLVDGRGLKVPGHEEGFFIGGTLFD 376 NG+E GPL+ A KVE ++ V GA+++ G+ + G+F T+ Sbjct: 326 GNGVEQGVLQGPLIDAQAVKKVEQHVSDAVSKGAKVITGGKQHALG----GYFYEPTVLS 381 Query: 377 HVTPDMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFTADGNTAHEFSRRIQ 436 +++PDM I EE FGPV V++ AV N ++G ++ D + +++ Sbjct: 382 NISPDMLIMHEETFGPVAAVIKFKTEDEAVAAANDTDFGLASYFYSRDIGRVWRVAEKLE 441 Query: 437 VGMVGINVPIPVPMAWHSFGGWKRSLFG-DTHAYGEE 472 GMVGIN + + FGG K+S G + YG E Sbjct: 442 YGMVGINTGL-ISNEVAPFGGVKQSGLGREGSKYGIE 477 >gi|152980627|ref|YP_001353009.1| bifunctional proline dehydrogenase /delta-1-pyrroline-5-carboxylate dehydrogenase [Janthinobacterium sp. Marseille] Length = 1242 Score = 144 bits (363), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 119/431 (27%), Positives = 205/431 (47%), Gaps = 32/431 (7%) Query: 24 DVFNPAT-GEVTAKVALASVAEVHAAVAAASAAAPAWAETAPLKRARVLFKFKELIEQHH 82 +V NPA +V V A A + A+ A++ W +RA +L + +L +QH Sbjct: 601 EVRNPANHADVVGTVQQADAAVLELALQHATSGINTWQAKPIAERAAILLQAADLFQQHA 660 Query: 83 DALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQFTDNIGGGIDNWNLRQPL 142 L + E GK +A GE+ ++ + + + Q + + + QPL Sbjct: 661 TELIALLVREAGKTLPNAIGELREAIDFLRYYSA--QAIAS-------------DANQPL 705 Query: 143 GVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPSPSLMLAELFKRAGLPDGVFN 202 G+ A I+P+NFP+ + A+A N + KP+E+ P + +L +AG+P+ Sbjct: 706 GLVACISPWNFPLSIFTGQVSAALAAANVVLAKPAEQTPLIACRAVQLLHQAGVPENALL 765 Query: 203 VVQGDKVAVDA-LLEHPEVQAVSFVGSTPIAEYIYRKATERAGT----FPLRAQALGGAK 257 + G + A L+ P V AV F GST +A+ I RK +R+ + PL A+ G + Sbjct: 766 FLPGSGETIGAQLVTDPRVSAVVFTGSTQVAQSIQRKLVQRSISEGKDIPLIAET--GGQ 823 Query: 258 NHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADRLVEALIPRVKALKVK 317 N ++V A +Q V +I +A+ SAG+RC A+ V +A++ ++ L ++ L++ Sbjct: 824 NTMIVDSSALCEQVVRDVISSAFDSAGQRCSALRVLCLQDDIAEQTLQMLHGAMQELRIG 883 Query: 318 NGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAQLLVDGRGLKVPGHEEGFFIGGTLFDH 377 ++GP++ A +A ++ YI+ AG Q+ R L +P G F+ + + Sbjct: 884 RPDHLATDVGPVIDAEAQAHLQAYIRQAQAAGKQV----RQLPLPV-TNGSFVAPCIIE- 937 Query: 378 VTPDMKIYQEEIFGPVLCVVRV--PDFASAVQLINAHEYGNGVSLFTADGNTAHEFSRRI 435 D+ Q E+FGPVL V+R D + IN+ +G + + T T R Sbjct: 938 -IADINELQREVFGPVLHVLRYRRDDLPQLLMDINSTGFGLTLGIHTRIDETIDYVVRHA 996 Query: 436 QVGMVGINVPI 446 QVG + +N I Sbjct: 997 QVGNIYVNRNI 1007 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.319 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,132,999 Number of extensions: 48363 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 3 Length of query: 503 Length of database: 1,221,021 Length adjustment: 87 Effective length of query: 416 Effective length of database: 899,382 Effective search space: 374142912 Effective search space used: 374142912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)