BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4168.p (328 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid... 489 e-140 gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Ja... 135 3e-33 gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthi... 66 3e-12 gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthino... 62 4e-11 >gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 323 Score = 489 bits (1260), Expect = e-140, Method: Compositional matrix adjust. Identities = 232/322 (72%), Positives = 273/322 (84%) Query: 1 MKIAILDDYQDAVRHLDCFSLLAGHDVKVFNNTVRGIGQLTARLSPFDAVVLIRERTALT 60 MKIAILDDYQD+VRHLDCFSLLAGH+VK+F N RG+GQL RL+ FDA+VLIRERT L+ Sbjct: 1 MKIAILDDYQDSVRHLDCFSLLAGHEVKIFTNGARGVGQLAIRLASFDALVLIRERTVLS 60 Query: 61 RPLLEKLPLLKLISQTGKVSGHVDVAAATERGIAIVEGVGDPTAPAELTWALIMAAMRRV 120 R LLEKLP LKLI+QTGKVSGH+D+ AA RGI IVEGVGDPTAPAELTW LIMAAMR++ Sbjct: 61 RQLLEKLPNLKLIAQTGKVSGHIDLPAAAARGITIVEGVGDPTAPAELTWTLIMAAMRKL 120 Query: 121 PQYCSALRAGAWQQVSPTPHHNMIGTALKGRTLGIWGYGKIGKLVAGYGRAFGMSVLIWG 180 PQY + L+ G WQ S +P N +GTALKGRTL IWGYG+IG+++A YG+AFGM V++WG Sbjct: 121 PQYVNNLKDGLWQIASMSPARNTLGTALKGRTLAIWGYGRIGQMIAAYGKAFGMDVMVWG 180 Query: 181 REASREQAVKDGYQAAASKEEFFAQADVLSLHLRLNDATRAIVKAADLARMKPTACLVNT 240 R AS E AV+DGY+AA+S++ FF++ADVL+LHLRLNDATR IV A DLARMKP A VNT Sbjct: 181 RPASCEAAVRDGYRAASSQQAFFSEADVLTLHLRLNDATRGIVTADDLARMKPQALFVNT 240 Query: 241 SRAELVEPEALEAALQAGRPGLAALDVYEREPLPVESPLLQMDNVVAAPHLGYVEKDGYE 300 SRAELV +AL+AAL+ GRPG AALDV+E EPLP SPLL+M+NV+A+PHLGYVEKD YE Sbjct: 241 SRAELVATDALQAALEQGRPGYAALDVFENEPLPPGSPLLRMENVLASPHLGYVEKDSYE 300 Query: 301 LYFRAAFQNIVDYAAGRPANVL 322 LYF +AFQNIVD+A G P NVL Sbjct: 301 LYFGSAFQNIVDFANGTPKNVL 322 >gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 327 Score = 135 bits (341), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 107/312 (34%), Positives = 154/312 (49%), Gaps = 15/312 (4%) Query: 19 FSLLAGHDVKVFNNTVRGIGQLTARLSPFDAVVLIRERTALTRPLLEKLPLLKLISQTGK 78 F + + + K+F+ +L ++ DAVV+ ++ ++ P LK I Sbjct: 23 FEVESNQEDKIFSP-----AELLQKMQGKDAVVVTASEK-MSADVIAANPQLKAICNVAV 76 Query: 79 VSGHVDVAAATERGIAIVE--GVGDPTAPAELTWALIMAAMRRVPQYCSALRAGAWQQVS 136 ++DVAAAT G+ + V + T A+ W L+MA RRV + LRAG W+ Sbjct: 77 GYNNIDVAAATAAGVMVTNTPDVLNETT-ADYAWTLLMANARRVCESEHWLRAGNWKHWR 135 Query: 137 PTPHHNMIGTALKGRTLGIWGYGKIGKLVAGYGRAFGMSVLIWGRE-ASREQAVKDGYQA 195 + +G + G TLGI G G+IG+ +A F M+V+ R A+ EQ Sbjct: 136 ---FDSFLGADIHGATLGILGMGRIGQAIARRSTGFDMNVIYHNRSRATPEQEAYANNAR 192 Query: 196 AASKEEFFAQADVLSLHLRLNDATRAIVKAADLARMKPTACLVNTSRAELVEPEALEAAL 255 SKEE AD L L L + T I+ AA+LA MKPTA LVN +R +V+ AL AAL Sbjct: 193 YVSKEELLRSADHLILVLPYSPQTHHIIGAAELALMKPTATLVNIARGGIVDDVALIAAL 252 Query: 256 QAGRPGLAALDVYEREPLPVESPLLQMDNVVAAPHLGYVEKDGYELYFRAAFQNIVDYAA 315 + R A LDV+E EP + L + NVV PH+G + A N+ + Sbjct: 253 RENRIASAGLDVFENEP-ALHPDFLTLSNVVLTPHIGSGSEKTRRAMADCASANLAAAMS 