BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4337.p (404 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981853|ref|YP_001353483.1| CzcB family cobalt/zinc/cadmium... 438 e-124 gi|152983106|ref|YP_001353461.1| cobalt-zinc-cadmium resistance ... 382 e-107 gi|152981912|ref|YP_001352100.1| cation efflux protein [Janthino... 107 2e-24 gi|152983091|ref|YP_001353421.1| cobalt-zinc-cadmium resistance ... 92 4e-20 gi|152981890|ref|YP_001351977.1| cation efflux system protein [J... 82 5e-17 >gi|152981853|ref|YP_001353483.1| CzcB family cobalt/zinc/cadmium efflux transporter membrane fusion protein [Janthinobacterium sp. Marseille] Length = 422 Score = 438 bits (1127), Expect = e-124, Method: Compositional matrix adjust. Identities = 231/385 (60%), Positives = 289/385 (75%), Gaps = 7/385 (1%) Query: 27 GNGSQGENAKPSA--HQEEGGHQDNEHPGED-QDSDH-KE--EGKIALDQEQMAAAAIEL 80 G+G K +A H ++ H D EH E+ + +D KE E KI L Q+ AA + L Sbjct: 38 GDGEHHAENKSAAKDHSDDHKHADGEHHAEEGKKADAGKEAAENKILLSDAQIKAAGVSL 97 Query: 81 KTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVESVSATLGQKVKKGQVLAVINSSA 140 + A A I ++L LPGEI+FNEDRTAHVVP++AG+V+SV A LGQ+VKKGQVLAVI S+ Sbjct: 98 QVADAAHIKTALVLPGEIQFNEDRTAHVVPRLAGVVQSVPANLGQQVKKGQVLAVIASTG 157 Query: 141 ISELRSELLNAEQRLSLARTAYQREKKLWEQKISAEQDYLQAQSALREAEVAVRNAQQKL 200 +SE RSEL A++RL+LA+ Y+REKKLW++KISAEQDYLQAQ L E E+AV+NA+QKL Sbjct: 158 LSEQRSELFAAQKRLTLAKLTYEREKKLWQEKISAEQDYLQAQQVLHETEIAVQNARQKL 217 Query: 201 RAIGASGSSGS-LNRYEIRAPFDGTVVEKHLGLGEAVREDANAFLISDLSSVWAEIIVSP 259 A+GA + NRYEIRAPFDG VVEKH+ LGEAV+EDAN F +SDLS+VWA+I V Sbjct: 218 SALGADAGAAGAFNRYEIRAPFDGIVVEKHISLGEAVKEDANIFTLSDLSTVWADIAVPA 277 Query: 260 KDMQVVRVGEKVVVQATALSASAQGNISYVGSLIGEQSRTAKAHVVLPNTGEIWRPGLFV 319 KD+ VRVGEKV V+A+A ++A G ++YVGSL+G Q+RTA A V L N WRPGLFV Sbjct: 278 KDLNAVRVGEKVTVKASAFDSTATGTVAYVGSLLGVQTRTANARVTLANPQGTWRPGLFV 337 Query: 320 NVEVVSDEAQVPVAVAADAIQTVDGKNVVFVRVPGGFAAQEVTLGRASGKFVEIKSGIDA 379 NVE+VS+E +VPVAVAADAIQT++ K V+FV+V GF AQEV+ GR GK VEI G+ A Sbjct: 338 NVEIVSNEVEVPVAVAADAIQTINDKPVIFVKVADGFIAQEVSTGRTDGKRVEIVKGLKA 397 Query: 380 GTEYAATGSFAIKAELGKGSAEHSH 404 G+EYAA GSF IK+ELGK SAEH+H Sbjct: 398 GSEYAAGGSFVIKSELGKASAEHTH 422 >gi|152983106|ref|YP_001353461.1| cobalt-zinc-cadmium resistance protein CzcB [Janthinobacterium sp. Marseille] Length = 526 Score = 382 bits (981), Expect = e-107, Method: Compositional matrix adjust. Identities = 186/344 (54%), Positives = 250/344 (72%) Query: 61 KEEGKIALDQEQMAAAAIELKTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVESVS 120 +EEG++A+ EQ+ +++ AG ++I + LQL GEIR NEDRT VVP++AGIVESV Sbjct: 183 QEEGQVAMSDEQLTKNGVKILKAGPVRIKTELQLIGEIRLNEDRTVQVVPRLAGIVESVR 242 Query: 121 ATLGQKVKKGQVLAVINSSAISELRSELLNAEQRLSLARTAYQREKKLWEQKISAEQDYL 180 A G +V+KGQ+LAVI+S A+S+ RSELL A++RL+LART Y+REK LWE+KISAEQDYL Sbjct: 243 ANAGDRVRKGQILAVISSQALSDQRSELLAAQKRLALARTTYEREKTLWEEKISAEQDYL 302 Query: 181 QAQSALREAEVAVRNAQQKLRAIGASGSSGSLNRYEIRAPFDGTVVEKHLGLGEAVREDA 240 QA++ ++EA++ ++AQQKL A+GAS SS +L +YEIR+P DG + EK + G +EDA Sbjct: 303 QARTTMQEAQIIAQSAQQKLAALGASPSSTNLTQYEIRSPIDGVITEKRISAGTVAKEDA 362 Query: 241 NAFLISDLSSVWAEIIVSPKDMQVVRVGEKVVVQATALSASAQGNISYVGSLIGEQSRTA 300 N F ISDLS+VW ++ V +D+ V+ G+K V+ATA A G +SY+GSL+GEQ+R A Sbjct: 363 NIFDISDLSTVWVDLTVRAQDINAVKTGQKATVKATAFDAEGTGTVSYIGSLVGEQTRAA 422 Query: 301 KAHVVLPNTGEIWRPGLFVNVEVVSDEAQVPVAVAADAIQTVDGKNVVFVRVPGGFAAQE 360 KA +V+ N WRPGL VNV +VSDE VPVAV D+IQT+ VVF R F A+ Sbjct: 423 KARIVMANPKGTWRPGLAVNVALVSDEVDVPVAVTTDSIQTLGNATVVFGRYGELFEARP 482 Query: 361 VTLGRASGKFVEIKSGIDAGTEYAATGSFAIKAELGKGSAEHSH 404 + LGR K+VE+ G++ G +YA T SF IKAELGK SA H+H Sbjct: 483 LELGRRGAKYVEVLKGLETGEDYAGTNSFLIKAELGKASASHAH 526 >gi|152981912|ref|YP_001352100.1| cation efflux protein [Janthinobacterium sp. Marseille] Length = 383 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 84/322 (26%), Positives = 156/322 (48%), Gaps = 11/322 (3%) Query: 78 IELKTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVESVSATLGQKVKKGQVLAVIN 137 +++ T G + L++PG I F+ R A + V G + +SA LGQ VK G VLA ++ Sbjct: 51 VKIGTVGVAPFSDVLRVPGLIDFDRQRMARIGAPVTGRITDISANLGQSVKPGDVLARLH 110 Query: 138 SSAISELRSELLNAEQRLSLARTAYQREKKLWEQKISAEQDYLQAQSALREAEVAVRNAQ 197 S+ + + L A + L + R ++L+ + + + + ++ A R A Sbjct: 111 STELGSAQLAYLKARAQAELQQNNQTRARQLFAADVIGKAELQRRENESAVAAAESRAAA 170 Query: 198 QKLRAIGASGS-------SGSLNRY-EIRAPFDGTVVEKHLGLGEAVREDANAFLISDLS 249 ++L+ +G S + SG ++ Y + + G+VVE + G+ V+ F ++DLS Sbjct: 171 EQLKVLGMSSAAIARMAQSGEISSYAPVASTLHGSVVELKVSPGQVVQPADALFTVADLS 230 Query: 250 SVWAEIIVSPKDMQVVRVGEKVVVQATAL-SASAQGNISYVGSLIGEQSRTAKAHVVLPN 308 VWA V + +V VG+ V ++ AL + G + YVG ++ +RT + N Sbjct: 231 HVWAVAEVPEQQADMVAVGQTVDIEVPALGNLKLSGKLIYVGQIVDPATRTVHVRTAIDN 290 Query: 309 TGEIWRPGLFVNVEVVSDEAQVPVAVAADAIQTVDGKNVVFVRV-PGGFAAQEVTLGRAS 367 +P + + + S + + + A A+ + ++V+V PG F A +V LG+++ Sbjct: 291 KDGRLKPAMLATMLIASRPTE-QLVIPAAAVVRFENVDLVYVETQPGHFRATKVKLGQSN 349 Query: 368 GKFVEIKSGIDAGTEYAATGSF 389 + + SG+ AG A G+F Sbjct: 350 NGTLVVASGLKAGDRIAVDGAF 371 >gi|152983091|ref|YP_001353421.1| cobalt-zinc-cadmium resistance protein CzcB [Janthinobacterium sp. Marseille] Length = 396 Score = 92.4 bits (228), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 98/355 (27%), Positives = 166/355 (46%), Gaps = 26/355 (7%) Query: 58 SDHKEEGKIALDQEQMAAAAIELKTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVE 117 SD +E KI E ++ + I ++ G+ + + + G + + A ++ + +G V Sbjct: 58 SDSGDEVKI--PAEYLSVSKITVEAIGSGGLEAEILTAGTVVAAPNSEAVIMARASGNVT 115 Query: 118 SVSATLGQKVKKGQVLAVINSSAISELRSELLNAEQ-----RLSLARTAYQREKKLWEQK 