BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4262.p (616 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982025|ref|YP_001353626.1| glutathione-regulated potassium... 342 5e-95 gi|152982009|ref|YP_001355036.1| glutathione-regulated potassium... 253 3e-68 gi|152982911|ref|YP_001352645.1| glutathione-regulated potassium... 182 5e-47 gi|152980839|ref|YP_001353999.1| antiporter-2, CPA2 family monov... 105 9e-24 >gi|152982025|ref|YP_001353626.1| glutathione-regulated potassium-efflux system protein kefB [Janthinobacterium sp. Marseille] Length = 594 Score = 342 bits (876), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 226/568 (39%), Positives = 337/568 (59%), Gaps = 33/568 (5%) Query: 6 LIALVYLL-AAVAAVPLAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVL 64 L+ +V LL AAV VPL KR+GLG+VLGYL AG+ IGP GLGL+++ + I+ +E GVV+ Sbjct: 10 LVGIVTLLGAAVVGVPLFKRIGLGSVLGYLAAGLAIGPSGLGLMSDAQTIIHVAELGVVM 69 Query: 65 LLFLIGLELDPKRLWALRRSIFGWGSVQVGAVSLALFAAALAAGVPWRIGLIAALGMSLS 124 LF+IGLE+ P LW+LR IFG GS+QV A S+ L + G PW++ +A G L+ Sbjct: 70 FLFIIGLEMKPSHLWSLRGQIFGLGSMQVVASSILLTGVGIVFGFPWQVSFVAGAGFVLT 129 Query: 125 STAIALATLGERKLTATPAGATGFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGWLHAG 184 STAI + L ER TP G SILLF+D+ +P++A V L A + + H Sbjct: 130 STAIVMQVLSERGDIQTPRGQRIVSILLFEDLLIVPLLAIVAFLAPADAAAT-----HGA 184 Query: 185 QV-------ALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVISTCLLMQSVGM 237 V AL + +LI G L+ P+ R++AK RE+ TA ALL+V+ LLM+ G+ Sbjct: 185 PVLQKIIFAALSLTVLIGAGIWLLNPLFRVLAKAKAREVMTAAALLVVLGAALLMELGGL 244 Query: 238 SMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWR-IIG 296 SMA+G FLAGVLL++S +RH LE+D+EPF+GLLLGLFF+ VGM+++ V +A W+ +I Sbjct: 245 SMAMGAFLAGVLLSESTFRHQLEADIEPFRGLLLGLFFLGVGMALNLDV-VASNWQLVIA 303 Query: 297 LVIGFLAIKTAVMWFLGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISS 356 V+ + K ++ + R + + A L++QGGEFAF++F A+ V++ EI++ Sbjct: 304 GVLALMLAKAFCIYCVARLAKSNHAEAIDRAILMAQGGEFAFVLFTEALKLNVISAEINA 363 Query: 357 LLVVVVALSMLVTPLLLLVHDRLLVRWLEGGK--------RRPEDRIGPQDNAVIIAGFG 408 + +V LSM +TP+++++H R GGK P D +V+I GFG Sbjct: 364 NITAIVVLSMALTPMVVVLHKRF------GGKPGVSLDGIEEPRD----VQASVLIIGFG 413 Query: 409 RFGQIIGRLLAANDISATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLV 468 R GQI + A +++D+DPD I + +GFKVFYGD R D+L AGA +A V++ Sbjct: 414 RVGQIASQGPLAKGAKLSIIDNDPDVIRAVEPYGFKVFYGDGGRPDILHAAGALQASVIL 473 Query: 469 IAIDDAQAALEMVDKVREEFPHLQIAARARNVTHYYDLMDRGVTILERETFESALHMGRQ 528 + I+D A +V+ ++E PH ++ RA + H +L+ + RETFE++L M +Q Sbjct: 474 VCINDKNAVTRIVETAKQECPHTKLVVRAFDREHALELVKHDADFVVRETFEASLQMAKQ 533 Query: 529 VLQQLDFGAYRAREAAMKFRAHNLKTLA 556 + L A E R+ + + A Sbjct: 534 AVILLGSEVQEAEELIADVRSRDAERFA 561 >gi|152982009|ref|YP_001355036.