311 Query: 316 G-RPANVLNPEV 326 G +P N+LNPEV Sbjct: 312 GQQPPNLLNPEV 323 >gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthinobacterium sp. Marseille] Length = 336 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 76/274 (27%), Positives = 121/274 (44%), Gaps = 20/274 (7%) Query: 63 LLEKLPLLKLISQTGKVSGHVDVAAATERGI---AIVEGVGDPTAPAELTWALIMAAMRR 119 L P LK++ K + DV A T+RG+ + + + PTA ELT L++ R Sbjct: 61 FLAACPKLKIVFAALKGYDNFDVDACTKRGVWFGIVPDLLTVPTA--ELTVGLLLGLTRH 118 Query: 120 VPQYCSALRAGAWQQVSPTPHHNMIGTALKGRTLGIWGYGKIGKLVAGYGRAFGMSVLIW 179 V +R+G + P + G L G T+GI G G++GK +A F M+ ++ Sbjct: 119 VMAGDDHVRSGTFHGWRP----KLYGAGLAGSTIGIIGMGRVGKAIAKRLSGFEMNA-VY 173 Query: 180 GREASREQAVKDGYQA-AASKEEFFAQADVLSLHLRLNDATRAIVKAADLARMKPTACLV 238 + + A S +E +D + L + T ++ A +++M+ + L+ Sbjct: 174 CDSVPLNPVDEQAWNARQVSFDELLTCSDFVVPMLPMTSDTFHLIDAHAISKMRRGSYLL 233 Query: 239 NTSRAELVEPEALEAALQAGRPGLAALDVYERE-------PLPVESPLL-QMDNVVAAPH 290 NTSR +V+ A+ AL G A DV+E E PL V LL + PH Sbjct: 234 NTSRGSVVDENAVVEALNQGHLAGYAADVFEMEEWARPDRPLTVPQALLNNRTQTLFTPH 293 Query: 291 LGY-VEKDGYELYFRAAFQNIVDYAAGRPANVLN 323 +G V+K E+ +A + A RP LN Sbjct: 294 VGSGVKKVRLEIERYSAHSILQALAGQRPDGALN 327 >gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthinobacterium sp. Marseille] Length = 400 Score = 62.0 bits (149), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 65/276 (23%), Positives = 113/276 (40%), Gaps = 15/276 (5%) Query: 46 PFDAVVLIRERTALTRPLLEKLPLLKLISQTGKVSGHVDVAAATERGIAIVE-GVGDPTA 104 PF + ER A K LK+I G S H D+ AA + I + E + + Sbjct: 98 PFWPAYMTAERIA-------KAKKLKMIVTAGIGSDHTDLEAANKHNITVAEVTYCNSHS 150 Query: 105 PAELTWALIMAAMRR-VPQYCSALRAGAWQQVSPTPHHNMIGTALKGRTLGIWGYGKIGK 163 AE +I++ +R +P Y + G W L+ ++G G+IG Sbjct: 151 VAEHVVMMILSQVRNYIPSYNQVINGG-WNIADCVER----SYDLEAMSVGTVAAGRIGL 205 Query: 164 LVAGYGRAFGMSVLIWGREASREQAVKD-GYQAAASKEEFFAQADVLSLHLRLNDATRAI 222 V + F + + R E K+ ++ + DV++L+ L+ T + Sbjct: 206 RVLRLLKPFDVKLHYMDRHRLPEAVEKELNLTYHSTLDSLTKVCDVVTLNCPLHPETEHM 265 Query: 223 VKAADLARMKPTACLVNTSRAELVEPEALEAALQAGRPGLAALDVYEREPLPVESPLLQM 282 + L K + ++NT+R +L + +A+ AAL++G+ A DV+ +P P + P M Sbjct: 266 INEKTLKNFKRGSYIINTARGKLCDRDAIVAALKSGQLAGYAGDVWFPQPAPKDHPWRTM 325 Query: 283 DNVVAAPHLGYVEKDGYELYFRAAFQNIVDYAAGRP 318 + PH+ Y + + Y GRP Sbjct: 326 PHHGMTPHISGTSLTAQTRYAAGTREILECYFEGRP 361 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.320 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 718,726 Number of extensions: 27669 Number of successful extensions: 96 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 4 Length of query: 328 Length of database: 1,221,021 Length adjustment: 84 Effective length of query: 244 Effective length of database: 910,473 Effective search space: 222155412 Effective search space used: 222155412 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)