BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4602.p (407 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982271|ref|YP_001355014.1| Bcr/CflA family drug resistance... 255 3e-69 gi|152982580|ref|YP_001354596.1| major facilitator superfamily d... 148 5e-37 gi|152982698|ref|YP_001352731.1| major facilitator transporter [... 63 3e-11 gi|152980367|ref|YP_001352036.1| major facilitator superfamily d... 62 7e-11 gi|152982589|ref|YP_001352814.1| major facilitator superfamily m... 58 1e-09 >gi|152982271|ref|YP_001355014.1| Bcr/CflA family drug resistance transporter [Janthinobacterium sp. Marseille] Length = 399 Score = 255 bits (652), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 160/379 (42%), Positives = 221/379 (58%), Gaps = 10/379 (2%) Query: 19 VLAALTAMGALAIDMYLPSFTAIAQDLGVGTNLVQLTLASFLIGLAVGQMFYGPLSDRFG 78 VL L+A+G AIDMYLP+ +I Q L V + VQ +L +F I L +GQ+ YGP+SD G Sbjct: 11 VLGLLSAIGPFAIDMYLPALPSIGQTLDVSMSAVQASLMAFFISLGIGQIIYGPISDMVG 70 Query: 79 RKPPLFFGIALYFVCSLLCSFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGSA 138 RKPPL+FG+AL+ VCS+ C+ A +IETLIVLR QGLG SAGMVIPRA+VRD +A Sbjct: 71 RKPPLYFGLALFAVCSVGCAMAPDIETLIVLRFLQGLGASAGMVIPRAVVRDLHTGNDAA 130 Query: 139 KAFSMLMLVFGLAPILAPFIGGLMLVFANWRGIFVVLTLFGLLCMVGTRKYLTETVDVSR 198 + S+LMLVF ++PILAP G L++ WR +F +T+ +L ++ ET + Sbjct: 131 RLMSLLMLVFSVSPILAPLSGSLLIELWGWRSVFWAVTVAAVLALILLATSQAETRPREQ 190 Query: 199 AEPLHLGRTLRQYWGLLRHRQFMSFVLCGGLVQAGMFAYIAGGPFVVIELHGVKPQYFGF 258 + + Y LLR F+ V G + FAY+A F++I+ +G+ P+ + Sbjct: 191 RVDSSVRSAINAYSLLLREPHFLGLVFIGAFGISSFFAYLANSSFILIDHYGLTPRQYSI 250 Query: 259 VFASNAIGLIAASQINARLVVKRSAERVLGKVLWVPAGLALMVALLV--LAGLESLP--- 313 FA+NA I SQ RL + V+ + V A +M ALL L G+E L Sbjct: 251 AFAANAASFIGVSQFTGRLSARFGLVPVVK--VAVTAYALVMTALLAINLIGIERLDVML 308 Query: 314 LLLFGFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMGTLQFGMGVLSGVVMSLWHDG 373 ++LF FGFL G P T +AL+ G AGTA+ALMGTLQF G + L+ DG Sbjct: 309 VMLFVGFGFL---GLVIPTTTVLALEEHGTIAGTASALMGTLQFVTGAAVMAITGLFVDG 365 Query: 374 TALPLVSVMAFCGISALLL 392 TA P+V+ +A C + AL + Sbjct: 366 TARPMVAGIAACALLALFI 384 >gi|152982580|ref|YP_001354596.1| major facilitator superfamily drug (bicyclomycin) resistance translocase [Janthinobacterium sp. Marseille] Length = 422 Score = 148 bits (374), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 91/286 (31%), Positives = 155/286 (54%), Gaps = 12/286 (4%) Query: 19 VLAALTAMGALAIDMYLPSFTAIAQDLGVGTNLVQLTLASFLIGLAVGQMFYGPLSDRFG 78 +LA L+ +G +ID YLP+F I L VQ TL ++L AV +++G LSD FG Sbjct: 25 MLALLSMLGPFSIDTYLPAFPNIQASLDASALEVQQTLTAYLFSFAVMILWHGALSDAFG 84 Query: 79 RKPPLFFGIALYFVCSLLCSFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGSA 138 R+ + +A++ V +L C+ +IE L V R+ QG+ AG+VI RA++RD + Sbjct: 85 RRNIILVSLAVFAVATLACAAVHSIEYLWVFRMLQGISAGAGVVIGRAIIRDLYAGSAAE 144 Query: 139 KAFSMLMLVFGLAPILAPFIGGLMLVFANWRGIFVVLTLFGLLCMVGTRKYLTETVDVSR 198 + S++ ++F +AP +AP +GG ++ +NWR IF+ L + +L ++ K L E++ + Sbjct: 145 RLLSLVTMIFSIAPAIAPILGGWIVKHSNWRNIFLALFAYAVLLLIYCYKRLPESLPPEK 204 Query: 199 AEPLHLGRTLRQYWGLLRHRQFMSFVLCGGLVQ---AGMFAYIAGGPFVVIELHGVKPQY 255 +P + Y + R F L GG V AGMF Y+A P +++ G+ P Sbjct: 205 RQPFNPHFLYHSYKRVFRSPLFH---LKGGTVAFNFAGMFLYVAAAPVFLVQHLGLGPDQ 261 Query: 256 FGFVFASNAIGL----IAASQINARLVVKRSAERVLGKVLWVPAGL 297 FG+ F G+ +AA+++ + + R + ++G V + AG+ Sbjct: 262 FGWQFIPTVAGIFCGALAANRLAGNIPISR--QVLIGFVFLIGAGI 305 >gi|152982698|ref|YP_001352731.