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Janthinobacterium sp. Marseille] Length = 682 Score = 253 bits (646), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 181/559 (32%), Positives = 288/559 (51%), Gaps = 19/559 (3%) Query: 23 KRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVLLLFLIGLELDPKRLWALR 82 + + L +LGYL+ GI+IGP+ LG + EFGVV L+F IGLE +L A+R Sbjct: 43 RMMHLPPMLGYLVVGILIGPYSLGWAEDNATTHALGEFGVVFLMFSIGLEFSLSKLLAMR 102 Query: 83 RSIFGWGSVQV----GAVSL-----ALFAAALAAGVPWRIGLIAALGMSLSSTAIALATL 133 R++FG G QV GA + A F+A L+A + W+ L +++SSTAI L Sbjct: 103 RTVFGLGMAQVLLTIGATMVFGWLVAHFSARLSA-ISWQAALAIGGALAMSSTAIVSKLL 161 Query: 134 GERKLTATPAGATGFSILLFQDIAAIPMMAAVPLLGVAASQ-GSGQGWLHAGQVALVIAL 192 ER + G ILLFQD+A +P++ +P L G+ W A + +V+ L Sbjct: 162 TERLELESQHGRRIIGILLFQDLAVVPLLILIPALANPRGDLGTTLLW-AALKAVIVLFL 220 Query: 193 LIFGGRVLMRPILRIIAKTDMREIFTAFALLLVISTCLLMQSVGMSMALGTFLAGVLLAD 252 L+F G L+R RI+ K +E+F LL+ + + + G+S+ALG F+AG+L+++ Sbjct: 221 LLFIGHKLIRSWFRIVVKRRSQELFMLNLLLITLGAAWITEMAGLSLALGAFVAGMLISE 280 Query: 253 SEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRIIGLVIGFLAIKTAVMWFL 312 +EY+H +E D++ F+ +LLGLFFI VGM ++ + + W ++ L+ G + +K ++ FL Sbjct: 281 TEYKHQVEEDIKSFRDVLLGLFFITVGMLLNVRLVVEHWWLVLLLLSGVVLLKFGLIAFL 340 Query: 313 GRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSLLVVVVALSMLVTPLL 372 RCFG P G L L+Q GEF F++ ++ P I L++ + LSML PL+ Sbjct: 341 ARCFGAPVGVALRTGLALAQAGEFGFVLLNQVGGLNLVEPLILQLILASMVLSMLAAPLI 400 Query: 373 LLVHDRLLVR-----WLEGGKRRPE--DRIGPQDNAVIIAGFGRFGQIIGRLLAANDISA 425 + D ++++ W+ + R VIIAGFGR GQ + +LL A I+ Sbjct: 401 IAKSDAIVMKLSANEWMVQSLALTQIATRTMATQQHVIIAGFGRSGQSLAKLLEAEGITY 460 Query: 426 TVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEMVDKVR 485 LD DPD++ + G V YGDA R + L G ++A LVI +A++++ + Sbjct: 461 QALDMDPDRVSEAQAAGASVSYGDAGRRESLVAVGINRAAALVITYASTPSAMKVLHHMH 520 Query: 486 EEFPHLQIAARARNVTHYYDLMDRGVTILERETFESALHMGRQVLQQLDFGAYRAREAAM 545 + P+L + R+ + T L G + + E E +L + + L R Sbjct: 521 DLAPNLPVIVRSHDDTDLDKLRAAGASEVVPEAMEGSLMLASHAMLTLGVPLRRVVHRVQ 580 Query: 546 KFRAHNLKTLAALYPHYKD 564 R+ TL + D Sbjct: 581 AARSERYATLRGFFHGSSD 599 >gi|152982911|ref|YP_001352645.1| glutathione-regulated potassium-efflux system protein kefB [Janthinobacterium sp. Marseille] Length = 575 Score = 182 bits (462), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 152/456 (33%), Positives = 233/456 (51%), Gaps = 19/456 (4%) Query: 5 LLIALVYLLAAVAAVPLAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVL 64 L+ A+V+L + +++ +GL ++GY++ G+ + G GL+ I +E GVV Sbjct: 15 LVPAIVFLGLGITTAIVSRAVGLSPIVGYIVLGLGLNISGFGLLYGKSTISLLAELGVVF 74 Query: 65 LLFLIGLELDPKRLWALRRSIFGWGSVQVGAVSLALFAAALAAGVPWRIGLIAALGMSLS 124 LLF IGL R+ IF +G QV + L AL G+ L+ ++LS Sbjct: 75 LLFDIGLHFSLDRIRKQASDIFAFGPFQVIFATTGLTLTALVLGISIVPALLIGSILALS 134 Query: 125 STAIALATLGERKLTATPAGATGFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGWLHAG 184 STA+ + ER P G T SIL+FQD+AAI LL VA S G Sbjct: 135 STAVVGRLIAERHQQNCPVGLTATSILIFQDVAAIF------LLIVANSLDDGGTIWTVA 188 Query: 185 QVALVIALLIFG-----GRVLMRPILRIIAKTDMREIFTAFALLLVISTCLLMQSVGMSM 