BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3768.p (700 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982608|ref|YP_001352175.1| nitrate reductase catalytic sub... 158 1e-39 gi|152981034|ref|YP_001352093.1| formate dehydrogenase alpha sub... 136 4e-33 gi|152981248|ref|YP_001355342.1| NAD-dependent formate dehydroge... 115 9e-27 gi|152982393|ref|YP_001353660.1| formate dehydrogenase large sub... 105 7e-24 >gi|152982608|ref|YP_001352175.1| nitrate reductase catalytic subunit [Janthinobacterium sp. Marseille] Length = 939 Score = 158 bits (400), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 185/745 (24%), Positives = 305/745 (40%), Gaps = 82/745 (11%) Query: 4 TIVRAACPHDCPDTCALLVTVKDGVATEVRGDPDHPPTSGVLCTKVSRYAERTYH----- 58 T +A C + C C +L+ VRGDPDHP G LCTK S T H Sbjct: 22 TETKATCCY-CGVGCGVLIQSDGEKVVAVRGDPDHPANYGRLCTKGS-----TLHLTARP 75 Query: 59 ----QDRLLHPMRRVGKKGEGKFERISWEQAISTIAQRLGEIAR-RDPQAILPYSYAGTM 113 Q R L+P R+ + + ER SW + +A + R P ++ Y Sbjct: 76 ALQQQARALYPELRLARTDQR--ERASWATTLDFLANKFAATIRDHGPDSVGFY----LS 129 Query: 114 GQVQGESMAGRFFNRL-----GASQLERT--ICSSAGGAGYRYTIGTRVGSDL-EQVDHA 165 GQ+ E FN+L G + ++ +C S+ AGY+ T+G E +D A Sbjct: 130 GQLLTEDY--YVFNKLAKGLIGTNNVDTNSRLCMSSAVAGYKQTLGADAPPPCYEDIDQA 187 Query: 166 KLILIWGGNPIASNLHFWTRVQAAKRN--GARLIAIDPYRSLTAEKCHQHIALLPGTDAA 223 +I + G N ++ + R++ A++ ++I DP R+ TA + + +LPGTD A Sbjct: 188 DVIFLVGSNTAYAHPIVYRRIEDARKANPALKVIVADPRRTDTAREADLFLPILPGTDVA 247 Query: 224 LALGLMHVLIAEDLLDHDYIDRYTLGFDQLQQRVAQWTPARTAEVCGISAEQVQELAREY 283 L G++H+ + EDL+D DYI +T GF +L+Q V ++TP AE+CGI+ + + AR + Sbjct: 248 LFNGMLHICLWEDLVDVDYIAAHTEGFAELKQTVREYTPKMVAEICGINEADLMQAARWF 307 Query: 284 GRVALAGEGVFIRANYGLQRVRGGGMAARNVACLPALVGAWRHAAGGVQLSVSDSFPKDM 343 G+ A A ++ + GL + G A+N A + + + G P M Sbjct: 308 GQ-AKAALSMYCQ---GLNQSSSG--TAKNAALINLHLATHQIGKPGAGPFSLTGQPNAM 361 Query: 344 AALSRPDLAAR------HGQPSRVINMSTIGDDLLRPASPAFGP----------KVEALI 387 LA G P ++ + P P +++ + Sbjct: 362 GGREVGGLATMLSGHRDLGNPQHRAEIAALWGVDAVPEKPGKSAVEMFEAVRTGEIKLIW 421 Query: 388 VYNSNPVAVAPESGKVAQGFAREDLFTVVLEHFQTDTADYADILLPATTQLEHIDVHASY 447 + +NP PE + + +L V + T + DYAD+LLPA+T E + + Sbjct: 422 IVCTNPAQSMPEQAMIREALRNAELVVVQEAYRTTASCDYADVLLPASTWSEK-EGTVTN 480 Query: 448 GHVYIIANNAAIAPMGEAKPNTEI----FRLLAAAMGFTEDCFKDSDDQLAAVAFRRDDP 503 I + + + E++ + EI R L A MG + F + + R+ Sbjct: 481 SERRITRFKSVLPKLAESRHDWEIAVEFARKLEAVMGKDKSLFPFAGTE-EIWNEHREST 539 Query: 504 RSGDYDWEQLKQKGWQRLAVPVAPFAEGGFATPSGKCEFYSEQMKAAGLDPLPTYIPPYE 563 