BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4095.p (298 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980168|ref|YP_001352071.1| 2-hydroxy-3-oxopropionate reduc... 115 3e-27 gi|152980427|ref|YP_001352509.1| 3-hydroxyisobutyrate dehydrogen... 100 1e-22 gi|152980331|ref|YP_001351960.1| 2-hydroxy-3-oxopropionate reduc... 83 2e-17 >gi|152980168|ref|YP_001352071.1| 2-hydroxy-3-oxopropionate reductase [Janthinobacterium sp. Marseille] Length = 295 Score = 115 bits (288), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 78/284 (27%), Positives = 136/284 (47%), Gaps = 8/284 (2%) Query: 5 VGVIGLGAMGYGVASSLLRAGFNVHACDVR---KEVLDKFAAAGGVACANPAELAAKVDV 61 VG IGLG MG +A +L + G + D + +E++D G C + AE+A D+ Sbjct: 4 VGFIGLGIMGAPMALNLQKGGHQLFLHDQKAPAQELID----GGATVCKSGAEVAKSADI 59 Query: 62 VITLVVNAAQTEMVLFGENGAVRAMQPGSVVISSATVAPDFAVELGQRLAEKGLLLLDAP 121 +IT+V + + VLF ENG + G +V+ ++++P E +++ G LDAP Sbjct: 60 IITMVPDTPHVDAVLFAENGVAAGLSKGKIVVDMSSISPIATKEFAKKINALGCEYLDAP 119 Query: 122 VSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAG 181 VSGG A + +T+M G + ++ + M GK L +G G K+ NQ++ Sbjct: 120 VSGGEVGAKAASLTIMIGGSEAAFNTVKPLFELM-GKNITLVGGNGDGQTTKVANQIIVA 178 Query: 182 VHIAASAEAMALGLREGVNADALYDVITHSAGNSWMFENRVPHILNGDYTPLSAVDIFVK 241 ++I A +EA+ + G + + + NS + E ++ + P +++ K Sbjct: 179 LNIQAVSEALLFASKAGADPAKVRQALMGGFANSRILEVHGERMVKRTFNPGFRIELHQK 238 Query: 242 DLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIK 285 DL L L A+ LP +A ++ + G G D SA+ + Sbjct: 239 DLNLALQGAKAMGVSLPNTANTQELMNACAANGMSGLDHSALCR 282 >gi|152980427|ref|YP_001352509.1| 3-hydroxyisobutyrate dehydrogenase [Janthinobacterium sp. Marseille] Length = 298 Score = 100 bits (248), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 77/289 (26%), Positives = 131/289 (45%), Gaps = 7/289 (2%) Query: 3 RNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACA-NPAELAAKVDV 61 RN+G IG+G MG +A L R GF++ D + FA G A + AE+ D Sbjct: 5 RNIGFIGIGNMGAPMAGQLARKGFSLTVYDTHATTANAFAQEHGARVARSAAEVGIGADA 64 Query: 62 VITLVVNAAQTEMVLFGENGAVRAMQPGSVVISSATVAPDFAVELGQRLAEKGLLLLDAP 121 VI ++ + VLF +G + PGS+ I T AP + LA G+ LDAP Sbjct: 65 VIFMLPSDQVVRQVLF-TDGLATQLAPGSIAIDMGTSAPAVTRSISAELATLGIGYLDAP 123 Query: 122 VSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAG 181 V GG A + +M SG + + M K++ GD G +K + + Sbjct: 124 VMGGVVFAKDASLDIMVSGDDASIERCRPLFDAMGRKLWPCGDI-GSAHVLKAMTNYINA 182 Query: 182 VHIAASAEAMALGLREGVNADAL---YDVITHSAGNSWMFENRVPHILNGDYTPLSAVDI 238 + + EAM +G + G+++ + DV+ + + + + +PH+L + A+ + Sbjct: 183 CALINTLEAMTIGRKFGLDSKVMAEAIDVMCNGRQHP-VVKKIIPHVLTHKFGTGMAMQL 241 Query: 239 FVKDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIF 287 KD+ + +D+A +PL A +++ A G D S ++K + Sbjct: 242 IAKDVKIAVDSAHSVGAAVPLGEATEKLWRAACEQIGGSRDHSEIVKYW 290 >gi|152980331|ref|YP_001351960.1| 2-hydroxy-3-oxopropionate reductase [Janthinobacterium sp. Marseille] Length = 304 Score = 83.2 bits (204), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 85/284 (29%), Positives = 136/284 (47%), Gaps = 14/284 (4%) Query: 5 VGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAAKVDVVIT 64 + +G+G MG +AS LL+AG+ V A + + D+ A G A+ A D+VIT Sbjct: 10 IAFLGIGLMGKPMASRLLQAGYPVTAWNRTRSKADELAPLGASVAERVADAVAAADIVIT 69 Query: 65 LVVNAAQTEMVLFGENGAVRAMQPGSVVISSATVAPDFAVELGQRLAEKGLLLLDAPVSG 124 ++ V+ + A+ ++ G++VI ++ A E+ +LA +G+ +DAPVSG Sbjct: 70 MLEAGPIVAQVI---DAALPGLKHGALVIDMSSTRQSEAQEVHAKLAAQGVRFIDAPVSG 126 Query: 125 GAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHI 184 G A +G + +M G A+ +A+ E VL M G+ +G G G K+ NQL+ G + Sbjct: 127 GVVGAEAGSLAIMAGGSAQDFAEAEAVLTVM-GRPTLVGPA-GCGQIAKLCNQLIVGGTL 184 Query: 185 AASAEAMALGLREGVNADALYDVITHSAGNSWMFENRVPHILNGDYTPLSAVDIFVKDLG 244 AEA+ L G + A+ I S + E +L+ ++ P V KDL Sbjct: 185 NIVAEALLLAQAGGADPTAVRAAIRGGFAESRILEVHGQRMLDRNFMPGGQVKSQFKDLE 244 Query: 245 LVLDTARRSKFPLPLSAAA----HQMFMMASTAGHGGEDDSAVI 284 VL A + LP++ HQ+ A A D SAV+ Sbjct: 245 NVLIAANNAGLRLPVTELVTENYHQVLQNAPQA-----DQSAVL 283 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.318 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 676,986 Number of extensions: 26725 Number of successful extensions: 125 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 3 Length of query: 298 Length of database: 1,221,021 Length adjustment: 83 Effective length of query: 215 Effective length of database: 914,170 Effective search space: 196546550 Effective search space used: 196546550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)