BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3733.p (471 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152979945|ref|YP_001352025.1| glycolate oxidase (fad-linked s... 229 3e-61 gi|152980854|ref|YP_001353872.1| FAD/FMN-containing dehydrogenas... 180 1e-46 gi|152981534|ref|YP_001352125.1| glycolate oxidase subunit [Jant... 120 1e-28 gi|152981491|ref|YP_001354804.1| (S)-2-hydroxy-acid oxidase [Jan... 66 6e-12 >gi|152979945|ref|YP_001352025.1| glycolate oxidase (fad-linked subunit) oxidoreductase protein [Janthinobacterium sp. Marseille] Length = 495 Score = 229 bits (583), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 141/458 (30%), Positives = 232/458 (50%), Gaps = 14/458 (3%) Query: 16 QALLDELKAVFGERCSTTLAMREHHGR----DESSYDPMLPDAVVFAHSTEEVATFVKLC 71 + ++D L+AV ERC + E R D + LP VV + E+V +K+C Sbjct: 15 RTVVDALRAVLPERC---VLFNEEDTRPYECDGLAAYAQLPMVVVLPDTEEQVIAALKVC 71 Query: 72 SQYDTPIIPYGAGTSLEGHVLALQGGVTVDLSQMNQVLAVNAEDLTATVQAGVTRKQLNQ 131 + PI+P GAGT L G L + G+ + +++N+++ V+A TA VQ GV +++ Sbjct: 72 NALKVPIVPRGAGTGLSGGALPIADGIVLSTARLNKIVKVDAYARTAVVQPGVRNLAISE 131 Query: 132 EIKDTGLFFPIDPGAD--ASLGGMASTRASGTNAVRYGTMKENTLTLTVVTAQGEIIKTG 189 + GL++ DP + ++GG + + G + ++YG N + + VVT +G++I+ G Sbjct: 132 AVAPLGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVMRVRVVTIEGDVIELG 191 Query: 190 TRAKKSSAGYDLTRVYVGSEGTLGIITEVTVRLYPQPEAISAAICSFPSVADAVNTVIQT 249 A + G DL V++GSEG LG++TEVTV+L P+P+ + SF V N V Sbjct: 192 N-AALDAPGLDLLSVFIGSEGMLGMVTEVTVKLIPKPKLARVILASFDDVVKGGNAVASV 250 Query: 250 IQMGVPLARVELLDENGVRAINAHDKLSLPVNP--LLLFEFHGSDNGVKEQAELVQDIAH 307 I G+ A +E++D+ R + K ++ +LL E G V E+ + ++ Sbjct: 251 IAAGIIPAGLEMMDKTSSRMVEPFVKAGYDIDAEAILLCESDGIPEEVDEEIARMTEVLK 310 Query: 308 ENHARGFEWATRPEDRSRLWTARHNAYFALLQLRPGARAISTDCCVPISRLAECVLATKA 367 + A + +R R W+ R NA+ A ++ P I D +P LA+ +L Sbjct: 311 HSGATAIAVSQNEAERLRFWSGRKNAFPAAGRISPDYYCI--DGTIPRRSLADVLLGIAQ 368 Query: 368 DCEEQGLTHAIIGHVGDGNFHVQMMVDPNDPADIARAEGVNARMVARAIGMDGTCTGEHG 427 + GL A + H GDGN H ++ D N + RAE A ++ + + GT TGEHG Sbjct: 369 MEVKYGLRCANVFHAGDGNLHPLILFDANQEGEFHRAELFGAEILEMCVAVGGTITGEHG 428 Query: 428 VGLHKMDFLVQEHGEGAIAVMRAIKHALDPKNIMNPGK 465 VGL K++ + + I A+K A DP +++NP K Sbjct: 429 VGLEKINQMCVQFSRAEIDAFLAVKRAFDPVSLLNPTK 466 >gi|152980854|ref|YP_001353872.1| FAD/FMN-containing dehydrogenase [Janthinobacterium sp. Marseille] Length = 468 Score = 180 bits (457), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 138/432 (31%), Positives = 218/432 (50%), Gaps = 22/432 (5%) Query: 54 AVVFAHSTEEVATFVKLCSQYDTPIIPYGAGTSLEGHVLALQGGVT--VDLSQMNQVLAV 111 AV+ T+E+AT V+LC++Y P++P G T L + + G + L+++N++ V Sbjct: 41 AVLKPADTQELATLVRLCNEYKVPMVPQGGNTGLVLGGIPDESGTAAILSLTRLNRIRHV 100 Query: 112 NAEDLTATVQAGVTRKQLNQEIKDTGLFFPIDPGADAS--LGGMASTRASGTNAVRYGTM 