R D D L K + P+ EG +GK Y + G+ P + + Sbjct: 540 RGRDLDITGLSFKILEEQGPQQWPYPEGA---TTGKKRLYED-----GIFPTESGRARFV 591 Query: 564 SVASNP---QLGAKYPLAMISPPARNFLN--SSFVNVQSLRDTEGEPHLDMHPQDAAARG 618 + P ++ A+YP + + R+ + S V L EP + M D R Sbjct: 592 NTVYQPVADRVDARYPFHLTTGRLRDQWHGMSRTGTVAQLFAHAAEPAVAMSKTDMERRQ 651 Query: 619 IVNGDTVRIFNDRGAMQARVRVTDKARPGLVVGLSIWWKKFAA------DGKNA-NEVTS 671 + NGD V + + G+ V + + R G V W +++ + DG + N +TS Sbjct: 652 LHNGDLVHVTSKHGSQIFPVALGEDMRAGQVFVGMHWGEEYVSGRGRDGDGSHGVNALTS 711 Query: 672 QRLTDMGNAPTFYDVLVQVEKLPQP 696 P V++ K P Sbjct: 712 PAYDPSSKQPELKHAAVKILKAEFP 736 >gi|152981034|ref|YP_001352093.1| formate dehydrogenase alpha subunit [Janthinobacterium sp. Marseille] Length = 946 Score = 136 bits (343), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 177/744 (23%), Positives = 295/744 (39%), Gaps = 113/744 (15%) Query: 6 VRAACPHDCPDTCALLVTVKDGVATEVRGDPDHPPTSGVLCTKVSRYAERTYHQDRLLHP 65 V + CP+ C C L +KD V G D P LC K + H RL P Sbjct: 229 VDSVCPY-CGVGCQLTYNIKDNKILYVEGR-DGPANRERLCVKGRYGFDYANHPHRLTKP 286 Query: 66 M-RRVGKKGEGKF----ERI-------SWEQAISTIAQRLGEIARRDPQAILPYSYAGTM 113 + RR G F +R+ +WE+A+ +I RD S AG Sbjct: 287 LIRRADAPKSGDFIMDPDRVMEVFREATWEEALEVAGGNFAKI--RDTHG--KKSLAG-F 341 Query: 114 GQVQGESMAGRFFNRL-----GASQLERT--ICSSAGGAGYRYTIGT-RVGSDLEQVDHA 165 G +G + F +L G++ ++ +C ++ IG+ V + + V A Sbjct: 342 GSAKGSNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVVALLEGIGSGAVSNPVMDVTKA 401 Query: 166 KLILIWGGNPIASNLHFWTRVQAAKRNGARLIAIDPYRSLTAEKCHQHIALLPGTDAALA 225 ++++I G NP ++ T ++ A NG +LI DP R+ + H+ + TD A+ Sbjct: 402 EVVIIIGANPTVNHPVAATWIKNAVSNGTKLIVCDPRRTEMSRIAHRFMQFKADTDVAML 461 Query: 226 LGLMHVLIAEDLLDHDYIDRYTLGFDQLQQRVAQWTPARTAEVCGISAEQVQELAREYGR 285 +M+V++ E L+D ++I+ T+G+++L++ V +TP A +CGI AE ++ +AR Y Sbjct: 462 NAMMNVIVTEGLVDKEFIESRTIGYEELRKNVEGYTPELMAPICGIDAETLRYVARLYA- 520 Query: 286 VALAGEGVFIRANYGLQRVRGGGMAARNVACLPALVGAWRHAAGGVQ--------LSVSD 337 +G I G+ + G AR + L + G G+ SD Sbjct: 521 ---TSKGSMILWGMGVSQHIHGTDNARCLIALALMTGQIGRPGTGLHPLRGQNNVQGASD 577 Query: 338 S------FPKDMAALSRPDLAARHGQPSRVINMSTIGDDLLRPASPAF----------GP 381 + +P D ++S P + A ++ NMS DLL P P Sbjct: 578 AGLIPMVYP-DYQSVSDPKIRANF---AKAWNMSV---DLLDP-EPGLTVVEIMHAITDG 629 Query: 382 KVEALIVYNSNPVAVAPESGKVAQGFAREDLFTVVLEHFQTDTADYADILLPATTQLEHI 441 K+ L V NP P++ + A D VV + F T+TA AD++LPA+ E Sbjct: 630 KIRGLYVQGENPAMSDPDANHAREALAALDHL-VVQDIFLTETAYLADVILPASAFPEKT 688 Query: 442 DVHASYGHVYIIANNAAIAPMGEAKPNTEIFRLLAAAMGFTEDCFKDSDDQLAAVA---- 497 + + A+ P GEAK + I + +A +G D Q V+ Sbjct: 689 GTFTNTDRTVQMGRQ-ALNPPGEAKQDLWIIQQMANRLGL--------DWQYKHVSEVFD 739 Query: 498 -FRRDDPRSGDYDWEQLKQKGWQRLAVPVAPFAEGG--------FATPSGKCEFYSEQMK 548 R P W++L+++ + P + G F SG+ F + Sbjct: 740 EMRHTMPSIAGITWDRLERE--DSVTYPCEKEGDPGEPVVFVDSFPRESGRARFVPADII 797 Query: 549 AAGLDPLPTYIPPYESVASNPQLGAKYPLAMISPPARNFLNSSFVNVQS--LRDTEGEPH 606 A P P +YP+ +I+ ++ + ++ L E +P Sbjct: 798 PAAERPDP-----------------EYPMVLITGRQLEHWHTGSMTRRATVLDSIEPDPI 840 Query: 607 LDMHPQDAAARGIVNGDTVRIFNDRGAMQARVRVTDKARPGLVVGLSIWWKKFAADGKNA 666 +HP D A G GD + + + RG + R D + G V + F Sbjct: 841 ALIHPLDLANMGGKPGDIITLQSRRGEVSLYARADDGSPRGAV------FVPFCYYEAAI 894 Query: 667 NEVTSQRLTDMGNAPTFYDVLVQV 690 N +T+ L G P F +++ Sbjct: 895 NRLTNAALDPFGKIPEFKYCAIRI 918 >gi|152981248|ref|YP_001355342.1| NAD-dependent formate dehydrogenase alpha subunit [Janthinobacterium sp. Marseille] Length = 957 Score = 115 bits (288), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 170/727 (23%), Positives = 282/727 (38%), Gaps = 91/727 (12%) Query: 14 CPDTCALLVTVKDGVATEVRGDPDHPPTSGVLCTKVSRYA-ERTYHQDRLLHPMRRVGKK 72 C C+ +K + +PD G C K R+A H+DR+L PM R K Sbjct: 247 CGVGCSFKAEMKGNEVVRMVPNPDGKANQGHACVK-GRFAWGYATHKDRMLKPMIR--SK 303 Query: 73 GEGKFERISWEQAISTIAQRLGEIARRDPQAILPYSYAGTMGQVQGESMAGRFFNRL--- 129 ++ +SW++AI+ A L I QA G + + + G +L Sbjct: 304 ITDPWKEVSWDEAINYAASELKRI-----QAKYGKDSIGGLVSSRCTNEEGYLVQKLVRA 358 Query: 130 --GASQLERT--ICSSAGGAGYRYTIGTRVGSD-LEQVDHAKLILIWGGNPIASNLHFWT 184 G + ++ +C S G G + T+G G+ E V + +IL+ G NP + + F + Sbjct: 359 AFGNNNVDTCARVCHSPTGYGLKVTMGESAGTQTFESVMKSDVILVIGANPASGHPVFAS 418 Query: 185 RVQAAKRNGARLIAIDPYRSLTAEKCH----QHIALLPGTDAALALGLMHVLIAEDLLDH 240 +++ R GA+LI +DP + + H H+ L PG++ A+ L HV+I E+L Sbjct: 419 QMKRRIRQGAKLIVVDPRTTEMVKSPHIQADYHLKLKPGSNVAIVNALAHVIITENLHKP 478 Query: 241 DYIDRYTL--GFDQLQQRVA--QWTPARTAEVCGISAEQVQELAREYGRVALAGEGVFIR 296 D+I F++ + V+ + +P EV G+ A ++ AR + G I Sbjct: 479 DFIAARCEVDSFEEWAEFVSLPENSPEAMEEVSGVPAADIRGAARLFA----TGGNAAIY 534 Query: 297 ANYGLQRVRGGGMAARNVACLPALVGAWRHAAGGV-----QLSVS-----DSFPKDMAAL 346 G+ G +A L G GV Q +V SFP ++ Sbjct: 535 YGLGVTEHAQGSTTVIGIANLAMATGNIGRDGVGVNPLRGQNNVQGSCDMGSFPHELPGY 594 Query: 347 SRPDLAARHGQPSRVINMSTIGDDLLRPAS---PAFGPKVEALIVYNSNPVAVAPESGKV 403 + + V + + LR + A L + V P + V Sbjct: 595 RHVSDSVARAEFEAVWGCTLESEPGLRIPNMFDAAMDGTFMGLYCEGEDIVQSDPNTQHV 654 Query: 404 AQGFAREDLFTVVLEHFQTDTADYADILLPATTQLEHIDVHASYGHVYIIANNAAIAPMG 463 + +V + F +TA YA + LP ++ LE + I+ + P Sbjct: 655 