BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4498.p (252 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980201|ref|YP_001352726.1| short-chain dehydrogenase/reduc... 189 1e-49 gi|152982225|ref|YP_001354089.1| short chain dehydrogenase [Jant... 83 1e-17 gi|152981275|ref|YP_001354152.1| short-chain alcohol dehydrogena... 76 3e-15 gi|152980322|ref|YP_001353173.1| 3-hydroxybutyrate dehydrogenase... 70 1e-13 gi|152982900|ref|YP_001352586.1| short chain dehydrogenase [Jant... 69 2e-13 >gi|152980201|ref|YP_001352726.1| short-chain dehydrogenase/reductase SDR [Janthinobacterium sp. Marseille] Length = 251 Score = 189 bits (480), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 102/216 (47%), Positives = 142/216 (65%), Gaps = 7/216 (3%) Query: 1 MKLTDNTLFITGGTSGIGRALAEAFHKLGNQVIIGGRRKALLDEVTAANPGMDAIEFDVS 60 MK+T NT+ I+GGTSGIGRALAEAF+ GN+VII GRR+ LLDE+TAA PG+ ++ DV+ Sbjct: 1 MKITGNTILISGGTSGIGRALAEAFYDRGNRVIITGRRQVLLDEITAARPGLVGLQLDVN 60 Query: 61 SLASIDAAAATLKARYPKLNVLINNAGIMPFDDAGSTIDDQVSRN---ILDTNLLGPIRL 117 +S+ + L ++P+LNVLI NAGI +D S D + N I+ TN+LG +R+ Sbjct: 61 DASSLSRFSVQLHEQFPQLNVLIANAGISHAEDMAS--DSWTTDNAEAIVQTNILGVLRM 118 Query: 118 TSALIELLKAQPSATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTHVRVQ 177 + + LLK +P AT + +S LA+VP A VY ASKA LHS+ S R +RH + V Sbjct: 119 AAVALPLLKHKPDATFMATSSALAFVPRADFPVYCASKAFLHSWLQSLRHQMRHFPIEVL 178 Query: 178 EIAPPWVDTDLV--KKSGDPRAMPLEAFIAETMSKL 211 E++PP+V T L ++ DPRAMPL ++ E + L Sbjct: 179 ELSPPYVQTALTGGQQLHDPRAMPLSDYMTEVLQML 214 >gi|152982225|ref|YP_001354089.1| short chain dehydrogenase [Janthinobacterium sp. Marseille] Length = 239 Score = 83.2 bits (204), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 60/191 (31%), Positives = 99/191 (51%), Gaps = 8/191 (4%) Query: 1 MKLTDNTLFITGGTSGIGRALAEAFHKLG-NQVIIGGRRKALLDEVTAANPGMDAIEFDV 59 M + ++ +TG GIG AL A K ++V R + L + A + ++ DV Sbjct: 1 MNMQGKSVLVTGANRGIGAALVRALLKQDVSKVYAAARNPSALPDFGDAR--VVPLQIDV 58 Query: 60 SSLASIDAAAATLKARYPKLNVLINNAGIMPFDDAGSTIDDQVSRNILDTNLLGPIRLTS 119 ++ + AAA + + L+VLINNAGIM F S ++++ + +D N G +R+T Sbjct: 59 TNPDEVKAAAGKISS----LDVLINNAGIMTFAHVLSATPEELAAD-MDVNYYGTLRVTQ 113 Query: 120 ALIELLKAQPSATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTHVRVQEI 179 A + L++ Q I++ S+ +A + YSASKAAL S + R L+ ++ V + Sbjct: 114 AFLPLIEKQGGGAIVNLVSIAGLASVAAISGYSASKAALFSATQAMRIALKAKNIEVIGV 173 Query: 180 APPWVDTDLVK 190 P +DTDL K Sbjct: 174 FPGPIDTDLAK 184 >gi|152981275|ref|YP_001354152.1| short-chain alcohol dehydrogenase [Janthinobacterium sp. Marseille] Length = 248 Score = 75.9 bits (185), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 55/175 (31%), Positives = 87/175 (49%), Gaps = 1/175 (0%) Query: 8 LFITGGTSGIGRALAEAFHKLGNQVIIGGRRKALLDEVTAA-NPGMDAIEFDVSSLASID 66 + +TG TSG G +A F + G++VI GRR+ LD + A P + + DV+ SI Sbjct: 