169 + + T TV+AG K + D G FP+ A+ S +GG ST A GT +RYG Sbjct: 101 DPLNNTVTVEAGCILKNIQAAASDAGRLFPLSLAAEGSCTIGGNLSTNAGGTAVLRYGNT 160 Query: 170 KENTLTLTVVTAQGEIIKTGTRAKKSSAGYDLTRVYVGSEGTLGIITEVTVRLYPQPEAI 229 +E L L VVT QG+I +K + GYDL +Y+G+EGTLG IT ++L+PQP A Sbjct: 161 RELCLGLEVVTPQGDIWHGLRGLRKDNTGYDLRDLYIGAEGTLGFITAAVLKLFPQPRAQ 220 Query: 230 SAAICSFPSVADAVNTVIQTIQMGVP-LARVELLDENGVRAINAH-DKLSLPV---NP-L 283 A+ + + DA+ + V L EL+ +R +N H + LP +P Sbjct: 221 LTALAALRTPDDALRLLSLAQSYCVSALTGFELMSGFCLRLVNRHFPDMRLPFAEEHPQY 280 Query: 284 LLFEFHGSDNGVKEQAELVQDIAHENHARGFEWA--TRPEDRSR-LWTARHNAYFALLQL 340 +L E S++ Q L I+ + A R +S+ +W R + L Q Sbjct: 281 VLLELSDSESEEHAQTMLEHVISTAMQQDLIQDAIVARSLAQSKAIWDIRE--HIPLAQA 338 Query: 341 RPGARAISTDCCVPISRLAECVLATKADCEEQ--GLTHAIIGHVGDGNFHVQMM--VDPN 396 G + I D +PISR+A+ + T A ++ G GH+GDGN H + Sbjct: 339 AEG-KNIKHDVSLPISRIADFIRTTDALLQQAFPGARMVTFGHLGDGNLHYNVAHPEQET 397 Query: 397 DPADIARAEGVNARMVARAIGMD-GTCTGEHGVGLHKMDFLVQEHGEGAIAVMRAIKHAL 455 D + + + VN R+V ++ G+ + EHG+G K +++ + + +MR IK AL Sbjct: 398 DQSFLQKQTAVN-RVVHDSVHQHGGSISAEHGIGALKRTEILRYKSDVEMQLMRTIKQAL 456 Query: 456 DPKNIMNPGKIL 467 DP N+MNPGK++ Sbjct: 457 DPLNLMNPGKLI 468 >gi|152981534|ref|YP_001352125.1| glycolate oxidase subunit [Janthinobacterium sp. Marseille] Length = 1000 Score = 120 bits (302), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 124/505 (24%), Positives = 209/505 (41%), Gaps = 81/505 (16%) Query: 35 AMREHHGRDESSYDPMLPDAVVFAHSTEEVATFVKLCSQYDTPIIPYGAGTSLEGHVLAL 94 A R + D S Y ++P VV +++ + + TPI+ G GTS G + Sbjct: 36 ADRGRYATDASIYQ-IMPIGVVVPRDQDDLLLCLDIARSEKTPILARGGGTSQCGQTVG- 93 Query: 95 QGGVTVDLSQ-MNQVLAVNAEDLTATVQAGVTRKQLNQEIKDTGLFFPID--PGADASLG 151 + +D S+ +N V+ + + T TV+ G+ LN +K GL+FP+D A ++G Sbjct: 94 -EALVIDNSKWLNNVVDFDPIERTITVEPGIVLDHLNAWLKPHGLWFPVDVSTAAQCTIG 152 Query: 152 GMASTRASGTNAVRYGTMKENTLTLTVVTAQG---------------------------- 183 GMA + G+ ++ YG M N L + V A G Sbjct: 153 GMAGNNSCGSRSIEYGNMVHNVLAIDAVLADGTQARFETLEKMAANARLQEITQGLRRIA 212 Query: 184 -----EIIKTGTRAKKSSAGY-------------------DLTRVYVGSEGTLGIITEVT 219 EII+ + + GY +L + VGSEGTL ++ Sbjct: 213 TREQDEIIERVPKVLRRVGGYNIDLFDCQNPRAYTDDGSANLAHILVGSEGTLAFSRQLK 272 Query: 220 VRLYPQPEAISAAICSFPSVADAVNTVIQTIQMGVPLARVELLDENGVRAINAHDKLSLP 279 ++L P P+ + +FP+ A+++ +++ +P A VEL+D ++ ++ ++ P Sbjct: 273 LKLSPLPKHKVLGVINFPTFYQAMDSAQHIVKL-LPTA-VELVDSTMIK-LSLNNPAFRP 329 Query: 280 V---------NPLLLFEFHGSDN-GVKEQAELVQDIAHENHARG--FEWATRPEDRSRLW 327 V +LL EF G D + E+ + D+ + G + E +S LW Sbjct: 330 VIEKALVGTPEAILLVEFSGDDKAALVERLSALSDLMSDLSLPGSVVDMVDAGEQKS-LW 388 Query: 328 TARHNAYFALLQLRPGARAIS--TDCCVPISRLAECVLATKADCEEQGLTHAIIGHVGDG 385 R ++ ++ + +S DC VP+ LAE + H G Sbjct: 389 DVRKAGLNIMMSMKGDGKPVSFIEDCAVPLVHLAEYTSRLTEVFHKYHTEGTWYAHASVG 448 Query: 386 