TAALEAMECI-IVQDIFLNETAKYAHVFLPGSSFLEKDGTFTNAERR--ISRVRKVMPPK 711 Query: 464 EAKPNTEIFRLLAAAMGFTEDCF--KDSDDQLAA-------VAFRRDDPRSGDYDWEQLK 514 + E+ ++L+ A+G+ + ++ D++AA V+F+R D G W Sbjct: 712 SGYGDWEVTQMLSNALGYPMNYKHPREIMDEIAALTPTFTGVSFKRIDELGGSIQW---- 767 Query: 515 QKGWQRLAVPVAPFAEGGFATPSGKCEFYSEQMKAAGLDPLPTYIPPYESVASNPQLGAK 574 P AE TP+ E E ++ G Y E V K Sbjct: 768 ---------PCN--AEAPEGTPTMHTE---EFVRGKGRFINTKYFGAGEKV------NKK 807 Query: 575 YPLAMISPPARNFLNSSFVNVQSLRDTEG----EPHLDMHPQDAAARGIVNGDTVRIFND 630 +PL + + L+ V Q+ R E L++HP DA RGI D V I + Sbjct: 808 FPLILTTG---RILSQYNVGAQTRRTFNNMWHSEDRLEIHPHDAEERGIKQDDWVGIESR 864 Query: 631 RGAMQARVRVTDKARPGLVVGLSIWWKKFAADGKNANEVTSQRLTDMGNAPTFYDVLVQV 690 G R +T++ +PG+V + F AN +T+ N P + VQV Sbjct: 865 AGQTVLRADITERIQPGVV------YTTFHFPESGANVITTNSSDWATNCPEYKVTAVQV 918 Query: 691 EKLPQPA 697 + QP+ Sbjct: 919 MPVAQPS 925 >gi|152982393|ref|YP_001353660.1| formate dehydrogenase large subunit precursor [Janthinobacterium sp. Marseille] Length = 987 Score = 105 bits (263), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 74/282 (26%), Positives = 134/282 (47%), Gaps = 15/282 (5%) Query: 5 IVRAACPHDCPDTCALLVTVKDGVATEVRGDP--DHPPTSGVLCTKVSRYAERTYHQDRL 62 + R C H C CA V++GV VR +P D P G C K + E + + RL Sbjct: 76 VKRTVCTH-CSVGCASDAIVENGVW--VRQEPVFDSPINLGAHCAKGAALREHGHGEYRL 132 Query: 63 LHPMRRVGKKGEGKFERISWEQAISTIAQRLGEIARRD-PQAILPYSYAGTMGQVQGESM 121 +PM+ V GK++RISW+QA++ I+ ++ +I + P ++ Y + + ++ Sbjct: 133 KYPMKLV----NGKYQRISWDQALNEISAKMLDIRKASGPDSV--YFVGSSKHNNEQAAL 186 Query: 122 AGRFFNRLGASQLERT--ICSSAGGAGYRYTIGT-RVGSDLEQVDHAKLILIWGGNPIAS 178 +F + G + + IC S AG T G + + + ++K L G N + Sbjct: 187 MRKFVSFFGTNNTDHQARICHSTTVAGVANTWGYGAMTNSYNDMQNSKAALYIGSNAAEA 246 Query: 179 NLHFWTRVQAAKRNGARLIAIDPYRSLTAEKCHQHIALLPGTDAALALGLMHVLIAEDLL 238 + + AK G ++I +DP + TA K Q+I + G+D G+++ + Sbjct: 247 HPVSMLHMLHAKETGTKMIVVDPRYTRTAAKADQYIRIRSGSDIPFLWGVLYHIFKNGWE 306 Query: 239 DHDYIDRYTLGFDQLQQRVAQWTPARTAEVCGISAEQVQELA 280 D YI+ G +++++ V +WTP + E CG+ ++ + A Sbjct: 307 DKKYINDRVYGMEKVREEVMKWTPEKVEEACGVPEAEIYKAA 348 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,758,660 Number of extensions: 77570 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 4 Length of query: 700 Length of database: 1,221,021 Length adjustment: 90 Effective length of query: 610 Effective length of database: 888,291 Effective search space: 541857510 Effective search space used: 541857510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)