BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4389.p (313 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Ja... 139 2e-34 gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid... 121 5e-29 gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthino... 118 5e-28 gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthi... 100 2e-22 >gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 327 Score = 139 bits (351), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 89/262 (33%), Positives = 138/262 (52%), Gaps = 11/262 (4%) Query: 60 VSARVMDASPALRVISKHGTGIDTIDSQAAQQRGIVVKAAAGANAPAVAEHTWALIMACA 119 +SA V+ A+P L+ I G + ID AA G++V A++ W L+MA A Sbjct: 57 MSADVIAANPQLKAICNVAVGYNNIDVAAATAAGVMVTNTPDVLNETTADYAWTLLMANA 116 Query: 120 KNVVGLDRRMRDGHWDKSTHKSL---ELKGRTLGLVGLGAIGRRVAAVAVALEMPVLAYD 176 + V + +R G+W S ++ G TLG++G+G IG+ +A + +M V+ ++ Sbjct: 117 RRVCESEHWLRAGNWKHWRFDSFLGADIHGATLGILGMGRIGQAIARRSTGFDMNVIYHN 176 Query: 177 -----PYAKEAPQGVQLVDLDTLLAGSDVVSLHCPLTAENKHMINAQSLARMKDGAILVN 231 P + + V + LL +D + L P + + H+I A LA MK A LVN Sbjct: 177 RSRATPEQEAYANNARYVSKEELLRSADHLILVLPYSPQTHHIIGAAELALMKPTATLVN 236 Query: 232 TARGGLIDEQALIAALGSGKLLAAGLDSFEKEPFAAPHP-LQQVGNAILSPHIGGVTTDA 290 ARGG++D+ ALIAAL ++ +AGLD FE EP A HP + N +L+PHIG + Sbjct: 237 IARGGIVDDVALIAALRENRIASAGLDVFENEP--ALHPDFLTLSNVVLTPHIGSGSEKT 294 Query: 291 YIAMGTGAASNVLAVLNTQAQP 312 AM A++N+ A ++ Q P Sbjct: 295 RRAMADCASANLAAAMSGQQPP 316 >gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 323 Score = 121 bits (304), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 86/265 (32%), Positives = 127/265 (47%), Gaps = 25/265 (9%) Query: 60 VSARVMDASPALRVISKHGTGIDTIDSQAAQQRGIVVKAAAGANAPAVAEHTWALIMACA 119 +S ++++ P L++I++ G ID AA RGI + G + A AE TW LIMA Sbjct: 59 LSRQLLEKLPNLKLIAQTGKVSGHIDLPAAAARGITIVEGVG-DPTAPAELTWTLIMAAM 117 Query: 120 KNVVGLDRRMRDGHWDKSTHKSLE------LKGRTLGLVGLGAIGRRVAAVAVALEMPVL 173 + + ++DG W ++ LKGRTL + G G IG+ +AA A M V+ Sbjct: 118 RKLPQYVNNLKDGLWQIASMSPARNTLGTALKGRTLAIWGYGRIGQMIAAYGKAFGMDVM 177 Query: 174 AY-----------DPYAKEAPQGVQLVDLDTLLAGSDVVSLHCPLTAENKHMINAQSLAR 222 + D Y + Q + +DV++LH L + ++ A LAR Sbjct: 178 VWGRPASCEAAVRDGYRAASSQ-------QAFFSEADVLTLHLRLNDATRGIVTADDLAR 230 Query: 223 MKDGAILVNTARGGLIDEQALIAALGSGKLLAAGLDSFEKEPFAAPHPLQQVGNAILSPH 282 MK A+ VNT+R L+ AL AAL G+ A LD FE EP PL ++ N + SPH Sbjct: 231 MKPQALFVNTSRAELVATDALQAALEQGRPGYAALDVFENEPLPPGSPLLRMENVLASPH 290 Query: 283 IGGVTTDAYIAMGTGAASNVLAVLN 307 +G V D+Y A N++ N Sbjct: 291 LGYVEKDSYELYFGSAFQNIVDFAN 315 >gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthinobacterium sp. Marseille] Length = 400 Score = 118 bits (295), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 73/227 (32%), Positives = 118/227 (51%), Gaps = 7/227 (3%) Query: 71 LRVISKHGTGIDTIDSQAAQQRGIVVKAAAGANAPAVAEHTWALIMACAKNVVGLDRRMR 130 L++I G G D D +AA + I V N+ +VAEH +I++ +N + ++ Sbjct: 115 LKMIVTAGIGSDHTDLEAANKHNITVAEVTYCNSHSVAEHVVMMILSQVRNYIPSYNQVI 174 Query: 131 DGHWD--KSTHKSLELKGRTLGLVGLGAIGRRVAAVAVALEMPVLAYD----PYAKEAPQ 184 +G W+ +S +L+ ++G V G IG RV + ++ + D P A E Sbjct: 175 NGGWNIADCVERSYDLEAMSVGTVAAGRIGLRVLRLLKPFDVKLHYMDRHRLPEAVEKEL 234 Query: 185 GVQL-VDLDTLLAGSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDEQAL 243 + LD+L DVV+L+CPL E +HMIN ++L K G+ ++NTARG L D A+ Sbjct: 235 NLTYHSTLDSLTKVCDVVTLNCPLHPETEHMINEKTLKNFKRGSYIINTARGKLCDRDAI 294 Query: 244 IAALGSGKLLAAGLDSFEKEPFAAPHPLQQVGNAILSPHIGGVTTDA 290 +AAL SG+L D + +P HP + + + ++PHI G + A Sbjct: 295 VAALKSGQLAGYAGDVWFPQPAPKDHPWRTMPHHGMTPHISGTSLTA 341 >gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthinobacterium sp. Marseille] Length = 336 Score = 99.8 bits (247), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 74/258 (28%), Positives = 122/258 (47%), Gaps = 16/258 (6%) Query: 67 ASPALRVISKHGTGIDTIDSQAAQQRGIVVKAAAGANAPAVAEHTWALIMACAKNVVGLD 126 A P L+++ G D D A +RG+ AE T L++ ++V+ D Sbjct: 64 ACPKLKIVFAALKGYDNFDVDACTKRGVWFGIVPDLLTVPTAELTVGLLLGLTRHVMAGD 123 Query: 127 RRMRDG--HWDKSTHKSLELKGRTLGLVGLGAIGRRVAAVAVALEMPVLAYD-----PYA 179 +R G H + L G T+G++G+G +G+ +A EM + D P Sbjct: 124 DHVRSGTFHGWRPKLYGAGLAGSTIGIIGMGRVGKAIAKRLSGFEMNAVYCDSVPLNPVD 183 Query: 180 KEAPQGVQLVDLDTLLAGSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLID 239 ++A Q V D LL SD V P+T++ H+I+A ++++M+ G+ L+NT+RG ++D Sbjct: 184 EQAWNARQ-VSFDELLTCSDFVVPMLPMTSDTFHLIDAHAISKMRRGSYLLNTSRGSVVD 242 Query: 240 EQALIAALGSGKLLAAGLDSFEKEPFAAPHPLQQVGNAIL--------SPHIGGVTTDAY 291 E A++ AL G L D FE E +A P V A+L +PH+G Sbjct: 243 ENAVVEALNQGHLAGYAADVFEMEEWARPDRPLTVPQALLNNRTQTLFTPHVGSGVKKVR 302 Query: 292 IAMGTGAASNVLAVLNTQ 309 + + +A ++L L Q Sbjct: 303 LEIERYSAHSILQALAGQ 320 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.317 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 610,976 Number of extensions: 20714 Number of successful extensions: 89 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 82 Number of HSP's successfully gapped: 4 Length of query: 313 Length of database: 1,221,021 Length adjustment: 83 Effective length of query: 230 Effective length of database: 914,170 Effective search space: 210259100 Effective search space used: 210259100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)