BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4589.p (546 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligas... 118 9e-28 gi|152980721|ref|YP_001352572.1| acetyl-CoA synthetase [Janthino... 81 1e-16 >gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligase [Janthinobacterium sp. Marseille] Length = 560 Score = 118 bits (296), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 152/587 (25%), Positives = 238/587 (40%), Gaps = 124/587 (21%) Query: 29 AVYGQRTAILHGALRQNWDQ----------TYRRTRRLASA----LQQLGVGKNDTVSAM 74 A YG +L + ++ D+ TYR L++ LQ +G+ K V+ M Sbjct: 20 ARYGSLVELLDESFQKYADRNACVCMDKALTYREVDHLSTEVGAWLQSIGLQKGARVALM 79 Query: 75 LPNTPAMVEAHFGVPMAGAVLNALNIRLDAESIVFMLRHGEAKVLLIDSEFAALAQQL-- 132 +PN A V AG V+ +N + L A+ ++I FAA +++ Sbjct: 80 MPNVLQYPIAIAAVLRAGYVIVNVNPLYKPRELEHQLTDSGAEAIIILENFAATLEKVIA 139 Query: 133 RTQLPGLKIVEVFDELGPPAVAGERFGHLEYEALLATGDEHFEWKMPA------------ 180 TQ+ + + A G+ FG L+ ++ H + +PA Sbjct: 140 STQVKHVIV----------ATMGDLFGGLK-GGIVNFVVRHVKKMVPAYSLPQAVPFKEV 188 Query: 181 --------------DEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILE---WDLP- 222 D L YT GTTG KG + HR N ++N+L+ W P Sbjct: 189 LRQAAGMTLQRPALGHQDLAFLQYTGGTTGTAKGAMLTHR----NIIANVLQNEAWISPV 244 Query: 223 -------KHPVYLWTLPMFHCNGWCFPWTVAARAGVNVCLRKF---EPKLVFDLIAE--- 269 K +++ LP++H F T G ++ + P+ + I E Sbjct: 245 LNTLPKDKQLIFVCALPLYHI----FALTTCGMLGTHLGVMNLLIPNPRDIPGFIKELMK 300 Query: 270 HGITHYCAAPIVHAALANAPEGWRAGIRGPVKAMVAGAPPPAAVLAKMEAMQFELTHVYG 329 + + A ++ L N P+ AK++ + +L + G Sbjct: 301 YKVHFLPAVNTLYNGLLNNPD-----------------------FAKVDFSELKLCNGGG 337 Query: 330 LT----------EVYGPAAVCAEQDAWSALSVDQRAVAKSRQGVRYHLQSGVTVLSPDTM 379 + EV G CA + + A A + G+ V S + + Sbjct: 338 MAVQKVVNDKWREVTG----CAICEGYGLSETSPTATANPPTTNEFTGTIGLPVSSTE-L 392 Query: 380 EPVAADGEEI-----GEIMFRGNICMKGYLKNDRATHEAF-AGGWFHTGDLGVMNADGYI 433 + + +D E+ GEI RG M GY N T ++ A G+F TGD+G+M+ G+ Sbjct: 393 KIIDSDDNEVPHGSAGEIAIRGPQVMAGYWNNPVETAQSMTADGFFKTGDIGIMDERGFT 452 Query: 434 KIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKEGAE 493 KI DR KD+I+ G N+ E+E V+ HP VL A + PDE GE FV ++ A Sbjct: 453 KIVDRKKDMILVSGFNVYPSEIEGVVAAHPGVLECACIGVPDEHTGEAVKLFVVRRDPAL 512 Query: 494 TGAVELTEFCRSHLAGFKVPKAIYF-GPLPKTSTGKIQKFELRKRMK 539 T A EL +CR G+K PK+I F LPK++ GKI + ELR K Sbjct: 513 TEA-ELMAYCREQFTGYKKPKSIEFRTELPKSNVGKILRRELRDEKK 558 >gi|152980721|ref|YP_001352572.1| acetyl-CoA synthetase [Janthinobacterium sp. Marseille] Length = 