172 VS LG V+ G+ LA +S L + +NA++ R+ LAR Y RE L+EQ Sbjct: 116 QVSRQLGDTVRAGETLA-----QVSSLEAAAMNADRNVAIARVDLARKNYAREYSLFEQG 170 Query: 173 ISAEQDYLQAQSALREAEVAVRNAQQKLRA--IGASGSSGSLNRYEIRAPFDGTVVEKHL 230 ++ Q+ AQSAL AE A +A + A G S + +P G + + + Sbjct: 171 VTPRQEMEAAQSALTVAESEALRATSVAKAAQVTADGKS-----VAVVSPISGKITAQTV 225 Query: 231 GLGEAVREDANAFLISDLSSVWAEIIVSPKDMQVVRVGEKVVVQATALSASAQGNISYVG 290 LG V+ F ++ V E + D+ V G+ + A A +S + + V Sbjct: 226 TLGIFVQPQMELFRVAGNGKVLIEASLPVADVTRVASGDNATILA-ANGSSVEATVRSVT 284 Query: 291 SLIGEQSRTAKAHVVLPNTGE---IWRPGLFVNVEVVSDEAQVPVAVAADAIQTVDGKNV 347 + +RTA V+ P++ I G+ + V + + + V+ DA+Q V+G +V Sbjct: 285 PTVSSSTRTAIV-VLTPSSSTRELIVGEGVQARLHVKNGASG--LVVSEDAVQNVNGHDV 341 Query: 348 VFVRVPGGFAAQEVTLGRASGKFVEIKSGIDAGTEYAATGSFAIKAELGKGSAEH 402 +FVR GF Q V +G SG +I SG+ AG A +F +KA++ K + E Sbjct: 342 LFVRTKEGFRVQPVLVGTRSGGVAQIISGVSAGEMVATRNAFLVKADMIKSAKEE 396 >gi|152981890|ref|YP_001351977.1| cation efflux system protein [Janthinobacterium sp. Marseille] Length = 389 Score = 82.0 bits (201), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 81/324 (25%), Positives = 143/324 (44%), Gaps = 5/324 (1%) Query: 76 AAIELKTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVESVSATLGQKVKKGQVLAV 135 A IE + A + QL + ++ED TA + ++G + ++ A G VK GQVLA Sbjct: 60 AMIEAQNIPASPVPLGDQLSAHLVYDEDVTARIGVSISGRIVALKAAPGDVVKAGQVLAE 119 Query: 136 INSSAISELRSELLNAEQRLSLARTAYQREKKLWEQKISAEQDYLQAQSALREAEVAVRN 195 I+S + +++ A+ L R Y+R K L E + A +D AQ+ +A+ Sbjct: 120 IDSPDVGTAYADINKAKADLDHKRRVYERAKDLGEGEAIAAKDVENAQADYVQAQAETAR 179 Query: 196 AQQKLRAIGASGSSGSLNRYEIRAPFDGTVVEKHLGLGEAVREDANA--FLISDLSSVWA 253 A+ +L+ + +G R + +P +G V E+ V ++ F++++ +W Sbjct: 180 AELRLKNLNPNGMVIRGQRISLLSPMNGVVTERTATPALEVSPGMSSPLFVVTNPKQLWL 239 Query: 254 EIIVSPKDMQVVRVGEKVVVQATAL-SASAQGNISYVGSLIGEQSRTAKAHVVLPNTGEI 312 I V K + +++G V +++ A + I +G + SR L N Sbjct: 240 MIDVPEKMLSQIKLGSTVAIESDAYPNEKFTAKIVQLGQAVDPNSRRITIRARLNNPDGK 299 Query: 313 WRPGLFVNVEVVSDEAQVPVAVAADAIQTVDGKNVVFVRV-PGGFAAQEVTLGRASGKFV 371 P +FV +V ++ VAV A+ T + VFV+ PG F ++V L Sbjct: 300 LLPEMFVRA-LVQQDSGTGVAVPNGALVTRGIYSFVFVQTAPGEFQQRKVKLLTRGSSVS 358 Query: 372 EIKSGIDAGTEYAATGSFAIKAEL 395 + G+ G G+ + AEL Sbjct: 359 YVGEGLKGGESIVTKGALLLDAEL 382 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.311 0.127 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 865,311 Number of extensions: 34563 Number of successful extensions: 150 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 13 Length of query: 404 Length of database: 1,221,021 Length adjustment: 85 Effective length of query: 319 Effective length of database: 906,776 Effective search space: 289261544 Effective search space used: 289261544 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 130 (54.7 bits)