3 VLVTGATSGFGAEMARKFAQHGHKVIATGRRQDRLDALAAEIGPSVLTVAMDVTDKESIA 62 Query: 67 AAAATLKARYPKLNVLINNAGIMPFDDAGSTIDDQVSRNILDTNLLGPIRLTSALIELLK 126 AA A L + +++VLINNAG+ + + +++TN G + +T L+ + Sbjct: 63 AALAGLPPEWKEIDVLINNAGLALGVAPAQSASLEQWETMIETNCKGLVTVTRLLLPEML 122 Query: 127 AQPSATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTHVRVQEIAP 181 + TII+ +S VY A+KA + + + R L T VR IAP Sbjct: 123 KRNRGTIINISSSAGAYAYPGANVYGATKAFVDQFTRNLRADLVGTGVRASNIAP 177 >gi|152980322|ref|YP_001353173.1| 3-hydroxybutyrate dehydrogenase [Janthinobacterium sp. Marseille] Length = 264 Score = 70.5 bits (171), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 58/204 (28%), Positives = 92/204 (45%), Gaps = 13/204 (6%) Query: 3 LTDNTLFITGGTSGIGRALAEAFHKLGNQVIIGGRRKA----LLDEVTAANPGMDAIEF- 57 L T +TG TSGIG +A+A + G +++ G A L N G+ + Sbjct: 6 LHGKTALVTGSTSGIGLGIAQALAQQGADIVLNGFGDANEIKTLQAALMRNYGVKCVHHP 65 Query: 58 -DVSSLASIDAAAATLKARYPKLNVLINNAGIMPFDDAGSTIDD---QVSRNILDTNLLG 113 D+S A I+A A ++ ++++L+NNAGI + TI+D + I+ NL Sbjct: 66 ADMSKPAEIEAMMAFAAQQFGRVDILVNNAGIQHTE----TIEDFPVEKWDAIIAINLSS 121 Query: 114 PIRLTSALIELLKAQPSATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTH 173 T + +++A+ II+ SV V + Y A+K + + HT Sbjct: 122 AFHTTRLALPMMQAKNWGRIINIASVHGLVGSVQKSAYVAAKHGIVGLTKVTALEMAHTG 181 Query: 174 VRVQEIAPPWVDTDLVKKSGDPRA 197 + I P WV T LV+K D RA Sbjct: 182 ITCNAICPGWVLTPLVQKQVDARA 205 >gi|152982900|ref|YP_001352586.1| short chain dehydrogenase [Janthinobacterium sp. Marseille] Length = 278 Score = 69.3 bits (168), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 60/192 (31%), Positives = 96/192 (50%), Gaps = 10/192 (5%) Query: 7 TLFITGGTSGIGRALAEAFHKLGNQVIIGGRRK----ALLDEVTAANPGMDAIEFDVSSL 62 T+ ITG +GIGRA A AF + G +V + GRR+ AL E+ A + + DV Sbjct: 34 TVLITGALTGIGRATAFAFAREGYRVAVSGRREEAGNALAAELRALGNEAEFLLADVRFE 93 Query: 63 ASIDAAAATLKARYPKLNVLINNAGIMPFDDAGSTIDDQVSRNILDT---NLLGPIRLTS 119 A + R+ +++V +NNAG + + I +Q + N +T N+LG + Sbjct: 94 ADVRNLVEQTVVRFGRIDVAVNNAGT---EGQLAPIVEQSAANYAETFGVNVLGTMLSLK 150 Query: 120 ALIELLKAQPSATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRHTHVRVQEI 179 + ++ AQ S +II+ +SV V +A +VY ASK A+ S VRV + Sbjct: 151 HEMRVMLAQGSGSIINLSSVAGKVGIAGASVYVASKHAVEGLTKSAALEGAAAGVRVNAV 210 Query: 180 APPWVDTDLVKK 191 AP V T+++ + Sbjct: 211 APGPVATEMLDR 222 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.317 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 572,016 Number of extensions: 22546 Number of successful extensions: 141 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 19 Length of query: 252 Length of database: 1,221,021 Length adjustment: 81 Effective length of query: 171 Effective length of database: 921,564 Effective search space: 157587444 Effective search space used: 157587444 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)