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 460 Score = 62.8 bits (151), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 44/154 (28%), Positives = 76/154 (49%), Gaps = 1/154 (0%) Query: 40 AIAQDLGVGTNLVQLTLASFLIGLAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCSF 99 +I + + T L +S +G+ +G G L+D+FGRKP + + + SL C F Sbjct: 53 SIKAEFALTTTQAGLLASSSFLGMFLGAAIAGMLADKFGRKPVFQWSMIFWGAGSLACGF 112 Query: 100 AQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIG 159 A ++ L+V R+ G G I +MV + + A+ K ++L + L I A + Sbjct: 113 ATDVTWLMVFRVVLGFGMGMEFPIGLSMVSEIVPAKSRGKYVAILEGFWPLGFIAAGILA 172 Query: 160 GLMLVFANWRGIFVVLTLFGLLCMVGTRKYLTET 193 L L WRGIF+ L++ + + R+Y+ E+ Sbjct: 173 YLTLPLIGWRGIFIALSIPAVFVFI-VRRYVPES 205 >gi|152980367|ref|YP_001352036.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 478 Score = 62.0 bits (149), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 43/182 (23%), Positives = 87/182 (47%), Gaps = 4/182 (2%) Query: 12 KFPPWLAVLAA-LTAMGALAIDMYLPSFTAIAQDLGVGTNLVQLTLASFLIGLAVGQMFY 70 ++ PW+ +A + + + I+ +PS +A L V ++ ++S+++ LAV Sbjct: 18 RYLPWVVAIALFMEQLDSTIINTAIPS---MAASLHVTPLSLKAVVSSYILALAVSIPIS 74 Query: 71 GPLSDRFGRKPPLFFGIALYFVCSLLCSFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRD 130 G ++DRFG + + L+ S+LC + N+ L+ R+ QG + M + R V Sbjct: 75 GWMADRFGTRRVFSVAVGLFTFSSILCGLSVNVPMLVAARILQGFSAAMMMPVGRLTVIR 134 Query: 131 RMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFANWRGIFVVLTLFGLLCMVGTRKYL 190 A + +++ + P+L P +GGL++ + W+ IF V GL +++ Sbjct: 135 TFPKSELLAAMNFVIIPALIGPLLGPTVGGLIVHWLPWQAIFFVNVPIGLAAQWMIHRHM 194 Query: 191 TE 192 + Sbjct: 195 PD 196 >gi|152982589|ref|YP_001352814.1| major facilitator superfamily multidrug resistance B translocase [Janthinobacterium sp. Marseille] Length = 517 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 49/169 (28%), Positives = 72/169 (42%), Gaps = 4/169 (2%) Query: 37 SFTAIAQDLGVGTNLVQLTLASFLIGLAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLL 96 S AI+ DLGV + SF + A+ G L+ R G+ I L+ + S L Sbjct: 41 SIPAISGDLGVSPQQGTWVITSFSVANAISVPLTGWLTQRIGQVRLFIGSILLFVLASCL 100 Query: 97 CSFAQNIETLIVLRLFQGLGGSAGMVIP--RAMVRDRMGAEGSAKAFSMLMLVFGLAPIL 154 C FA +IE LI R+ QG AG +IP +A++ S A + + +API+ Sbjct: 101 CGFAPSIEILIAARVLQG--AVAGPMIPLSQALLLSSYPPAKSGMALAFWGMTTLVAPIM 158 Query: 155 APFIGGLMLVFANWRGIFVVLTLFGLLCMVGTRKYLTETVDVSRAEPLH 203 P +GG + W IF + GL T E + P+ Sbjct: 159 GPLLGGWISDNYTWPWIFFINIPIGLFAAWATWSIYKERESRTYKLPID 207 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.330 0.144 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 877,572 Number of extensions: 36260 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 13 Length of query: 407 Length of database: 1,221,021 Length adjustment: 85 Effective length of query: 322 Effective length of database: 906,776 Effective search space: 291981872 Effective search space used: 291981872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)