BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3531.p (531 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980948|ref|YP_001352058.1| glutamate synthase large subuni... 749 0.0 gi|152980727|ref|YP_001354991.1| glutamate synthase (NADPH) larg... 125 5e-30 >gi|152980948|ref|YP_001352058.1| glutamate synthase large subunit [Janthinobacterium sp. Marseille] Length = 540 Score = 749 bits (1933), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/538 (65%), Positives = 428/538 (79%), Gaps = 9/538 (1%) Query: 1 MWANRYFVFWAVVALTLALLALSAAHLLDWGWVLIPLALTAIGIHDLVQQRHAVLRNYPL 60 M+ Y A L + L + H W V++ LT +GI D++Q + AVLRNYP+ Sbjct: 1 MFPIGYLALVASGLLFILSLFFTPQHDSAWWLVVLFGFLTIVGISDVLQAKRAVLRNYPI 60 Query: 61 IGHFRFMFEAIRPELRQYFFEDEMDGRPFSRKKRSVVYQRAKAEVDSRPFGTEIDMQQAG 120 +GHFR+MFE++RPE+RQYF ED+ PFSR +RS+VYQRAK +D RPFGT++D+ Q+ Sbjct: 61 LGHFRYMFESVRPEIRQYFLEDDAAPNPFSRNQRSLVYQRAKQAIDKRPFGTQMDVYQSD 120 Query: 121 HEWIGHSLSPTKIASHDFRVTVGADRAQPYSMSVFNVSAMSFGALSANAIRALNQGAKKG 180 +EWI HS++P K+ SHDFR+T+GA+RAQPYS S+FN+SAMSFGALSANAIRALNQGA+KG Sbjct: 121 YEWINHSIAPAKVVSHDFRITIGAERAQPYSASIFNISAMSFGALSANAIRALNQGAQKG 180 Query: 181 NFAHDTGEGSVSAYHR-----EFGGDLVWQIASGYFGCRNADGSFNEEKFTTQAQSPQIK 235 F HDTGEGS+S YH+ + GGDLVW+I SGYFGCRN DGSF+ EKF AQ PQ+K Sbjct: 181 GFMHDTGEGSISRYHKPDNPNDAGGDLVWEIGSGYFGCRNDDGSFSAEKFALNAQLPQVK 240 Query: 236 MIEVKLSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTPIGLLEFIEK 295 MIEVKLSQGAKPGHGGVLP AK++ EIAA RGIP+GVDC+SP+ HS+F +PIGLL+FIE+ Sbjct: 241 MIEVKLSQGAKPGHGGVLPGAKVSVEIAAARGIPLGVDCVSPSSHSAFDSPIGLLQFIEQ 300 Query: 296 LRSLSAGKPVGIKLCVGHPWEFFSIAKAMLKTGIVPDFITVDGSEGGTGAAPLEFVDHVG 355 LR+LS GKP G KL VGHPWEFF I KAML TGI PDFI +DG EGGTGAAP+EF DHVG Sbjct: 301 LRNLSGGKPTGFKLAVGHPWEFFGIVKAMLATGITPDFIVIDGGEGGTGAAPVEFTDHVG 360 Query: 356 MPLQEGLVMVHNTLVGIGLRDKVKIGASGKIVTAFDVARTLALGADWCNSARGFMFALGC 415 +PLQE L++ HNTLVG LRDKVKIGASGKIV+AFD+ RTLALGADWCNSARGFMFALGC Sbjct: 361 VPLQEALLLAHNTLVGAKLRDKVKIGASGKIVSAFDIVRTLALGADWCNSARGFMFALGC 420 Query: 416 IQSQSCHTDRCPTGVATQNANRARALVVPDKAERVYRFHQSTLHALQELVQAAGLPHTSA 475 IQSQSCHTDRCPTG+ATQ+ R RALVVPDKAERV FH++TL AL EL+ AAGL H S Sbjct: 421 IQSQSCHTDRCPTGIATQDNLRQRALVVPDKAERVANFHRNTLKALAELIAAAGLHHPSE 480 Query: 476 LRPHHIVRRITDHEIQLMSNVLKYLQPNDLIDGNYRYR----VYEKFWPIARAESFHP 529 L+PHH+VRR++ ++++L S +L YL+P L+ R V+ K+WPIA+AESF P Sbjct: 481 LKPHHLVRRVSANQVKLASALLPYLEPGQLLSEANPERELPEVFGKYWPIAQAESFSP 538 >gi|152980727|ref|YP_001354991.1| glutamate synthase (NADPH) large chain [Janthinobacterium sp. Marseille] Length = 1562 Score = 125 bits (315), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 99/328 (30%), Positives = 140/328 (42%), Gaps = 58/328 (17%) Query: 145 DRAQPYSMSV--FNVSAMSFGALSANAIRALNQGAKKGNFAHDTGEG--SVSAYHREF-- 198 D +P V F AMS G++S A L + +TGEG Y E Sbjct: 868 DEVEPAKEIVKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDTKRYRSELRG 927 Query: 199 -------------GGDLVW----------------QIASGYFGCRNADGSFNEEKFTTQA 229 G D+V Q+ASG FG ++ T A Sbjct: 928 IPIKDGSTLASVIGKDVVEVDMPLMPGDSLRSKIKQVASGRFGVTT--------EYLTSA 979 Query: 230 QSPQIKMIEVKLSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTPIGL 289 + QIKM +QGAKPG GG LP K++ IA R GV ISP H + L Sbjct: 980 EQIQIKM-----AQGAKPGEGGQLPGHKVSEYIARLRFSVPGVGLISPPPHHDIYSIEDL 1034 Query: 290 LEFIEKLRSLSAGKPVGIKLCVGHPWEFFSIAKAMLKTGIVP---DFITVDGSEGGTGAA 346 + I L++++ + +KL + + TG+ D + + G +GGTGA+ Sbjct: 1035 AQLIHDLKNVNPSASISVKLVA-------EVGVGTVATGVAKAKADHVVIAGHDGGTGAS 1087 Query: 347 PLEFVDHVGMPLQEGLVMVHNTLVGIGLRDKVKIGASGKIVTAFDVARTLALGADWCNSA 406 PL V G P + GL TL+ GLR ++++ A G++ T DV LGAD + Sbjct: 1088 PLSSVKFAGSPWELGLAETQQTLILNGLRTRIRVQADGQMKTGRDVVIAALLGADEMGFS 1147 Query: 407 RGFMFALGCIQSQSCHTDRCPTGVATQN 434 + GCI + CH + CP GVATQ+ Sbjct: 1148 TAPLVVEGCIMMRKCHLNTCPVGVATQD 1175 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.323 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,249,939 Number of extensions: 53583 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 2 Length of query: 531 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 443 Effective length of database: 895,685 Effective search space: 396788455 Effective search space used: 396788455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)