BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3731.p (495 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152979945|ref|YP_001352025.1| glycolate oxidase (fad-linked s... 854 0.0 gi|152980854|ref|YP_001353872.1| FAD/FMN-containing dehydrogenas... 154 1e-38 gi|152981534|ref|YP_001352125.1| glycolate oxidase subunit [Jant... 129 3e-31 gi|152981491|ref|YP_001354804.1| (S)-2-hydroxy-acid oxidase [Jan... 71 1e-13 >gi|152979945|ref|YP_001352025.1| glycolate oxidase (fad-linked subunit) oxidoreductase protein [Janthinobacterium sp. Marseille] Length = 495 Score = 854 bits (2207), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/495 (83%), Positives = 450/495 (90%) Query: 1 MNAPAQPSFAPARQREVVDALRRVVPAHCVLFDEEDTRPYECDGLAAYRQLPMVVVLPET 60 MN + P F A QR VVDALR V+P CVLF+EEDTRPYECDGLAAY QLPMVVVLP+T Sbjct: 1 MNTTSAPPFTAAYQRTVVDALRAVLPERCVLFNEEDTRPYECDGLAAYAQLPMVVVLPDT 60 Query: 61 EAQVVAILRTCHALQVQIVPRGAGTGLSGGAMPIADGVVLSTAKFNQILKMDKYSRTAVV 120 E QV+A L+ C+AL+V IVPRGAGTGLSGGA+PIADG+VLSTA+ N+I+K+D Y+RTAVV Sbjct: 61 EEQVIAALKVCNALKVPIVPRGAGTGLSGGALPIADGIVLSTARLNKIVKVDAYARTAVV 120 Query: 121 QPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVLRVRM 180 QPGVRNLAISEA AP GLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNV+RVR+ Sbjct: 121 QPGVRNLAISEAVAPLGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVMRVRV 180 Query: 181 VTIEGDVVELGSGALDAPGLDLLAVFIGSEGMLGVVTEVTVKLVPKPQAARVIMASFDDV 240 VTIEGDV+ELG+ ALDAPGLDLL+VFIGSEGMLG+VTEVTVKL+PKP+ ARVI+ASFDDV Sbjct: 181 VTIEGDVIELGNAALDAPGLDLLSVFIGSEGMLGMVTEVTVKLIPKPKLARVILASFDDV 240 Query: 241 VKGGNAVANVIAAGIIPAGLEMMDKTSSRMVEPFVKAGYDIDAEAILLCESDGTTEEVEE 300 VKGGNAVA+VIAAGIIPAGLEMMDKTSSRMVEPFVKAGYDIDAEAILLCESDG EEV+E Sbjct: 241 VKGGNAVASVIAAGIIPAGLEMMDKTSSRMVEPFVKAGYDIDAEAILLCESDGIPEEVDE 300 Query: 301 EIGRMTDVLNASGATAIACSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKQLA 360 EI RMT+VL SGATAIA SQ+EAERLRFWSGRKNAFPAAGRISPDYYC+DGTIPR+ LA Sbjct: 301 EIARMTEVLKHSGATAIAVSQNEAERLRFWSGRKNAFPAAGRISPDYYCIDGTIPRRSLA 360 Query: 361 QVLLGIAEMEKKYGLRCANVFHAGDGNLHPLILFDANLADEFHRAELFGAEILELCVEVG 420 VLLGIA+ME KYGLRCANVFHAGDGNLHPLILFDAN EFHRAELFGAEILE+CV VG Sbjct: 361 DVLLGIAQMEVKYGLRCANVFHAGDGNLHPLILFDANQEGEFHRAELFGAEILEMCVAVG 420 Query: 421 GTITGEHGVGIEKINSMCVQFSPAERESFFKLKRAFDPPFLLNPDKAIPTLHRCAEYGKM 480 GTITGEHGVG+EKIN MCVQFS AE ++F +KRAFDP LLNP KAIPTL RCAEYGKM Sbjct: 421 GTITGEHGVGLEKINQMCVQFSRAEIDAFLAVKRAFDPVSLLNPTKAIPTLQRCAEYGKM 480 Query: 481 HVQRGQLRFPDLPRF 495 HV+RG L+FPDLPRF Sbjct: 481 HVKRGMLKFPDLPRF 495 >gi|152980854|ref|YP_001353872.1| FAD/FMN-containing dehydrogenase [Janthinobacterium sp. Marseille] Length = 468 Score = 154 bits (389), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 134/475 (28%), Positives = 218/475 (45%), Gaps = 35/475 (7%) Query: 18 