239 +AL A+L F R+L+ +L ++A++ E+FTA ALL+ ++ +G+S+ Sbjct: 189 ALALCKAILAFAITVAISRLLIGSLLGLVARSKNEEVFTAAALLIALAAGWATGKMGLSL 248 Query: 240 ALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRIIGLVI 299 LG FL G+ LA++ YR +ES++ PF+GLLLG FFI++G+S++ V IIGL I Sbjct: 249 TLGAFLGGLALAETPYRAVIESEIRPFRGLLLGFFFISIGLSLNVNVLAHSLPLIIGLTI 308 Query: 300 GFLAIKTAVMWFLGRCF--GIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSL 357 +K R F +P QL FLL+QG EFAF++ + +S+ Sbjct: 309 LLFIVKIISNITASRVFKWSVPGSTQL--GFLLAQGSEFAFVILSLPSMQSAIGDTNTSV 366 Query: 358 LVVVVALSMLVTPLLLLVHDRLL--VRWLEGGKRRPEDRIGPQ-DNA-VIIAGFGRFGQI 413 ++ VVALSM VTP L V +L +R + K + + P+ DNA +II G G G+ Sbjct: 367 IIAVVALSMAVTPNLADVGRKLAGRMRMRKRVKEALDAELTPKIDNAPIIIVGMGTIGRT 426 Query: 414 IGRLLAANDISATVLDHDPDQIDFLRKFGFKVFYGD 449 I L +I ++ D +++ G+ +GD Sbjct: 427 IADALIKFEIGYYAVEFDQQRLEMAIADGYDATFGD 462 >gi|152980839|ref|YP_001353999.1| antiporter-2, CPA2 family monovalent cation/proton antiporter [Janthinobacterium sp. Marseille] Length = 584 Score = 105 bits (261), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 103/365 (28%), Positives = 173/365 (47%), Gaps = 40/365 (10%) Query: 30 VLGYLLAGIVIGPWG--LGLITEVKHILEFSEFGVVLLLFLIGLELDPKRLWALRRSIFG 87 VLGY+LAG++IGP LI + + I +E GVV L+F +GLE + K+L Sbjct: 32 VLGYILAGVIIGPHTPPFELIQDDRTIKIVAELGVVFLMFSLGLEFNLKKL--------- 82 Query: 88 WGSVQVGAVSLALFAAALAAGVPWRIG----------LIAALGMSLSSTAIALATLGERK 137 V V A A L + + IG L +++SST I + L E Sbjct: 83 -SRVGVTAFIAAFGEIVLMTWIGYEIGRYFEWNTMDSLFLGAMLAISSTTIIVKALDELG 141 Query: 138 LTATPAGATGFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGWLHAGQVALVIALLIFGG 197 L F IL+ +DI AI M+A + + + S +G ++ G+++L + + + G Sbjct: 142 LKHERFAHLIFGILIVEDILAIGMIALLSGIAMNGSVDAGDVFVTVGKLSLFMIVALVLG 201 Query: 198 RVLMRPILRIIAKTDMREIFTAFALLLVISTCLLMQSVGMSMALGTFLAGVLLADSEYRH 257 +++ +L +AK E+F L L CLL+ + S+ALG F+ G ++A++ + Sbjct: 202 LLMVPRLLAYVAKFKSNEVFLITVLALCFGFCLLVLQMEYSIALGAFMIGAIMAEARQLN 261 Query: 258 ALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRIIGLVIGFLAIKTAVMWFLGRCFG 317 +E + P + + LFF+ VG+ L P ++ + I AV+ LG+ F Sbjct: 262 LIERLIAPIRDMFSALFFVGVGL-------LFNPTVLVTYALPIALITVAVV--LGKVFA 312 Query: 318 IPRG---------QQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSLLVVVVALSMLV 368 G + LSQ GEF+FI+ +T +V + + ++V V A++ L+ Sbjct: 313 CGLGTFVAGNDGRTSMRVGMGLSQIGEFSFIIASLGLTLQVTSEFLYPIVVAVSAVTTLL 372 Query: 369 TPLLL 373 TP L+ Sbjct: 373 TPYLI 377 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.327 0.142 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,158,895 Number of extensions: 46139 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 4 Length of query: 616 Length of database: 1,221,021 Length adjustment: 89 Effective length of query: 527 Effective length of database: 891,988 Effective search space: 470077676 Effective search space used: 470077676 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)