NFHVQMMVD--PNDPADIARAEGVNARMVARAIGMDGTCTGEHGVGLHKMDFLVQEHGEG 443 HV+ ++D N AD+ R A + R G +GEHG GL + +++ ++G Sbjct: 449 TLHVRPILDMRRNGAADM-RQIAEEASALVRE--YKGAYSGEHGDGLCRGEWVSWQYGPK 505 Query: 444 AIAVMRAIKHALDPKNIMNPGKILR 468 IK DP N NP KI+R Sbjct: 506 INQAFSEIKDLFDPDNRFNPDKIVR 530 >gi|152981491|ref|YP_001354804.1| (S)-2-hydroxy-acid oxidase [Janthinobacterium sp. Marseille] Length = 1332 Score = 65.9 bits (159), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 100/397 (25%), Positives = 154/397 (38%), Gaps = 77/397 (19%) Query: 37 REHHGRDESSYDPMLPDAVVFAHSTEEVATFVKLCSQYDTPIIPYGAGTSLEGHVLALQG 96 R H D + + P V+ S +E+A VK C + IIP G GT G + L Sbjct: 179 RVSHVTDATDWRVEYPFVVLTPDSEDEMAGLVKGCIELGLTIIPRGGGTGYTGGAVPLTP 238 Query: 97 GVTV---------DLSQMNQVLAVNAEDLTATVQAGVTRKQLNQEIKDTGLFFPIDP-GA 146 V +M + V+ E T AGV K+++ + G F +DP A Sbjct: 239 MSAVINTEKLEALGAVEMTMLPGVDREYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSA 298 Query: 147 DAS-LGGMASTRASGTNAVRYGTMKENTLTLTVVTAQGEIIKT----------------- 188 +AS +GG + A G AV +GT +N + +V G+ ++ Sbjct: 299 EASCIGGNIAMNAGGKKAVLWGTALDNLASWRMVDPNGDWLEVTRLEHNLGKIHDAPVAK 358 Query: 189 --------------------------GTRAKKSSAGYDLTRVY------VGSEGTLGIIT 216 G +K G D+T + V EG G+IT Sbjct: 359 FKLEWTHPAEKGRVNQPFKSETLEIAGRTFRKEGLGKDVTDKFLSGLPGVQKEGCDGLIT 418 Query: 217 EVTVRLYPQPEAISAAIC--SFPSVADAVNTVI--------QTIQMGVPLARVELLDENG 266 L+ P+ + +C F DA+ +++ +T + G LA +E LDE Sbjct: 419 SARWILHKMPKH-TRTVCLEFFGHSRDAIPSIVEIKDFLDAETKKGGAILAGLEHLDERY 477 Query: 267 VRAINAHDKLSLPVNP-LLLF-EFHGSD-NGVKEQAELVQDIAHENHARGFEWATRPEDR 323 +RA+ K + P + LF + G D N V + A V +A+ GF A PE R Sbjct: 478 LRAVGYATKSKRGILPKMALFGDIVGDDENAVAQAASEVVRLANTRVGEGFV-AVSPEAR 536 Query: 324 SRLWTARHNAYFALLQLRPGARAISTDCCVPISRLAE 360 + W R A A + A I+ D +P+ R+ E Sbjct: 537 KKFWLDR--ARTAAISKHTNAFKINEDVVIPLDRMGE 571 Score = 58.5 bits (140), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 29/87 (33%), Positives = 49/87 (56%), Gaps = 4/87 (4%) Query: 381 HVGDGNFHVQMMVDPNDPADIARAEGVNARMVARAIGMDGTCTGEHGVGLHKMDFLVQEH 440 H GDGN H + V+ + + A AR++A A ++G +GEHG+G+ K++FL Sbjct: 739 HAGDGNVHTNIPVNSDHYQMLQDAHAAVARIMALARSLNGVISGEHGIGITKLEFLT--- 795 Query: 441 GEGAIAVMRAIKHALDPKNIMNPGKIL 467 + +A R+ K +DP+ N GK++ Sbjct: 796 -DAELADFRSYKRRIDPEGRFNKGKLM 821 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.318 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,113,556 Number of extensions: 45880 Number of successful extensions: 160 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 7 Length of query: 471 Length of database: 1,221,021 Length adjustment: 87 Effective length of query: 384 Effective length of database: 899,382 Effective search space: 345362688 Effective search space used: 345362688 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)