VDALRRVVPAHCVLFDEEDTRPYECDGLAAYRQLPMVVVLPETEAQVVAILRTCHALQVQ 77 +DA RR + A VL + DT PY D + V+ P ++ ++R C+ +V Sbjct: 5 LDACRRAIGATHVLTEAADTAPYLTDWRRRFTGKARAVLKPADTQELATLVRLCNEYKVP 64 Query: 78 IVPRGAGTGLSGGAMPIADGV--VLSTAKFNQILKMDKYSRTAVVQPGVRNLAISEAAAP 135 +VP+G TGL G +P G +LS + N+I +D + T V+ G I AA+ Sbjct: 65 MVPQGGNTGLVLGGIPDESGTAAILSLTRLNRIRHVDPLNNTVTVEAGCILKNIQAAASD 124 Query: 136 HGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVLRVRMVTIEGDVVE-LGSGA 194 G + +++ +CTIGGN++ N+GG L+YG T L + +VT +GD+ L Sbjct: 125 AGRLFPLSLAAEGSCTIGGNLSTNAGGTAVLRYGNTRELCLGLEVVTPQGDIWHGLRGLR 184 Query: 195 LDAPGLDLLAVFIGSEGMLGVVTEVTVKLVPKPQAARVIMASF---DDVVKGGNAVANVI 251 D G DL ++IG+EG LG +T +KL P+P+A +A+ DD ++ + + Sbjct: 185 KDNTGYDLRDLYIGAEGTLGFITAAVLKLFPQPRAQLTALAALRTPDDALRLLSLAQSYC 244 Query: 252 AAGIIPAGLEMMDKTSSRMVE--------PFVKAGYDIDAEAILLCESDGTTEE-----V 298 + + G E+M R+V PF + + +LL SD +EE + Sbjct: 245 VSAL--TGFELMSGFCLRLVNRHFPDMRLPFAEE----HPQYVLLELSDSESEEHAQTML 298 Query: 299 EEEIGR-MTDVLNASGATAIACSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRK 357 E I M L A + +QS+A W R++ P A D ++P Sbjct: 299 EHVISTAMQQDLIQDAIVARSLAQSKA----IWDIREH-IPLAQAAEGKNIKHDVSLPIS 353 Query: 358 QLAQVLLGI-AEMEKKY-GLRCANVFHAGDGNLHPLILFDANLADE--FHRAELFGAEIL 413 ++A + A +++ + G R H GDGNLH + D+ + + Sbjct: 354 RIADFIRTTDALLQQAFPGARMVTFGHLGDGNLHYNVAHPEQETDQSFLQKQTAVNRVVH 413 Query: 414 ELCVEVGGTITGEHGVGIEKINSMCVQFSPAERESFFKLKRAFDPPFLLNPDKAI 468 + + GG+I+ EHG+G K + S E + +K+A DP L+NP K I Sbjct: 414 DSVHQHGGSISAEHGIGALKRTEILRYKSDVEMQLMRTIKQALDPLNLMNPGKLI 468 >gi|152981534|ref|YP_001352125.1| glycolate oxidase subunit [Janthinobacterium sp. Marseille] Length = 1000 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 122/507 (24%), Positives = 209/507 (41%), Gaps = 76/507 (14%) Query: 30 VLFDEEDTRPYECDGLAAYRQLPMVVVLPETEAQVVAILRTCHALQVQIVPRGAGTGLSG 89 VLF + D Y D + Y+ +P+ VV+P + ++ L + + I+ RG GT G Sbjct: 31 VLFSKADRGRYATDA-SIYQIMPIGVVVPRDQDDLLLCLDIARSEKTPILARGGGTSQCG 89 Query: 90 GAMPIADGVVLSTAKF-NQILKMDKYSRTAVVQPGVRNLAISEAAAPHGLYYAPDPSSQI 148 + + +V+ +K+ N ++ D RT V+PG+ ++ PHGL++ D S+ Sbjct: 90 --QTVGEALVIDNSKWLNNVVDFDPIERTITVEPGIVLDHLNAWLKPHGLWFPVDVSTAA 147 Query: 149 ACTIGGNVAENSGGVHCLKYGLTVHNVLRV------------------------------ 178 CTIGG NS G ++YG VHNVL + Sbjct: 148 QCTIGGMAGNNSCGSRSIEYGNMVHNVLAIDAVLADGTQARFETLEKMAANARLQEITQG 207 Query: 179 --RMVTIEGD-VVELGSGAL--------------------DAPGLDLLAVFIGSEGMLGV 215 R+ T E D ++E L D +L + +GSEG L Sbjct: 208 LRRIATREQDEIIERVPKVLRRVGGYNIDLFDCQNPRAYTDDGSANLAHILVGSEGTLAF 267 Query: 216 VTEVTVKLVPKPQAARVIMASFDDVVKGGNAVANVIAAGIIPAGLEMMDKTSSRM----- 270 ++ +KL P P+ + + +F + ++ +++ ++P +E++D T ++ Sbjct: 268 SRQLKLKLSPLPKHKVLGVINFPTFYQAMDSAQHIVK--LLPTAVELVDSTMIKLSLNNP 325 Query: 271 -VEPFVKAGYDIDAEAILLCESDGTTEE-VEEEIGRMTDVLNASGATAIACSQSEA-ERL 327 P ++ EAILL E G + + E + ++D+++ +A E+ Sbjct: 326 AFRPVIEKALVGTPEAILLVEFSGDDKAALVERLSALSDLMSDLSLPGSVVDMVDAGEQK 385 Query: 328 RFWSGRKNAF----PAAGRISPDYYCMDGTIPRKQLAQVLLGIAEMEKKYGLRCANVFHA 383 W RK G P + D +P LA+ + E+ KY HA Sbjct: 386 SLWDVRKAGLNIMMSMKGDGKPVSFIEDCAVPLVHLAEYTSRLTEVFHKYHTEGTWYAHA 445 Query: 384 GDGNLH--PLILFDANLADEFHRAELFGAEILELCVEVGGTITGEHGVGIEKINSMCVQF 441 G LH P++ N A + + E L E G +GEHG G+ + + Q+ Sbjct: 446 SVGTLHVRPILDMRRNGAADMRQ---IAEEASALVREYKGAYSGEHGDGLCRGEWVSWQY 502 Query: 442 SPAERESFFKLKRAFDPPFLLNPDKAI 468 P ++F ++K FDP NPDK + Sbjct: 503 GPKINQAFSEIKDLFDPDNRFNPDKIV 529 >gi|152981491|ref|YP_001354804.1| (S)-2-hydroxy-acid oxidase [Janthinobacterium sp. Marseille] Length = 1332 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 112/466 (24%), Positives = 177/466 (37%), Gaps = 83/466 (17%) Query: 21 LRRVVPAHCVLFDEEDTRPYECDGLAAYRQLPMVVVLPETEAQVVAILRTCHALQVQIVP 80 L R + FD + D + P VV+ P++E ++ +++ C L + I+P Sbjct: 163 LGRYTEKDNIKFDGLSRVSHVTDATDWRVEYPFVVLTPDSEDEMAGLVKGCIELGLTIIP 222 Query: 81 RGAGTGLSGGAMPIAD-GVVLSTAKFNQI----LKM----DKYSRTAVVQPGVRNLAISE 131 RG GTG +GGA+P+ V++T K + + M D+ T GV +S+ Sbjct: 223 RGGGTGYTGGAVPLTPMSAVINTEKLEALGAVEMTMLPGVDREYATIYSGAGVVTKRVSD 282 Query: 132 AAAPHGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVLRVRMVTIEGDVVELG 191 AA G +A DP+S A IGGN+A N+GG + +G + N+ RMV GD +E+ Sbjct: 283 AAEKAGFVFAVDPTSAEASCIGGNIAMNAGGKKAVLWGTALDNLASWRMVDPNGDWLEVT 342 Query: 192 --------------------------SGALDAP------------------GLDLLAVFI 207 G ++ P G D+ F+ Sbjct: 343 RLEHNLGKIHDAPVAKFKLEWTHPAEKGRVNQPFKSETLEIAGRTFRKEGLGKDVTDKFL 402 Query: 208 G------SEGMLGVVTEVTVKLVPKPQAARVIMASF----DDVVKGGNAVANVIAA---- 253 EG G++T L P+ R + F D + + + + A Sbjct: 403 SGLPGVQKEGCDGLITSARWILHKMPKHTRTVCLEFFGHSRDAIPSIVEIKDFLDAETKK 462 Query: 254 -GIIPAGLEMMDKTSSRMVEPFVKAGYDIDAEAILLCESDGTTE----EVEEEIGRMTDV 308 G I AGLE +D+ R V K+ I + L + G E + E+ R+ + Sbjct: 463 GGAILAGLEHLDERYLRAVGYATKSKRGILPKMALFGDIVGDDENAVAQAASEVVRLANT 522 Query: 309 LNASGATAIACSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKQLAQVLLGI-- 366 G A+ S R +FW R + + D IP ++ + GI Sbjct: 523 RVGEGFVAV----SPEARKKFWLDRARTAAISKHTNAFKINEDVVIPLDRMGEYSDGIER 578 Query: 367 --AEMEKKYGLRCANVFHA--GDGNLHPLILFDANLADEFHRAELF 408 E+ + L+ H GNL PL D D+ AEL Sbjct: 579 INVELSIRNKLQLLEELHTFFAKGNL-PLGKSDDAEGDDIPAAELL 623 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.321 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,086,633 Number of extensions: 42552 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 5 Length of query: 495 Length of database: 1,221,021 Length adjustment: 87 Effective length of query: 408 Effective length of database: 899,382 Effective search space: 366947856 Effective search space used: 366947856 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)