BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4499.p (406 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981192|ref|YP_001353300.1| major facilitator transporter [... 129 3e-31 gi|152981201|ref|YP_001353559.1| major facilitator superfamily d... 78 8e-16 gi|152981427|ref|YP_001351784.1| major facilitator transporter [... 67 2e-12 gi|152981547|ref|YP_001354871.1| major facilitator transporter [... 62 4e-11 >gi|152981192|ref|YP_001353300.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 390 Score = 129 bits (324), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 105/355 (29%), Positives = 186/355 (52%), Gaps = 15/355 (4%) Query: 28 LALGSFAIGTEGFMIAPLLPRMAADFGMSVSAVASLVIVFTLVLALSSPITTVLSARINR 87 LAL +FAIGT F+I LLP +A +S+ + L+ + L +A+ +PI V +A + R Sbjct: 8 LALSAFAIGTTEFVIMGLLPDVARSLSVSIPSAGWLISGYALGVAIGAPIMAVATASLPR 67 Query: 88 RNLLIGAMGIFTLANLLAAHSTGFASLLSSRLLMAVAAGLYTPNANALAGAIVKPEQRGR 147 + L+ MGIF + N+L A +T + L+ +R++ A+ G + + +A ++V ++ Sbjct: 68 KRALLLLMGIFIVGNVLCAVATNYNFLMVARIITALCHGAFFGIGSVVAASLVPENRKAS 127 Query: 148 ALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFLAVAVMGAVAVAGIW--LGVDRRAGGQ 205 A+A++ G+T+A LG+PLG+ +G GW+ F AVAV+G +++ G+W + V+R Sbjct: 128 AVALMFTGLTLANVLGVPLGTALGQVAGWQMPFWAVAVLGVLSLIGLWRVMPVNRHE--- 184 Query: 206 LHVAGLRERLGVIGQSQIRRLLAVTLFWSVGAYAAYPYIAPYLSRTLAFGEAGIGMTVSL 265 LR +G + + + L+ T+ +S +A + Y+AP L G+ T+ L Sbjct: 185 -EKTDLRAEIGQLRNAGLWAALSTTMLFSAATFALFTYVAPLLEDVTHLSPHGVTWTLFL 243 Query: 266 WGAAAAVGVTLGGTLND-RFGSRRVVRAALLLLMLAFWALAAATLLGPAQALVPVLAAVA 324 G VG +GG L D R G+ AL + + A+ ++ + + +L+PV + Sbjct: 244 IGLGLTVGNYIGGRLADWRLGT------ALTGIFMVL-AVVSSLVRWTSPSLIPVEINLF 296 Query: 325 VWGFAVWAFFPAQMARLIAAGTPAQASVALSLNTSTMYLGFSLGSAVGAGILGLG 379 +W +A P ++ G A VA +LN +G +LG+ VG ++ G Sbjct: 297 LWAMVSFAAVPGLQVNVMIFGKAAPNLVA-TLNIGAFNVGNALGAWVGGSVISAG 350 >gi|152981201|ref|YP_001353559.1| major facilitator superfamily drug efflux antiporter [Janthinobacterium sp. Marseille] Length = 408 Score = 78.2 bits (191), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 100/358 (27%), Positives = 163/358 (45%), Gaps = 12/358 (3%) Query: 28 LALGSFAIGTEGFMIAPLLPRMAADFGMSVSAVASLVIVFTLVLALSSPITTVLSARINR 87 LALG F + T F+ A LL MA D G+S A V LV A ++ + VL+ +R Sbjct: 36 LALGVFGLVTAEFLPASLLTAMANDLGVSDGAAGQAVTATALVGAGAALMIPVLTRNFDR 95 Query: 88 RNLLIGAMGIFTLANLLAAHSTGFASLLSSRLLMAVAAGLYTPNANALAGAIVKPEQRGR 147 + +++ + L+N+LAA + LL SR+L+ +A G + A ALA +V R Sbjct: 96 KRVILTLTILLVLSNVLAATAENLPVLLVSRVLLGIALGGFWSMAAALAMRLVPDNLFAR 155 Query: 148 ALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFLAVAVMGAVAVAGIWLGVDRRAGGQLH 207 A++ + G+++A P+G+ +G +GWR+ F+A ++ + + + + Sbjct: 156 AMSFILTGVSVATVGAAPIGAYMGDLWGWRSAFIAAGIVSIITLVVQFFAIPSLPPKD-- 213 Query: 208 VAGLRERLGVIGQSQIRRLLAVTLFWSVGAYAAYPYIAPYLSRTLAFGEAGIGMTVSLWG 267 L+ ++ ++ +R L L G +A + YI P + + I + +G Sbjct: 214 NPSLKILFELLRRTNVRVALFAVLLVISGHFAGFTYIRPLMEHITHLSVSAISAVLLGYG 273 Query: 268 AAAAVGVTLGGTLNDRFGSRRVVRAALLLLMLAFWALAAATLLGPAQALVPVLAAVAVWG 327 G GG + +R VV L+ AL AATL + A+A+WG Sbjct: 274 IGGFFGNFAGGFIAERSERYAVVFGGTLI------ALLAATLWIAGSSATVAAIAIALWG 327 Query: 328 FAVWAFFPAQMARLIAAGTPAQASVALSLNTSTMYLGFSLGSAVGAGIL--GLGVVGG 383 FA A FP I P QA A L + + + G A+G GIL +G +GG Sbjct: 328 FAFGA-FPVGFQTWIVRAAPDQAEAAGGLMVAAFQIAIASG-AIGGGILVDHVGALGG 383 >gi|152981427|ref|YP_001351784.