664 Score = 81.3 bits (199), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 138/561 (24%), Positives = 219/561 (39%), Gaps = 97/561 (17%) Query: 43 RQNWDQTYRRTRRLASALQQLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRL 102 R + + Y R + A+ L++LG+ K D V +P + V A GA + + Sbjct: 117 RVTYKELYERVCKFANGLKELGIQKGDRVIIYMPMSIEGVAAMQACARIGATHSVVFGGF 176 Query: 103 DAESIV-FMLRHGEAKVLLIDSEFAALAQQLR--TQLPGLKIVEV------FDELGPPAV 153 A+S+ ++ G VL D Q+R LP IV+ D + V Sbjct: 177 SAKSLQERIIDAGAVAVLTSD-------YQVRGGKNLPLKSIVDEALALGGCDTIRNVVV 229 Query: 154 AGERFGHLEYEA--------LLATGDEHFEWKMPADEWDAIALNYTSGTTGDPKGVVYHH 205 G + + A L+ E +P + + YTSG+TG PKGV H Sbjct: 230 YKRTGGEMNFVAGRDIWLHDLVDKQKAECE-PVPVGAEHPLFILYTSGSTGKPKGV-QHS 287 Query: 206 RGAALNAVSNILEW--DLPKHPVYLWTLPMFHCNGWCFPWTVAARAGVNVCLRKFEPKL- 262 G L ++W D+ + VY T + G + G + + P Sbjct: 288 SGGYLLWAMLTMKWSFDIKPNDVYWCTADIGWITGHTYVTYGPLACGATQIVFEGVPMFP 347 Query: 263 ----VFDLIAEHGITHYCAAPIVHAALANAPEGWRAGIRGPVKAMVAGAPPPAAVLAKME 318 +D++ +H +T + AP IR +KA A A P + Sbjct: 348 NAGRFWDMVQKHKVTIFYTAP--------------TAIRSLIKA--ADADP------AVH 385 Query: 319 AMQFELTHVYGLTEVYGPAAVCAEQDAWSALSVDQRAVAKSRQGVRYHLQSGVTVLSP-- 376 +++L+ + L V P A + + ++ +A + + ++G V+SP Sbjct: 386 PSKYDLSSLRLLGSVGEPINPEAWMWYYKNIGGERCPIADTF----WQTETGGHVISPMP 441 Query: 377 --DTMEP----------VAADGEEIGEIMFRGN---ICMK----GYLKNDRATHEAFAGG 417 M P AA +E+G + G + +K ++N E F Sbjct: 442 GATPMVPGSCTLPLPGIAAAVVDEVGHDLANGKGGLLVIKRPWPSMIRNIWNDPERFQKS 501 Query: 418 WFH---------TGDLGVMNAD-GYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLA 467 +F GD + N + GY I R D++ G + ++E+E L +P V Sbjct: 502 YFPHELGGTIYLAGDGAIRNEETGYFTITGRIDDVLNISGHRMGTMEIESALVANPIVAE 561 Query: 468 AAVVAQPDEKWGETPCAFVELKEGAETG------AVELTEFCRSHLAGFKVPKAIYFGP- 520 AAVV +PDE GE+ CAFV LK TG A EL ++ + PK I FG Sbjct: 562 AAVVGKPDETTGESICAFVVLKRSRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDN 621 Query: 521 LPKTSTGKIQKFELRKRMKSD 541 LPKT +GKI + LR K + Sbjct: 622 LPKTRSGKIMRRLLRVLAKDE 642 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.320 0.135 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,259,732 Number of extensions: 51758 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 2 Length of query: 546 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 458 Effective length of database: 895,685 Effective search space: 410223730 Effective search space used: 410223730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)