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 384 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 74/262 (28%), Positives = 130/262 (49%), Gaps = 10/262 (3%) Query: 28 LALGSFAIGTEGFMIAPLLPRMAADFGMSVSAVASLVIVFTLVLALSSPITTV--LSARI 85 L L F++ T FM+A ++P + FG+SV+ + L+ + + + + P+ TV L R+ Sbjct: 8 LGLTIFSLTTSEFMVAGMMPSLTQAFGVSVTQIGYLISFYAIGMVIGGPVLTVGLLKLRV 67 Query: 86 NRRNLLIGAMGIFTLANLLAAHSTGFASLLSSRLLMAVAAGLYTPNANALAGAIVKPEQR 145 + + L+ + ++ LA +AA S + + +R + VAA + A+ G +V R Sbjct: 68 SNKKALLCLLLLYALAQAVAAASNSYDIMAGARFVTGVAAAACFGASLAICGEVVAVGSR 127 Query: 146 GRALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFLAVAVMGAVAVAGIWLGVDRRAGGQ 205 RA +IV GG+ +A +G+P +++ +GWRA+F V ++ AV I V R Sbjct: 128 ARAASIVVGGLMLANVVGVPAATLIDQYWGWRASFWLVVMLVAVCSLLIIFMVPRS---- 183 Query: 206 LHVAGLRERLGVIGQSQIRRLLAVTLFWSV---GAYAAYPYIAPYLSRTLAFGEAGIGMT 262 H A + + + L A L + A+AA+ Y AP L+ FG A I Sbjct: 184 -HAAAAPSLDAELAEFKKPALWAAYLTSGLIIGAAFAAFSYFAPILTEVTGFGAAAIPWL 242 Query: 263 VSLWGAAAAVGVTLGGTLNDRF 284 ++L+G A +G + G D++ Sbjct: 243 LALYGVANVIGNMVIGRYADKY 264 >gi|152981547|ref|YP_001354871.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 417 Score = 62.4 bits (150), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 72/292 (24%), Positives = 124/292 (42%), Gaps = 15/292 (5%) Query: 9 TSSTPQAQAHQGSLLTAGFLALGSFAIGTEGFMIAPLLPRMAADFGMSVSAVASLVIVFT 68 ++ TP + + LLT +A F + ++ PL P++ + L+ +T Sbjct: 4 STHTPVIKNERLFLLT---IAGIQFTHILDFMIMMPLGPQLIRVLHIDTHQFGLLLSSYT 60 Query: 69 LVLALSSPITTVLSARINRRNLLIGAMGIFTLANLLAAHSTGFASLLSSRLLMAVAAGLY 128 A SS + R +RR L++ F +A L + F LL +R L G+ Sbjct: 61 FTAAASSLLAATYIDRFDRRKLMLVLYACFIIATLCCGLAPNFTFLLIARALAGAFGGIL 120 Query: 129 TPNANALAGAIVKPEQRGRALAIVSGGMTIAIALGLPLGSVVGHAF---GWRATFLAVAV 185 + ++ E+RG+AL V +I+ G+PLG ++ F GWRA F + + Sbjct: 121 GGLVQTMVADVIPFERRGKALGTVMAAFSISTVAGVPLGLLLASTFTTLGWRAPFFFIVL 180 Query: 186 M-GAVAVAGIWLGVDRRAGGQLH---VAGLRERLGVIGQSQIRRLLAVTLFWSV--GAYA 239 + G V G L + A LH + + +R+ + + R L A + + Sbjct: 181 LAGIFWVIGYKLLPNMTA--HLHNKPLGNVFQRIFAVAREP-RHLTAFAFIAMIMMAGFT 237 Query: 240 AYPYIAPYLSRTLAFGEAGIGMTVSLWGAAAAVGVTLGGTLNDRFGSRRVVR 291 PYIA Y++ + E+ I + GAA L G + D++G +V R Sbjct: 238 VIPYIALYMTTNVGLPESFITVVYLCGGAATFFTSQLIGRMADKYGKLKVYR 289 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.325 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 650,687 Number of extensions: 22214 Number of successful extensions: 218 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 18 Length of query: 406 Length of database: 1,221,021 Length adjustment: 85 Effective length of query: 321 Effective length of database: 906,776 Effective search space: 291075096 Effective search space used: 291075096 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)